Counterstains – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:29 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Counterstains – VectorLabs https://staging.vectorlabs.com 32 32 Hematoxylin QS Counterstain https://staging.vectorlabs.com/products/vector-hematoxylin-qs-counterstain/ Wed, 26 Apr 2023 14:53:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=12257 Description

Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.

Features:

  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with nonaqueous and aqueous mountants
  • Mercury-free

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Hematoxylin QS Counterstain appeared first on VectorLabs.

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Description

Vector Hematoxylin QS (H-3404), a modification of Mayer’s hematoxylin developed especially for immunocytochemistry, provides crisp blue nuclear staining. This formula is ready-to-use without filtration, requires no separate bluing step, and stains in less than 45 seconds.

Features:

  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with nonaqueous and aqueous mountants
  • Mercury-free

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Hematoxylin QS Counterstain appeared first on VectorLabs.

]]> Nuclear Fast Red Counterstain https://staging.vectorlabs.com/products/vector-nuclear-fast-red-counterstain/ Wed, 26 Apr 2023 04:51:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=12244 Description

Nuclei stained with Vector® Nuclear Fast Red (H-3403) have a pink appearance. Vector Nuclear Fast Red is an excellent choice in multiple labeling applications when hematoxylin obscures the substrate colors.

Features:

  • Good contrast with a variety of substrates
  • Rapid staining procedure requiring only one step
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Nuclear Fast Red Counterstain appeared first on VectorLabs.

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Description

Nuclei stained with Vector® Nuclear Fast Red (H-3403) have a pink appearance. Vector Nuclear Fast Red is an excellent choice in multiple labeling applications when hematoxylin obscures the substrate colors.

Features:

  • Good contrast with a variety of substrates
  • Rapid staining procedure requiring only one step
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Optimize Counterstain Intensity

The post Nuclear Fast Red Counterstain appeared first on VectorLabs.

]]> Methyl Green Counterstain https://staging.vectorlabs.com/products/vector-methyl-green-counterstain/ Wed, 26 Apr 2023 04:45:58 +0000 https://staging.vectorlabs.com/?post_type=product&p=12234 Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Methyl Green Counterstain appeared first on VectorLabs.

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Description

Vector Methyl Green stains cell nuclei light green. It is an excellent alternative in multiple labeling when hematoxylin obscures the substrate colors.

Features:

  • Simple, two-step procedure
  • Suitable for use with a wide range of enzyme substrates
  • Suitable for use with non-aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Product FAQs

Methyl Green counterstain does take a little bit of optimization in some applications. We would suggest heating a volume of the counterstain (~300 ml) to 60 °C and add to a Coplin jar or similar glass staining rack, and submerse the slides. This approach facilitates a better uptake of stain into the section compared with placing the slide on a heated surface and placing drops of stain onto the section. Following the incubation time (~3-5 min), remove slides and wash in tap water. Omit the acetone, acetic acid rinse step described in the instructions and move the slides directly into 95% ethanol for the dehydration, clearing and mounting process. This methodology will retain more stain in the nuclei and hence produce greater intensity.

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Methyl Green Counterstain appeared first on VectorLabs.

]]> Hematoxylin Counterstain https://staging.vectorlabs.com/products/vector-hematoxylin-counterstain/ Wed, 26 Apr 2023 04:39:20 +0000 https://staging.vectorlabs.com/?post_type=product&p=12220 Description

Vector Hematoxylin is based on Gill’s formulation, it produces blue stained nuclei with crisp detail.

Features:

  • Alcohol and mercury free
  • Progressive stain formula
  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mountants

Specifications

Unit Size 500 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Format Ready-to-Use

The post Hematoxylin Counterstain appeared first on VectorLabs.

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Description

Vector Hematoxylin is based on Gill’s formulation, it produces blue stained nuclei with crisp detail.

Features:

  • Alcohol and mercury free
  • Progressive stain formula
  • Provides excellent color contrast with most commonly used peroxidase and alkaline phosphatase substrates
  • Suitable for use with non-aqueous and aqueous mountants

Specifications

Unit Size500 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
FormatReady-to-Use

Documents

Citations

Technical Information

Counterstain/Substrate Compatibility Table

This table is designed as a reference to determine the optimal counterstain/substrate combination for your application.

cstaincomp jpg

Optimize Counterstain Intensity

The post Hematoxylin Counterstain appeared first on VectorLabs.

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