Biotinylated Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:42 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotinylated Reagents – VectorLabs https://staging.vectorlabs.com 32 32 Protein A, Biotinylated (S. Aureus) https://staging.vectorlabs.com/products/protein-a-biotinylated-s-aureus/ Thu, 20 Apr 2023 19:25:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=11118 Description

Biotinylated Protein A (B-2001), isolated from S. aureus, is a 41 kDa protein which binds to the Fc portion of most immunoglobulins. This interaction is neither species specific nor isotype specific, as Protein A has been shown to bind IgG from a variety of species. Protein A can also interact with IgA, IgM and subclasses of IgG from different species.

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs
Recommended Usage Reconstitute by adding 0.5 ml water. The resulting solution will have the following compostition: 0.15 M NaCl, 10 mM HEPES, pH 8.0 The recommended concentration range for use is 2-5 µg/ml. For long term storage, an immunohistochemical grade, globulin-free bovine serum albumin (0.1%, w/v) may be added to the biotinylated Protein A solution prior to freezing. For short-term usage, bovine serum albumin may also be added and samples stored at 4 ºC in the presence of 0.05% sodium azide.
Recommended Storage 2-8 °C after reconstitution; -20 °C is recommended.
Conjugate Biotinylated
Format Concentrate

Technical Information

Biotinylated Protein A is useful to detect or localize immunoglobulins on cell surfaces or in tissue sections. Used with the appropriate avidin or streptavidin conjugates, immunoglobulins can be studied with brightfield, fluorescence or electron microscopy. Biotinylated Protein A can also be used to detect positive clones in monoclonal antibody production by an avidin or streptavidin enzyme conjugate.

Biotinylated Protein A is used in many systems as a substitute for a biotinylated second antibody. However, in some applications, Biotinylated Protein A may bind to endogenous immunoglobulin in the tissue producing background. In addition, Biotinylated Protein A may not bind to the species or class of primary antibody being employed. For routine immunohistochemical staining we recommend using biotinylated anti-immunoglobulins.

The post Protein A, Biotinylated (S. Aureus) appeared first on VectorLabs.

]]>

Description

Biotinylated Protein A (B-2001), isolated from S. aureus, is a 41 kDa protein which binds to the Fc portion of most immunoglobulins. This interaction is neither species specific nor isotype specific, as Protein A has been shown to bind IgG from a variety of species. Protein A can also interact with IgA, IgM and subclasses of IgG from different species.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs
Recommended UsageReconstitute by adding 0.5 ml water. The resulting solution will have the following compostition: 0.15 M NaCl, 10 mM HEPES, pH 8.0 The recommended concentration range for use is 2-5 µg/ml. For long term storage, an immunohistochemical grade, globulin-free bovine serum albumin (0.1%, w/v) may be added to the biotinylated Protein A solution prior to freezing. For short-term usage, bovine serum albumin may also be added and samples stored at 4 ºC in the presence of 0.05% sodium azide.
Recommended Storage2-8 °C after reconstitution; -20 °C is recommended.
ConjugateBiotinylated
FormatConcentrate

Documents

Citations

Technical Information

Biotinylated Protein A is useful to detect or localize immunoglobulins on cell surfaces or in tissue sections. Used with the appropriate avidin or streptavidin conjugates, immunoglobulins can be studied with brightfield, fluorescence or electron microscopy. Biotinylated Protein A can also be used to detect positive clones in monoclonal antibody production by an avidin or streptavidin enzyme conjugate.

Biotinylated Protein A is used in many systems as a substitute for a biotinylated second antibody. However, in some applications, Biotinylated Protein A may bind to endogenous immunoglobulin in the tissue producing background. In addition, Biotinylated Protein A may not bind to the species or class of primary antibody being employed. For routine immunohistochemical staining we recommend using biotinylated anti-immunoglobulins.

The post Protein A, Biotinylated (S. Aureus) appeared first on VectorLabs.

]]>