Enzyme Conjugated – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:01:58 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Enzyme Conjugated – VectorLabs https://staging.vectorlabs.com 32 32 Streptavidin, Peroxidase, R.T.U. https://staging.vectorlabs.com/products/streptavidin-horseradish-peroxidase-ready-to-use/ Fri, 28 Apr 2023 21:28:38 +0000 https://staging.vectorlabs.com/?post_type=product&p=13073 Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size 100 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage For most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 µg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Ready-to-Use

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

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Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size100 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageFor most applications the recommended incubation time is 30 minutes. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. FOR LABORATORY USE.
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 µg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatReady-to-Use

Documents

Citations

The post Streptavidin, Peroxidase, R.T.U. appeared first on VectorLabs.

]]> Streptavidin, Alkaline Phosphatase https://staging.vectorlabs.com/products/streptavidin-alkaline-phosphatase-conjugated/ Fri, 28 Apr 2023 21:20:07 +0000 https://staging.vectorlabs.com/?post_type=product&p=13056 Description

Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
  • Specific covalent linkages are chosen to provide stable, highly active conjugates

Specifications

Unit Size 1 ml
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage The recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage 2-8 °C
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Alkaline Phosphatase
Format Concentrate

The post Streptavidin, Alkaline Phosphatase appeared first on VectorLabs.

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Description

Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
  • Specific covalent linkages are chosen to provide stable, highly active conjugates

Specifications

Unit Size1 ml
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageThe recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage2-8 °C
Detection Enzyme(s)Alkaline Phosphatase
ConjugateAlkaline Phosphatase
FormatConcentrate

Documents

Citations

The post Streptavidin, Alkaline Phosphatase appeared first on VectorLabs.

]]> Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) https://staging.vectorlabs.com/products/streptavidin-hrp-concentrate-elisas/ Fri, 28 Apr 2023 21:15:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=13047 Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase
  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size 1 mg
Applications Blotting Applications, Elispot, ELISAs
Recommended Usage Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 mg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

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Description

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Optimized for use in solid phase assays such as ELISAs and blotting applications
  • Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure which preserves the high specific activity of the peroxidase

  • For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

    Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

Specifications

Unit Size1 mg
ApplicationsBlotting Applications, Elispot, ELISAs
Recommended UsageDo not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. Recommended concentration range for use is 5-10 µg/ml. Note: This product is different from Cat. No. SA-5004 which has been optimized for use in immunohistochemical applications.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Citations

Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.

Vector Laboratories enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

This Horseradish peroxidase streptavidin preparation (HRP-Sav; SA-5014) is optimized for use in solid-phase assays such as ELISAs and blotting applications.

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in tissue/cell staining applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for IHC/ICC

The post Streptavidin, Peroxidase, ELISA Grade (Concentrate, for ELISAs and Blots) appeared first on VectorLabs.

]]> Streptavidin, Peroxidase (Concentrate, for IHC) https://staging.vectorlabs.com/products/streptavidin-hrp-concentrate-ihc/ Fri, 28 Apr 2023 21:02:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=13015 Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase.  Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle

 

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage Molar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Solution 0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage 2-8 °C, DO NOT FREEZE
Concentration 1.0 mg/ml active conjugate
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

The post Streptavidin, Peroxidase (Concentrate, for IHC) appeared first on VectorLabs.

]]>

Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase.  Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageMolar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Citations

The post Streptavidin, Peroxidase (Concentrate, for IHC) appeared first on VectorLabs.

]]> Alkaline Phosphatase Avidin D https://staging.vectorlabs.com/products/avidin-d-alkaline-phosphatase-labeled/ Tue, 18 Apr 2023 18:04:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=10538 Description

Alkaline Phosphatase Avidin D is produced with the highest specific activity enzymes in optimal ratios. It is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Specific covalent linkages are chosen to provide stable, highly active conjugates
  • One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8

Specifications

Unit Size 100 U
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage Reconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 0.1 mM zinc acetate, pH 7.5, 50 mg/ml Ficoll, and 2 mg/ml bovine serum albumin. Recommended concentration for use is 0.2 units/ml.
Recommended Storage 2-8 °C
Detection Enzyme(s) Alkaline Phosphatase
Conjugate Alkaline Phosphatase
Format Concentrate

The post Alkaline Phosphatase Avidin D appeared first on VectorLabs.

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Description

Alkaline Phosphatase Avidin D is produced with the highest specific activity enzymes in optimal ratios. It is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.

Features:

  • Specific covalent linkages are chosen to provide stable, highly active conjugates
  • One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8

Specifications

Unit Size100 U
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended UsageReconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 0.1 mM zinc acetate, pH 7.5, 50 mg/ml Ficoll, and 2 mg/ml bovine serum albumin. Recommended concentration for use is 0.2 units/ml.
Recommended Storage2-8 °C
Detection Enzyme(s)Alkaline Phosphatase
ConjugateAlkaline Phosphatase
FormatConcentrate

Documents

Citations

Technical Information

Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules. Specific covalent linkages are chosen to provide stable, highly active conjugates.

The post Alkaline Phosphatase Avidin D appeared first on VectorLabs.

]]> Horseradish Peroxidase Avidin D (Av-HRP), Concentrate, for ELISAs and Blots https://staging.vectorlabs.com/products/avidin-d-horseradish-peroxidase-concentrate-elisa/ Tue, 18 Apr 2023 16:42:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=10492 Description

Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

Horseradish peroxidase labeled Avidin D (Av-HRP), for ELISAs and Blots, is optimized for use in solid-phase assays such as ELISAs and blotting applications.

Specifications

Unit Size 5 mg
Applications Blotting Applications, Elispot, ELISAs
Recommended Usage For diluting this product, we recommend a buffered saline solution, approximately pH 7.5. The recommended concentration range for use is 2-5 µg/ml.
Solution 0.05 M NaHCO3, pH 8.3, preservative
Recommended Storage 2-8 °C DO NOT FREEZE
Concentration 5 mg active conjugate/ml
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

Technical Information

For HRP Avidin conjugate that is optimized for tissue/cell staining systems please see the following product:
Avidin D, Horseradish Peroxidase (Av-HRP), Concentrate, for IHC

The post Horseradish Peroxidase Avidin D (Av-HRP), Concentrate, for ELISAs and Blots appeared first on VectorLabs.

]]>

Description

Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.

Horseradish peroxidase labeled Avidin D (Av-HRP), for ELISAs and Blots, is optimized for use in solid-phase assays such as ELISAs and blotting applications.

Specifications

Unit Size5 mg
ApplicationsBlotting Applications, Elispot, ELISAs
Recommended UsageFor diluting this product, we recommend a buffered saline solution, approximately pH 7.5. The recommended concentration range for use is 2-5 µg/ml.
Solution0.05 M NaHCO3, pH 8.3, preservative
Recommended Storage2-8 °C DO NOT FREEZE
Concentration5 mg active conjugate/ml
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Technical Information

For HRP Avidin conjugate that is optimized for tissue/cell staining systems please see the following product:
 
Avidin D, Horseradish Peroxidase (Av-HRP), Concentrate, for IHC

The post Horseradish Peroxidase Avidin D (Av-HRP), Concentrate, for ELISAs and Blots appeared first on VectorLabs.

]]> Horseradish Peroxidase Avidin D (Av-HRP) https://staging.vectorlabs.com/products/avidin-d-horseradish-peroxidase-concentrate-ihc/ Tue, 11 Apr 2023 18:05:24 +0000 https://staging.vectorlabs.com/?post_type=product&p=9540 Description

Horseradish Peroxidase Avidin D (HRP Avidin D) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Horseradish Peroxidase Avidin D is offered as a concentrate (5 mg/ml) or in a ready-to-use (R.T.U.) stabilized solution (100ml,1µg/ml) in a bottle fitted with a drop dispenser top.

 

Specifications

 
Unit Size 5 mg
Applications Immunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended Usage For diluting this product, we recommend a buffered saline solution, approximately pH 7.5. The recommended concentration range for use is 2-5 µg/ml.
Solution 0.05 M NaHCO3, pH 8.2, preservative
Recommended Storage 2-8 °C DO NOT FREEZE
Concentration 5 mg active conjugate/ml
Detection Enzyme(s) Peroxidase
Conjugate Peroxidase
Format Concentrate

Technical Information

For horseradish peroxidase labeled Avidin D (Av-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Avidin D, Horseradish Peroxidase (Av-HRP), Concentrate, for ELISAs and Blots

The post Horseradish Peroxidase Avidin D (Av-HRP) appeared first on VectorLabs.

]]>

Description

Horseradish Peroxidase Avidin D (HRP Avidin D) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled Avidin D, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules.
  • Horseradish Peroxidase Avidin D is offered as a concentrate (5 mg/ml) or in a ready-to-use (R.T.U.) stabilized solution (100ml,1µg/ml) in a bottle fitted with a drop dispenser top.

Specifications

 
Unit Size5 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageFor diluting this product, we recommend a buffered saline solution, approximately pH 7.5. The recommended concentration range for use is 2-5 µg/ml.
Solution0.05 M NaHCO3, pH 8.2, preservative
Recommended Storage2-8 °C DO NOT FREEZE
Concentration5 mg active conjugate/ml
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Documents

Citations

Technical Information

For horseradish peroxidase labeled Avidin D (Av-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Avidin D, Horseradish Peroxidase (Av-HRP), Concentrate, for ELISAs and Blots

The post Horseradish Peroxidase Avidin D (Av-HRP) appeared first on VectorLabs.

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