Western Blot Blocking Solutions – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 16:26:55 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Western Blot Blocking Solutions – VectorLabs https://staging.vectorlabs.com 32 32 WestVision™ Block and Diluent for Western Blots https://staging.vectorlabs.com/products/westvision-block-and-diluent-for-western-blots/ Tue, 02 May 2023 18:40:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14472 Description

WestVision™ Block and Diluent is a ready-to-use Tris buffer-based blocking solution intended for Western or dot blot applications. This solution is a proprietary formulation containing Tween 20 that improves signal intensity and resolution without increasing background. To achieve the best results, use WestVision™ Block and Diluent for all blocking and diluting steps including primary antibody and secondary antibody detection reagents. WestVision™ Block and Diluent is compatible with both alkaline phosphatase- and peroxidase- based detections systems, as well as with both chemiluminescence and chromogenic development.

Specifications

Unit Size 500 ml
Applications Blotting Applications, Elispot
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

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Description

WestVision™ Block and Diluent is a ready-to-use Tris buffer-based blocking solution intended for Western or dot blot applications. This solution is a proprietary formulation containing Tween 20 that improves signal intensity and resolution without increasing background. To achieve the best results, use WestVision™ Block and Diluent for all blocking and diluting steps including primary antibody and secondary antibody detection reagents. WestVision™ Block and Diluent is compatible with both alkaline phosphatase- and peroxidase- based detections systems, as well as with both chemiluminescence and chromogenic development.

Specifications

Unit Size500 ml
ApplicationsBlotting Applications, Elispot
Blocking ActionNon-Specific Protein Blocking
FormatReady-to-Use

Documents

Technical Information

To maximize the signal to noise ratio, the correct dilutions of primary antibody and secondary antibody conjugate need to be empirically determined and depend on several factors including the membrane type, the substrate to be used, the blocking/diluent solution and the amount of target. WestVision™ Block and Diluent is intended to be used as supplied without further dilution.

Due to the high sensitivity provided by the WestVision™ Block and Diluent, lower concentration of primary antibody and secondary antibody conjugates may be required to achieve optimal results.

As a starting point for chemiluminescent detection, dilute the primary antibody to 0.1 to 1.0 ug/ml and dilute the secondary antibody conjugate to 0.02 – 0.2 ug/ml, in WestVision™ Block and Diluent. 

As a starting point for chromogenic detection, dilute the primary antibody to 0.25 to 1.0 ug/ml and dilute the secondary antibody conjugate to 0.2 – 1.0 ug/ml in WestVision™ Block and Diluent.

IMPORTANT: Not all blocking solutions are appropriate for all Western blot assays. Blocking solutions should be tested for compatibility in a given assay. 

PROCEDURE

  1. Remove the membrane from the transfer device.

  2. Rinse briefly with deionized (DI) H2O.

  3. Add enough WestVision™ Block and Diluent to completely cover the membrane and incubate with agitation for 30 – 60 minutes.

  4. Continue with your detection protocol using WestVision™ Block and Diluent to dilute primary antibody and detection reagents. 

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]]> Animal-Free Blocker®, 5X Concentrate https://staging.vectorlabs.com/products/animal-free-blocker-5x/ Tue, 02 May 2023 01:25:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=13847 Description

Animal-Free Blocker (5x) is a plant-derived agent and diluent for immunohistochemistry, nucleic acid and protein blotting, and other applications.  This reagent contains no animal-derived protein and can be used as an alternative to sera, BSA, casein, or non-fat dry milk.  It is supplied as 250 ml of a 5x concentrate.

Specifications

Unit Size 250 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as a general blocking agent/diluent for nucleic acid and protein blot applications.This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot developement. Prior to use, 5x Animal-Free Blocker™ should be diluted to 1x with distilled or deionized water.For prolonged storage of diluted Animal-Free Blocker™, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated.
Blocking Action Non-Specific Protein Blocking

The post Animal-Free Blocker®, 5X Concentrate appeared first on VectorLabs.

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Description

Animal-Free Blocker (5x) is a plant-derived agent and diluent for immunohistochemistry, nucleic acid and protein blotting, and other applications.  This reagent contains no animal-derived protein and can be used as an alternative to sera, BSA, casein, or non-fat dry milk.  It is supplied as 250 ml of a 5x concentrate.

Specifications

Unit Size250 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as a general blocking agent/diluent for nucleic acid and protein blot applications.This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot developement. Prior to use, 5x Animal-Free Blocker™ should be diluted to 1x with distilled or deionized water.For prolonged storage of diluted Animal-Free Blocker™, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated.
Blocking ActionNon-Specific Protein Blocking

Documents

Product FAQs

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Citations

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