532 nm – VectorLabs

AZDye 532 Maleimide

Vector Laboratories R&D — Tue, 19 Sep 2023 23:13:08 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 532 Maleimide is a water-soluble, photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Thiol (Cys)
Abs/Em Maxima	530/555 nm
Extinction coefficient	78,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 532, DyLight® 532
Molecular weight	790.90 (protonated)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

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Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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AZDye 532 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 23:13:06 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amines
Abs/Em Maxima	530/555 nm
Extinction coefficient	81,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 532, DyLight® 532
Molecular weight	723.80
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

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Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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TAMRA Azide Plus

Vector Laboratories R&D — Tue, 19 Sep 2023 20:00:51 +0000

Description
Specifications
Abs/Em Spectra
Documents
Click Reaction Protocol

Description

Azide Plus reagents is the most recent step in improving CuAAC reaction in complex media developed by scientists at Click Chemistry Tools. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	553/575 nm
Extinction Coefficient	91,000
Flow Cytometry Laser Line	532 nm or 555 nm
Microscopy Laser Line	532 nm or 555 nm
Spectrally Similar Dyes	Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight	768.88 (protonated)
CAS	N/A
Solubility	Water, DMSO, DMF
Purity	>90% (HPLC)
Appearance	Red amorphous solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

Click Reaction Protocol for Staining Fixed/Permeabilized Cell

This is a general protocol for fixed/permeabilized cell imaging through a copper-catalyzed click reaction using the fluorescent Azide Plus reagent. We recommend using this protocol as a starting point for optimization of particular click chemistry procedures. We have found that a 1.5-3.0 μM concentration of Azide Plus reagent was optimal for most applications, including imaging of EdU incorporated into newly synthesized DNA and imaging of OPP labeled proteins without causing a high background signal. The optimal final concentration of the Azide Plus reagent is sample dependent and may range from 0.5 μM to 10 μM. Final concentrations below or above this range are also possible, and should be optimized per the specific application.

Prepare the following click solutions:
— 50 mM copper sulfate in water
— 300 mM sodium ascorbate in water (dissolve 60 mg of sodium ascorbate in 1 mL of water)
— Subtext

1 mM Azide Plus reagent in DMSO or water

Table 1

Reaction Component	Number of coverslipsor wells of a 96-well plate
Reaction Component	1 coverslip or 10 wells	5 coverslips or 50 wells	10 coverslips or 100 wells	20 coverslips or 200 wells
Reaction Buffer (Tris)	888 µL	4.4 mL	8.9 mL	17.8 mL
50 mM Copper Sulfate	10 µL	50 µL	100 µL	200 µL
AZDye Azide Plus Solution (2 µM final concentration)	2 µL	10 µL	20 µL	40 µL
Sodium ascorbate	100 µL	500 µL	1 mL	2 mL
Total Volume	1 mL	5 mL	10 mL	20 mL

Remove the permeabilization buffer (if used). Wash the cells in each well twice with 1 ml of PBS. Remove PBS.
Immediately add 1 mL of the Reaction Cocktail to the sample. Evenly distribute the reaction cocktail over the sample.
Protect from light, and incubate the plate for 30 minutes at room temperature.
6Remove the reaction cocktail. Wash each well once with 1 ml of Wash Buffer. Remove the Wash Buffer.
Wash each well with 1 mL of PBS. Remove PBS.

Click Reaction Protocol for Cell Lysates Labeling

This is a general protocol for labeling proteins in cell lysate through a copper-catalyzed click reaction using the fluorescent Azide Plus reagent. We recommend using this protocol as a starting point for optimization of particular click chemistry procedures. We have found that a 20 μM concentration of Azide Plus reagent was sufficient to label all alkyne-tagged proteins in the cell lysate without causing a high background signal. The optimal final concentration of the Azide Plus reagent is sample dependent and may range from 5 μM to 50 μM. Final concentrations below or above this range are also possible, and should be optimized per the specific application.

Prepare the following click solutions:
— 100 mM THPTA ligand in water (100 mg of THPTA in 2.3 mL of water)
— 20 mM copper sulfate in water (dissolve 11.6 mg of copper II sulfate pentahydrate in 2.3 mL of water)
— 300 mM sodium ascorbate in water (dissolve 60 mg of sodium ascorbate in 1 mL of water)
— 1 mM Azide Plus reagent in DMSO or water
For each protein lysate sample, add the following to a 1.5 mL microfuge tube, then vortex briefly to mix.
— 50 µL of protein lysate (1-5 mg/mL) in protein extraction buffer
— 120 µL of Tris buffer
— 4 µL of Azide Plus reagent stock solution (5 μM final concentration)
Add 10 µL of 100 mM THPTA solution, vortex briefly to mix.
Add 10 µL of 20 mM CuSO₄ solution, vortex briefly to mix.
Add 10 µL of 300 mM sodium ascorbate solution to initiate click reaction, vortex briefly to mix.
Vortex continuously or rotate end-over-end for 30 minutes at room temperature.
Add the labeling reaction to 3 mL of cold (–20°C) methanol, 0.75 ml of Chloroform and 2.1 mL of water. Cool it to –20°C for 1 hour.
Centrifuge for 10 minutes at 13,000-20,000 x g, then carefully remove upper aqueous layer without disturbing the interface layer containing proteins.
Add 450 µL of methanol, vortex briefly.
Centrifuge for 5 minutes at 13,000-20,000 × g to pellet protein. Carefully remove and discard supernatant.
Open the lid to microfuge tube and allow protein pellet to air dry. Do not over dry the pellet!

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AZDye 532 Azide Plus

Vector Laboratories R&D — Tue, 19 Sep 2023 19:59:21 +0000

Description
Specifications
Abs/Em Spectra
Documents
Click Reaction Protocol

Description

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	532/554 nm
Extinction Coefficient	98,000
Flow Cytometry Laser Line	532 nm
Microscopy Laser Line	532 nm
Spectrally Similar Dyes	Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight	834.88 (protonated)
CAS	N/A
Solubility	Water, DMSO, DMF
Purity	>95% (HPLC)
Appearance	Orange to light red solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

Click Reaction Protocol for Staining Fixed/Permeabilized Cell

Prepare the following click solutions:
— 50 mM copper sulfate in water
— 300 mM sodium ascorbate in water (dissolve 60 mg of sodium ascorbate in 1 mL of water)
— Subtext

1 mM Azide Plus reagent in DMSO or water

Table 1

Reaction Component	Number of coverslipsor wells of a 96-well plate
Reaction Component	1 coverslip or 10 wells	5 coverslips or 50 wells	10 coverslips or 100 wells	20 coverslips or 200 wells
Reaction Buffer (Tris)	888 µL	4.4 mL	8.9 mL	17.8 mL
50 mM Copper Sulfate	10 µL	50 µL	100 µL	200 µL
AZDye Azide Plus Solution (2 µM final concentration)	2 µL	10 µL	20 µL	40 µL
Sodium ascorbate	100 µL	500 µL	1 mL	2 mL
Total Volume	1 mL	5 mL	10 mL	20 mL

Remove the permeabilization buffer (if used). Wash the cells in each well twice with 1 ml of PBS. Remove PBS.
Immediately add 1 mL of the Reaction Cocktail to the sample. Evenly distribute the reaction cocktail over the sample.
Protect from light, and incubate the plate for 30 minutes at room temperature.
6Remove the reaction cocktail. Wash each well once with 1 ml of Wash Buffer. Remove the Wash Buffer.
Wash each well with 1 mL of PBS. Remove PBS.

Click Reaction Protocol for Cell Lysates Labeling

Prepare the following click solutions:
— 100 mM THPTA ligand in water (100 mg of THPTA in 2.3 mL of water)
— 20 mM copper sulfate in water (dissolve 11.6 mg of copper II sulfate pentahydrate in 2.3 mL of water)
— 300 mM sodium ascorbate in water (dissolve 60 mg of sodium ascorbate in 1 mL of water)
— 1 mM Azide Plus reagent in DMSO or water
For each protein lysate sample, add the following to a 1.5 mL microfuge tube, then vortex briefly to mix.
— 50 µL of protein lysate (1-5 mg/mL) in protein extraction buffer
— 120 µL of Tris buffer
— 4 µL of Azide Plus reagent stock solution (5 μM final concentration)
Add 10 µL of 100 mM THPTA solution, vortex briefly to mix.
Add 10 µL of 20 mM CuSO₄ solution, vortex briefly to mix.
Add 10 µL of 300 mM sodium ascorbate solution to initiate click reaction, vortex briefly to mix.
Vortex continuously or rotate end-over-end for 30 minutes at room temperature.
Add the labeling reaction to 3 mL of cold (–20°C) methanol, 0.75 ml of Chloroform and 2.1 mL of water. Cool it to –20°C for 1 hour.
Centrifuge for 10 minutes at 13,000-20,000 x g, then carefully remove upper aqueous layer without disturbing the interface layer containing proteins.
Add 450 µL of methanol, vortex briefly.
Centrifuge for 5 minutes at 13,000-20,000 × g to pellet protein. Carefully remove and discard supernatant.
Open the lid to microfuge tube and allow protein pellet to air dry. Do not over dry the pellet!

The post AZDye 532 Azide Plus appeared first on VectorLabs.

TAMRA Alkyne

Vector Laboratories R&D — Tue, 19 Sep 2023 19:38:02 +0000

Description
Specifications
Abs/Em Spectra
Documents
Selected References

Description

The red-fluorescent TAMRA Azide (TAMRA-PEG4-Alkyne) can be reacted with azides via a copper-catalyzed click reaction (CuAAC). TAMRA (tetramethylrhodamine) is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	553/575 nm
Extinction Coefficient	92,000
Spectrally Similar Dyes	Alexa Fluor® 546, Atto™ 543, CF® 543 Dye
Molecular weight	643.73
CAS	N/A
Solubility	DMSO, DMF
Purity	>95% (HPLC)
Appearance	Dark red amorphous solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

Selected References

Sullivan, D. K., et al. (2021). Selective Labeling and Identification of the Tumor Cell Proteome of Pancreatic Cancer In Vivo. J Proteome Res., 20 (1), 858-866. [PubMed]
Pratt Matthew R., et al. (2020). Acetylated Chemical Reporters of Glycosylation Can Display Metabolism-Dependent Background Labeling of Proteins but Are Generally Reliable Tools for the Identification of Glycoproteins. Frontiers in Chemistry, 8, 318. [Frontiers in Chemistry]
Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]

The post TAMRA Alkyne appeared first on VectorLabs.

AZDye 532 Alkyne

Vector Laboratories R&D — Tue, 19 Sep 2023 19:24:43 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye™ 532 Alkyne is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC). This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye 532 dyes are well suited to direct imaging of low-abundance targets.

The absorption/emission spectra of AZDye™ 532 is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

For application where the presence of copper is not acceptable, please consider our AZDye™ 532 DBCO probe for copper-less detection of azide-modified molecules.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	532/554 nm
Extinction Coefficient	78,000
Flow Cytometry Laser Line	532 nm
Microscopy Laser Line	532 nm
Spectrally Similar Dyes	Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight	651.61
Solubility	Water, DMSO, DMF
Appearance	Orange to light red solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

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5-TAMRA Alkyne

Vector Laboratories R&D — Tue, 19 Sep 2023 19:12:26 +0000

Description
Specifications
Abs/Em Spectra
Documents
Selected References

Description

5-TAMRA Alkyne (also known as TAMRA Alkyne, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Although the mixed isomers of 5(6)-TAMRA Alkyne is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes.

5-TAMRA Alkyne is structurally identical to Tetramethylrhodamine (TAMRA) Alkyne (5-Carboxytetramethylrhodamine, Propargylamide), 5-isomer ,Catalog number: T10183.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima	553/575 nm
Extinction Coefficient	89,000
Spectrally Similar Dyes	Alexa Fluor® 546, Atto™ 543, CF® 543 Dye
Molecular weight	467.52
CAS	N/A
Solubility	DMSO, DMF
Purity	>95% (HPLC)
Appearance	Dark red amorphous solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

Selected References

Kang, D., et al. (2021). Bioorthogonal Retro-Cope Elimination Reaction of N, N-Dialkylhydroxylamines and Strained Alkynes. J Am Chem Soc., 143 (15), 5616-5621. [PubMed]
Ranzinger, R., et al. (2020). Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation. ACS Chem. Biol., 15, 10, 2692-2701. [ACSPublications]

The post 5-TAMRA Alkyne appeared first on VectorLabs.

Dde TAMRA Biotin Alkyne

Vector Laboratories R&D — Tue, 19 Sep 2023 19:12:24 +0000

Dde TAMRA Biotin Alkyne is a trifunctional, cleavable probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a fluorescent dye (TAMRA) and biotin moiety linked to alkynee group through a spacer arm containing a hydrazine-cleavable Dde moiety. Under mild conditions (2% aqueous hydrazine), the Dde liner is cleaved, releasing the biotin tag and any avidin conjugate bound to it.

Description
Specifications
Documents

Description

Trifunctional Click Chemistry Probes that incorporate a ligation handle, a biotin and a fluorophore have become a popular tool for for tandem labeling of proteins and subsequent detection or enrichment. However, due to the strong interaction between biotin and streptavidin harsh conditions are necessary for the elution of enriched proteins. This usually leads to contamination of the sample with non-specifically bound proteins and endogenously biotinlytated proteins, which complicates target identification.

Cleavable Trifunctional Click Chemistry Probes overcome this major drawback of the streptavidin-biotin affinity purification. These probes contain a biotin moiety linked to a “clickable” group and fluorescent dye through a spacer arm containing a cleavable Dde linker. Dde moiety is stable to rigorous, denaturing wash conditions, acidic or basic conditions including generally applied buffer systems to which the biological sample may be exposed. At the same time Dde linker can be quantitatively cleaved under mild aqueous buffered conditions with 2% hydrazine.

Another very important advantage of dual label probes over regular biotin probes is built-in control. Each step of enrichment process can easily followed either by UV-Vis (550 nm) or by more sensitive fluorescence spectroscopy. After elution form streptavidin beads target proteins containing TAMRA label can be easily distinguished from non-specifically bound proteins and endogenously biotinlytated proteins.

This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Molecular weight	1439.73
Chemical composition	C71H101N12O17S
CAS	N/A
Solubility	DMSO, DMF
Appearance	Red amorphous solid
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Documents

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TAMRA Biotin Alkyne

Vector Laboratories R&D — Tue, 19 Sep 2023 19:12:22 +0000

Description
Specifications
Documents

Description

Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Azide probe can be readily incorporated into alkyne-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.

This new dual label probe should be useful to expand the new applications of standard biotin–streptavidin technology.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Molecular weight	1188.42
Chemical composition	C60H83N8O15S
CAS	N/A
Solubility	DMSO, DMF
Appearance	Red solid
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Documents

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5-TAMRA Picolyl Azide

Vector Laboratories R&D — Tue, 19 Sep 2023 17:57:23 +0000

AZDye™ 594 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). [sc name=”picolyl-azide-link”][/sc]

Description
Specifications
Abs/Em Spectra
Documents
Selected References

Description

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

5-TAMRA (also known as TAMRA, isomer 5) is the red-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	553/575 nm
Extinction Coefficient	91,000
Spectrally Similar Dyes	Alexa Fluor® 546, Atto™ 543, CF® 543 Dye
Molecular weight	808.89
CAS	N/A
Solubility	DMSO, DMF
Purity	>95% (HPLC)
Appearance	Dark red amorphous solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

Selected References

Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post 5-TAMRA Picolyl Azide appeared first on VectorLabs.