Category: Antibodies – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:13 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Category: Antibodies – VectorLabs https://staging.vectorlabs.com 32 32 VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit (red), DyLight® 488 Anti-Mouse (green) https://staging.vectorlabs.com/products/vectafluor-duet-kit-mouse-gr-rabbit-red/ Tue, 02 May 2023 19:41:06 +0000 https://staging.vectorlabs.com/?post_type=product&p=14539 Description

VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step.  Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.

Features:

  • Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
  • Affinity-purified, ultrapure, high affinity antibodies

Specifications

Unit Size 1 kit
Applications Immunofluorescence
Target Species Universal (Mouse/Rabbit)
Conjugate DyLight 488/DyLight 594
Color of Fluorescence Kit (Green, Red)
Host Species Horse
Format Ready-to-Use

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse

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Description

VectaFluor Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step.  Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor Duet is compatible with fluorescence staining of cells and tissues.

Features:

  • Robust cocktail formulation of DyLight anti-mouse IgG/anti-rabbit IgG secondary antibodies
  • Affinity-purified, ultrapure, high affinity antibodies

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence
Target SpeciesUniversal (Mouse/Rabbit)
ConjugateDyLight 488/DyLight 594
Color of FluorescenceKit (Green, Red)
Host SpeciesHorse
FormatReady-to-Use

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse

Documents

Technical Information

Kit Contents:

  • 15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking 
  • 15 ml VectaFluor Duet Reagent [DyLight 594 Anti-Rabbit IgG and DyLight 488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse, ready-to-use in 5% NHS. 

DyLight™ 488:

  • Excitation maximum:   493 nm
  • Emission maximum:  518 nm
  • Color:  Green

DyLight™ 594:

  • Excitation maximum:   592 nm
  • Emission maximum:  617 nm
  • Color:  Red

DyLight fluorescent dyes are direct alternatives to traditional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets.  

DyLight dyes offer a number of potential advantages including greater photostability and brighter fluorescence.  DyLight dyes are completely stable over a pH range of 4-9 making them compatible with many aqueous-based buffers and diluents. The DyLight dyes can be applied as single labels or in combination with other DyLight dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology.  The Dylight dyes currently offered are DyLight 488 (green), DyLight 549 (orange), DyLight 594 (red), and DyLight 649 (far red).

The post VectaFluor™ Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit (red), DyLight® 488 Anti-Mouse (green) appeared first on VectorLabs.

]]> Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-mouse-igg/ Tue, 02 May 2023 17:02:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=14446 Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Mouse
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Product FAQs

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.
 

Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Goat Anti-Mouse IgG (H+L) is supplied in solution. With some exceptions the recommended dilution for most applications is 1:200.

(H+L) indicates the antibody recognizes both heavy and light chains.

The post Goat Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-rabbit-igg/ Tue, 02 May 2023 16:58:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=14439 Description

Ready-to-Use (R.T.U.) Biotinylated Goat Anti-Rabbit IgG Secondary Antibody is a stabilized, prediluted, ready-to-use solution of our biotinylated secondary antibodies. It is used in an avidin-biotin or streptavidin-biotin detection system.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Included in the VECTASTAIN® ABC kits

Specifications

 
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Rabbit
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

Ready-to-Use (R.T.U.) Biotinylated Goat Anti-Rabbit IgG Secondary Antibody is a stabilized, prediluted, ready-to-use solution of our biotinylated secondary antibodies. It is used in an avidin-biotin or streptavidin-biotin detection system.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Included in the VECTASTAIN® ABC kits

Specifications

 
Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

The post Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/biotinylated-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 16:55:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=14432 Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits.
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

 

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target Species Universal (Mouse/Rabbit)
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

This R.T.U biotinylated universal secondary antibody binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits. 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution
  • This pan-specific, biotinylated “universal” secondary antibody is specially formulated to recognize IgG (H+L) primary antibodies from mouse and rabbit

Note: This antibody has significant cross-reactivity for rodent IgG and is not recommended for use in staining rodent tissue.
Note: This product is the same biotinylated secondary antibody included in the VECTASTAIN Elite® ABC Kit (Universal; PK-6200) and VECTASTAIN ABC-AP Kit (Universal; AK-5200), but is not the same as that provided in the VECTASTAIN Universal Quick Kit, PK-8800.

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse/anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse/Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin or streptavidin based detection reagent such as RTU VECTASTAIN®® ABC Reagent(PK-7100). * 10mM phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesUniversal (Mouse/Rabbit)
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

This biotinylated Universal secondary antibody is produced in horse and binds equally well to both mouse IgG (H+L) and rabbit IgG (H+L) primary antibodies. The high staining quality and sensitivity achieved using this product is equivalent to that obtained with either our biotinylated anti-mouse IgG (H+L) or biotinylated anti-rabbit IgG (H+L) used individually. The convenience of using a single biotinylated secondary antibody for both species of primary and the elimination of possible mistakes in using the wrong secondary antibody is an advantage of this product.

 
Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The post Horse Anti-Mouse/Rabbit IgG Antibody (H+L) (Universal), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-goat-anti-rat-igg/ Tue, 02 May 2023 16:52:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14424 Description

R.T.U. Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.  (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L)
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated goat anti-rat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rat IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Rat
Conjugate Biotinylated
Host Species Goat
Format Ready-to-Use

The post Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

R.T.U. Biotinylated Goat Anti-Rat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.  (For mouse tissue applications, please see anti-rat, mouse adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L) 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated goat anti-rat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rat IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN®Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesRat
ConjugateBiotinylated
Host SpeciesGoat
FormatReady-to-Use

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 
These antibody solutions are provided in convenient dropper bottle format for ease of use.

The post Goat Anti-Rat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-goat-igg/ Tue, 02 May 2023 16:50:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=14414 Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L)

Specifications

More Information
Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Goat
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

The Ready-to-Use (R.T.U.) Biotinylated Horse Anti-Goat IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Suitable for use with goat, sheep, and bovine IgG primary antibodies
  • Recognizes both heavy and light chains (H+L) 

Specifications

More Information
Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-goat IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Goat IgG and incubate for 30 minutes at room temperature.Wash inbuffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® Elite® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesGoat
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays and can be used for tissue and cell staining, ELISAs, and blots.

Ready-to-Use (R.T.U.) Biotinylated Secondary Antibodies are stabilized, prediluted, ready-to-use solutions of our biotinylated secondary antibodies. These antibody solutions are provided in convenient dropper bottle format for ease of use.

The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker® solution (Cat. No. SP-5030) is recommended.

The post Horse Anti-Goat IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-mouse-igg/ Tue, 02 May 2023 16:44:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=14403 Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.)
  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Can be used for tissue and cell staining, ELISAs, and blots
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target Species Mouse
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

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Description

R.T.U. Biotinylated Horse Anti-Mouse IgG Antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Affinity-purified, ultrapure, high affinity antibody 
  • Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species. (For rat tissue applications, please see anti-mouse, rat adsorbed, secondary antibodies.) 
  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Can be used for tissue and cell staining, ELISAs, and blots 
  • Included in the VECTASTAIN® ABC kits
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-mouse IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Mouse IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100). * 10mM sodium phosphate, pH 7.8, 0.15M NaCl.
Target SpeciesMouse
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

Documents

Product FAQs

Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.

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Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. These antibodies are subjected to rigorous quality control assays.

The post Horse Anti-Mouse IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. https://staging.vectorlabs.com/products/rtu-biotinylated-horse-anti-rabbit-igg/ Tue, 02 May 2023 16:41:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=14396 Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.

Features:

  • Recognizes both heavy and light chains (H+L)
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
  • Supplied in a convenient ready-to-use prediluted, stabilized solution

Specifications

Unit Size 50 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration Ready-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage 2-8 °C
Recommended Usage Following incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target Species Rabbit
Conjugate Biotinylated
Host Species Horse
Format Ready-to-Use

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Description

The R.T.U. biotinylated horse anti-rabbit IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots. 

Features:

  • Recognizes both heavy and light chains (H+L) 
  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 
  • Supplied in a convenient ready-to-use prediluted, stabilized solution 

Specifications

Unit Size50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
ConcentrationReady-to-use solution of biotinylated horse anti-rabbit IgG in PBS* containing carrier protein and 0.08% sodium azide as preservative.
Recommended Storage2-8 °C
Recommended UsageFollowing incubation with primary antibody, wash specimens in buffer.Apply RTU Biotinylated Anti-Rabbit IgG and incubate for 30 minutes at room temperature.Wash in buffer for 5 minutes and follow with avidin- or streptavidin-based detection reagent such as RTU VECTASTAIN® ABC Reagent (PK-7100).
Target SpeciesRabbit
ConjugateBiotinylated
Host SpeciesHorse
FormatReady-to-Use

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Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. 

These antibody solutions are provided in convenient dropper bottle format for ease of use.

Biotinylated Horse Anti-Rabbit IgG (H+L) Antibody can be used in single antigen labeling or paired with our biotinylated horse anti-mouse IgG in multiple antigen labeling applications.

This antibody can be detected with the VECTASTAIN® ABC systems or other (strept)avidin reagent

The post Horse Anti-Rabbit IgG Antibody (H+L), Biotinylated, R.T.U. appeared first on VectorLabs.

]]> Anti-Streptavidin Antibody, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-anti-streptavidin/ Tue, 02 May 2023 02:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13944 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Streptavidin
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetStreptavidin
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

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Citations

Technical Information

The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

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]]> Goat Anti-Human Kappa Chain Antibody, Biotinylated https://staging.vectorlabs.com/products/biotinylated-goat-anti-human-kappa-chain/ Tue, 02 May 2023 02:29:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=13929 Description

Biotinylated Goat Anti-Human Kappa Chain Antibody is a chain-specific antibody that can be used for tissue and cell staining, ELISAs, and blots. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Features:

  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody

Specifications

Unit Size 0.5 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage 2–8 °C
Recommended Usage Reconstitute by adding 1.0 ml water. The resulting solution will have the following composition: 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species Human
Conjugate Biotinylated
Host Species Goat
Format Concentrate

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Description

Biotinylated Goat Anti-Human Kappa Chain Antibody is a chain-specific antibody that can be used for tissue and cell staining, ELISAs, and blots. This kappa chain specific antibody has virtually no cross-reactivity with other immunoglobulin classes or other heavy or light chains.

Features:

  • Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody 

Specifications

Unit Size0.5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage2–8 °C
Recommended UsageReconstitute by adding 1.0 ml water. The resulting solution will have the following composition: 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target SpeciesHuman
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. 

Biotinylated Goat Anti-Human Kappa Chain is supplied in solution form. With some exceptions the recommended dilution for most applications is 1:100.

This chain-specific antibody is produced specifically to distinguish between chains or classes of target immunoglobulins. This antibody is affinity-purified and treated by negative solid-phase adsorption to remove cross-reacting antibodies. This biotinylated conjugate can be employed in flow cytometry or used for tissue staining.

The post Goat Anti-Human Kappa Chain Antibody, Biotinylated appeared first on VectorLabs.

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