ABC Kits for Western Blot Detection – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:05 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png ABC Kits for Western Blot Detection – VectorLabs https://staging.vectorlabs.com 32 32 VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection) https://staging.vectorlabs.com/products/vectastain-amp-ap-reagent-western-blot/ Tue, 18 Apr 2023 20:16:02 +0000 https://staging.vectorlabs.com/?post_type=product&p=10725 Description

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

Features:

  • Uses biotin/streptavidin technology
  • Can detect as little as 1 pg target protein when used with DuoLuX Substrate
  • Standard Kit contains Reagent A and Reagent B only
  • Sufficient reagents to develop twenty 100 cm2 blots

Description

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

Features:

  • Uses biotin/streptavidin technology
  • Can detect as little as 1 pg target protein when used with DuoLuX Substrate
  • Standard Kit contains Reagent A and Reagent B only
  • Sufficient reagents to develop twenty 100 cm2 blots

Specifications

Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM galactose, 0.08% sodium azide, 0.01 mM MnCl2
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M N-acetylgalactoseamine in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Galactose, N-Acetylgalactosamine
Conjugate Agarose

Kit Contents:

  • 0.5 ml Reagent A and 0.5 ml Reagent B

Technical Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The post VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection) appeared first on VectorLabs.

]]>

Description

The VECTASTAIN ABC-AmP is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent / chemifluorescent or a chromogenic substrate. Used with the DuoLuX Chemiluminescent / Chemifluorescent Substrate, the VECTASTAIN ABC- AmP system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.

Features:

  • Uses biotin/streptavidin technology
  • Can detect as little as 1 pg target protein when used with DuoLuX Substrate
  • Standard Kit contains Reagent A and Reagent B only
  • Sufficient reagents to develop twenty 100 cm2 blots

Specifications

Unit Size1 kit
ApplicationsBlotting Applications, Elispot
TechnologyABC (Streptavidin/Biotin) Systems
Detection Enzyme(s)Alkaline Phosphatase
Target SpeciesNo antibody included
FormatConcentrate

Kit Contents:

  • 0.5 ml Reagent A and 0.5 ml Reagent B

Documents

Citations

Technical Information

Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.

The post VECTASTAIN® ABC-AmP Reagent (Standard, Western Blot Detection) appeared first on VectorLabs.

]]>