PB Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

PB (structurally equivalent to Pacific Blue® Dye) is a moderately photostable, blue-fluorescent probe optimally excited by the 405 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

Pacific Blue® Dye is a registered trademark of Thermo Fisher Scientific.

[caption id="attachment_26250" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

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AZDye™ 430 Alkyne is a green-fluorescent alkyne-activated probe routinely used for imaging of moderate to high abundance azide-containing biomolecules. AZDye™ 430 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.

AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

For application where the presence of copper is not acceptable, please consider our AZDye™ 430 DBCO probe for copper-less detection of azide-modified molecules.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

[caption id="attachment_25397" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 430 Alkyne appeared first on VectorLabs.

AZDye™ 430 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

[caption id="attachment_25397" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 430 Picolyl Azide appeared first on VectorLabs.

AZDye™ 430 Azide is a water-soluble, green-fluorescent azide-activated probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free click chemistry reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

[caption id="attachment_25397" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 430 Azide appeared first on VectorLabs.

AZDye™ 430 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.

AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.

AZDye™ 430 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

[caption id="attachment_25397" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

The post AZDye 430 DBCO appeared first on VectorLabs.