Fluorophore Conjugated – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:01:58 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Fluorophore Conjugated – VectorLabs https://staging.vectorlabs.com 32 32 Streptavidin, CY5 https://staging.vectorlabs.com/products/cy5-streptavidin/ Thu, 04 May 2023 04:12:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=15026 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 647 nm
Maximum Emission 663 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy5
Color of Fluorescence Far Red
Format Concentrate

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  650 nm
  • Emission:  670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation647 nm
Maximum Emission663 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy5
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

The post Streptavidin, CY5 appeared first on VectorLabs.

]]> Streptavidin, CY3 https://staging.vectorlabs.com/products/cy3-streptavidin/ Tue, 02 May 2023 19:24:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=14518 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 550 nm
Maximum Emission 570 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Cy3
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, CY3 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody. 

  • Excitation:  552 nm
  • Emission:  565 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use 5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation550 nm
Maximum Emission570 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateCy3
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

The post Streptavidin, CY3 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 594 https://staging.vectorlabs.com/products/dylight-594-streptavidin/ Mon, 01 May 2023 19:42:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=13589 Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 592 nm
Maximum Emission 617 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 594
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

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Description

Features:

  • Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
  • Highly purified and possess very low non-specific binding properties
  • Extremely high affinity for biotin
  • Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation at 592 nm
  • Emission at 617 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation592 nm
Maximum Emission617 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 594
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 594 appeared first on VectorLabs.

]]> Streptavidin, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-streptavidin/ Mon, 01 May 2023 19:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13562 Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 493 nm
Maximum Emission 518 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 488
Color of Fluorescence Green
Format Concentrate

The post Streptavidin, DyLight® 488 appeared first on VectorLabs.

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Description

DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. 

Features:

  • Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
  • Excitation maximum: 493 nm
  • Emission maximum: 518 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation493 nm
Maximum Emission518 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 488
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. 

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight® 488 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 549 https://staging.vectorlabs.com/products/dylight-549-streptavidin/ Sun, 30 Apr 2023 03:48:22 +0000 https://staging.vectorlabs.com/?post_type=product&p=13465 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 556 nm
Maximum Emission 571 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 549
Color of Fluorescence Orange
Format Concentrate

The post Streptavidin, DyLight™ 549 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possess very low non-specific binding properties
  • Excitation at 556 nm
  • Emission at 571 nm
  • Color:  Orange

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation556 nm
Maximum Emission571 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 549
Color of FluorescenceOrange
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

DyLightspectra jpg

Dylight Table1

The post Streptavidin, DyLight™ 549 appeared first on VectorLabs.

]]> Streptavidin, DyLight™ 649 https://staging.vectorlabs.com/products/dylight-649-streptavidin/ Fri, 28 Apr 2023 21:23:56 +0000 https://staging.vectorlabs.com/?post_type=product&p=13063 Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Excitation at 655 nm
  • Emission at 670 nm
  • Color:  Far Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage Recommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 655 nm
Maximum Emission 670 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate DyLight 649
Color of Fluorescence Far Red
Format Concentrate

The post Streptavidin, DyLight™ 649 appeared first on VectorLabs.

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Description

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Excitation at 655 nm
  • Emission at 670 nm
  • Color:  Far Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageRecommended concentration range for use5-30 µg/ml Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation655 nm
Maximum Emission670 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateDyLight 649
Color of FluorescenceFar Red
FormatConcentrate

Documents

Citations

Technical Information

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight™ dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight™ conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.

*Additional information about the images can be found here.

DyLightspectra jpg

Dylight Table1 jpg

The post Streptavidin, DyLight™ 649 appeared first on VectorLabs.

]]> Streptavidin, AMCA https://staging.vectorlabs.com/products/amca-streptavidin/ Fri, 28 Apr 2023 21:09:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=13032 Description

AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Features:

  • Recommended for routine immunofluorescence applications
  • Highly purified and possesses very low non-specific binding properties
  • Extremely high affinity for biotin
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage For diluting this product, we recommend a HEPES- or biocarbonate-buffered saline solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended concentration range for use is 10-30 µg/ml.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation 345-355 nm
Maximum Emission 448-454 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml
Conjugate AMCA
Color of Fluorescence Blue
Format Concentrate

The post Streptavidin, AMCA appeared first on VectorLabs.

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Description

AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Features:

  • Recommended for routine immunofluorescence applications
  • Highly purified and possesses very low non-specific binding properties
  • Extremely high affinity for biotin
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES- or biocarbonate-buffered saline solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended concentration range for use is 10-30 µg/ml.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation345-355 nm
Maximum Emission448-454 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml
ConjugateAMCA
Color of FluorescenceBlue
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Most paraffin-embedded tissues have little autofluorescence under those conditions. AMCA Streptavidin may provide an advantage in many labeling situations over conjugates of fluorescein and related dyes. AMCA Streptavidin can be used on frozen or paraffin-embedded sections as a single label or in combination with other fluorescent conjugates for multiple labeling studies.

The post Streptavidin, AMCA appeared first on VectorLabs.

]]> Streptavidin, Texas Red™ https://staging.vectorlabs.com/products/texas-red-streptavidin/ Fri, 28 Apr 2023 21:07:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=13025 Description

Texas Red Streptavidin is a highly fluorescent conjugate of streptavidin and sulforhodamine 101. It can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possesses very low non-specific binding properties
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum: 595 nm
  • Emission maximum: 615 nm
  • Color:  Red

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage The recommended concentration range for use is 5-30 µg/ml.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation 595-604 nm
Maximum Emission 606-615 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg/ml active conjugate
Conjugate Texas Red
Color of Fluorescence Red
Format Concentrate

The post Streptavidin, Texas Red™ appeared first on VectorLabs.

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Description

Texas Red Streptavidin is a highly fluorescent conjugate of streptavidin and sulforhodamine 101. It can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Features:

  • Highly purified and possesses very low non-specific binding properties
  • Has a high fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum: 595 nm
  • Emission maximum: 615 nm
  • Color:  Red

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageThe recommended concentration range for use is 5-30 µg/ml.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Recommended Storage2-8 °C
Concentration1.0 mg/ml active conjugate
ConjugateTexas Red
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Since the excitation and emission maxima are well separated from those of fluorescein, Texas Red Streptavidin can be employed with Fluorescein Streptavidin or other fluorescein conjugates to simultaneously localize two antigens in the same tissue section. Texas Red Streptavidin is also ideal for flow cytometry using instruments equipped with dye lasers.

The post Streptavidin, Texas Red™ appeared first on VectorLabs.

]]> Streptavidin, Fluorescein https://staging.vectorlabs.com/products/fluorescein-streptavidin/ Fri, 28 Apr 2023 20:59:29 +0000 https://staging.vectorlabs.com/?post_type=product&p=13008 Description

Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.

Features:

  • Recommended for routine immunofluorescence applications
  • Extremely high affinity for biotin
  • Has an optimal fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum at 495 nm
  • Emission maximum at about 515 nm
  • Color:  Green

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation 495-500 nm
Maximum Emission 514-521 nm
Recommended Storage 2-8 °C
Concentration 1.0 mg active conjugate/ml
Conjugate Fluorescein
Color of Fluorescence Green
Format Concentrate

The post Streptavidin, Fluorescein appeared first on VectorLabs.

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Description

Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.

Features:

  • Recommended for routine immunofluorescence applications
  • Extremely high affinity for biotin
  • Has an optimal fluorochrome to protein ratio
  • Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
  • Excitation maximum at 495 nm
  • Emission maximum at about 515 nm
  • Color:  Green

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation495-500 nm
Maximum Emission514-521 nm
Recommended Storage2-8 °C
Concentration1.0 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
FormatConcentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

The post Streptavidin, Fluorescein appeared first on VectorLabs.

]]> Texas Red™ Avidin DCS, Cell Sorting Grade https://staging.vectorlabs.com/products/texas-red-avidin-dcs-cell-sorting-grade/ Tue, 18 Apr 2023 16:59:04 +0000 https://staging.vectorlabs.com/?post_type=product&p=10504 Description

Avidin DCS, Texas Red® labeled, is a cell sorter grade of Texas Red® Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Texas Red® Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.

Specifications

Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentration for staining of cells or tissues is 5-20 µg/ml.
Solution 10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin
Maximum Excitation 595-604 nm
Maximum Emission 606-615 nm
Recommended Storage 2-8 °C
Concentration 2.0 mg active conjugate/ml
Conjugate Texas Red
Color of Fluorescence Red
Format Concentrate

Technical Information

Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D.
  • Excitation maximum:  595 nm
  • Emission maximum:  615 nm
  • Color:  Red

The post Texas Red™ Avidin DCS, Cell Sorting Grade appeared first on VectorLabs.

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Description

Avidin DCS, Texas Red® labeled, is a cell sorter grade of Texas Red® Avidin D. Through the development of a new method of labeling we are able to incorporate more fluorochrome into a protein without altering the properties of the protein. Not only does the Texas Red® Avidin DCS have a high F/P ratio, the efficiency of fluorescence is also increased. In addition to the improved conjugation technique, we employ a purification step to reduce nonspecific binding which can hamper resolution in cell sorting and other applications.

Specifications

Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended UsageFor diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentration for staining of cells or tissues is 5-20 µg/ml.
Solution10 mM HEPES, 0.5 M NaCl, pH 7.5, 0.08% sodium azide, 10 mg/ml beta cyclodextrin
Maximum Excitation595-604 nm
Maximum Emission606-615 nm
Recommended Storage2-8 °C
Concentration2.0 mg active conjugate/ml
ConjugateTexas Red
Color of FluorescenceRed
FormatConcentrate

Documents

Citations

Technical Information

Although this product has been designed for cell sorting, it can also be used in fluorescent microscopy when higher sensitivity and lower background staining are necessary. This derivative is preferred when using the amplification procedure with Biotinylated Anti-Avidin D.
 
  • Excitation maximum:  595 nm
  • Emission maximum:  615 nm
  • Color:  Red

The post Texas Red™ Avidin DCS, Cell Sorting Grade appeared first on VectorLabs.

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