1305 – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:05:36 +0000 en-US hourly 1 https://wordpress.org/?v=6.9 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 1305 – VectorLabs https://staging.vectorlabs.com 32 32 Cy3 Picolyl Azide https://staging.vectorlabs.com/products/cy3-picolyl-azide/ Tue, 19 Sep 2023 17:57:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=21496 Description

Cy3 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

Cy3 Picolyl Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 dye is structurally similar, and spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

[caption id="attachment_24113" align="alignnone" width="661"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar Dyes Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight 927.08 (protonated)
CAS N/A
Solubility Water, DMSO, DMF
Purity >95% (HPLC)
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

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Description

Cy3 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

Cy3 Picolyl Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 dye is structurally similar, and spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima555/572 nm
Extinction Coefficient
155,000
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Spectrally Similar DyesAlexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight927.08 (protonated)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy3

Documents

Selected References

  1. Couradeau, E., et al. (2019). Probing the active fraction of soil microbiomes using BONCAT-FACS Nat Commun, 10, 2770-80. [PubMed]
  2. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  3. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  4. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
 

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