Biotinylated – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:00 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotinylated – VectorLabs https://staging.vectorlabs.com 32 32 Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-mouse-igg/ Tue, 02 May 2023 17:02:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=14446 Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Mouse
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Product FAQs

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
 

Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Goat Anti-Mouse IgG (H+L) is supplied in solution. With some exceptions the recommended dilution for most applications is 1:200.

(H+L) indicates the antibody recognizes both heavy and light chains.

The post Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-rabbit-igg/ Tue, 02 May 2023 16:58:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=14439 Description

Ready-to-Use (R.T.U.) Biotinylated Goat Anti-Rabbit IgG Secondary Antibody is a stabilized, prediluted, ready-to-use solution of our biotinylated secondary antibodies. It is used in an avidin-biotin or streptavidin-biotin detection system.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Included in the VECTASTAIN® ABC kits

Specifications

 
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Rabbit
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

Ready-to-Use (R.T.U.) Biotinylated Goat Anti-Rabbit IgG Secondary Antibody is a stabilized, prediluted, ready-to-use solution of our biotinylated secondary antibodies. It is used in an avidin-biotin or streptavidin-biotin detection system.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Included in the VECTASTAIN® ABC kits

Specifications

 
Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

The post Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/biotinylated-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 16:55:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=14432 Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits.
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

 

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target Species Universal (Mouse/Rabbit)
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits. 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesUniversal (Mouse/Rabbit)
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.

 
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-rat-igg/ Tue, 02 May 2023 16:52:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14424 Description

R.T.U. Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.  (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L)
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-rat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rat IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Rat
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]>

Description

R.T.U. Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.  (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L) 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-rat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rat IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesRat
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 
These antibody solutions are provided in convenient dropper bottle format for ease of use.

The post Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-goat-igg/ Tue, 02 May 2023 16:50:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=14414 Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L)

Specifications

More Information
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Goat
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L) 

Specifications

More Information
Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesGoat
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-mouse-igg/ Tue, 02 May 2023 16:44:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14403 Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Mouse
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]>

Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.) 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Product FAQs

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays.

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-rabbit-igg/ Tue, 02 May 2023 16:41:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=14396 Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target Species Rabbit
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]>

Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots. 

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications.

This antibody can be detected with the VECTASTAIN® ABC systems or other (strept)avidin reagent

The post Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Goat Anti-Human Kappa Chain Antibody, Biotinylated https://staging.vectorlabs.com/products/biotinylated-goat-anti-human-kappa-chain/ Tue, 02 May 2023 02:29:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=13929 Description

Biotinylated Goat Anti-Human Kappa Chain Antibody is a chain-specific antibody that can be used for tissue and cell staining, ELISAs, and blots. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Features:

  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody

Specifications

Unit Size 0.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage 2–8 °C
Recommended Usage Reconstitute by adding 1.0 ml water. The resulting solution will have the following composition: 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Human
Conjugate Biotinylated
Host Species Goat
Format Concentrate

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Description

Biotinylated Goat Anti-Human Kappa Chain Antibody is a chain-specific antibody that can be used for tissue and cell staining, ELISAs, and blots. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Features:

  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 

Specifications

Unit Size0.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage2–8 °C
Recommended UsageReconstitute by adding 1.0 ml water. The resulting solution will have the following composition: 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesHuman
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. 

Biotinylated Goat Anti-Human Kappa Chain is supplied in solution form. With some exceptions the recommended dilution for most applications is 1:100.

This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies. This biotinylated conjugate can be employed in flow cytometry or used for tissue staining.

The post Goat Anti-Human Kappa Chain Antibody, Biotinylated appeared first on VectorLabs.

]]> Lectin Kit II, Biotinylated https://staging.vectorlabs.com/products/lectin-kit-2-biotinylated/ Sat, 29 Apr 2023 18:26:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=13372

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

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Resources

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
ConjugateBiotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

Documents

Technical Information

These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

]]> M.O.M.® (Mouse on Mouse) Biotinylated Anti-Mouse IgG Reagent https://staging.vectorlabs.com/products/mouse-on-mouse-biotinylated-anti-mouse-ig-reagent/ Thu, 27 Apr 2023 17:59:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=12560 Description

Mouse on Mouse (M.O.M.) Biotinylated Anti-Mouse Ig Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector® M.O.M. Immunodetection Kit components.  This reagent is supplied as 0.1 ml stock solution sufficient to make 25 ml of working solution.

Features of the M.O.M. Immunodetection system:

  • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC or ImmPRESS® reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

Specifications

Unit Size 0.1 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications
Recommended Storage 2-8 °C
Target Species Mouse
Conjugate Biotinylated
Host Species Horse
Format Concentrate

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Description

Mouse on Mouse (M.O.M.) Biotinylated Anti-Mouse Ig Reagent is a specially modified secondary antibody that has been optimized specifically for use with the Vector® M.O.M. Immunodetection Kit components.  This reagent is supplied as 0.1 ml stock solution sufficient to make 25 ml of working solution.

Features of the M.O.M. Immunodetection system:

  • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  • Clear, crisp specific staining of antigens of interest
  • Based on VECTASTAIN® ABC or ImmPRESS® reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

Specifications

Unit Size0.1 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications
Recommended Storage2-8 °C
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesHorse
FormatConcentrate

Documents

Citations

Technical Information

This is the same reagent contained in the Vector M.O.M. Kits (Cat. Nos. PK-2200, FMK-2201, and BMK-2202).

The Vector M.O.M. Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector M.O.M. Kit. The result is clear, crisp specific staining of the antigens of interest.  Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.

The Vector M.O.M. Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

The M.O.M. Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems. Detailed information for multiple antigen labeling can be found in our brochure, “Discovery Through Color: A Guide to Multiple Antigen Labeling”, available on our website.

Four Vector M.O.M. Kits are available. The three kits based on avidin/biotin technology use the same proprietary M.O.M. Mouse Ig Blocking Reagent and the M.O.M. Biotinylated Anti-Mouse Ig Reagent, but offer a choice of an enzyme-based or fluorescent-based visualization method. M.O.M. Mouse Ig Blocking Reagent and M.O.M. Biotinylated Anti-Mouse Ig Reagent are also available separately.

The Vector M.O.M. ImmPRESS Kit contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step, non-biotin M.O.M. ImmPRESS Peroxidase Polymer reagent.

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