Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA)  workflows.

Benefits include:

• Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections​
• Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows​
• Obtain more spatial biomarker information from each tissue section​
• Stain with multiple primaries from the same species and a single secondary without cross reactivity​
• Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
• Maintains tissue antigenicity through multiple cycles​
• Primary antibody concentrations do not need to be optimized ​based on the order of detection in multiplex workflows​

• Easy-to-use, room temperature application​

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG.  Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

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The Alcian Blue (pH 2.5) Stain Kit is intended for use in the histological visualization of sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins).

• Acidic Sulfated Mucosubstances: Blue
• Hyaluronic Acid: Blue
• Sialomucins: Blue
• Nuclei: Red

Control Tissue: Small Intestine, Appendix, Colon

Specifications

Components:

• Alcian Blue Solution (pH 2.5), 30 ml
• Nuclear Fast Red (Enhanced), 30 ml
• Acetic Acid Solution (3%), 60 ml (30 ml x 2)

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The Hematoxylin and Eosin Stain Kit is intended for use in histology and cytology applications. Included in this kit is a modified Eosin that provides the benefits of a traditional alcoholic formulation with significant improvements in usability. Advantages include lower evaporation rate, better color patterns, and improved surface tension to remain on tissue section. Our Hematoxylin produces crisp, intense blue nuclei providing optimal contrast to the Eosin stained cytoplasm.

Specifications

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The VECTABOND® Reagent for Tissue Section Adhesion is designed to significantly increase the adherence of both frozen and paraffin-embedded tissue sections and cell preparations to glass slides and coverslips. It chemically modifies the surface of the glass with positive charges to form a highly adherent surface.

Features:

• Tissues remain attached even under extreme conditions such as high temperature antigen unmasking techniques and in situ hybridization
• VECTABOND Reagent treated slides can be stored for long periods
• 7 ml concentrate dilutes to 350 ml
• Treats at least 500 standard slides

Specifications

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The ImmPrint Permanent Marking Pen is a solvent-resistant permanent marking pen that is designed for writing on frosted glass microscope slides, tissue cassettes, and most hard surfaces.

Features:

• High density, fast-drying, black ink is resistant to most organic solvents encountered in histological applications such as alcohols and clearing agents
• Has a smooth writing tip that resists drying out

Specifications

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Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.

Features:

• Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
• Suitable for use with nonaqueous and aqueous mountants
• Mercury-free

Specifications

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Nuclei stained with Vector^® Nuclear Fast Red (H-3403) have a pink appearance. Vector Nuclear Fast Red is an excellent choice in multiple labeling applications when hematoxylin obscures the substrate colors.

Features:

• Good contrast with a variety of substrates
• Rapid staining procedure requiring only one step
• Suitable for use with non-aqueous mountants

Specifications

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Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

• Simple, two-step procedure
• Suitable for use with a wide range of enzyme substrates
• Suitable for use with non-aqueous mountants

Specifications

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Vector Hematoxylin is based on Gill’s formulation, it produces blue stained nuclei with crisp detail.

Features:

• Alcohol and mercury free
• Progressive stain formula
• Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
• Suitable for use with non-aqueous and aqueous mountants

Specifications

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Vector Laboratories’ Antigen Unmasking Solutions are highly effective at revealing antigens in formalin-fixed, paraffin embedded tissue sections when used in combination with a high temperature treatment procedure. This Tris-based solution (H-3301) is pH 9.0 and is supplied as a convenient 100x concentrated stocks sufficient for preparation of 25 L of working solution.

Specifications

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