1306 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:07 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 1306 – VectorLabs https://staging.vectorlabs.com 32 32 Cy3 Alkyne https://staging.vectorlabs.com/products/cy3-alkyne/ Tue, 19 Sep 2023 19:13:15 +0000 https://staging.vectorlabs.com/?post_type=product&p=21705 Description

Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

[caption id="attachment_24113" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima 553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar Dyes Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight 761.92 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Purity >95% (HPLC)
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

Cy3 Alkyne can be reacted with azides via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima553/569 nm
Extinction Coefficient
150,000
Flow Cytometry Laser Line
532, 555 or 568 nm
Microscopy Laser Line
532 or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549
Molecular weight761.92 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3

Documents

Selected References

  1. Banerjee, C., et al. (2022). Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET. J Vis Exp., 186. [PubMed]
  2. Schamber, M. R., et al. (2022). Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2. Elife, 11:e78982. [PubMed]
  3. Schamber, M. R., et al. (2022). Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nat Commun., 13 (1), 2194. [PubMed]

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