488 nm – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:50 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 488 nm – VectorLabs https://staging.vectorlabs.com 32 32 AQuora® 488-Maleimide https://staging.vectorlabs.com/products/aquora-488-maleimide/ Sat, 24 Feb 2024 02:18:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=39991 AQuora® 488-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® 488-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 488-Maleimide is a xanthene-based dye with photophysical properties comparable to Alexa Fluor 488® and DyLight® 488. It is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond. (Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.)

Specifications

Unit Size 1mg
Format Lyophilized
Storage Store at -20°C. Protect from light. May ship at ambient temperatures.
Country of Origin United States of America
Label AQuora® 488
Excitation / Emission Maximum 493 nm / 522 nm
A280 Correction Factor 0.110
Reactive Group Maleimide
Extinction Coefficient 73,000
Spectrally Similar Dyes Alexa Fluor® 488 & DyLight® 488
Molecular Weight 1260

The post AQuora® 488-Maleimide appeared first on VectorLabs.

]]>

Description

AQuora® 488-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® 488-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 488-Maleimide is a xanthene-based dye with photophysical properties comparable to Alexa Fluor 488® and DyLight® 488. It is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond. (Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.)

Specifications

Unit Size1mg
FormatLyophilized
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® 488
Excitation / Emission Maximum493 nm / 522 nm
A280 Correction Factor0.110
Reactive GroupMaleimide
Extinction Coefficient73,000
Spectrally Similar DyesAlexa Fluor® 488 & DyLight® 488
Molecular Weight1260

Documents

The post AQuora® 488-Maleimide appeared first on VectorLabs.

]]> DBCO-dPEG®₁₂-carboxyfluorescein https://staging.vectorlabs.com/products/dbco-dpeg12-carboxyfluorescein/ Sat, 24 Feb 2024 02:18:07 +0000 https://staging.vectorlabs.com/?post_type=product&p=39845 DBCO-dPEG®12-carboxyfluorescein, product number QBD-11812, permits fluorescein labeling of peptides, proteins, and other molecules using strain-promoted azide-alkyne cycloaddition (SPAAC). This compound combines the xanthene dye, 5(6)-carboxyfluorescein, with the popular click chemistry alkyne dibenzylcyclooctyne (DBCO) through a medium-length (40 atoms, 47.6 Å), single molecular weight, discrete PEG (dPEG®) spacer. The carboxyfluorescein label has an excitation wavelength (λex) of 498 nanometers (nm), and an emission wavelength (λem) of 528 nm. The molar absorptivity (ε) is 7.96×104 M-1 cm-1 at pH 8.0 and 498 nm.

Fluorescein and its derivatives are among the most popular dyes in use today for labeling proteins and cells. Unfortunately, these dyes are hydrophobic, and care must be taken to avoid over-labeling because over-labeling can result in quenching and possibly aggregation and precipitation of the conjugates through non-specific hydrophobic interactions.

DBCO is an increasingly popular choice for SPAAC chemistry, also known as ""copper-free click chemistry"" because it avoids the use of cytotoxic copper(I) ions and allows reactions to occur in and with living cells. Although DBCO may react with free sulfhydryl groups under some conditions, it is highly selective for azides. Because azides must be introduced artificially into proteins, DBCO is considered a bioorthogonal click chemistry reagent.

The introduction of a flexible dPEG® spacer between carboxyfluorescein and DBCO increases the molecule's hydrodynamic volume and imparts water solubility to DBCO-dPEG®12-carboxyfluorescein. Thus, DBCO-dPEG®12-carboxyfluorescein provides a highly selective, flexible, water-soluble way to label proteins, peptides, and cells with carboxyfluorescein using a bio-orthogonal reagent designed to simplify analysis and characterization of the conjugate.

Specifications

Unit Size 1 mg, 5 mg
Molecular Weight 1234.34; single compound
Chemical formula C₆₆H₇₉N₃O₂₀
CAS N/A
Purity > 97%
Spacers dPEG® Spacer is 40 atoms and 47.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post DBCO-dPEG®₁₂-carboxyfluorescein appeared first on VectorLabs.

]]>

Description

DBCO-dPEG®12-carboxyfluorescein, product number QBD-11812, permits fluorescein labeling of peptides, proteins, and other molecules using strain-promoted azide-alkyne cycloaddition (SPAAC). This compound combines the xanthene dye, 5(6)-carboxyfluorescein, with the popular click chemistry alkyne dibenzylcyclooctyne (DBCO) through a medium-length (40 atoms, 47.6 Å), single molecular weight, discrete PEG (dPEG®) spacer. The carboxyfluorescein label has an excitation wavelength (λex) of 498 nanometers (nm), and an emission wavelength (λem) of 528 nm. The molar absorptivity (ε) is 7.96×104 M-1 cm-1 at pH 8.0 and 498 nm.

Fluorescein and its derivatives are among the most popular dyes in use today for labeling proteins and cells. Unfortunately, these dyes are hydrophobic, and care must be taken to avoid over-labeling because over-labeling can result in quenching and possibly aggregation and precipitation of the conjugates through non-specific hydrophobic interactions.

DBCO is an increasingly popular choice for SPAAC chemistry, also known as “”copper-free click chemistry”” because it avoids the use of cytotoxic copper(I) ions and allows reactions to occur in and with living cells. Although DBCO may react with free sulfhydryl groups under some conditions, it is highly selective for azides. Because azides must be introduced artificially into proteins, DBCO is considered a bioorthogonal click chemistry reagent.

The introduction of a flexible dPEG® spacer between carboxyfluorescein and DBCO increases the molecule’s hydrodynamic volume and imparts water solubility to DBCO-dPEG®12-carboxyfluorescein. Thus, DBCO-dPEG®12-carboxyfluorescein provides a highly selective, flexible, water-soluble way to label proteins, peptides, and cells with carboxyfluorescein using a bio-orthogonal reagent designed to simplify analysis and characterization of the conjugate.

Specifications

Unit Size1 mg, 5 mg
Molecular Weight1234.34; single compound
Chemical formulaC₆₆H₇₉N₃O₂₀
CASN/A
Purity> 97%
SpacersdPEG® Spacer is 40 atoms and 47.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

The post DBCO-dPEG®₁₂-carboxyfluorescein appeared first on VectorLabs.

]]> AQuora® Fluorescein-Maleimide https://staging.vectorlabs.com/products/aquora-fluorescein-maleimide/ Sat, 24 Feb 2024 02:17:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=39752 AQuora® Fluorescein-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein-Maleimide is a xanthene-based dye with photophysical properties comparable to FITC and Carboxyfluorescein.

This AQuora® Fluorescein-Maleimide dye is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond.

Specifications

Unit Size 1mg
Format Solid
Storage Store at -20°C. Protect from light. May ship at ambient temperatures.
Country of Origin United States of America
Label AQuora® Fluorescein
Excitation / Emission Maximum 495 nm / 524 nm
A280 Correction Factor 0.190
Reactive Group Maleimide
Extinction Coefficient 76,000
Spectrally Similar Dyes FITC and Carboxyfluorescein
Molecular Weight 1060

The post AQuora® Fluorescein-Maleimide appeared first on VectorLabs.

]]>

Description

AQuora® Fluorescein-Maleimide is a thiol-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein-Maleimide yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein-Maleimide is a xanthene-based dye with photophysical properties comparable to FITC and Carboxyfluorescein.

This AQuora® Fluorescein-Maleimide dye is activated with a maleimide (MAL) group, which reacts with a thiol to form a thioether bond.

Specifications

Unit Size1mg
FormatSolid
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® Fluorescein
Excitation / Emission Maximum495 nm / 524 nm
A280 Correction Factor0.190
Reactive GroupMaleimide
Extinction Coefficient76,000
Spectrally Similar DyesFITC and Carboxyfluorescein
Molecular Weight1060

Documents

The post AQuora® Fluorescein-Maleimide appeared first on VectorLabs.

]]> AQuora® 488-NHS Ester https://staging.vectorlabs.com/products/aquora-488-nhs-ester/ Sat, 24 Feb 2024 02:14:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=37390 AQuora® 488-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® 488-NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 488-NHS Ester is a xanthene-based dye with photophysical properties comparable to Alexa Fluor® 488 & DyLight® 488.

This AQuora® 488-NHS Ester dye is activated with a N-hydroxysuccinimide (NHS) ester group which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Specifications

Unit Size 1mg
Format Solid
Storage Store at -20°C. Protect from light. May ship at ambient temperatures.
Country of Origin United States of America
Label AQuora® 488
Excitation / Emission Maximum 493 nm / 522 nm
A280 Correction Factor 0.110
Reactive Group NHS Ester
Extinction Coefficient 73,000
Spectrally Similar Dyes Alexa Fluor® 488 & DyLight® 488
Molecular Weight 1250

The post AQuora® 488-NHS Ester appeared first on VectorLabs.

]]>

Description

AQuora® 488-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® 488-NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® 488-NHS Ester is a xanthene-based dye with photophysical properties comparable to Alexa Fluor® 488 & DyLight® 488.

This AQuora® 488-NHS Ester dye is activated with a N-hydroxysuccinimide (NHS) ester group which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Alexa Fluor® and DyLight® are registered trademarks of Thermo Fisher Scientific.

Specifications

Unit Size1mg
FormatSolid
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® 488
Excitation / Emission Maximum493 nm / 522 nm
A280 Correction Factor0.110
Reactive GroupNHS Ester
Extinction Coefficient73,000
Spectrally Similar DyesAlexa Fluor® 488 & DyLight® 488
Molecular Weight1250

Documents

The post AQuora® 488-NHS Ester appeared first on VectorLabs.

]]> AQuora® Fluorescein-NHS Ester https://staging.vectorlabs.com/products/aquora-fluorescein-nhs-ester/ Sat, 24 Feb 2024 02:13:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=37087 AQuora® Fluorescein-NHS Ester is a xanthene-based, amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. It is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Description

"AQuora® Fluorescein-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein NHS Ester is a xanthene-based dye with photophysical properties comparable to FITC, carboxyfluorescein, and Alexa Fluor® 488 (absorption maximum = 495 nm, emission maximum = 524 nm).
This AQuora® Fluorescein NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific."

Specifications

Unit Size 1mg
Format Solid
Storage Store at -20°C. Protect from light. May ship at ambient temperatures.
Country of Origin United States of America
Label AQuora® Fluorescein
Excitation / Emission Maximum 495 nm / 524 nm
A280 Correction Factor 0.190
Reactive Group NHS Ester
Extinction Coefficient 76,000
Spectrally Similar Dyes FITC and Carboxyfluorescein
Molecular Weight 1070

The post AQuora® Fluorescein-NHS Ester appeared first on VectorLabs.

]]>

AQuora® Fluorescein-NHS Ester is a xanthene-based, amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye-labeled conjugate. As a result, the dye-labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications, including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. It is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Description

“AQuora® Fluorescein-NHS Ester is an amine-reactive fluorophore engineered with SuperHydrophilic™ technology designed to improve solubility during labeling and of the dye labeled conjugate. As a result, the dye labeled conjugates made with AQuora® Fluorescein NHS Ester yield enhanced signal and signal-to-noise ratios in fluorescence-based applications including fluorescent western blotting, fluorescence-based microscopy, flow cytometry, and cell-based assays. AQuora® Fluorescein NHS Ester is a xanthene-based dye with photophysical properties comparable to FITC, carboxyfluorescein, and Alexa Fluor® 488 (absorption maximum = 495 nm, emission maximum = 524 nm).
This AQuora® Fluorescein NHS Ester dye is activated with an N-hydroxysuccinimide (NHS) ester group, which reacts with free primary amines, such as the amines on the side chain of lysines, forming a stable amide bond.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.”

Specifications

Unit Size1mg
FormatSolid
StorageStore at -20°C. Protect from light. May ship at ambient temperatures.
Country of OriginUnited States of America
LabelAQuora® Fluorescein
Excitation / Emission Maximum495 nm / 524 nm
A280 Correction Factor0.190
Reactive GroupNHS Ester
Extinction Coefficient76,000
Spectrally Similar DyesFITC and Carboxyfluorescein
Molecular Weight1070

Documents

The post AQuora® Fluorescein-NHS Ester appeared first on VectorLabs.

]]> Difluorocarboxyfluorescein Azide, 6-isomer https://staging.vectorlabs.com/products/difluorocarboxyfluorescein-azide-6-isomer/ Tue, 19 Sep 2023 23:13:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=23436 Description

2′, 7′-Difluorocarboxyfluorescein Azide, 6-isomer (also under Oregon Green 488 Azide (Oregon Green™ 6-Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), 6-isomer name) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

2′,7′-Difluorocarboxyfluorescein fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

[caption id="attachment_27819" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Alkynes, cyclooctynes
Abs/Em Maxima 496/524 nm
Extinction coefficient 78,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 637.68
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post Difluorocarboxyfluorescein Azide, 6-isomer appeared first on VectorLabs.

]]>

Description

488nm laser

2′, 7′-Difluorocarboxyfluorescein Azide, 6-isomer (also under Oregon Green 488 Azide (Oregon Green™ 6-Carboxamido-(6-Azidohexanyl), Triethylammonium Salt), 6-isomer name) is a bright, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

2′,7′-Difluorocarboxyfluorescein fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAlkynes, cyclooctynes
Abs/Em Maxima496/524 nm
Extinction coefficient78,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight637.68
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB488

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post Difluorocarboxyfluorescein Azide, 6-isomer appeared first on VectorLabs.

]]> AZDye 488 Hydroxylamine https://staging.vectorlabs.com/products/azdye-488-hydroxylamine/ Tue, 19 Sep 2023 22:56:24 +0000 https://staging.vectorlabs.com/?post_type=product&p=23405 Description

AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

AZDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

 

 

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Aldehyde, ketone
Abs/Em Maxima 494/517 nm
Extinction coefficient 73,000 cm-1M-1
Solubility Water, DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, CF™ 488
Molecular weight 606.58 (protonated)
Storage Conditions -20°C.

The post AZDye 488 Hydroxylamine appeared first on VectorLabs.

]]>

Description

488nm laser

AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

AZDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityAldehyde, ketone
Abs/Em Maxima494/517 nm
Extinction coefficient73,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, CF™ 488
Molecular weight606.58 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

The post AZDye 488 Hydroxylamine appeared first on VectorLabs.

]]> AZDye 488 Hydrazide https://staging.vectorlabs.com/products/azdye-488-hydrazide/ Tue, 19 Sep 2023 22:56:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=23401 Description

AZDye™ 488 Hydrazide is a bright, green-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AZDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.

AZDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

 

 

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Reactivity Aldehyde, ketone,
Abs/Em Maxima 494/517 nm
Extinction coefficient 73,000 cm-1M-1
Solubility Water, DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 548.50 (protonated)
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post AZDye 488 Hydrazide appeared first on VectorLabs.

]]>

Description

488nm laser

AZDye™ 488 Hydrazide is a bright, green-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AZDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.

AZDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size1 mg, 5 mg, 25 mg
ReactivityAldehyde, ketone,
Abs/Em Maxima494/517 nm
Extinction coefficient73,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight548.50 (protonated)
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

The post AZDye 488 Hydrazide appeared first on VectorLabs.

]]> Difluorocarboxyfluorescein Cadaverine, 5-isomer https://staging.vectorlabs.com/products/difluorocarboxyfluorescein-cadaverine-5-isomer/ Tue, 19 Sep 2023 22:56:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=23397 Description

Difluorocarboxyfluorescein Cadaverine, 5-isomer (sold under Oregon Green™ 488 Cadaverine, 5-isomer name) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

Difluorocarboxyfluorescein Cadaverineis a fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Difluorocarboxyfluorescein Cadaverine, 5-isomer structurally is identical to Invitrogen’s Oregon Green™ 488 Cadaverine, 5-isomer

Abs/Em Spectra

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity Aldehyde, carboxylic acid, ketone
Abs/Em Maxima 494/517 nm
Extinction coefficient 73,000 cm-1M-1
Solubility DMSO, DMF, MeOH
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 496.46
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post Difluorocarboxyfluorescein Cadaverine, 5-isomer appeared first on VectorLabs.

]]>

Description

488nm laser

Difluorocarboxyfluorescein Cadaverine, 5-isomer (sold under Oregon Green™ 488 Cadaverine, 5-isomer name) is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

Difluorocarboxyfluorescein Cadaverineis a fluorinated analog of fluorescein that overcomes some of the key limitations of fluorescein, including greater photostability and a lower pKa (pKa ~ 4.7 versus 6.4 for fluorescein), making its fluorescence essentially pH insensitive in the physiological pH range.

Difluorocarboxyfluorescein Cadaverine, 5-isomer structurally is identical to Invitrogen’s Oregon Green™ 488 Cadaverine, 5-isomer

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityAldehyde, carboxylic acid, ketone
Abs/Em Maxima494/517 nm
Extinction coefficient73,000 cm-1M-1
SolubilityDMSO, DMF, MeOH
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight496.46
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

MB488

Documents

The post Difluorocarboxyfluorescein Cadaverine, 5-isomer appeared first on VectorLabs.

]]> 5(6)-Carboxyrhodamine 110 NHS Ester https://staging.vectorlabs.com/products/56-carboxyrhodamine-110-nhs-ester/ Tue, 19 Sep 2023 22:49:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=23317 Description

5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.

The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

[caption id="attachment_27819" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity Primary amine
Abs/Em Maxima 502/527 nm
Extinction coefficient 76,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 471.43
Storage Conditions -50°C to -85°C
Shipping Conditions Dry ice

The post 5(6)-Carboxyrhodamine 110 NHS Ester appeared first on VectorLabs.

]]>

Description

488nm laser

5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.

The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima502/527 nm
Extinction coefficient76,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight471.43
Storage Conditions-50°C to -85°C
Shipping ConditionsDry ice

Abs/Em Spectra

MB488

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post 5(6)-Carboxyrhodamine 110 NHS Ester appeared first on VectorLabs.

]]>