ImmPRESS Horse Radish Peroxidase (HRP) Polymer Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:19 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png ImmPRESS Horse Radish Peroxidase (HRP) Polymer Reagents – VectorLabs https://staging.vectorlabs.com 32 32 ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-mouse-igg-plus-kit/ Tue, 02 May 2023 20:35:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=14729 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Mouse IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-rabbit-igg-plus-kit/ Tue, 02 May 2023 20:31:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=14720 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides a more complete detection system.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-plus-horse-anti-mouse-rabbit-igg/ Tue, 02 May 2023 20:25:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=14712 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product.

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size 1 Kit
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

 

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. The Inclusion of BLOXALL® endogenous enzyme quenching solution and ImmPACT® DAB EqV substrate in the PLUS kit provides added convenience with optimized, matched reagents in one product. 

Features:

  • High sensitivity and low background
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Note: This polymer detection kit should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Specifications

Unit Size1 Kit
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Kit Contents:

  • 15 ml ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 15 ml 2.5% Normal Horse Serum for blocking (ready-to-use)
  • 15 ml BLOXALL Endogenous Enzyme Blocking Solution
  • ImmPACT DAB EqV Substrate (7.5 ml Reagent 1 + 7.5 ml Reagent 2)

Documents

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 
 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Universal (Horse Anti-Mouse/Rabbit IgG) PLUS Polymer Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent https://staging.vectorlabs.com/products/immpress-hrp-vr-horse-anti-mouse-igg-kit/ Tue, 02 May 2023 16:20:15 +0000 https://staging.vectorlabs.com/?post_type=product&p=14355 Description

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

ImmPRESS VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Minimal cross-reactivity with bovine, goat, sheep, pig, horse, cat, dog, and rabbit (as well as human). For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Specifications

 
Unit Size 15 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Format Ready-to-Use

The post ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

]]>

Description

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

ImmPRESS VR Polymer Detection Reagents are additionally cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Minimal cross-reactivity with bovine, goat, sheep, pig, horse, cat, dog, and rabbit (as well as human). For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Specifications

 
Unit Size15 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesMouse
FormatReady-to-Use

Kit Contents:

  • ImmPRESS VR Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)
  • The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

For mouse and rat tissues, separate kits are recommended (for rat, MP-7422; for mouse, MP-2400).

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 
Product review on Biocompare.com.
 

The post ImmPRESS®-VR Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

]]> ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-goat-anti-rabbit-igg-kit/ Tue, 02 May 2023 16:15:06 +0000 https://staging.vectorlabs.com/?post_type=product&p=14336 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme  is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in goat, ready-to-use)
  • 2.5% Normal Goat Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

The post ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme  is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in goat, ready-to-use)
  • 2.5% Normal Goat Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

 

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

The post ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-goat-igg-kit/ Tue, 02 May 2023 16:09:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=14325 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced staining of nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Goat IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Goat
Format Ready-to-Use

The post ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced staining of nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Suitable for use with goat, sheep, and bovine IgG primary antibodies

 

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Goat IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesGoat
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

The post ImmPRESS® HRP Horse Anti-Goat IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Universal Antibody (Horse Anti-Mouse/Rabbit IgG) Polymer Detection Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-anti-mouse-igg-anti-rabbit-igg/ Tue, 02 May 2023 15:37:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14226 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies.

 

Note: This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Kit Contents:

  • ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Universal (Mouse/Rabbit)
Format Ready-to-Use

The post ImmPRESS® HRP Universal Antibody (Horse Anti-Mouse/Rabbit IgG) Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • Designed for use with both rabbit and mouse primary antibodies. 

Note: This antibody should not be used to stain tissues from rodents or rabbits due to its reactivity with IgG present in the tissues of these species.

Kit Contents:

  • ImmPRESS Universal Antibody (anti-mouse IgG/anti-rabbit IgG, Peroxidase) Polymer Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesUniversal (Mouse/Rabbit)
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

 

The post ImmPRESS® HRP Universal Antibody (Horse Anti-Mouse/Rabbit IgG) Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-mouse-igg-kit/ Tue, 02 May 2023 15:27:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=14187 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Format Ready-to-Use

The post ImmPRESS® HRP Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Mouse IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)
The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections and the ImmPRESS (50 ml) Kit will stain approximately 250-500 sections.

Specifications

Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Mouse
Format Ready-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, the same ImmPRESS peroxidase secondary detection reagent can be used for double staining. There are a few considerations. First, we would suggest optimizing each single stain first on different tissue sections. Once the conditions for each stain have been established, the single assays should be run sequentially, from primary antibody though substrate color development, to achieve double staining on the same section. Secondly, different peroxidase substrates will have to be used to provide adequate color contrast and reliable target antigen localization. This double staining approach is most reproducible when detecting antigens expressed in different cell types on the same section, or different cell compartments of the same cell type. Please see our multiple antigen labeling guide for additional considerations:

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform: ImmPRESS App Note

Citations

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

The post ImmPRESS® HRP Horse Anti-Mouse IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase https://staging.vectorlabs.com/products/immpress-hrp-horse-anti-rabbit-igg/ Tue, 02 May 2023 14:56:41 +0000 https://staging.vectorlabs.com/?post_type=product&p=14172 Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step, non-biotin detection system for immunohistochemistry and immunocytochemistry staining. This unique micropolymer of highly active horseradish peroxidase (HRP) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • 15 ml kit will stain approximately 75-150 sections and the 50 ml kit will stain approximately 250-500 sections

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

Specifications

 
Unit Size 15 ml, 50 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

The post ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]>

Description

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step, non-biotin detection system for immunohistochemistry and immunocytochemistry staining. This unique micropolymer of highly active horseradish peroxidase (HRP) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.

Features:

  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling
  • 15 ml kit will stain approximately 75-150 sections and the 50 ml kit will stain approximately 250-500 sections

 

Kit Contents:

  • ImmPRESS (Peroxidase) Polymer Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

Specifications

 
Unit Size15 ml, 50 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Citations

Technical Information

The peroxidase micropolymers of the ImmPRESS HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Staining Procedure

The IHC immunostaining protocol is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

 

Consider Species Cross-Reactivity

When choosing the optimal detection system for your application, it is important to consider not only the species of the primary antibody but also the species of the tissue. If the species of the primary antibody and the species of the tissue are closely related (e.g. rat and mouse), the secondary antibody may bind to endogenous IgG in the tissue section leading to background. The following options minimize background staining in these instances:

  • Use a secondary antibody specifically adsorbed to remove cross-reacting antibodies of closely-related species (e.g. ImmPRESS Anti-Mouse IgG, Rat Adsorbed).
  • Use the M.O.M.® ImmPRESS Kit (Cat. No. MP-2400) for applications of mouse primary antibodies on mouse tissue.

Product review on Biocompare.com.

The post ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase appeared first on VectorLabs.

]]> ImmPRESS®-VR Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent https://staging.vectorlabs.com/products/enzyme-polymer-immpress-vr-horse-anti-rabbit-igg-hrp-kit/ Sat, 29 Apr 2023 18:21:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=13362 Description

ImmPRESS VR Polymer Detection Reagents are cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems. ImmPRESS VR Polymer HRP Anti-Rabbit IgG is designed to be used on the following tissues: bovine, goat, sheep, swine, horse, cat, dog, rat and mouse (as well as human).

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies.

Features:

  • High sensitivity
  • Low background
  • One-step detection
  • Micropolymer technology
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Specifications

Unit Size 15 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
Technology Micropolymer Reagents
Detection Enzyme(s) Peroxidase
Conjugate Micropolymer HRP
Target Species Rabbit
Format Ready-to-Use

Kit Contents:

  • ImmPRESS VR Polymer HRP Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

 

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

The post ImmPRESS®-VR Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

]]>

Resources

Description

ImmPRESS VR Polymer Detection Reagents are cross-adsorbed to ensure minimal cross-reactivity against endogenous tissue elements in animal species commonly used for diagnostics and research-based animal model systems. ImmPRESS VR Polymer HRP Anti-Rabbit IgG is designed to be used on the following tissues: bovine, goat, sheep, swine, horse, cat, dog, rat and mouse (as well as human).

The ImmPRESS Polymerized Reporter Enzyme Staining System is an enzymatic, one-step detection kit. ImmPRESS Polymer Reagents consist of unique micropolymers of highly active peroxidase enzyme attached to highly cross-adsorbed, affinity purified secondary antibodies. 

Features:

  • High sensitivity
  • Low background
  • One-step detection
  • Micropolymer technology
  • Enhanced accessibility to nuclear and membrane antigens
  • Ready-to-use in a convenient dropper bottle
  • Shorter assay time
  • Simplified multiple labeling

Specifications

Unit Size15 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, ELISAs
TechnologyMicropolymer Reagents
Detection Enzyme(s)Peroxidase
ConjugateMicropolymer HRP
Target SpeciesRabbit
FormatReady-to-Use

Kit Contents:

  • ImmPRESS VR Polymer HRP Anti-Rabbit IgG Reagent (made in horse, ready-to-use)
  • 2.5% Normal Horse Serum for blocking (ready-to-use)

The ImmPRESS (15 ml) Kit will stain approximately 75-150 sections.

Documents

Product FAQs

The ImmPRESS Excel kits are a two-step peroxidase polymer based detection system that include an unconjugated amplifier antibody. This intermediate amplifier antibody increases sensitivity of the assay at least three- to four-fold over that of a one-step polymer based system, by facilitating the introduction of more peroxidase enzyme at the site of specific antigen localization. This increase in sensitivity would be advantageous in instances of weak antigen expression, and to further dilute out an expensive primary antibody. The ImmPRESS Excel Amplifier kits are presented as a complete kit format that include enzyme quench, blocking serum and ImmPACT® DAB EqV peroxidase substrate, in addition to the amplifier antibody and defined ImmPRESS polymer secondary antibody. However, as the two published references below indicate, this format is still modular and allows for the substitution of different detection reagents. These references describe using ImmPACT NovaRED™ peroxidase substrate in combination with the ImmPRESS Excel Amplifier kits. Oncogenesis (2017) 6, e293; doi:10.1038/oncsis.2016.82 Tan, E.M.S., et al (2017) Front. Med. 4:162 (doi: 10.3389/fmed.2017.00162)

Yes, we conducted studies on the compatibility of the ImmPRESS HRP polymer reagents with three commercially available autostainers (Agilent/Dako Autostainer Plus, Leica Bond Rx, and Ventana Discovery Ultra). We showed that our reagents are suitable for IHC detection on each of these platforms. The ImmPRESS polymer reagents and enzyme substrates generated equivalent IHC staining results compared to reagents from the instrument manufacturer. Some modifications in the protocol were performed to optimize the signal-to-noise ratio, such as a shorter incubation time for the ImmPRESS polymer and increase in the number of buffer washes following polymer incubation. Please see the following application note for more details about each automated platform:https://staging.vectorlabs.com/wp-content/uploads/2022/11/VL_LIT3023_ImmPRESS_AppNote_4.pdf

Technical Information

The ImmPRESS Polymer Reagents produce outstanding immunohistochemistry and immunocytochemistry results due to increased target accessibility, binding specificity, and signal intensity along with low background staining.

Staining Procedure

The staining procedure is simple as shown in the diagram below. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice.

ImmPRESS SL600 jpg

Product review on Biocompare.com.

The post ImmPRESS®-VR Horse Anti-Rabbit IgG Polymer Detection Kit, Peroxidase, Veterinary Reagent appeared first on VectorLabs.

]]>