AZDye™ 647 Hydrazide is a bright, far-red-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AZDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.

AZDye™ 647 (Alexa Fluor ® 647 analog) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

[caption id="attachment_25905" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Cy5 Amine is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

Cy5 Amine (also sold under the Sulfo-Cyanine5 Amine name) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Cy5 Maleimide (also sold under Sulfo-Cyanine5 Maleimide) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. We do not recommended using Cy5 Maleimide for labeling proteins at high molar ration due significant self-quenching. Cy5 conjugates are only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AF 647 Maleimide (Alexa Fluor ® 647 analog) or IR 647 (IRDye® 647 analog), which can attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Spectra

Specifications

Sulfo Cy5 Maleimide is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Sulfo Cy5, AZDye 647 or IR 650 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. Sulfo Cy5 conjugates are only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 647 Maleimide or IR 647 Maleimide, which can attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Spectra

Specifications

IR 650 DBCO (IRDye® 650 DBCO analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

IR 650 DBCO is a bright, far-red-fluorescent, pH insensitive azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. IR 650 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern IR 650 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Abs/Em Spectra

Specifications

Cy5 dye (also sold under Sulfo-Cyanine5 name) is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. We do not recommend using Cy5 NHS ester for labeling proteins at high molar ration due significant self-quenching. Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 647 (Alexa Fluor® 647 analog), or IR 650 dye (IRDye® 650 analog), which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

[caption id="attachment_24122" align="alignnone" width="1200"] Abs/Em Spectra[/caption]

Specifications

Sulfo-Cy5 dye is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Sulfo-Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Sulfo-Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Sulfo-Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 647 NHS Ester analog, or IR 650 NHS Ester, which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Abs/Em Spectra

Specifications

Sulfo-Cy5 dye is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Sulfo-Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Sulfo-Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The carboxylic acid of Sulfo Cy5 dye is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. Cy5 Acid is also often used for control experiments, and for calibration.

Abs/Em Spectra

Specifications