800 nm – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:20:27 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 800 nm – VectorLabs https://staging.vectorlabs.com 32 32 AQuora® 800-Maleimide https://staging.vectorlabs.com/products/aquora-800-maleimide/ Sat, 24 Feb 2024 02:14:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=37471 AQuora® 800-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® 800-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 800-Maleimide is a cyanine-based dye with photophysical properties comparable to Alexa Fluor® 790, DyLight® 800, Dylight® 800 4x PEG, and IRDye® 800 dye.

This AQuora® 800-Maleimide dye is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific. IRDye® is a registered trademark of Licor®.

Specifications

Unit Size 1mg
Format Solid
Storage Store at -20°C. Protect from light. May ship at ambient temperatures.
Country of Origin United States of America
Label AQuora® 800
Excitation / Emission Maximum 792 nm / 809 nm
A280 Correction Factor 0.045
Reactive Group Maleimide
Extinction Coefficient 270,000
Spectrally Similar Dyes Alexa Fluor® 790 , DyLight® 800, Dylight® 800 4x PEG, and IRDye® 800 dye
Molecular Weight 2030

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Description

AQuora® 800-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® 800-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 800-Maleimide is a cyanine-based dye with photophysical properties comparable to Alexa Fluor® 790, DyLight® 800, Dylight® 800 4x PEG, and IRDye® 800 dye.

This AQuora® 800-Maleimide dye is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond.
Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific. IRDye® is a registered trademark of Licor®.

Specifications

Unit Size1mg
FormatSolid
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® 800
Excitation / Emission Maximum792 nm / 809 nm
A280 Correction Factor0.045
Reactive GroupMaleimide
Extinction Coefficient270,000
Spectrally Similar DyesAlexa Fluor® 790 , DyLight® 800, Dylight® 800 4x PEG, and IRDye® 800 dye
Molecular Weight2030

Documents

The post AQuora® 800-Maleimide appeared first on VectorLabs.

]]> MB 800Z NHS ester https://staging.vectorlabs.com/products/mb-800z-nhs-ester/ Tue, 19 Sep 2023 22:32:10 +0000 https://staging.vectorlabs.com/?post_type=product&p=23072 Description

laser lineMB 800Z (also known as s775z) is a conceptually new class of sterically shielded NIR cyanine heptamethine dye that was developed at professor Bradley D. Smith laboratory. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface (see description section).

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces. MB 800Z will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.12,13

[caption id="attachment_28227" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Primary amine
Abs/Em Maxima 774/798 nm
Extinction coefficient 205,000 cm-1M-1
Solubility Water, DMSO, DMF
Spectrally similar dyes IRDye® 800, DyLight® 800
Molecular weight 1471.67 (protonated)
Storage Conditions -50°C to -85°C
Shipping Conditions Dry ice

The post MB 800Z NHS ester appeared first on VectorLabs.

]]>

Description

laser lineMB 800Z (also known as s775z) is a conceptually new class of sterically shielded NIR cyanine heptamethine dye that was developed at professor Bradley D. Smith laboratory. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface (see description section).

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces. MB 800Z will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.12,13

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima774/798 nm
Extinction coefficient205,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesIRDye® 800, DyLight® 800
Molecular weight1471.67 (protonated)
Storage Conditions-50°C to -85°C
Shipping ConditionsDry ice

Abs/Em Spectra

MB800 1

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Selected References

  1. Van Leeuwen, F. W. B., et al. (2016). Synthesis and systematic evaluation of symmetric sulfonated centrally Csingle bondC bonded cyanine near-infrared dyes for protein labelling. Elsevier, Dyes and Pigments, 132, 7-19. [ScienceDirect]
  2. Wheat, T. E., et al. (2002). IRDye78 conjugates for near-infrared fluorescence imaging Mol Imaging, 1 (4), 354-64. [PubMed]
  3. Clarke, D. T., et al. (2012). Multicolour Single Molecule Imaging in Cells with Near Infra-Red Dyes. PNAS, No. e36265. [PLoS One]
  4. Robinson, C. M., et al. (2019). A Nonaggregating Heptamethine Cyanine for Building Brighter Labeled Biomolecules. ACS Chem. Biol, 14, 934−940. [ACS Publications]
  5. Gibbs, S., et al. (2013). Targeted zwitterionic near-infrared fluorophores for improved optical imaging. Nat Biotechnol, 31, 148-153. [Nature Biotechnology]
  6. Hartman, Y., et al. (2015). Fluorescence-guided resection of experimental malignant glioma using cetuximab-IRDye 800CW. Br J Neurosurg., 29, 850–858. [PubMed]
  7. Vereb, G., et al. (2018). The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes. Biophys. J., 114, 688-700. [PubMed]
  8. Harms, G. S., et al. (2000). Anomalous Fluorescence Enhancement of Cy3 and Cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to IgG and Noncovalent Binding to Avidin. Bioconjugate Chem., 11, 696−704. [ACS Publications]
  9. Hamann, F. M., et al. (2011). Suitable Labels for Molecular Imaging – Influence of Dye Structure and Hydrophilicity on the Spectroscopic Properties of IgG Conjugates. Bioconjugate Chem., 22 (7), 1298–1308. [ACS Publications]
  10. Smith, B. D, et al. (2020). Sterically Shielded Heptamethine Cyanine Dyes for Bioconjugation and High Performance Near‐Infrared Fluorescence Imaging. Angew. Chem. Int. Ed., 59, 12154–12161. [PubMed]
  11. Smith, B. D, et al. (2021). High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Analytical Chemistry, 93 (7), 3643-3651. [PubMed]
  12. Schnermann, M. J., et al. (2016). Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates . Bioconjugate Chem., 27 (2), 404-13. [PubMed]
  13. Ramsey, J. D., et al. (2020). Interactions between Biomolecules and Zwitterionic Moieties: A Review. Biomacromolecules, 21 (7), 2557-2573. [PubMed]

Documents

The post MB 800Z NHS ester appeared first on VectorLabs.

]]> AZDye 800 Picolyl Azide https://staging.vectorlabs.com/products/azdye-800-picolyl-azide/ Tue, 19 Sep 2023 20:18:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=22641 Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

[caption id="attachment_27084" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 775/799 nm
Extinction Coefficient
204,000
Spectrally Similar Dyes IRDye® 800CW, CF® 800, DyLight® 800
Molecular weight 1621.83 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Green solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 800 Picolyl Azide appeared first on VectorLabs.

]]>

AZDye™ 800 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

AZDye™ 800 is a conceptually new class of sterically shielded NIR cyanine heptamethine dye. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. Its excitation ideally suited for the 800nm channel of imaging systems. AZDye™ 800 is spectrally is almost identical to IRDye 800CW and DyLight 800.

Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima775/799 nm
Extinction Coefficient
204,000
Spectrally Similar DyesIRDye® 800CW, CF® 800, DyLight® 800
Molecular weight1621.83 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceGreen solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB800

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  4. Van Leeuwen, F. W. B., et al. (2016). Synthesis and systematic evaluation of symmetric sulfonated centrally Csingle bondC bonded cyanine near-infrared dyes for protein labelling. Elsevier, Dyes and Pigments, 132, 7-19. [ScienceDirect]
  5. Wheat, T. E., et al. (2002). IRDye78 conjugates for near-infrared fluorescence imaging Mol Imaging, 1 (4), 354-64. [PubMed]
  6. Clarke, D. T., et al. (2012). Multicolour Single Molecule Imaging in Cells with Near Infra-Red Dyes. PNAS, No. e36265. [PLoS One]
  7. Robinson, C. M., et al. (2019). A Nonaggregating Heptamethine Cyanine for Building Brighter Labeled Biomolecules. ACS Chem. Biol, 14, 934−940. [ACS Publications]
  8. Gibbs, S., et al. (2013). Targeted zwitterionic near-infrared fluorophores for improved optical imaging. Nat Biotechnol, 31, 148-153. [Nature Biotechnology]
  9. Hartman, Y., et al. (2015). Fluorescence-guided resection of experimental malignant glioma using cetuximab-IRDye 800CW. Br J Neurosurg., 29, 850–858. [PubMed]
  10. Vereb, G., et al. (2018). The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes. Biophys. J., 114, 688-700. [PubMed]
  11. Harms, G. S., et al. (2000). Anomalous Fluorescence Enhancement of Cy3 and Cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to IgG and Noncovalent Binding to Avidin. Bioconjugate Chem., 11, 696−704. [ACS Publications]
  12. Hamann, F. M., et al. (2011). Suitable Labels for Molecular Imaging – Influence of Dye Structure and Hydrophilicity on the Spectroscopic Properties of IgG Conjugates. Bioconjugate Chem., 22 (7), 1298–1308. [ACS Publications]
  13. Smith, B. D, et al. (2020). Sterically Shielded Heptamethine Cyanine Dyes for Bioconjugation and High Performance Near‐Infrared Fluorescence Imaging. Angew. Chem. Int. Ed., 59, 12154–12161. [PubMed]
  14. Smith, B. D, et al. (2021). High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Analytical Chemistry, 93 (7), 3643-3651. [PubMed]
  15. Schnermann, M. J., et al. (2016). Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates . Bioconjugate Chem., 27 (2), 404-13. [PubMed]
  16. Ramsey, J. D., et al. (2020). Interactions between Biomolecules and Zwitterionic Moieties: A Review. Biomacromolecules, 21 (7), 2557-2573. [PubMed]
  17.  

The post AZDye 800 Picolyl Azide appeared first on VectorLabs.

]]> AZDye 800 Azide https://staging.vectorlabs.com/products/azdye-800-azide/ Tue, 19 Sep 2023 20:18:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=22635 Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

[caption id="attachment_27084" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 775/799 nm
Extinction Coefficient
206,000
Spectrally Similar Dyes IRDye® 800CW, CF® 800, DyLight® 800
Molecular weight 1543.80 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Appearance Green solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 800 Azide appeared first on VectorLabs.

]]>

AZDye™ 800 is a conceptually new class of sterically shielded NIR cyanine heptamethine dye. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness.

AZDye™ 800 Azide is a water-soluble, NIR-fluorescent probe ideally suited for the 800nm channel of imaging systems. AZDye™ 800 is spectrally is almost identical to IRDye 800CW and DyLight 800.

Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima775/799 nm
Extinction Coefficient
206,000
Spectrally Similar DyesIRDye® 800CW, CF® 800, DyLight® 800
Molecular weight1543.80 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
AppearanceGreen solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB800

Documents

Selected References

  1. Van Leeuwen, F. W. B., et al. (2016). Synthesis and systematic evaluation of symmetric sulfonated centrally Csingle bondC bonded cyanine near-infrared dyes for protein labelling. Elsevier, Dyes and Pigments, 132, 7-19. [ScienceDirect]
  2. Wheat, T. E., et al. (2002). IRDye78 conjugates for near-infrared fluorescence imaging Mol Imaging, 1 (4), 354-64. [PubMed]
  3. Clarke, D. T., et al. (2012). Multicolour Single Molecule Imaging in Cells with Near Infra-Red Dyes. PNAS, No. e36265. [PLoS One]
  4. Robinson, C. M., et al. (2019). A Nonaggregating Heptamethine Cyanine for Building Brighter Labeled Biomolecules. ACS Chem. Biol, 14, 934−940. [ACS Publications]
  5. Gibbs, S., et al. (2013). Targeted zwitterionic near-infrared fluorophores for improved optical imaging. Nat Biotechnol, 31, 148-153. [Nature Biotechnology]
  6. Hartman, Y., et al. (2015). Fluorescence-guided resection of experimental malignant glioma using cetuximab-IRDye 800CW. Br J Neurosurg., 29, 850–858. [PubMed]
  7. Vereb, G., et al. (2018). The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes. Biophys. J., 114, 688-700. [PubMed]
  8. Harms, G. S., et al. (2000). Anomalous Fluorescence Enhancement of Cy3 and Cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to IgG and Noncovalent Binding to Avidin. Bioconjugate Chem., 11, 696−704. [ACS Publications]
  9. Hamann, F. M., et al. (2011). Suitable Labels for Molecular Imaging – Influence of Dye Structure and Hydrophilicity on the Spectroscopic Properties of IgG Conjugates. Bioconjugate Chem., 22 (7), 1298–1308. [ACS Publications]
  10. Smith, B. D, et al. (2020). Sterically Shielded Heptamethine Cyanine Dyes for Bioconjugation and High Performance Near‐Infrared Fluorescence Imaging. Angew. Chem. Int. Ed., 59, 12154–12161. [PubMed]
  11. Smith, B. D, et al. (2021). High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Analytical Chemistry, 93 (7), 3643-3651. [PubMed]
  12. Schnermann, M. J., et al. (2016). Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates . Bioconjugate Chem., 27 (2), 404-13. [PubMed]
  13. Ramsey, J. D., et al. (2020). Interactions between Biomolecules and Zwitterionic Moieties: A Review. Biomacromolecules, 21 (7), 2557-2573. [PubMed]

The post AZDye 800 Azide appeared first on VectorLabs.

]]> AZDye 800 DBCO https://staging.vectorlabs.com/products/azdye-800-dbco/ Tue, 19 Sep 2023 20:18:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=22629 Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

[caption id="attachment_27084" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 775/799 nm
Extinction Coefficient
204,000
Spectrally Similar Dyes IRDye® 800CW, CF® 800, DyLight® 800
Molecular weight 1677.94
CAS N/A
Solubility Water, DMSO, DMF
Appearance Green solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post AZDye 800 DBCO appeared first on VectorLabs.

]]>

AZDye™ 800 is a conceptually new class of sterically shielded NIR cyanine heptamethine dye. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus dramatically enhances the fluorescence brightness. Its excitation ideally suited for the 800nm channel of imaging systems. AZDye™ 800 is spectrally is almost identical to IRDye 800CW and DyLight 800.

AZDye™ 800 DBCO is a water-soluble, NIR-fluorescent probe for copper-less detection of azide-tagged biomolecules. In application where the presence of copper is a concern AZDye™ 800 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Description

Cyanine heptamethine dyes are well-known NIR fluorophores, with emission wavelengths >740 nm, and often are reagents of choice for labeling antibodies. Two of the most popular commercial NIR cyanine heptamethine dyes for antibody conjugation are IRDye 800CW and DyLight 800. While these NIR dyes are undoubtedly useful for many types of immunofluorescence technologies, the resulting NIR dye-labeled antibodies sometimes exhibit performance limitations due to three inherent fluorophore concerns. (1) A meso-OAryl group connected directly to the heptamethine fluorochrome group is susceptible to nucleophilic displacement by biological amines and thiols1, 2 resulting in a diminished chemical stability of the dye-antibody conjugates during synthesis, storage, or the time-course of an imaging experiment. In addition, the electron-donating meso-OAryl group promotes high fluorochrome reactivity with electrophilic singlet oxygen, resulting in relatively poor dye photostability.3,4 (2) Both dyes are flat molecules with a hydrophobic core and a polyanionic charge periphery. Thus, the chemical conversion of a small polar, cationic lysine residue on the antibody surface to a large hydrophobic, polyanionic dye derivative has the potential to produce substantial changes in antibody folding and physiochemical properties, leading to lower antibody stability and decreased target specificity.4-8 (3) When activated versions of these hydrophobic dyes are conjugated to an antibody surface, they tend to attach at the proximal lysine sites as stacked face-to-face dimers, which produces a diagnostic H-dimer peak in the absorbance spectra that is nonfluorescent.8,9 Moreover, the close stacking of proximal conjugated IRDye 800CW or DyLight800 on an antibody surface amplifies the potential for a deleterious effect on antibody targeting because of a localized patch of polyanionic charge and hydrophobicity.

In order to address these drawbacks of commercially NIR cyanine a conceptually new class of sterically shielded NIR dyes was developed in Dr. Bradley D. Smith laboratory.10, 11 These dyes contain two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes and thus enhance the fluorescence brightness. A recently published antibody labeling study clearly demonstrated 1-2 order of magnitude increase in brightness compared to commercially available NIR dyes as a result of almost complete prevention of stacking of multiple fluorophores appended to the antibody surface.10, 11

These sterically shielded NIR dyes with greatly improved chemical and photochemical stability and substantially enhanced brightness will enable researchers to greatly improve various types of indirect NIR immunofluorescence imaging and diagnostics applications that require high sensitivity and also develop new photonintense techniques that require high photostability.

MB 800Z is a zwiteroinic, charge-balanced dye with an equal number of anionic sulfonate and cationic ammonium residues, a structural feature that is known to reduce interactions with off -target biological surfaces.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima775/799 nm
Extinction Coefficient
204,000
Spectrally Similar DyesIRDye® 800CW, CF® 800, DyLight® 800
Molecular weight1677.94
CASN/A
SolubilityWater, DMSO, DMF
AppearanceGreen solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB800

Documents

Selected References

  1. Van Leeuwen, F. W. B., et al. (2016). Synthesis and systematic evaluation of symmetric sulfonated centrally Csingle bondC bonded cyanine near-infrared dyes for protein labelling. Elsevier, Dyes and Pigments, 132, 7-19. [ScienceDirect]
  2. Wheat, T. E., et al. (2002). IRDye78 conjugates for near-infrared fluorescence imaging Mol Imaging, 1 (4), 354-64. [PubMed]
  3. Clarke, D. T., et al. (2012). Multicolour Single Molecule Imaging in Cells with Near Infra-Red Dyes. PNAS, No. e36265. [PLoS One]
  4. Robinson, C. M., et al. (2019). A Nonaggregating Heptamethine Cyanine for Building Brighter Labeled Biomolecules. ACS Chem. Biol, 14, 934−940. [ACS Publications]
  5. Gibbs, S., et al. (2013). Targeted zwitterionic near-infrared fluorophores for improved optical imaging. Nat Biotechnol, 31, 148-153. [Nature Biotechnology]
  6. Hartman, Y., et al. (2015). Fluorescence-guided resection of experimental malignant glioma using cetuximab-IRDye 800CW. Br J Neurosurg., 29, 850–858. [PubMed]
  7. Vereb, G., et al. (2018). The Effect of Fluorophore Conjugation on Antibody Affinity and the Photophysical Properties of Dyes. Biophys. J., 114, 688-700. [PubMed]
  8. Harms, G. S., et al. (2000). Anomalous Fluorescence Enhancement of Cy3 and Cy3.5 versus Anomalous Fluorescence Loss of Cy5 and Cy7 upon Covalent Linking to IgG and Noncovalent Binding to Avidin. Bioconjugate Chem., 11, 696−704. [ACS Publications]
  9. Hamann, F. M., et al. (2011). Suitable Labels for Molecular Imaging – Influence of Dye Structure and Hydrophilicity on the Spectroscopic Properties of IgG Conjugates. Bioconjugate Chem., 22 (7), 1298–1308. [ACS Publications]
  10. Smith, B. D, et al. (2020). Sterically Shielded Heptamethine Cyanine Dyes for Bioconjugation and High Performance Near‐Infrared Fluorescence Imaging. Angew. Chem. Int. Ed., 59, 12154–12161. [PubMed]
  11. Smith, B. D, et al. (2021). High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Analytical Chemistry, 93 (7), 3643-3651. [PubMed]
  12. Schnermann, M. J., et al. (2016). Role of Fluorophore Charge on the In Vivo Optical Imaging Properties of Near-Infrared Cyanine Dye/Monoclonal Antibody Conjugates . Bioconjugate Chem., 27 (2), 404-13. [PubMed]
  13. Ramsey, J. D., et al. (2020). Interactions between Biomolecules and Zwitterionic Moieties: A Review. Biomacromolecules, 21 (7), 2557-2573. [PubMed]

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