Anti-Avidin / Streptavidin Amplification Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:46 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Anti-Avidin / Streptavidin Amplification Reagents – VectorLabs https://staging.vectorlabs.com 32 32 Anti-Streptavidin Antibody, DyLight® 488 https://staging.vectorlabs.com/products/dylight-488-anti-streptavidin/ Tue, 02 May 2023 02:35:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=13944 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 1 mg active conjugate/ml
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended Usage The recommended concentration range for use is 5-20 µg/ml.
Detection Target Streptavidin
Conjugate DyLight 488
Color of Fluorescence Green
Host Species Goat
Format Concentrate

The post Anti-Streptavidin Antibody, DyLight® 488 appeared first on VectorLabs.

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

DyLight 488 Anti-Streptavidin has been optimally labeled to provide maximum fluorescence.

  • Maximum Excitation: 493 nm
  • Maximum Emission: 518 nm
  • Color: green

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration1 mg active conjugate/ml
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Detection TargetStreptavidin
ConjugateDyLight 488
Color of FluorescenceGreen
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

The capability of binding avidin or streptavidin via either biotin binding sites or through antigen binding sites, makes these biotinylated antibodies unique. These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

The post Anti-Streptavidin Antibody, DyLight® 488 appeared first on VectorLabs.

]]> Anti-Streptavidin Antibody, Unconjugated https://staging.vectorlabs.com/products/unconjugated-anti-streptavidin/ Tue, 02 May 2023 01:14:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=13826 Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications.  Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, In situ hybridization
Concentration 2 mg active IgG/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage The recommended concentration range for use is 2-5 µg/ml.
Detection Target Streptavidin
Conjugate Unconjugated
Host Species Goat
Format Concentrate

The post Anti-Streptavidin Antibody, Unconjugated appeared first on VectorLabs.

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Description

Anti-Streptavidin antibodies have been widely used as amplifying reagents in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications.  Our antibodies to streptavidin are produced in goats using our highly purified streptavidin and isolated by affinity chromatography. Anti-Streptavidin does not bind avidin and Anti-Avidin does not recognize streptavidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

More Information
Unit Size1 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration2 mg active IgG/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended UsageThe recommended concentration range for use is 2-5 µg/ml.
Detection TargetStreptavidin
ConjugateUnconjugated
Host SpeciesGoat
FormatConcentrate

Documents

 

Citations

Technical Information

These affinity purified antibodies are also available biotin or fluorescein-labeled.

The post Anti-Streptavidin Antibody, Unconjugated appeared first on VectorLabs.

]]> Goat Anti-Streptavidin Antibody, Biotinylated https://staging.vectorlabs.com/products/anti-streptavidin-antibody-biotinylated/ Mon, 24 Apr 2023 15:26:31 +0000 https://staging.vectorlabs.com/?post_type=product&p=11156 Description

Biotinylated Anti-Streptavidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding streptavidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Anti-Streptavidin does not bind avidin, and Anti-Avidin does not recognize streptavidin.

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Concentration 0.5 mg active conjugate/ml
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage The recommended concentration range for use is 1-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection Target Streptavidin
Conjugate Biotinylated
Host Species Goat
Format Concentrate

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figures illustrate different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 Jpg B2-fig1-3-800 Vectorlabs

The post Goat Anti-Streptavidin Antibody, Biotinylated appeared first on VectorLabs.

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Description

Biotinylated Anti-Streptavidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding streptavidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Anti-Streptavidin does not bind avidin, and Anti-Avidin does not recognize streptavidin. 

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Concentration0.5 mg active conjugate/ml
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended UsageThe recommended concentration range for use is 1-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection TargetStreptavidin
ConjugateBiotinylated
Host SpeciesGoat
FormatConcentrate

Documents

Citations

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figures illustrate different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 jpg

The post Goat Anti-Streptavidin Antibody, Biotinylated appeared first on VectorLabs.

]]> Goat Anti-Avidin D Antibody, Biotinylated https://staging.vectorlabs.com/products/biotinylated-anti-avidin/ Mon, 24 Apr 2023 15:18:23 +0000 https://staging.vectorlabs.com/?post_type=product&p=11143 Description

Biotinylated Anti-Avidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding avidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography. Anti-Avidin does not bind streptavidin and Anti-Streptavidin does not recognize avidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

Unit Size 0.5 mg
Applications Immunofluorescence, In situ hybridization
Recommended Storage 2-8 °C; Store frozen for long term storage
Recommended Usage Reconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 1-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection Target Avidin
Conjugate Biotinylated
Host Species Goat
Format Lyophilized

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figure illustrates different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 Jpg B2-fig1-3-800 Vectorlabs

The post Goat Anti-Avidin D Antibody, Biotinylated appeared first on VectorLabs.

]]>

Description

Biotinylated Anti-Avidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding avidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Our antibodies to avidin are produced in goats using our highly purified avidin and isolated by affinity chromatography. Anti-Avidin does not bind streptavidin and Anti-Streptavidin does not recognize avidin. These antibodies provide opportunities to significantly amplify signals in many applications.

Specifications

Unit Size0.5 mg
ApplicationsImmunofluorescence, In situ hybridization
Recommended Storage2-8 °C; Store frozen for long term storage
Recommended UsageReconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1 mg/ml bovine serum albumin. The recommended concentration range for use is 1-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection TargetAvidin
ConjugateBiotinylated
Host SpeciesGoat
FormatLyophilized

Documents

Citations

Technical Information

These antibodies can be used either as part of preformed complexes or in sequence to amplify fluorescent signals. When used in sequence, the target is first labeled with fluorochrome-conjugated avidin or streptavidin, followed by incubation with Biotinylated Anti-Avidin or Biotinylated Anti-Streptavidin, followed by a second layer of fluorochrome-conjugated avidin or streptavidin. This sequence can be repeated. This multi-layered approach introduces more fluorochromes at the target site and can provide a multi-fold amplification over a single layer.

These affinity purified antibodies are also available unconjugated or fluorescein-labeled.

The following figure illustrates different experimental setups used to achieve increasing levels of sensitivity.

B2 Fig1 3 800 jpg

The post Goat Anti-Avidin D Antibody, Biotinylated appeared first on VectorLabs.

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