Biotin NHS – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:16:33 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin NHS – VectorLabs https://staging.vectorlabs.com 32 32 NHS-biotin https://staging.vectorlabs.com/products/nhs-biotin/ Sat, 24 Feb 2024 02:07:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=35367 NHS-biotin, product number QBD-10205, is a classic, non-dPEG® biotinylation reagent. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin and react s with free primary amines. It is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.

Description

"NHS-biotin, product number QBD-10205, is a classic, non-dPEG® biotinylation reagent. Biotin (5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, CAS number 58-85-5) is a water-soluble vitamin, though it is not especially soluble in water and is used in a wide range of biotechnology and clinical applications. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin. The NHS ester is reactive with free primary amines. It is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.

NHS-biotin, QBD-10205, is insoluble in water, but the NHS ester hydrolyzes if the product gets wet. The optimal storage temperature of NHS-biotin is -20°C. When removed from storage, NHS-biotin should be allowed to equilibrate fully to ambient temperature before opening the bottle. The product should always be handled to minimize exposure to moisture. NHS-biotin dissolves in N,N’-dimethylacetamide (DMAC) or dimethylsulfoxide (DMSO). DMAC and DMSO must be dried over 3Å molecular sieves for at least 24 hours before use. Solutions of NHS-biotin should be used immediately. Storing NHS-biotin in solution is not recommended."

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 341.38; single compound
Chemical formula C₁₄H₁₉N₃O₅S
CAS 35013-72-0
Purity > 97%
Spacers
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMAC or DMSO, difficult to get into solution.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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NHS-biotin, product number QBD-10205, is a classic, non-dPEG® biotinylation reagent. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin and react s with free primary amines. It is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.

Description

NHS-biotin, product number QBD-10205, is a classic, non-dPEG® biotinylation reagent. Biotin (5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid, CAS number 58-85-5) is a water-soluble vitamin, though it is not especially soluble in water and is used in a wide range of biotechnology and clinical applications. NHS-biotin (2,5-dioxopyrrolidin-1-yl 5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate, CAS number 35013-72-0) is the N-hydroxysuccinimide ester of biotin. The NHS ester is reactive with free primary amines. It is useful for labeling proteins, peptides, surfaces (such as silica, glass, or magnetic particles) coated with primary amines, and the like. It can also be used with amine-terminated dPEG® products to create novel biotinylated dPEG® products.

NHS-biotin, QBD-10205, is insoluble in water, but the NHS ester hydrolyzes if the product gets wet. The optimal storage temperature of NHS-biotin is -20°C. When removed from storage, NHS-biotin should be allowed to equilibrate fully to ambient temperature before opening the bottle. The product should always be handled to minimize exposure to moisture. NHS-biotin dissolves in N,N’-dimethylacetamide (DMAC) or dimethylsulfoxide (DMSO). DMAC and DMSO must be dried over 3Å molecular sieves for at least 24 hours before use. Solutions of NHS-biotin should be used immediately. Storing NHS-biotin in solution is not recommended.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight341.38; single compound
Chemical formulaC₁₄H₁₉N₃O₅S
CAS35013-72-0
Purity> 97%
Spacers 
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)DMAC or DMSO, difficult to get into solution.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
  2. Human serum albumin and HER2-binding affibody fusion proteins for targeted delivery of fatty acid-modified molecules and therapy. Daoyuan Dong, Guanjun Xia, Zhijun Li, and Zhiyu Li. Molecular Pharmaceutics. 2016, August 21, 2016. DOI: 10.1021/acs.molpharmaceut.6b00265.

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]]> PC Biotin-NHS ester https://staging.vectorlabs.com/products/pc-biotin-nhs-ester/ Tue, 19 Sep 2023 19:28:49 +0000 https://staging.vectorlabs.com/?post_type=product&p=21983 Description

PC Biotin-NHS Ester as a unique amine reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin beads. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

Specifications

Unit Size 4 x 1 mg, 10 mg, 25 mg, 100 mg
Molecular weight 840.89
Chemical composition C36H52N6O15S
CAS N/A
Solubility DMSO, DMF, DCM, THF, Chloroform
Appearance Slightly yellow amorphos solid to oil
Storage Conditions -20°C. Desiccate
Shipping Conditions Frozen

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Description

PC Biotin-NHS Ester as a unique amine reactive, photocleavable biotin reagent that allows for reagent-free release of the captured biomolecules from streptavidin beads. This reagent contains a biotin moiety linked to the amine reactive N-hydroxysuccinimidyl (NHS) mixed carbonate through a spacer arm containing a photocleavable moiety. The NHS portion of this compound reacts specifically with primary amine groups on the target molecule(s) to form a carbamate linkage. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

Specifications

Unit Size4 x 1 mg, 10 mg, 25 mg, 100 mg
Molecular weight840.89
Chemical compositionC36H52N6O15S
CASN/A
SolubilityDMSO, DMF, DCM, THF, Chloroform
AppearanceSlightly yellow amorphos solid to oil
Storage Conditions-20°C. Desiccate
Shipping ConditionsFrozen

Documents

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]]> Biotin-NHS Ester https://staging.vectorlabs.com/products/biotin-nhs-ester/ Tue, 19 Sep 2023 19:28:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=21973 Description

Biotin-NHS Ester is an amine-reactive building block used for modification of amine-containing molecule in organic media. NHS-activated biotin is water-insoluble reagent, thus requires an organic solvent such as DMSO or DMF to be dissolved. Short spacer arm adds minimal mass to modified molecules.

Specifications

Unit Size 100 mg, 1000 mg
Molecular weight 341.38
Chemical composition C14H19N3O5S
CAS N/A
Solubility DMSO, DMF
Purity >98%
Appearance White crystalline
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

Biotin-NHS Ester is an amine-reactive building block used for modification of amine-containing molecule in organic media. NHS-activated biotin is water-insoluble reagent, thus requires an organic solvent such as DMSO or DMF to be dissolved. Short spacer arm adds minimal mass to modified molecules.

Specifications

Unit Size100 mg, 1000 mg
Molecular weight341.38
Chemical compositionC14H19N3O5S
CASN/A
SolubilityDMSO, DMF
Purity>98%
AppearanceWhite crystalline
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

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