Anterograde/Retrograde – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 16:26:48 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Anterograde/Retrograde – VectorLabs https://staging.vectorlabs.com 32 32 NEUROBIOTIN® Tracer https://staging.vectorlabs.com/products/neurobiotin-tracer/ Tue, 02 May 2023 15:56:09 +0000 https://staging.vectorlabs.com/?post_type=product&p=14294 Description

NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling.

Features:

  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size 20 mg, 50 mg
Chemical Formula C12H23ClN4O2S
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen.
Molecular Weight 322.8
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

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Description

NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling.

Features:

  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size20 mg, 50 mg
Chemical FormulaC12H23ClN4O2S
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen.
Molecular Weight322.8
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

The post NEUROBIOTIN® Tracer appeared first on VectorLabs.

]]> NEUROBIOTIN® 350 Tracer https://staging.vectorlabs.com/products/neurobiotin-350/ Tue, 02 May 2023 00:21:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=13778 Description

NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic

Specifications

Unit Size 2 mg
Maximum Emission 452 nm
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage NEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: •a biotin label with a biotinidase-resistant linker. •a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350. •a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde
Molecular Weight 573 g/mol
Maximum Excitation 346 nm
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

The post NEUROBIOTIN® 350 Tracer appeared first on VectorLabs.

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Description

NEUROBIOTIN 350 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright blue fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size2 mg
Maximum Emission452 nm
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageNEUROBIOTIN 350 is soluble at >5% in the following: water, 1M potassium acetate, 1 M KCl, 1 M NaCl, 1 M Tris, pH 8.5, and 100 mM Tris, pH 7.5.Solubility in phosphate buffers is significantly lower.Solvents and concentrations will require optimization depending on the application.It may be important to note that unbuffered NEUROBIOTIN 350 is somewhat acidic and may require buffering for some applications. NEUROBIOTIN 350 is a tri-functional molecule designed for neuronal tracing and cell filling that contains: •a biotin label with a biotinidase-resistant linker. •a bright fluorophore, similar in fluorescence to AMCA or Alexa Fluor® 350. •a fixable primary amino group. Fixable with formaldehyde or glutaraldehyde
Molecular Weight573 g/mol
Maximum Excitation346 nm
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

Similar in structure to NEUROBIOTIN 488, NEUROBIOTIN 350 is also an amine containing biotin derivative. However, it is conjugated with a bright blue fluorophore that would contrast with other green or red fluorescent markers or reagents present in the same preparation. For administration purposes NEUROBIOTIN 350 has a net positive charge and is highly soluble in a number of commonly used buffer and salt solutions.

The post NEUROBIOTIN® 350 Tracer appeared first on VectorLabs.

]]> NEUROBIOTIN®-PLUS Tracer https://staging.vectorlabs.com/products/neurobiotin-plus/ Tue, 02 May 2023 00:15:53 +0000 https://staging.vectorlabs.com/?post_type=product&p=13768 Description

NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling

Features:

  • Fixable primary amine
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size 5 mg
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage Fixable with formaldehyde or glutaraldehyde
Solution 10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide.
Molecular Weight 358 g/mol
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

The post NEUROBIOTIN®-PLUS Tracer appeared first on VectorLabs.

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Description

NEUROBIOTIN-Plus Tracer is a biotin label that is impervious to cleavage and breakdown by biotinidase. It is used for visualizing neural architecture and for the identification of gap junction coupling

Features:

  • Fixable primary amine
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading
  • Biotin label can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size5 mg
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageFixable with formaldehyde or glutaraldehyde
Solution10% solutions can be made in water, buffers, 1 M KCl, 1 M NaCl, or dimethylsulfoxide.
Molecular Weight358 g/mol
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

Investigators have identified rapid degradation of injected tracers such as biocytin due to the presence of biotinidase activity in brain tissue*. This loss of stability of the tracer significantly reduces the viable postinjection period and may compromise complete detection of a neural network. NEUROBIOTIN-Plus was designed to be impervious to cleavage and breakdown by biotinidase. This newly synthesized biotin-containing compound contains fixable amine groups, is highly soluble and has a low molecular weight. These characteristics make it an ideal candidate for experiments requiring long postinjection survival times and optimal uptake along the entire neural tract.

NEUROBIOTIN-Plus shares the same key advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde/li>

NEUROBIOTIN-Plus can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods.

*Mishra, A. et al (2010) ACS Chem. Neurosci. 1:129-138.

The post NEUROBIOTIN®-PLUS Tracer appeared first on VectorLabs.

]]> NEUROBIOTIN® 488 Tracer https://staging.vectorlabs.com/products/neurobiotin-488/ Tue, 02 May 2023 00:08:57 +0000 https://staging.vectorlabs.com/?post_type=product&p=13758 Description

NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic

Specifications

Unit Size 2 mg
Maximum Emission 517 nm
Recommended Storage 2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended Usage NEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight 747 g/mol
Maximum Excitation 493 nm
Neuronal Tracer – Direction of Transport Anterograde/Retrograde
Detection Method Avidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

The post NEUROBIOTIN® 488 Tracer appeared first on VectorLabs.

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Description

NEUROBIOTIN 488 Tracer is a tri-functional molecule designed for neuronal tracing and cell filling.

Features:

  • Bright green fluorophore, similar in fluorescence to fluorescein, Cy2 or Alexa Fluor® 488
  • Biotin label with a biotinidase-resistant linkage
  • Fixable primary amine
  • Used for visualizing neural architecture and for the identification of gap junction coupling
  • Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells
  • Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading

 

Advantages of NEUROBIOTIN Tracer over biocytin and other neuronal labels:

  • Better solubility
  • More efficiently iontophoresed
  • Remains in cell longer
  • Non-toxic
  • Can be fixed with formalin or glutaraldehyde

Specifications

Unit Size2 mg
Maximum Emission517 nm
Recommended Storage2-8 °C (desiccated). Once in solution, store frozen. This product does not contain an antimicrobial agent.
Recommended UsageNEUROBIOTIN 488 is soluble under the following conditions.Solvents and concentrations will require optimization depending on the application. Up to 10% in 3.0 M Tris, pH 8.8 Up to 2% in 0.25 M Tris, pH 8.8 or 0.25 M Tris, 1.0 M NaCl, pH 8.8 Up to 2% in 0.1 M bicarbonate buffer, pH 8.5 or 0.25 M bicarbonate, 1.0 M NaCl, pH 8.5 Up to 0.5% in 0.13 M cesium gluconate, 10 mM HEPES, pH 7.2 Up to 10% in DMSO
Molecular Weight747 g/mol
Maximum Excitation493 nm
Neuronal Tracer – Direction of TransportAnterograde/Retrograde
Detection MethodAvidin(Streptavidin)/Biotin Method, Chromogenic, Fluorescence

Documents

Citations

Technical Information

NEUROBIOTIN 488 Tracer is transported along axons and fine projections and is compatible with patch clamp applications and pressure injection methods. NEUROBIOTIN 488 Tracer is readily fixed with standard aldehyde based fixatives. This tracer can be viewed directly under fluorescence microscopy. 

The post NEUROBIOTIN® 488 Tracer appeared first on VectorLabs.

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