In application where the presence of copper is a concern TAMRA DBCO is an ideal alternative to copper requiring fluorescent alkynes.

TAMRA DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaCNH₃).

Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

5-TAMRA cadaverine can used to modify carboxylic acid group in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be reversibly coupled to aldehydes and ketones to form a Schiff base – which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH₄) or sodium cyanoborohydride (NaCNH₃). Although the mixed isomers of 5(6)-TAMRA cadaverine is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

[caption id="attachment_24144" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

5(6)-TAMRA NHS Ester (5(6)-Carboxytetramethylrhodamine, TMR, TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Abs/Em Spectra

5-TAMRA NHS Ester (5-Carboxytetramethylrhodamine) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. This dye is supplied purified single isomer 5. 5-TAMRA NHS Ester has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

The NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Abs/Em Spectra

Specifications

5(6)-TAMRA Acid (5(6)-Carboxytetramethylrhodamine, TMR, TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry. The absorbance and emission maxima of TAMRA conjugates are 553 nm and 575 nm respectively.

TAMRA Acid is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. TAMRA Acid is also often used for control experiments, and for calibration.

Abs/Em Spectra

Specifications

6-TAMRA Acid (6-Carboxytetramethylrhodamine) is a single, pure isomer of carboxytetramethylrhodamine (TMR) free acid. 6-TAMRA conjugates emit bright orange-fluorescence and its excitation band ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry.

Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides, the purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids.

Abs/Em Spectra

Specifications

5-TAMRA Acid (5-Carboxytetramethylrhodamine) is a single, pure isomer of carboxytetramethylrhodamine (TMR) free acid. 5-TAMRA conjugates emit bright orange-fluorescence and its excitation band ideally suited to the 532 nm or 546 nm laser lines. It has been used widely for preparing peptide, protein, nucleotide and nucleic acid conjugates, especially fluorescent antibodies and avidin derivatives used in immunochemistry.

Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides, the purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids.

Abs/Em Spectra

Specifications