Quenchers – VectorLabs

SY-7 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:22 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-7 NHS Ester (QSY®-7 succinimidyl ester equivalent) is a nonfluorescent acceptor dye for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 520 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amines
Abs/Em Maxima	560 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight	791.32
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-7 NHS ester appeared first on VectorLabs.

SY-7 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:21 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-7 DBCO (QSY®-7 equivalent) is a nonfluorescent acceptor dye for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 520 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes.

SY-7 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern SY-7 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Azide
Abs/Em Maxima	500 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight	1067.24
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-7 DBCO appeared first on VectorLabs.

UBQ-2 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:19 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

Universal Black 2 Quencher (UBQ-2, Black Hole Quencher®-2 (BHQ®-2) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 560-670 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes such as Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, and IRDye® 650.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	580 nm
Extinction coefficient	38,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650
Molecular weight	603.59
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post UBQ-2 NHS Ester appeared first on VectorLabs.

UBQ-1 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:18 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	534 nm
Extinction coefficient	34,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488
Molecular weight	586.58
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post UBQ-1 NHS Ester appeared first on VectorLabs.

UBQ-3 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:16 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	615 nm
Extinction coefficient	40,700 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Cy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight	664.76
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post UBQ-3 NHS Ester appeared first on VectorLabs.

UBQ-3 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:14 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

UBQ-3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice for labeling intact proteins, lipids and peptides where the presence of cytotoxic copper catalyst is not acceptable.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	615 nm
Extinction coefficient	40,700 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Cy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight	958.15
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

The post UBQ-3 DBCO appeared first on VectorLabs.

SY-21 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:12 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-21NHS Ester (QSY®-21 succinimidyl ester equivalent) is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	661 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5.
Molecular weight	815.34
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-21 NHS ester appeared first on VectorLabs.

SY-21 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:18:52 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-21 DBCO (QSY®-21 equivalent) is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.

SY-21 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern SY-21 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Azide
Abs/Em Maxima	661 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5.
Molecular weight	1091.27
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-21 DBCO appeared first on VectorLabs.

SY-9 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:18:50 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-9 (QSY®-9 equivalent) is a more hydrophilic version of the popular SY-7 dark quencher (QSY®-7 equivalent). This nonfluorescent acceptor dye is often used for the preparation of peptide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with dyes emitting at 500 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	560 nm
Extinction coefficient	85,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight	914.97
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-9 NHS ester appeared first on VectorLabs.

SY-9 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:18:49 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-9 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	560 nm
Extinction coefficient	85,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX
Molecular weight	1227.36
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-9 DBCO appeared first on VectorLabs.