Mounting Media (IHC) – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:31 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Mounting Media (IHC) – VectorLabs https://staging.vectorlabs.com 32 32 VectaMount® Express Mounting Medium https://staging.vectorlabs.com/products/vectamount-express-mounting-medium/ Sun, 30 Apr 2023 02:54:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=13410 Description

VectaMount® Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and coverslip.

Features:

  • Non-aqueous clearing and mounting medium for IHC-stained slides
  • Eliminates the need for extensive ethanol washes and solvent-based clearing agents (e.g., xylene)
  • Rapid drying formula for fast visualization of stained samples
  • Preserves DAB staining for a minimum of 3 years and at least 18 months for other HRP and AP substrates at room temperature
  • Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as commonly used counterstains
  • Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging free of distortion or artifact

Specifications

Unit Size 60 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage VectaMount Express Mounting Medium is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following IHC staining. VectaMount Express is compatible with HRP and AP enzyme substrates, as well as commonly used counterstains.
Recommended Storage Room Temperature
Mounting Non-Aqueous
Antifade No
Safety Title WARNING: Reproductive Harm – www.P65Warnings.ca.gov

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Resources

Description

VectaMount® Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining. This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and coverslip. 

Features:

  • Non-aqueous clearing and mounting medium for IHC-stained slides
  • Eliminates the need for extensive ethanol washes and solvent-based clearing agents (e.g., xylene)
  • Rapid drying formula for fast visualization of stained samples
  • Preserves DAB staining for a minimum of 3 years and at least 18 months for other HRP and AP substrates at room temperature
  • Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as commonly used counterstains
  • Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging free of distortion or artifact

Specifications

Unit Size60 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageVectaMount Express Mounting Medium is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following IHC staining. VectaMount Express is compatible with HRP and AP enzyme substrates, as well as commonly used counterstains.
Recommended StorageRoom Temperature
MountingNon-Aqueous
AntifadeNo
Safety TitleWARNING: Reproductive Harm – www.P65Warnings.ca.gov

Documents

Product FAQs

It is important to use 99+% alcohol (isopropanol or ethanol) for the brief dehydration steps. Lower alcohol grades will not be viable substitutes. Slides should be rinsed in tap water (not buffer) prior to dehydration, as salts in buffers can precipitate if not removed. Depending on slide volume, alcohol baths should be changed out on a regular basis with fresh solution. It is suggested to drain excess water when transferring slides from tap water into the alcohol bath.

Usually the presence of air bubbles can be greatly reduced, and even eliminated, when using a manual approach, by carefully lowering the coverslip slowly over the specimen. Sometimes this may take a little practice to perfect.

No. It is recommended to dry slides at ambient room temperature.

There are no specific time limits for slides to be in the alcohol bath. Depending on the number of slides in a given assay, the last slide to be mounted will be in the alcohol bath longer than the first mounted slide. This may take several minutes or much longer. We have not seen any observable difference in specimen characteristics, staining or media performance with longer alcohol exposure times.

Place the slides in a glass staining rack or Coplin jar and completely submerge the slides in xylene overnight. After this time the coverslip should be easy to remove from the slide.

Yes. From our in-house testing, commonly used counterstains such as hematoxylin (e.g. Gill’s and Mayers formulations), nuclear fast red, methyl green and standard H&E (hematoxylin and eosin) stains are compatible with VectaMount Express Mounting Medium.

Technical Information

VectaMount Express Mounting Medium is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens, without the need for slide handling through an ethanol dehydration series and xylene clearing prior to coverslipping. VectaMount Express Mounting Medium preserves the color and intensity of preparations stained with enzyme substrates, such as DAB and BCIP/NBT, as well as our proprietary substrates Vector® NovaRED™ , Vector VIP, Vector SG, ImmPACT® DAB, ImmPACT DAB EqV, ImmPACT NovaRED, ImmPACT VIP, ImmPACT SG, Vector Red, ImmPACT Vector Red, and Vector Blue, for at least 18 months at room temperature. With a refractive index (1.49) closely matching that of the slide and tissue specimen, this mounting medium results in high-quality imaging free of distortion or artifact.

Preserves staining for at least 18 months

 ImmDAB VME Colon v2

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, ImmPACT DAB Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

ImmDAB VME Colon v1

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, ImmPACT DAB Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

DAB Ni VME Colon v1

Human Colon: Mouse Anti-Cytokeratin (AE1/AE3), ImmPRESS-HRP Anti-Mouse IgG, DAB-Ni Mounted in VectaMount Express Mounting Media and imaged after 18 months of storage.

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]]> VectaMount® AQ Aqueous Mounting Medium https://staging.vectorlabs.com/products/vectamount-aq-aqueous/ Thu, 27 Apr 2023 13:33:23 +0000 https://staging.vectorlabs.com/?post_type=product&p=12293 Description

VectaMount AQ Mounting Medium (H-5501) is a hardsetting mounting medium developed for use with enzymatic substrates, such as AEC, ImmPACT® AEC, and ImmPACT AMEC Red, whose reaction products are soluble in alcohol or other organic solvents. In applications where aqueous mounting is preferred, VectaMount AQ is suitable for use with other substrates such as DAB, Vector® SG, BCIP/NBT, Vector Red, ImmPACT Vector Red, Vector Blue, ImmPACT DAB, ImmPACT DAB EqV, and ImmPACT SG.

Features:

  • For aqueous mounting
  • Compatible with many horseradish peroxidase and alkaline phosphatase substrates

Specifications

Unit Size 60 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage VectaMount™ AQ Mounting Medium is a hard-setting aqueous-based mounting medium developed for use with enzymatic substrates such as AEC and ImmPACT™ AEC, whose reaction products are soluble in alcohol or other organic solvents. VectaMount AQ is also compatible with most peroxidase and alkaline phosphatase enzyme substrates, such as DAB, ImmPACT™ DAB, Vector® SG, ImmPACT™ SG, Vector® Red, Vector® Blue and BCIP/NBT.
Recommended Storage Room Temperature*
Mounting Aqueous (Hardening)
Antifade No

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Description

VectaMount AQ Mounting Medium (H-5501) is a hardsetting mounting medium developed for use with enzymatic substrates, such as AEC, ImmPACT® AEC, and ImmPACT AMEC Red, whose reaction products are soluble in alcohol or other organic solvents. In applications where aqueous mounting is preferred, VectaMount AQ is suitable for use with other substrates such as DAB, Vector® SG, BCIP/NBT, Vector Red, ImmPACT Vector Red, Vector Blue, ImmPACT DAB, ImmPACT DAB EqV, and ImmPACT SG.

Features:

  • For aqueous mounting
  • Compatible with many horseradish peroxidase and alkaline phosphatase substrates

Specifications

Unit Size60 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageVectaMount™ AQ Mounting Medium is a hard-setting aqueous-based mounting medium developed for use with enzymatic substrates such as AEC and ImmPACT™ AEC, whose reaction products are soluble in alcohol or other organic solvents. VectaMount AQ is also compatible with most peroxidase and alkaline phosphatase enzyme substrates, such as DAB, ImmPACT™ DAB, Vector® SG, ImmPACT™ SG, Vector® Red, Vector® Blue and BCIP/NBT.
Recommended StorageRoom Temperature*
MountingAqueous (Hardening)
AntifadeNo

Documents

Citations

Technical Information

VectaMount AQ is simple to use, requires no mixing, and preserves the color and clarity of the substrates. Stained sections mounted with VectaMount AQ can be stored in a slide box at room temperature for at least 2 years without fading.

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]]> VectaMount® PT Permanent Mounting Medium https://staging.vectorlabs.com/products/vectamount-pt-permanent/ Thu, 26 Jan 2023 15:19:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=12284 VectaMount PT Permanent Mounting Medium is a specially formulated, optically clear solution for permanent preservation of histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

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Description

VectaMount PT Permanent Mounting Medium is a specially formulated, optically clear solution for permanent preservation of histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.

Our mounting medium has undergone extensive testing and validation with all Vector Laboratories substrates and counterstains so you can confidently preserve your samples.

Features:

  • Permanent, Non-Aqueous Mounting: Provides stable, long-term preservation of specimens without the limitations associated with aqueous media.
  • Color Preservation: VectaMount PT has been validated with Vector Laboratories’ substrates and counterstains, preserving the vibrant colors over time without loss of intensity or color shifts.
  • Exceptional Optical Clarity: With an ideal refractive index (1.52), VectaMount PT ensures clear, artifact-free imaging.
  • Reliable Long-Term Stability: Retains consistent adhesive strength, cohesive integrity, and color stability even during extended storage periods.
  • Easy Application: Facilitates a uniform, thin mounting layer to enhance optical quality and simplify slide preparation.
  • Versatile Usage: Effectively secures coverslips to both thin and thick specimens.

Note: Highly flammable liquid and vapor. May be fatal if swallowed and enters airways. Causes skin irritation. Causes serious eye irritation.

VectaMount PT Permanent Mounting Medium (H-5600-60) is a different formulation than the discontinued VectaMount Permanent Mounting Medium (H-5000-60).

Specifications

Unit Size60 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage

VectaMount PT is for permanently preserving precipitable enzyme substrates in tissue sections or cell preparations.

Recommended StorageRoom Temperature
MountingNon-Aqueous (Permanent)
AntifadeNo

 

Documents

Technical Information

VectaMount PT Permanent Mounting Medium, specifically designed for effortless application and uniform distribution over tissue sections. Through extensive testing, we have confirmed that VectaMount PT reliably preserves the color and intensity of preparations stained with a variety of enzyme substrates, including DAB and BCIP/NBT, as well as our proprietary substrates such as Vector® NovaRED™, Vector VIP, Vector SG, ImmPACT® DAB, ImmPACT DAB EqV, ImmPACT NovaRED, ImmPACT VIP, ImmPACT SG, Vector Red, ImmPACT Vector Red, Vector Blue, and Vector Black. With VectaMount PT, you can confidently preserve the visual quality of your stained specimens.

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