Enzyme Blocking – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 16:26:54 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Enzyme Blocking – VectorLabs https://staging.vectorlabs.com 32 32 BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase https://staging.vectorlabs.com/products/bloxall-endogenous-peroxidase-and-ap/ Tue, 02 May 2023 01:36:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=13869 Description

BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations. This reagent is provided as a ready-to-use reagent in a convenient dropper bottle.

Features:

  • More effective than conventional blocking methods
  • Single 10 minute incubation step
  • 100 ml solution

Specifications

 
Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Neurobiology
Recommended Storage 2-8 °C
Recommended Usage BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations.
Blocking Action Endogenous Peroxidase, Endogenous Alkaline Phosphatase

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Description

BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations. This reagent is provided as a ready-to-use reagent in a convenient dropper bottle.

Features: 

  • More effective than conventional blocking methods
  • Single 10 minute incubation step
  • 100 ml solution

Specifications

 
Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Neurobiology
Recommended Storage2-8 °C
Recommended UsageBLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution is a dual enzyme blocking reagent that inactivates endogenous peroxidase, pseudoperoxidase, and alkaline phosphatase in formalin-fixed, paraffin-embedded tissue sections, frozen tissue sections, and cell preparations.
Blocking ActionEndogenous Peroxidase, Endogenous Alkaline Phosphatase

Documents

Citations

Technical Information

Tissues can contain endogenous peroxidase, pseudoperoxidase, and/or alkaline phosphatase activity that will produce background staining if an alkaline phosphatase and/or peroxidase detection system and corresponding substrates are used. 

The post BLOXALL® Endogenous Blocking Solution, Peroxidase and Alkaline Phosphatase appeared first on VectorLabs.

]]> Levamisole Solution https://staging.vectorlabs.com/products/levamisole-solution/ Tue, 02 May 2023 01:18:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=13833 Description

Levamisole Solution, a competitive inhibitor of alkaline phosphatase, is provided in a convenient dropper bottle and can be added to the alkaline phosphatase substrate solution. Tissues can contain endogenous alkaline phosphatase activity that will produce background staining if an alkaline phosphatase detection system and substrate are used.

Features:

  • Does not inhibit the isoenzyme used for alkaline phosphatase detection
  • 100x concentrate

Specifications

Unit Size 18 ml
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization
Recommended Storage 2-8 °C
Recommended Usage Levamisole is a reversible inhibitor of alkaline phosphatase, effective against most forms of alkaline phosphatase other than the intestinal isoenzyme. Since the intestinal form of the enzyme is used as the marker enzyme in the VECTASTAIN® ABC-AP Kit, levamisole can be used to inhibit endogenous alkaline phosphatase in most cases.
Concentration 125 mM
Blocking Action Endogenous Alkaline Phosphatase
Format Concentrate

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Description

Levamisole Solution, a competitive inhibitor of alkaline phosphatase, is provided in a convenient dropper bottle and can be added to the alkaline phosphatase substrate solution. Tissues can contain endogenous alkaline phosphatase activity that will produce background staining if an alkaline phosphatase detection system and substrate are used.

Features:

  • Does not inhibit the isoenzyme used for alkaline phosphatase detection
  • 100x concentrate

Specifications

Unit Size18 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization
Recommended Storage2-8 °C
Recommended UsageLevamisole is a reversible inhibitor of alkaline phosphatase, effective against most forms of alkaline phosphatase other than the intestinal isoenzyme. Since the intestinal form of the enzyme is used as the marker enzyme in the VECTASTAIN® ABC-AP Kit, levamisole can be used to inhibit endogenous alkaline phosphatase in most cases.
Concentration125 mM
Blocking ActionEndogenous Alkaline Phosphatase
FormatConcentrate

Documents

Citations

Technical Information

Levamisole does not inhibit the isoenzyme used for either the VECTASTAIN® ABC-AP reagents or other alkaline phosphatase conjugates. 

One drop of levamisole concentrate is added to 5 ml of substrate solution.

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