Category: Avidin & Biotinylated Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:01:55 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Category: Avidin & Biotinylated Reagents – VectorLabs https://staging.vectorlabs.com 32 32 Streptavidin, CY5 https://staging.vectorlabs.com/products/cy5-streptavidin/ Thu, 04 May 2023 04:12:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=15026 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 647 nm
Maximum Emission 663 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy5
Color of Fluorescence Far Red
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation647 nm
Maximum Emission663 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy5
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

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]]> Streptavidin, CY3 https://staging.vectorlabs.com/products/cy3-streptavidin/ Tue, 02 May 2023 19:24:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=14518 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 550 nm
Maximum Emission 570 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy3
Color of Fluorescence Red
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation550 nm
Maximum Emission570 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy3
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

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]]> Avidin D https://staging.vectorlabs.com/products/avidin-d/ Tue, 02 May 2023 02:33:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=13937 Description

Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous, ultrapure avidin with very low nonspecific binding characteristics. Avidin D is recommended for use in solid-phase assays and many histochemical applications when the technique of sequential addition of reagents (“sandwich” technique) is employed.

Specifications

Unit Size 10 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Elispot, ELISAs
Recommended Usage Dissolve in 0.1 M sodium bicarbonate. The recommended concentration range for use is 5-50 µg/ml.
Recommended Storage 2-8 °C
Molecular Weight 68000
Conjugate Unconjugated
Detection Target Biotin

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Description

Avidin D is isolated from egg white by a specific affinity system which results in a homogeneous, ultrapure avidin with very low nonspecific binding characteristics. Avidin D is recommended for use in solid-phase assays and many histochemical applications when the technique of sequential addition of reagents (“sandwich” technique) is employed.

Specifications

Unit Size10 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Elispot, ELISAs
Recommended UsageDissolve in 0.1 M sodium bicarbonate. The recommended concentration range for use is 5-50 µg/ml.
Recommended Storage2-8 °C
Molecular Weight68000
ConjugateUnconjugated
Detection TargetBiotin

Documents

Citations

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]]> Streptavidin, DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-streptavidin/ Mon, 01 May 2023 19:42:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=13589 Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 592 nm
Maximum Emission 617 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 594
Color of Fluorescence Red
Format Concentrate

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Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation592 nm
Maximum Emission617 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 594
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

]]> Streptavidin, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-streptavidin/ Mon, 01 May 2023 19:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13562 Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation493 nm
Maximum Emission518 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 488
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight® 488 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-streptavidin/ Sun, 30 Apr 2023 03:48:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=13465 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 556 nm
Maximum Emission 571 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 549
Color of Fluorescence Orange
Format Concentrate

The post Streptavidin, DyLight™ 549 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation556 nm
Maximum Emission571 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 549
Color of FluorescenceOrange
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 549 appeared first on VectorLabs.

]]> Streptavidin, Peroxidase, R.T.U. https://staging.vectorlabs.com/products/streptavidin-horseradish-peroxidase-ready-to-use/ Fri, 28 Apr 2023 21:28:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=13073 Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage For most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 µg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Ready-to-Use

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

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Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageFor most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 µg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatReady-to-Use

Documents

Citations

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 649 https://staging.vectorlabs.com/products/dylight-649-streptavidin/ Fri, 28 Apr 2023 21:23:56 +0000 https://staging.vectorlabs.com/?post_type=product&p=13063 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Excitation at 655 nm
  • Emission at 670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 655 nm
Maximum Emission 670 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 649
Color of Fluorescence Far Red
Format Concentrate

The post Streptavidin, DyLight™ 649 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Excitation at 655 nm
  • Emission at 670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation655 nm
Maximum Emission670 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 649
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

*Additional information about the images can be found here.

DyLightspectra jpg

Dylight Table1 jpg

The post Streptavidin, DyLight™ 649 appeared first on VectorLabs.

]]> Streptavidin, Alkaline Phosphatase https://staging.vectorlabs.com/products/streptavidin-alkaline-phosphatase-conjugated/ Fri, 28 Apr 2023 21:20:07 +0000 https://staging.vectorlabs.com/?post_type=product&p=13056 Description

Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
  • Specific covalent linkages are chosen to provide stable, highly active conjugates

Specifications

Unit Size 1 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage The recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage 2-8 °C
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Alkaline Phosphatase
Format Concentrate

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Description

Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
  • Specific covalent linkages are chosen to provide stable, highly active conjugates

Specifications

Unit Size1 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageThe recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage2-8 °C
Detection Enzyme(s)Alkaline Phosphatase
ConjugateAlkaline Phosphatase
FormatConcentrate

Documents

Citations

The post Streptavidin, Alkaline Phosphatase appeared first on VectorLabs.

]]> Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) https://staging.vectorlabs.com/products/streptavidin-hrp-concentrate-elisas/ Fri, 28 Apr 2023 21:15:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=13047 Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size 1 mg
Applications Blotting Applications, Elispot, ELISAs
Recommended Usage Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 mg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

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Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase

  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size1 mg
ApplicationsBlotting Applications, Elispot, ELISAs
Recommended UsageDo not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

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Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

This Horseradish peroxidase streptavidin preparation (HRP-Sav; SA-5014) is optimized for use in solid-phase assays such as ELISAs and blotting applications.

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

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