Protein Blocking – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:42 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Protein Blocking – VectorLabs https://staging.vectorlabs.com 32 32 Animal-Free Blocker® and Diluent, R.T.U. https://staging.vectorlabs.com/products/animal-free-block-and-diluent-afb/ Thu, 04 May 2023 04:53:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=15151 Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking Action Non-Specific Protein Blocking
Format Ready-to-Use

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Description

R.T.U. Animal Free Blocker and Diluent is a ready-to-use solution designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This product contains no animal-derived proteins. The R.T.U. Animal Free Blocker and Diluent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used. 

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as an antibody diluent and a blocking reagent to stabilize diluted antibody solutions and diminish background due to non-specific binding in immunohistochemical applications. This reagent contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations when animal protein-based blocking solutions cause increased background or interfere with blot development. Primary and secondary antibodies can be diluted in this solution and stored at 4 ºC for up to a week.
Blocking ActionNon-Specific Protein Blocking
FormatReady-to-Use

Documents

Product FAQs

Both products are derived from the same plant protein and in some applications can be used interchangeably. At a high level, one is presented as a ready-to-use (RTU) format and the other a more economical concentrate. The key difference is that the RTU format has been specifically optimized for use in IHC and IF applications. The RTU format has a neutral pH suitable for tissue and cell-based assays and has been more highly refined to avoid the presence of flocculent material that may cause interference with microscopy work. The 5x concentrated format is best suited for membrane blotting applications.

Technical Information

This plant protein derived product is a universal antibody diluent and blocking reagent intended for cell and tissue based immunohistochemistry (IHC) and immunofluorescent (IF) applications. This ready-to-use solution can be used as an alternative to normal sera, BSA, casein and non-fat dry milk in methods where these or other blocking agents have been found to be ineffective.

It is supplied without any sodium azide, and as such, can be used with both peroxidase and alkaline phosphatase antibody conjugates and all secondary detection reagents including polymer systems and avidin/biotin reagents that incorporate these enzymes. 

Importantly, this is a different formulation than our concentrated (5x) animal free blocker, due to the optimized conditions and neutral pH of the solution for IHC and IF methods. 

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]]> Carbo-Free™ Blocking Solution (10x Concentrate) https://staging.vectorlabs.com/products/carbo-free-blocking-solution-10x-concentrate/ Tue, 02 May 2023 01:29:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=13855 Description

The Carbo-Free Blocking Solution is a protein-based agent used as a general blocking or diluent solution. It is ideal for applications using lectins in which glycoprotein contamination could generate background staining or false positive results.

Features:

  • Essentially free of glycoproteins, unlike other common protein-containing blocking agents like serum, nonfat dry milk, or casein
  • Supplied as 125 ml of 10x concentrate

Specifications

More Information
Unit Size 125 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Storage 2-8 °C
Recommended Usage Prior to use, 10x Carbo-Free Blocking Solution should be diluted to 1x with distilled or deionized water. For prolonged storage of the 1x solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated. Carbo-Free Blocking Solution does not contain detergent. In some applications, blocking may be enhanced by addition of Tween™ 20 to a final concentration of 0.05% to 0.1%, in the working solution.
Blocking Action Non-Specific Protein Blocking
Format Concentrate

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Description

The Carbo-Free Blocking Solution is a protein-based agent used as a general blocking or diluent solution. It is ideal for applications using lectins in which glycoprotein contamination could generate background staining or false positive results. 

Features:

  • Essentially free of glycoproteins, unlike other common protein-containing blocking agents like serum, nonfat dry milk, or casein
  • Supplied as 125 ml of 10x concentrate

Specifications

More Information
Unit Size125 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Storage2-8 °C
Recommended UsagePrior to use, 10x Carbo-Free Blocking Solution should be diluted to 1x with distilled or deionized water. For prolonged storage of the 1x solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated. Carbo-Free Blocking Solution does not contain detergent. In some applications, blocking may be enhanced by addition of Tween™ 20 to a final concentration of 0.05% to 0.1%, in the working solution.
Blocking ActionNon-Specific Protein Blocking
FormatConcentrate

Documents

Citations

Technical Information

A suggested protocol for detection of glycoconjugates using lectins/agglutinins is available under the “PROTOCOLS” tab.

The post Carbo-Free™ Blocking Solution (10x Concentrate) appeared first on VectorLabs.

]]> 10x Casein Solution https://staging.vectorlabs.com/products/10x-casein-solution/ Tue, 02 May 2023 01:22:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=13840 Description

10x Casein Solution is a general blocking agent for histochemical, nucleic acid and protein blotting, and other applications.  It is supplied as 250 ml of a 10x concentrate.

Specifications

Unit Size 250 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage 2-8 °C
Recommended Usage This product is designed to be used as a general blocking agent/diluent for nucleic acid and protein blotting applications.Prior to use, 10x Casein Solution should be diluted to 1x with distilled or deionized water.For prolonged storage of diluted Casein Solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated.
Blocking Action Non-Specific Protein Blocking
Format Concentrate

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Description

10x Casein Solution is a general blocking agent for histochemical, nucleic acid and protein blotting, and other applications.  It is supplied as 250 ml of a 10x concentrate.

Specifications

Unit Size250 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Storage2-8 °C
Recommended UsageThis product is designed to be used as a general blocking agent/diluent for nucleic acid and protein blotting applications.Prior to use, 10x Casein Solution should be diluted to 1x with distilled or deionized water.For prolonged storage of diluted Casein Solution, sodium azide should be added to give a final concentration of 0.08% (w/v) and the diluted solution refrigerated.
Blocking ActionNon-Specific Protein Blocking
FormatConcentrate

Documents

FAQs

Product SP-5020 is supplied as a stock concentrate (10x) of 5% casein (w/v). Once diluted as per the instructions the concentration at 1X is 0.5% casein.

Citations

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]]> Bovine Serum Albumin (BSA), Biotinylated https://staging.vectorlabs.com/products/biotinylated-bovine-serum-albumin-bsa/ Thu, 20 Apr 2023 19:32:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=11125 Description

This product is produced from our ultrapure immunohistochemical grade bovine serum albumin. This biotinylated protein can be used for measuring binding capacity of avidin or streptavidin matrices, as a reagent for amplifying biotin-avidin detection systems, or as a positive control in dot blot assays.

Specifications

Unit Size 10 mg
Applications Blotting Applications, Affinity Chromatography
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% azide.
Recommended Storage 2-8 °C
Concentration 5 mg/ml
Conjugate Biotinylated

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Description

This product is produced from our ultrapure immunohistochemical grade bovine serum albumin. This biotinylated protein can be used for measuring binding capacity of avidin or streptavidin matrices, as a reagent for amplifying biotin-avidin detection systems, or as a positive control in dot blot assays.

Specifications

Unit Size10 mg
ApplicationsBlotting Applications, Affinity Chromatography
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% azide.
Recommended Storage2-8 °C
Concentration5 mg/ml
ConjugateBiotinylated

Documents

Citations

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