Thiol Reactive – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:47 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Thiol Reactive – VectorLabs https://staging.vectorlabs.com 32 32 Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ https://staging.vectorlabs.com/products/bis-mal-lysine-dpeg4-dpeg12-trism-dpeg243/ Sat, 24 Feb 2024 02:17:18 +0000 https://staging.vectorlabs.com/?post_type=product&p=39804 Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(m-dPEG®24)3, product number QBD-11633, is a unique product from Vector Laboratories. It consists of a carboxylic-acid terminated, branched dPEG® construct built around a tris core and functionalized with a short dPEG® linker that contains two thiol-reactive maleimide groups attached to lysine. The dPEG® spacers are 152 and 156 atoms, with average lengths of 113.0 and 115.4 Å. Three uncharged methoxy-terminated dPEG® spacers branch from the tris core of this product. Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(m-dPEG®24)3 is designed to modify the pharmacodynamics (PD), pharmacokinetics (PK), and biodistribution (BD) of conjugated biomolecules, while the bis-maleimide moiety is designed to bridge disulfide bonds by forming stable thioether bonds through the thiol-maleimide reaction.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. The two maleimide groups can bridge a reduced disulfide bond through the thiol-maleimide reaction, forming stable thioether bonds. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates.

Vector Laboratories' dPEG® technology provides high-quality, single molecular weight, discrete chain-length PEG (dPEG®) products for bioconjugation in therapeutic, diagnostic, and clinical research applications. Unlike traditional, non-uniform polymer PEGs, dPEG® products are synthesized in stepwise fashion from high-purity starting materials. The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated. Moreover, optimization of the conjugate molecule's BD, PK, and PD is possible.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 4825.69; single compound;
Chemical formula C₂₁₈H₄₁₆N₁₀O₁₀₃
CAS N/A
Purity > 95%
Spacers dPEG® Spacers are 149 and 153 atoms; 110.3 and 112.5 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ appeared first on VectorLabs.

]]>

Description

Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(m-dPEG®24)3, product number QBD-11633, is a unique product from Vector Laboratories. It consists of a carboxylic-acid terminated, branched dPEG® construct built around a tris core and functionalized with a short dPEG® linker that contains two thiol-reactive maleimide groups attached to lysine. The dPEG® spacers are 152 and 156 atoms, with average lengths of 113.0 and 115.4 Å. Three uncharged methoxy-terminated dPEG® spacers branch from the tris core of this product. Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(m-dPEG®24)3 is designed to modify the pharmacodynamics (PD), pharmacokinetics (PK), and biodistribution (BD) of conjugated biomolecules, while the bis-maleimide moiety is designed to bridge disulfide bonds by forming stable thioether bonds through the thiol-maleimide reaction.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. The two maleimide groups can bridge a reduced disulfide bond through the thiol-maleimide reaction, forming stable thioether bonds. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates.

Vector Laboratories’ dPEG® technology provides high-quality, single molecular weight, discrete chain-length PEG (dPEG®) products for bioconjugation in therapeutic, diagnostic, and clinical research applications. Unlike traditional, non-uniform polymer PEGs, dPEG® products are synthesized in stepwise fashion from high-purity starting materials. The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated. Moreover, optimization of the conjugate molecule’s BD, PK, and PD is possible.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight4825.69; single compound;
Chemical formulaC₂₁₈H₄₁₆N₁₀O₁₀₃
CASN/A
Purity> 95%
SpacersdPEG® Spacers are 149 and 153 atoms; 110.3 and 112.5 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ appeared first on VectorLabs.

]]> Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ https://staging.vectorlabs.com/products/bis-mal-lysine-dpeg4-dpeg12-tris-dpeg24-acid3/ Sat, 24 Feb 2024 02:17:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=39796 Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(-dPEG®24 acid)3, product number QBD-11630, is a unique product from Vector Laboratories. It consists of a carboxylic-acid terminated, branched dPEG® construct built around a tris core and functionalized with a short dPEG® linker that contains two thiol-reactive maleimide groups attached to lysine. The dPEG® spacers are 152 and 156 atoms, with average lengths of 113.0 and 115.4 Å. The tris-core branched portion of this product is designed to modify the pharmacodynamics (PD), pharmacokinetics (PK), and biodistribution (BD) of conjugated biomolecules, while the bis-maleimide moiety is designed to bridge disulfide bonds by forming stable thioether bonds through the thiol-maleimide reaction.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. The two maleimide groups can bridge a reduced disulfide bond through the thiol-maleimide reaction, forming stable thioether bonds. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates. Users should note that the free carboxylates on the three long arms of the molecule may alter the charge of conjugates formed with Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(-dPEG®24 acid)3.

Vector Laboratories' dPEG® technology provides high-quality, single molecular weight, discrete chain-length PEG (dPEG®) products for bioconjugation in therapeutic, diagnostic, and clinical research applications. Unlike traditional, non-uniform polymer PEGs, dPEG® products are synthesized in stepwise fashion from high-purity starting materials. The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated. Moreover, optimization of the conjugate molecule's BD, PK, and PD is possible.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 4999.80; single compound;
Chemical formula C₂₂₄H₄₂₂N₁₀O₁₀₉
CAS N/A
Purity > 95%
Spacers dPEG® Spacers are 152 and 156 atoms; 113 and 115.4 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ appeared first on VectorLabs.

]]>

Description

Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(-dPEG®24 acid)3, product number QBD-11630, is a unique product from Vector Laboratories. It consists of a carboxylic-acid terminated, branched dPEG® construct built around a tris core and functionalized with a short dPEG® linker that contains two thiol-reactive maleimide groups attached to lysine. The dPEG® spacers are 152 and 156 atoms, with average lengths of 113.0 and 115.4 Å. The tris-core branched portion of this product is designed to modify the pharmacodynamics (PD), pharmacokinetics (PK), and biodistribution (BD) of conjugated biomolecules, while the bis-maleimide moiety is designed to bridge disulfide bonds by forming stable thioether bonds through the thiol-maleimide reaction.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. The two maleimide groups can bridge a reduced disulfide bond through the thiol-maleimide reaction, forming stable thioether bonds. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates. Users should note that the free carboxylates on the three long arms of the molecule may alter the charge of conjugates formed with Bis-MAL-Lysine-dPEG®4-dPEG®12-Tris(-dPEG®24 acid)3.

Vector Laboratories’ dPEG® technology provides high-quality, single molecular weight, discrete chain-length PEG (dPEG®) products for bioconjugation in therapeutic, diagnostic, and clinical research applications. Unlike traditional, non-uniform polymer PEGs, dPEG® products are synthesized in stepwise fashion from high-purity starting materials. The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated. Moreover, optimization of the conjugate molecule’s BD, PK, and PD is possible.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight4999.80; single compound;
Chemical formulaC₂₂₄H₄₂₂N₁₀O₁₀₉
CASN/A
Purity> 95%
SpacersdPEG® Spacers are 152 and 156 atoms; 113 and 115.4 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

 

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-MAL-Lysine-dPEG®₄-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₂₄-Tris(-dPEG®₂₄-Tris (m-dPEG®₂₄)₃)₃ https://staging.vectorlabs.com/products/mal-dpeg24-tris-dpeg24-tris-m-dpeg2433/ Sat, 24 Feb 2024 02:17:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=39706 MAL-dPEG®24-Tris(-dPEG®24-Tris (m-dPEG®24)3)3, product number QBD-11487, is a nine-branched, 15.4 kDa, sulfhydryl-reactive, monodispersed PEGylation reagent built around tris cores from high-purity, single molecular weight, discrete PEG (dPEG®) compounds. The attachment point is a thiol-reactive maleimide group. Nine uncharged methoxy-terminated dPEG®24 linkers arising from four tris cores in a 1:3 arrangement form the branches. The average dPEG® spacer length from the maleimide ring attachment point to the terminal methoxy groups is 247 atoms (158.8 Å). This product is designed for biomolecular conjugation to modulate the pharmacokinetics (PK) and biodistribution (BD) of biomolecules such as antibodies or other proteins. It may also be used to passivate surfaces to prevent non-specific interactions.

Vector Laboratories' dPEG® technology uses high-purity, single molecular-weight PEG linkers and spacers with discrete chain lengths. In contrast, traditional, non-uniform polymer PEG linkers and spacers have an intractable mixture of different chain lengths of PEG, where each length of PEG in the mix has a unique molecular weight. Analyzing dPEG® products and conjugates made with them is far more straightforward than analyzing non-uniform PEG products and their conjugates.

The terminal maleimide group in MAL-dPEG®24-Tris(-dPEG®24-Tris(m-dPEG®24)3)3, product number QBD-11487, provides the sole attachment point for the dPEG® construct to join a free thiol (sulfhydryl) group, forming a stable thioether bond. Surface-accessible, free thiols are uncommon in biomolecules but may be preferred over surface-accessible amines for PEGylation of biomolecules. Free thiols can also be exposed by creating antibody fragments or reduction reactions targeting disulfide bonds.

PEGylation of biomolecules with MAL-dPEG®24-Tris(-dPEG®24-Tris(m-dPEG®24)3)3 greatly increases a conjugate molecule's hydrodynamic volume and water solubility and shields the conjugate from opsonization by the immune system. These effects prevent renal clearance of the conjugate by the body and reduce or eliminate opsonization, extending the conjugate's serum half-life. The longer serum half-life can lead to lower dosing requirements for the PEGylated conjugate compared to the non-PEGylated conjugate while maintaining diagnostic or therapeutic efficacy.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 15592.45; single compund;
Chemical formula C₇₀₄H₁₃₈₆N₁₈O₃₄₃
CAS N/A
Purity > 95%
Spacers dPEG® Spacer is 247 atoms and 158.8 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMAC or DMSO
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₂₄-Tris(-dPEG®₂₄-Tris (m-dPEG®₂₄)₃)₃ appeared first on VectorLabs.

]]>

Description

MAL-dPEG®24-Tris(-dPEG®24-Tris (m-dPEG®24)3)3, product number QBD-11487, is a nine-branched, 15.4 kDa, sulfhydryl-reactive, monodispersed PEGylation reagent built around tris cores from high-purity, single molecular weight, discrete PEG (dPEG®) compounds. The attachment point is a thiol-reactive maleimide group. Nine uncharged methoxy-terminated dPEG®24 linkers arising from four tris cores in a 1:3 arrangement form the branches. The average dPEG® spacer length from the maleimide ring attachment point to the terminal methoxy groups is 247 atoms (158.8 Å). This product is designed for biomolecular conjugation to modulate the pharmacokinetics (PK) and biodistribution (BD) of biomolecules such as antibodies or other proteins. It may also be used to passivate surfaces to prevent non-specific interactions.

Vector Laboratories’ dPEG® technology uses high-purity, single molecular-weight PEG linkers and spacers with discrete chain lengths. In contrast, traditional, non-uniform polymer PEG linkers and spacers have an intractable mixture of different chain lengths of PEG, where each length of PEG in the mix has a unique molecular weight. Analyzing dPEG® products and conjugates made with them is far more straightforward than analyzing non-uniform PEG products and their conjugates.

The terminal maleimide group in MAL-dPEG®24-Tris(-dPEG®24-Tris(m-dPEG®24)3)3, product number QBD-11487, provides the sole attachment point for the dPEG® construct to join a free thiol (sulfhydryl) group, forming a stable thioether bond. Surface-accessible, free thiols are uncommon in biomolecules but may be preferred over surface-accessible amines for PEGylation of biomolecules. Free thiols can also be exposed by creating antibody fragments or reduction reactions targeting disulfide bonds.

PEGylation of biomolecules with MAL-dPEG®24-Tris(-dPEG®24-Tris(m-dPEG®24)3)3 greatly increases a conjugate molecule’s hydrodynamic volume and water solubility and shields the conjugate from opsonization by the immune system. These effects prevent renal clearance of the conjugate by the body and reduce or eliminate opsonization, extending the conjugate’s serum half-life. The longer serum half-life can lead to lower dosing requirements for the PEGylated conjugate compared to the non-PEGylated conjugate while maintaining diagnostic or therapeutic efficacy.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight15592.45; single compund;
Chemical formulaC₇₀₄H₁₃₈₆N₁₈O₃₄₃
CASN/A
Purity> 95%
SpacersdPEG® Spacer is 247 atoms and 158.8 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMAC or DMSO
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post MAL-dPEG®₂₄-Tris(-dPEG®₂₄-Tris (m-dPEG®₂₄)₃)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ https://staging.vectorlabs.com/products/mal-dpeg12-trism-dpeg243/ Sat, 24 Feb 2024 02:17:02 +0000 https://staging.vectorlabs.com/?post_type=product&p=39603 MAL-dPEG®12-Tris(m-dPEG®24)3, product number QBD-11471, is a thiol-reactive, methyl-terminated, four-arm monodispersed PEG product designed to modulate biodistribution of conjugates. Tris forms the core of the molecule. Three equal-length methyl-terminated long arms plus one maleimido-terminated short arm extend from the tris core.

The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated.

The maleimidopropyl (MAL) group reacts chemoselectively with free thiols at pH 6.5 - 7.5 to form stable thioether bonds. Maleimide-thiol conjugations are click chemistry-like reactions and one of the most popular ways to form thioether bonds in biomolecules.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 4299.06; single compound;
Chemical formula C₁₉₄H₃₇₈N₆O₉₄
CAS N/A
Purity > 95%
Spacers dPEG® Spacer is 127 atoms and 103.0 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ appeared first on VectorLabs.

]]>

Description

MAL-dPEG®12-Tris(m-dPEG®24)3, product number QBD-11471, is a thiol-reactive, methyl-terminated, four-arm monodispersed PEG product designed to modulate biodistribution of conjugates. Tris forms the core of the molecule. Three equal-length methyl-terminated long arms plus one maleimido-terminated short arm extend from the tris core.

The amphiphilic dPEG® construct imparts hydrophilicity to conjugates, reduces or eliminates the conjugates’ antigenicity, and increases the hydrodynamic volume of conjugated molecules. With increased hydrodynamic volume, the renal clearance of conjugate molecules is reduced or eliminated.

The maleimidopropyl (MAL) group reacts chemoselectively with free thiols at pH 6.5 – 7.5 to form stable thioether bonds. Maleimide-thiol conjugations are click chemistry-like reactions and one of the most popular ways to form thioether bonds in biomolecules.

This product is designed for modifying biomolecules like antibodies and antibody fragments but can be used in any bioconjugate application where free thiols are available for reaction. Potential product applications include reducing or eradicating renal clearance, extending in vivo circulating half-life, and suppressing immune responses to conjugates.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight4299.06; single compound;
Chemical formulaC₁₉₄H₃₇₈N₆O₉₄
CASN/A
Purity> 95%
SpacersdPEG® Spacer is 127 atoms and 103.0 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Site specific discrete PEGylation of 124I-labeled mCC49 Fab’ fragments improves tumor microPET/CT imaging in mice. Haiming Ding, Michelle M. Carleton, Stephen P. Povoski, Keisha Milum, Krishan Kumar, Kothandaraman Shankaran, George H. Hinkle, David Colcher, Rich Brody, Paul D. Davis, Alex Pokora, Mitchell Phelps, Edward W. Martin Jr., and Michael F. Tweedle. Bioconjugate Chemistry. 2013, 24 (11) pp 1945-1954. October 4, 2013. DOI: 10.1021/bc400375f.

Tumor uptake of pegylated diabodies: Balancing systemic clearance and vascular transport. Qing Li, Jason B. White, Norman C. Peterson, Keith W. Rickert, Christopher O. Lloyd, Kevin L. Allen, Kim Rosenthal, Xizhe Gao, Herren Wu, William F. Dall’Acqua, M. Jack Borrok, and Ping Tsui. Journal of Controlled Release. 2018, 279 pp 126-135. April 6, 2018. DOI: 10.1016/j.jconrel.2018.04.013.

The post MAL-dPEG®₁₂-Tris(m-dPEG®₂₄)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ https://staging.vectorlabs.com/products/mal-dpeg12-tris-dpeg24-acid3/ Sat, 24 Feb 2024 02:17:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=39595 MAL-dPEG®12-Tris(-dPEG®24-acid)3, product number QBD-11451, is a thiol- and amine-reactive, four-arm, monodispersed PEG with one arm shorter than the other three. MAL-dPEG®12-Tris(-dPEG®24-acid)3 is a monodispersed branched PEG compound built around a tris core. From the maleimide olefin to carbonyl carbon immediately before the tris core is 46 atoms. From the tris core to the carbonyl carbon on each branch is 83 atoms. The total linker length is 129 atoms. The maleimido group that terminates the short arm of this product reacts with sulfhydryl groups to form thioether bonds. It conjugates directly to target biomolecules.

This product is useful for charge modulation and elimination of renal clearance in drug delivery and imaging applications, among others. The dPEG® spacer length is from the maleimide olefin to the carbonyl carbon on each of the three long arms. The terminal carboxylic acid groups can be modified with various functional or reactive groups (e.g., NHS, TFP esters) or used as-is for charge modulation. In a peer-reviewed scientific publication, this product was shown to alter the biodistribution of the conjugate when used as a charge modulator on the antibody CC49, leading to improved microPET/CT imaging in mice.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 4473.17; single compound;
Chemical formula C₂₀₀H₃₈₄N₆O₁₀₀
CAS N/A
Purity > 95%
Spacers dPEG® Spacer is 130 atoms and 102.4 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO, water or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ appeared first on VectorLabs.

]]>

Description

MAL-dPEG®12-Tris(-dPEG®24-acid)3, product number QBD-11451, is a thiol- and amine-reactive, four-arm, monodispersed PEG with one arm shorter than the other three. MAL-dPEG®12-Tris(-dPEG®24-acid)3 is a monodispersed branched PEG compound built around a tris core. From the maleimide olefin to carbonyl carbon immediately before the tris core is 46 atoms. From the tris core to the carbonyl carbon on each branch is 83 atoms. The total linker length is 129 atoms. The maleimido group that terminates the short arm of this product reacts with sulfhydryl groups to form thioether bonds. It conjugates directly to target biomolecules.

This product is useful for charge modulation and elimination of renal clearance in drug delivery and imaging applications, among others. The dPEG® spacer length is from the maleimide olefin to the carbonyl carbon on each of the three long arms. The terminal carboxylic acid groups can be modified with various functional or reactive groups (e.g., NHS, TFP esters) or used as-is for charge modulation. In a peer-reviewed scientific publication, this product was shown to alter the biodistribution of the conjugate when used as a charge modulator on the antibody CC49, leading to improved microPET/CT imaging in mice.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight4473.17; single compound;
Chemical formulaC₂₀₀H₃₈₄N₆O₁₀₀
CASN/A
Purity> 95%
SpacersdPEG® Spacer is 130 atoms and 102.4 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO, water or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Site specific discrete PEGylation of 124I-labeled mCC49 Fab’ fragments improves tumor microPET/CT imaging in mice. Haiming Ding, Michelle M Carleton, Stephen P Povoski, Keisha Milum, Krishan Kumar, Kothandaraman Shankaran, George H Hinkle, David Colcher, Rich Brody, Paul D Davis, Alexander Pokora, Mitchell Phelps, Edward W. Martin, and Michael F. Tweedle. Bioconjugate Chemistry. 2013, October 4, 2013. DOI: 10.1021/bc400375f.

The post MAL-dPEG®₁₂-Tris(-dPEG®₂₄-acid)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₄-(m-dPEG®₂₄)₃ https://staging.vectorlabs.com/products/mal-dpeg4-m-dpeg243/ Sat, 24 Feb 2024 02:10:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=36187 MAL-dPEG®4-(m-dPEG®24)3, product number QBD-10456, is a sulfhydryl-reactive, branched polyethylene glycol (PEG) product built around a tris core that is useful for modifying surfaces and biomolecules containing free thiols. Vector Laboratories, Inc. manufactures this monodispersed PEG product with a single molecular weight and discrete chain length and sells it under the dPEG® tradename. MAL-dPEG®4-(m-dPEG®24)3 increases the hydrophilicity of conjugated molecules; reduces non-specific, hydrophobic interactions; and decreases or eliminates the antigenicity of conjugates. Also, because it is a single molecule, the analysis of conjugates made with MAL-dPEG®4-(m-dPEG®24)3 is simplified compared to the analysis of conjugates prepared with traditional dispersed PEG products.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 3946.64; single compound
Chemical formula C₁₇₈H₃₄₆N₆O₈₆
CAS 1334178-05-0
Purity > 96%
Spacers dPEG® Spacer is 103 atoms and 108.3 Å, Avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₄-(m-dPEG®₂₄)₃ appeared first on VectorLabs.

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Description

MAL-dPEG®4-(m-dPEG®24)3, product number QBD-10456, is a sulfhydryl-reactive, branched polyethylene glycol (PEG) product built around a tris core that is useful for modifying surfaces and biomolecules containing free thiols. Vector Laboratories, Inc. manufactures this monodispersed PEG product with a single molecular weight and discrete chain length and sells it under the dPEG® tradename. MAL-dPEG®4-(m-dPEG®24)3 increases the hydrophilicity of conjugated molecules; reduces non-specific, hydrophobic interactions; and decreases or eliminates the antigenicity of conjugates. Also, because it is a single molecule, the analysis of conjugates made with MAL-dPEG®4-(m-dPEG®24)3 is simplified compared to the analysis of conjugates prepared with traditional dispersed PEG products.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight3946.64; single compound
Chemical formulaC₁₇₈H₃₄₆N₆O₈₆
CAS1334178-05-0
Purity> 96%
SpacersdPEG® Spacer is 103 atoms and 108.3 Å, Avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Chapter 1, p. 30.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post MAL-dPEG®₄-(m-dPEG®₂₄)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₄-(m-dPEG®₄)₃ https://staging.vectorlabs.com/products/mal-dpeg4-m-dpeg43/ Sat, 24 Feb 2024 02:10:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=36171 MAL-dPEG®4-(m-dPEG®4)3, product number QBD-10416, is a sulfhydryl-reactive, branched polyethylene glycol (PEG) product built around a tris core that is designed to modify surfaces and biomolecules containing free thiols. Vector Laboratories, Inc. manufactures this monodispersed PEG product with a single molecular weight and discrete chain length and sells it under the dPEG® tradename. MAL-dPEG®4-(m-dPEG®4)3 increases the hydrophilicity of conjugated molecules; reduces non-specific, hydrophobic interactions; and decreases or eliminates the antigenicity of conjugates. Also, because it is a single molecule, the analysis of conjugates made with MAL-dPEG®4-(m-dPEG®4)3 is simplified compared to the analysis of conjugates prepared with traditional dispersed PEG products. The maleimide reactive group reacts rapidly and selectively with free thiols (i.e., sulfhydryl groups) in aqueous media at pH 6.5 - 7.5. The maleimide-thiol reaction is a type of "click chemistry." However, above pH 7.5, maleimide groups react with free amines in addition to free thiols.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1303.49; single compound
Chemical formula C₅₈H₁₀₆N₆O₂₆
CAS 1333154-72-5
Purity > 96%
Spacers dPEG® Spacer is 43 atoms and 47.9 Å, Avg
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₄-(m-dPEG®₄)₃ appeared first on VectorLabs.

]]>

Description

MAL-dPEG®4-(m-dPEG®4)3, product number QBD-10416, is a sulfhydryl-reactive, branched polyethylene glycol (PEG) product built around a tris core that is designed to modify surfaces and biomolecules containing free thiols. Vector Laboratories, Inc. manufactures this monodispersed PEG product with a single molecular weight and discrete chain length and sells it under the dPEG® tradename. MAL-dPEG®4-(m-dPEG®4)3 increases the hydrophilicity of conjugated molecules; reduces non-specific, hydrophobic interactions; and decreases or eliminates the antigenicity of conjugates. Also, because it is a single molecule, the analysis of conjugates made with MAL-dPEG®4-(m-dPEG®4)3 is simplified compared to the analysis of conjugates prepared with traditional dispersed PEG products. The maleimide reactive group reacts rapidly and selectively with free thiols (i.e., sulfhydryl groups) in aqueous media at pH 6.5 – 7.5. The maleimide-thiol reaction is a type of “click chemistry.” However, above pH 7.5, maleimide groups react with free amines in addition to free thiols.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight1303.49; single compound
Chemical formulaC₅₈H₁₀₆N₆O₂₆
CAS1333154-72-5
Purity> 96%
SpacersdPEG® Spacer is 43 atoms and 47.9 Å, Avg
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Chapter 1, p. 30.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post MAL-dPEG®₄-(m-dPEG®₄)₃ appeared first on VectorLabs.

]]> MAL-dPEG®₄-(m-dPEG®₁₂)₃ https://staging.vectorlabs.com/products/mal-dpeg4-m-dpeg123/ Sat, 24 Feb 2024 02:10:24 +0000 https://staging.vectorlabs.com/?post_type=product&p=36163 MAL-dPEG®4-(m-dPEG®12)3, QBD-10406, is a thiol-reactive, branched, monodisperse PEG product that can modify the performance and distribution of biologic drugs. This single molecular weight, discrete-length PEG (dPEG®) product consists of a tris core from which branch three methyl-terminated dPEG®12 arms and one maleimide-functionalized dPEG®4 arm. From the reactive site on the maleimido group to the terminal methyl moiety on each of the dPEG®12 branches, the dPEG® spacer is 67 atoms (75.8 Å). This unique branched dPEG® construct increases the water solubility and hydrodynamic volume of conjugates that incorporate it. Also, MAL-dPEG®4-(m-dPEG®12)3 can reduce or eliminate immunogenicity and non-specific, hydrophobic interactions.

MAL-dPEG®4-(m-dPEG®12)3 is best used to modify proteins, peptides, and other large biomolecules. Maleimide reacts with free thiols that occur in such molecules. The thiol-maleimide reaction is rapid and specific within the optimal pH range (6.5 – 7.5).
Scientific publications report using MAL-dPEG®4-(m-dPEG®12)3 several times. These uses include probing protein structure and function, PEGylating cell surfaces, and dissecting protein-protein interactions. The reference work Bioconjugate Techniques, 3rd edition, by Greg T. Hermanson, contains specific protocols for the maleimide-thiol reaction.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 2360.75
Chemical formula C₁₀₆H₂₀₂N₆O₅₀
CAS 1334178-05-0
Purity > 96%
Spacers dPEG® Spacer is 67 atoms and 75.8 Å, Avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO, water or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post MAL-dPEG®₄-(m-dPEG®₁₂)₃ appeared first on VectorLabs.

]]>

Description

MAL-dPEG®4-(m-dPEG®12)3, QBD-10406, is a thiol-reactive, branched, monodisperse PEG product that can modify the performance and distribution of biologic drugs. This single molecular weight, discrete-length PEG (dPEG®) product consists of a tris core from which branch three methyl-terminated dPEG®12 arms and one maleimide-functionalized dPEG®4 arm. From the reactive site on the maleimido group to the terminal methyl moiety on each of the dPEG®12 branches, the dPEG® spacer is 67 atoms (75.8 Å). This unique branched dPEG® construct increases the water solubility and hydrodynamic volume of conjugates that incorporate it. Also, MAL-dPEG®4-(m-dPEG®12)3 can reduce or eliminate immunogenicity and non-specific, hydrophobic interactions.

MAL-dPEG®4-(m-dPEG®12)3 is best used to modify proteins, peptides, and other large biomolecules. Maleimide reacts with free thiols that occur in such molecules. The thiol-maleimide reaction is rapid and specific within the optimal pH range (6.5 – 7.5).
Scientific publications report using MAL-dPEG®4-(m-dPEG®12)3 several times. These uses include probing protein structure and function, PEGylating cell surfaces, and dissecting protein-protein interactions. The reference work Bioconjugate Techniques, 3rd edition, by Greg T. Hermanson, contains specific protocols for the maleimide-thiol reaction.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight2360.75
Chemical formulaC₁₀₆H₂₀₂N₆O₅₀
CAS1334178-05-0
Purity> 96%
SpacersdPEG® Spacer is 67 atoms and 75.8 Å, Avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO, water or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Chapter 1, p. 30.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Enzymatic Deglutathionylation to Generate Interleukin-4 Cysteine Muteins with Free Thiol. Viswanadham Duppatla, Maja Gjorgjevikj, Werner Schmitz, Mathias Kottmair, Thomas D. Mueller, and Walter Sebald. Bioconjugate Chem., 2012, 23 (7), pp 1396–1405 June 9, 2012. DOI: 10.1021/bc2004389.

Inactivation by oxidation and recruitment into stress granules of hOGG1 but not APE1 in human cells exposed to sub-lethal concentrations of cadmium. Anne Bravard, Anna Campalans, Monique Vacher, Barbara Gouget, Céline Levalois, Sylvie Chevillard, J. Pablo Radicella. Mutation Research. 2010, 685 (1-2) pp 61-69. October 2, 2009. DOI: 10.1016/j.mrfmmm.2009.09.013.

Surface Location of Individual Residues of SlpA Provides Insight into the Lactobacillus brevis S-Layer. Heikki Vilen, Ulla Hynönen, Helga Badelt-Lichtblau, Nicola Ilk, Pentti Jääskeläinen, Mika Torkkeli and Airi Palva. J. Bacteriol. 2009, 191 (10) pp 3339-3351. March 20, 2009. DOI: 10.1128/JB.01782-08.

Dissecting Molecular Interactions Involved in Recognition of Target Disulfides by the Barley Thioredoxin System. Olof Bjornberg, Kenji Maeda, Birte Svensson, and Per Hagglund. Biochemistry. 2012, 51 (49) pp 9930-9939. November 19, 2012. DOI: 10.1021/bi301051b.

The Chloroplast Twin Arginine Transport (Tat) Component, Tha4, Undergoes Conformational Changes Leading to Tat Protein Transport. Cassie Aldridge, Amanda Storm, Kenneth Cline and Carole Dabney-Smith. J. Biol. Chem. 2012, 287 (1) pp 34752-34763. August 15, 2012. DOI: 10.1074/jbc.M112.385666.

Inhibition of fatty acid oxidation enhances oxidative protein folding and protects hepatocytes from endoplasmic reticulum stress. Heather M. Tyra, Douglas R. Spitz, and D. Thomas Rutkowski. Molecular Biology of the Cell. 2012, 23 (5) pp 811-819. March 1, 2012. DOI: 10.1091/mbc.E11-12-1011.

Oxidation Status of Human OGG1-S326C Polymorphic Variant Determines Cellular DNA Repair Capacity. Anne Bravard, Monique Vacher, Eva Moritz,Laurence Vaslin, Janet Hall,Bernd Epe,and J. Pablo Radicella. Cancer Research.2009, 69 (8) pp 3642-3649.April 7, 2009. DOI: 10.1158/0008-5472.CAN-08-3943.

Tau protein assembles into isoform- and disulfide-dependent polymorphic fibrils with distinct structural properties. Furukawa Y, Kaneko K, Nukina N. . The Journal of Biological Chemistry. 2011, 286 (31) pp 27236-46. June 9, 2011. DOI: 10.1074/jbc.M111.248963.

Type IV Pilus Secretins Have Extracellular C Termini. Joshua A. Lieberman, Courtney D. Petro, Stefani Thomas, Austin Yang and Michael S. Donnenberga. mBio. 2015, 6(2) pp 1-13. March 24, 2015. DOI: 10.1128/mBio.00322-15.

The Regulatory Domain of Squalene Monooxygenase Contains a Re-entrant Loop and Senses Cholesterol via a Conformational Change. Vicky Howe, Ngee Kiat Chua, Julian Stevenson, and Andrew J. Brown. The Journal of Biological Chemistry. 2015, 290 (42). October 3, 2015. DOI: 10.1074/jbc.M115.675181.

Development of Raman Spectroscopy as a clinical Diagnostic Tool. Santa Borel. Tspace. 2016, pp 1-99. November 2016. http://hdl.handle.net/1807/74538.

The post MAL-dPEG®₄-(m-dPEG®₁₂)₃ appeared first on VectorLabs.

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