Protein Detection and Visualization – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 16:26:57 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Protein Detection and Visualization – VectorLabs https://staging.vectorlabs.com 32 32 Q-Bright® Extreme Chemiluminescent Western Blot Kit https://staging.vectorlabs.com/products/q-bright-extreme-chemiluminescent-western-blot-kit/ Sat, 24 Feb 2024 02:18:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=40045 Q-Bright® Extreme chemiluminescent western blot kit is our most sensitive luminol-based substrate for horseradish peroxidase (HRP) . Q-Bright® Extreme provides much greater sensitivity compared to Q-Bright® Endure and is comparable to many of the available commercial reagents providing mid- to low-femtogram levels of detection in western blot. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Extreme Substrate and Stable Peroxide.

Specifications

Unit Size 1 Kit
Format Solution
Storage Store at 4°C. Product may ship at ambient temperature.
Country of Origin United States of America
Sensitivity

The post Q-Bright® Extreme Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Extreme chemiluminescent western blot kit is our most sensitive luminol-based substrate for horseradish peroxidase (HRP) . Q-Bright® Extreme provides much greater sensitivity compared to Q-Bright® Endure and is comparable to many of the available commercial reagents providing mid- to low-femtogram levels of detection in western blot. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Extreme Substrate and Stable Peroxide.

Specifications

Unit Size1 Kit
FormatSolution
StorageStore at 4°C. Product may ship at ambient temperature.
Country of OriginUnited States of America
Sensitivity 

Documents

The post Q-Bright® Extreme Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]> Q-Bright® Endure Plus Chemiluminescent Western Blot Kit https://staging.vectorlabs.com/products/q-bright-endure-plus-chemiluminescent-western-blot-kit/ Sat, 24 Feb 2024 02:18:34 +0000 https://staging.vectorlabs.com/?post_type=product&p=40039 Q-Bright® Endure Plus chemiluminescent western blot kit is a luminol-based substrate for horseradish peroxidase (HRP), providing 2X the signal of Q-Bright® Endure without compromising on signal duration, providing long-lasting, high sensitivity detection in chemiluminescent western blots. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Endure Plus Substrate and Stable Peroxide.

Specifications

Unit Size 1 Kit
Format Solution
Storage Store at 4°C. Product may ship at ambient temperature.
Country of Origin United States of America
Sensitivity

The post Q-Bright® Endure Plus Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Endure Plus chemiluminescent western blot kit is a luminol-based substrate for horseradish peroxidase (HRP), providing 2X the signal of Q-Bright® Endure without compromising on signal duration, providing long-lasting, high sensitivity detection in chemiluminescent western blots. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Endure Plus Substrate and Stable Peroxide.

Specifications

Unit Size1 Kit
FormatSolution
StorageStore at 4°C. Product may ship at ambient temperature.
Country of OriginUnited States of America
Sensitivity 

Documents

The post Q-Bright® Endure Plus Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]> Q-Bright® Endure Chemiluminescent Western Blot Kit https://staging.vectorlabs.com/products/q-bright-endure-chemiluminescent-western-blot-kit/ Sat, 24 Feb 2024 02:18:33 +0000 https://staging.vectorlabs.com/?post_type=product&p=40033 Q-Bright® Endure chemiluminescent western blot kit is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure provides much greater sensitivity compared to the classical ECL reagents and is superior to many of the available commercial reagents providing long-lasting, high to mid-femtogram levels of detection in chemiluminescent-based western blots. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Endure Substrate and Stable Peroxide.

Specifications

Unit Size 1 Kit
Format Solution
Storage Store at 4°C. Product may ship at ambient temperature.
Country of Origin United States of America
Sensitivity

The post Q-Bright® Endure Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Endure chemiluminescent western blot kit is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure provides much greater sensitivity compared to the classical ECL reagents and is superior to many of the available commercial reagents providing long-lasting, high to mid-femtogram levels of detection in chemiluminescent-based western blots. The Western Blot Kit includes western blot wash buffer, Q-Bright® Western Blot Blocking Buffer, Anti-Mouse and Anti-Rabbit HRP conjugated secondary antibodies, and Q-Bright® Endure Substrate and Stable Peroxide.

Specifications

Unit Size1 Kit
FormatSolution
StorageStore at 4°C. Product may ship at ambient temperature.
Country of OriginUnited States of America
Sensitivity 

Documents

The post Q-Bright® Endure Chemiluminescent Western Blot Kit appeared first on VectorLabs.

]]> Q-Bright® Extreme Chemiluminescent Detection Kit https://staging.vectorlabs.com/products/q-bright-extreme-chemiluminescent-detection-kit/ Sat, 24 Feb 2024 02:18:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=40024 Q-Bright® Extreme chemiluminescent detection reagent is a high-sensitivity, luminol-based substrate for horseradish peroxidase (HRP). Q-Bright® Extreme provides much greater sensitivity compared to Q-Bright® Endure and is comparable to many of the available commercial reagents providing mid- to low-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size 20 ml, 100 ml, 200 ml
Format Solution
Storage Store at 4°C.
Country of Origin United States of America
Sensitivity low to mid-femtogram

The post Q-Bright® Extreme Chemiluminescent Detection Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Extreme chemiluminescent detection reagent is a high-sensitivity, luminol-based substrate for horseradish peroxidase (HRP). Q-Bright® Extreme provides much greater sensitivity compared to Q-Bright® Endure and is comparable to many of the available commercial reagents providing mid- to low-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size20 ml, 100 ml, 200 ml
FormatSolution
StorageStore at 4°C.
Country of OriginUnited States of America
Sensitivitylow to mid-femtogram

Documents

The post Q-Bright® Extreme Chemiluminescent Detection Kit appeared first on VectorLabs.

]]> Q-Bright® Endure Plus Chemiluminescent Detection Kit https://staging.vectorlabs.com/products/q-bright-endure-plus-chemiluminescent-detection-kit/ Sat, 24 Feb 2024 02:18:31 +0000 https://staging.vectorlabs.com/?post_type=product&p=40015 Q-Bright® Endure Plus chemiluminescent detection reagent is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure Plus provides 2X the signal of Q-Bright® Endure without compromising on signal duration, providing long-lasting, mid-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size 20 ml, 100 ml, 200 ml
Format Solution
Storage Store at 4°C.
Country of Origin United States of America
Sensitivity mid-femtogram

The post Q-Bright® Endure Plus Chemiluminescent Detection Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Endure Plus chemiluminescent detection reagent is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure Plus provides 2X the signal of Q-Bright® Endure without compromising on signal duration, providing long-lasting, mid-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size20 ml, 100 ml, 200 ml
FormatSolution
StorageStore at 4°C.
Country of OriginUnited States of America
Sensitivitymid-femtogram

Documents

The post Q-Bright® Endure Plus Chemiluminescent Detection Kit appeared first on VectorLabs.

]]> Q-Bright® Endure Chemiluminescent Detection Kit https://staging.vectorlabs.com/products/q-bright-endure-chemiluminescent-detection-kit/ Sat, 24 Feb 2024 02:18:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=40006 Q-Bright® Endure chemiluminescent detection reagent is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure provides much greater sensitivity compared to the classical ECL reagents and is superior to many of the available commercial reagents providing long-lasting, high to mid-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size 20 ml, 100 ml, 200 ml
Format Solution
Storage Store at 4°C.
Country of Origin United States of America
Sensitivity high to mid-femtogram

The post Q-Bright® Endure Chemiluminescent Detection Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® Endure chemiluminescent detection reagent is a luminol-based substrate for horseradish peroxidase (HRP), providing a light output for up to 24h. Q-Bright® Endure provides much greater sensitivity compared to the classical ECL reagents and is superior to many of the available commercial reagents providing long-lasting, high to mid-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size20 ml, 100 ml, 200 ml
FormatSolution
StorageStore at 4°C.
Country of OriginUnited States of America
Sensitivityhigh to mid-femtogram

Documents

The post Q-Bright® Endure Chemiluminescent Detection Kit appeared first on VectorLabs.

]]> Q-Bright® ECL Plus Chemiluminescent Detection Kit https://staging.vectorlabs.com/products/q-bright-ecl-plus-chemiluminescent-detection-kit/ Sat, 24 Feb 2024 02:18:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=39997 Q-Bright® ECL Plus chemiluminescent detection kit is a luminol-based substrate for horseradish peroxidase (HRP) providing a more robust signal over the original ECL reagents. Q-Bright® ECL Plus provides picogram to high-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size 20 ml, 100 ml, 200 ml
Format Solution
Storage Store at 4°C. Product may ship at ambient temperature.
Country of Origin United States of America
Sensitivity Picogram to high-femtogram

The post Q-Bright® ECL Plus Chemiluminescent Detection Kit appeared first on VectorLabs.

]]>

Description

Q-Bright® ECL Plus chemiluminescent detection kit is a luminol-based substrate for horseradish peroxidase (HRP) providing a more robust signal over the original ECL reagents. Q-Bright® ECL Plus provides picogram to high-femtogram levels of detection in western blot, ELISA and other chemiluminescent-based applications.

Specifications

Unit Size20 ml, 100 ml, 200 ml
FormatSolution
StorageStore at 4°C. Product may ship at ambient temperature.
Country of OriginUnited States of America
SensitivityPicogram to high-femtogram

Documents

The post Q-Bright® ECL Plus Chemiluminescent Detection Kit appeared first on VectorLabs.

]]> VectaPlex™ Antibody Removal Kit https://staging.vectorlabs.com/products/vectaplex/ Wed, 28 Jun 2023 19:40:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=16641 Description

Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA)  workflows.

Benefits include:

  • Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections​
  • Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows​
  • Obtain more spatial biomarker information from each tissue section​
  • Stain with multiple primaries from the same species and a single secondary without cross reactivity​
  • Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
  • Maintains tissue antigenicity through multiple cycles​
  • Primary antibody concentrations do not need to be optimized ​based on the order of detection in multiplex workflows​
  • Easy-to-use, room temperature application​

 

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG.  Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

Unit Size 125 reactions /  kit
Applications Immunohistochemistry, Immunofluorescence, Tyramide Signal Amplification
Target Species Universal
Recommended Storage (15–25° C)
Format Ready-to-Use

The post VectaPlex™ Antibody Removal Kit appeared first on VectorLabs.

]]>

Description

Your path to simplified multiplexing starts with VectaPlex™.  This kit is designed to remove antibodies and other non-covalently bound detection reagents from FFPE tissue sections in immunohistochemistry (IHC), immunofluorescence (IF) and tyramide signal amplification (TSA)  workflows.

Benefits include:

  • Removes antibodies and other non-covalently bound detection reagents from FFPE tissue sections​
  • Compatible with immunofluorescence-, fluorescent tyramide- and immunohistochemistry-based workflows​
  • Obtain more spatial biomarker information from each tissue section​
  • Stain with multiple primaries from the same species and a single secondary without cross reactivity​
  • Maintains tissue morphology through multiple cycles – even on delicate specimens like breast & skin
  • Maintains tissue antigenicity through multiple cycles​
  • Primary antibody concentrations do not need to be optimized ​based on the order of detection in multiplex workflows​
  • Easy-to-use, room temperature application​

The first image above is a composite image generated from individual images taken during six rounds of immunofluorescence staining using VectaPlex.  FFPE stomach; six mouse primary antibodies – CD20 (red), CD34 (yellow), Desmin (cyan), AE1/AE3 (magenta), CD3 (gray), Vimentin (green) – detected with DyLight™ 488 horse anti-mouse IgG.  Blue indicates DAPI stain.  Vector® TrueView® Autofluorescence Quencher Kit applied to suppress background fluorescence.

The second image above is a 4-plex IHC image taken of esophagus tissue stained for CD68 (brown) using ImmPACT DAB, CD20 (red) using ImmPACT Vector Red, CD34 (blue) using Vector Blue, and Desmin (grey) using ImmPACT SG.  Each round of staining was performed using normal IHC protocols with VectaPlex treatment between each round of staining.

The third image above was captured after three rounds of tyramide staining. VectaPlex was used after each round. FFPE tonsil is shown, following application of 3 mouse primary antibodies – CD3 (red), CD68 (green) and AE1/AE3 (purple) – detected using Opal 3-plex TSA Manual Detection Kit. Blue indicates DAPI stain.

Specifications

Unit Size125 reactions /  kit
ApplicationsImmunohistochemistry, Immunofluorescence, Tyramide Signal Amplification
Target SpeciesUniversal
Recommended Storage(15–25° C)
FormatReady-to-Use

Documents

Technical Information

This ready-to-use kit is designed to remove antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunofluorescence or tyramide signal amplification staining workflows.

VectaPlex simplifies sample processing for manual workflows, accelerates multiplex assay optimization, preserves precious sample, and maintains tissue integrity and morphology through multiple cycles of antibody binding and removal.

 

 

VectaPlex IHC Workflow: After staining with one of the approved substrates, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. The substrate is not removed or impacted by VectaPlex. A single image is collected after all cycles are complete.

IHC Workflow Web

VectaPlex IF Workflow: After IF staining and imaging the sample, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. After all images have been collected, a composite image is formed from individual images.

IF Workflow web

VectaPlex TSA Workflow: After TSA staining and imaging the sample, VectaPlex is used to remove detection reagents in preparation for another cycle of staining. The tyramide sigal is not removed by VectaPlex. A single image is collected after all cycles are complete.

TSA workflow Web

The post VectaPlex™ Antibody Removal Kit appeared first on VectorLabs.

]]> TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) https://staging.vectorlabs.com/products/tmb-hrp-3-3-5-5-tetramethylbenzidine/ Sun, 21 May 2023 01:21:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=17687 Description

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.

Features:

  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s) Peroxidase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Microscopy Brightfield, Spectral Imaging
Mounting Non-Aqueous (Permanent)
Safety Title WARNING: Cancer – www.P65Warnings.ca.gov

What’s in the box:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4

The post TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) appeared first on VectorLabs.

]]>

Description

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.

Features:

  • Greater sensitivity than conventional substrates
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 300 ml of working solution

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s)Peroxidase
Substrate Reaction ProductPrecipitating Reaction Product
Chromogen ColorBlue
MicroscopyBrightfield, Spectral Imaging
MountingNon-Aqueous (Permanent)
Safety TitleWARNING: Cancer – www.P65Warnings.ca.gov

What’s in the box:

  • 6 ml TMB Reagent 1
  • 6 ml TMB Reagent 2
  • 6 ml TMB Reagent 3
  • 6 ml TMB Reagent 4

Documents

Technical Information

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.

Counterstain / Substrate Compatibility

This table is designed as a reference to determine the optimal counterstain / substrate combination for your application. Consideration should be given to tissue type, antigen unmasking protocol, and other detection parameters to achieve the desired staining intensity.

SubstrateCatalog NumberHematoxylin (H-3404)
Hematoxylin QS (H-3401)		Methyl Green (H-3402)Nuclear Fast Red (H-3404)
ImmPACT DAB (brown)SK-4105Excellent ContrastExcellent ContrastFair Contrast
ImmPACT DAB EqV (brown)SK-4103Excellent ContrastExcellent ContrastFair Contrast
DAB (brown)SK-4100Excellent ContrastExcellent ContrastFair Contrast
DAB-Ni (gray-black)SK-4100Excellent ContrastFair Contrast*Good Contrast
ImmPACT AEC (red)SK-4205Excellent ContrastCounterstain Incompatibility**Color Incompatibility
ImmPACT AMEC Red (red)SK-4285Excellent ContrastCounterstain Incompatibility**Color Incompatibility
AEC (red)SK-4200Excellent ContrastCounterstain Incompatibility**Color Incompatibility
TMB (blue)SK-4400Color IncompatibilityCounterstain IncompatibilityExcellent Contrast
ImmPACT VIP (purple)SK-4605Fair ContrastExcellent ContrastPoor Contrast
Vector VIP (purple)SK-4600Fair ContrastExcellent ContrastPoor Contrast
ImmPACT SG (blue-gray)SK-4705Poor ContrastGood ContrastExcellent Contrast
SG (blue-gray)SK-4700Poor ContrastGood ContrastExcellent Contrast
ImmPACT NovaRED (red)SK-4805Excellent ContrastExcellent Contrast***Color Incompatibility
Vector NovaRED (red)SK-4800Excellent ContrastExcellent Contrast***Color Incompatibility
ImmPACT Vector Red (magenta)SK-5105Excellent ContrastExcellent ContrastColor Incompatibility
Vector Red (magenta)SK-5100Excellent ContrastExcellent ContrastColor Incompatibility
Vector Black (black)SK-5200Excellent ContrastExcellent Contrast*Excellent Contrast
Vector Blue (blue)SK-5300Color IncompatibilityGood ContrastExcellent Contrast
BCIP / NBT (indigo)SK-5400Color IncompatibilityExcellent Contrast*Excellent Contrast
*This substrate shows a slight decrease in sensitivity following the methyl green protocol. This decrease can be minimized by reducing the heat incubation and acetone rinse times.
**Substrate dissolves in acetone wash.
***A slight color change in ImmPACT NovaRED and Vector NovaRED reaction produt may be seen using methyl green.

SUBSTRATEsensitivity2 jpg

The post TMB Substrate Kit, Peroxidase (HRP), (3,3′, 5,5′-tetramethylbenzidine) appeared first on VectorLabs.

]]> Animal-Free Blocker® and Diluent, R.T.U. https://staging.vectorlabs.com/products/animal-free-block-and-diluent-afb/ Thu, 04 May 2023 04:53:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=15151 Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

]]>

Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking ActionNon-Specific Protein Blocking
FormatReady-to-Use

Documents

Product FAQs

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Technical Information

This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. 

Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 

The post Animal-Free Blocker® and Diluent, R.T.U. appeared first on VectorLabs.

]]>