Amine Reactive – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:23 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Amine Reactive – VectorLabs https://staging.vectorlabs.com 32 32 TFP-dPEG®₄-(m-dPEG®₁₁)₃-ester https://staging.vectorlabs.com/products/tfp-dpeg4-m-dpeg113-ester/ Sat, 24 Feb 2024 02:16:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=39542 TFP-dPEG®4-(m-dPEG®11)3 ester, product number QBD-11401, is a water-soluble, amine-reactive, discrete PEG (dPEG®) PEGylation reagent built. This product is built around a tris core and consists of a short (21 atoms) amine-reactive 2,3,5,6-tetrafluorophenyl (TFP) ester ""tail"" on a uniform (i.e., monodispersed) PEG4 tail and three (3) medium-length (35 atoms) branches composed from methyl-terminated dPEG®11 (m-dPEG®11). The total dPEG® spacer length from the reactive end to the methyl termini is 63 atoms (74.1 Å average). TFP-dPEG®4-(m-dPEG®11)3 ester is used to PEGylate and modify amine-reactive biomolecules and surfaces. This hydrophilic branched dPEG® product greatly increases the conjugates' hydrodynamic volumes, reducing immunogenicity and renal clearance. Moreover, this product reduces or eliminates non-specific binding to conjugates and surfaces.

TFP esters react with free amines like NHS esters to form stable amide bonds. TFP esters' optimal reaction pH is 7.5 - 8.0, which is higher than NHS esters (optimal pH range 7.0 - 7.5). However, published research has shown that, within the optimal reaction pH range, TFP esters are more reactive toward amines than NHS esters are within their optimal pH range. Applications for TFP-dPEG®4-(m-dPEG®11)3 ester, product number QBD-11401, include the following:
conjugating proteins and other biomolecules to modify PK and BD;
PEGylating amine-coated surfaces (e.g., nanoparticles) to sterically shield the surfaces and reduce non-specific, hydrophobic interactions;
PEGylating amine-coated organic or inorganic surfaces (e.g., carbon, gold, silver nanoparticles) to reduce or eliminate immunogenicity;
PEGylating biomolecules to reduce renal clearance and increase serum half-life; and
PEGylating biomolecules to reduce immunogenicity and increase water solubility.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 2339.63; single compound
Chemical formula C₁₀₄H₁₉₁F₄N₅O₄₇
CAS N/A
Purity > 96%
Spacers dPEG® Spacer is 63 atoms and 74.1 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post TFP-dPEG®₄-(m-dPEG®₁₁)₃-ester appeared first on VectorLabs.

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Description

TFP-dPEG®4-(m-dPEG®11)3 ester, product number QBD-11401, is a water-soluble, amine-reactive, discrete PEG (dPEG®) PEGylation reagent built. This product is built around a tris core and consists of a short (21 atoms) amine-reactive 2,3,5,6-tetrafluorophenyl (TFP) ester “”tail”” on a uniform (i.e., monodispersed) PEG4 tail and three (3) medium-length (35 atoms) branches composed from methyl-terminated dPEG®11 (m-dPEG®11). The total dPEG® spacer length from the reactive end to the methyl termini is 63 atoms (74.1 Å average). TFP-dPEG®4-(m-dPEG®11)3 ester is used to PEGylate and modify amine-reactive biomolecules and surfaces. This hydrophilic branched dPEG® product greatly increases the conjugates’ hydrodynamic volumes, reducing immunogenicity and renal clearance. Moreover, this product reduces or eliminates non-specific binding to conjugates and surfaces.

TFP esters react with free amines like NHS esters to form stable amide bonds. TFP esters’ optimal reaction pH is 7.5 – 8.0, which is higher than NHS esters (optimal pH range 7.0 – 7.5). However, published research has shown that, within the optimal reaction pH range, TFP esters are more reactive toward amines than NHS esters are within their optimal pH range. Applications for TFP-dPEG®4-(m-dPEG®11)3 ester, product number QBD-11401, include the following:
conjugating proteins and other biomolecules to modify PK and BD;
PEGylating amine-coated surfaces (e.g., nanoparticles) to sterically shield the surfaces and reduce non-specific, hydrophobic interactions;
PEGylating amine-coated organic or inorganic surfaces (e.g., carbon, gold, silver nanoparticles) to reduce or eliminate immunogenicity;
PEGylating biomolecules to reduce renal clearance and increase serum half-life; and
PEGylating biomolecules to reduce immunogenicity and increase water solubility.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight2339.63; single compound
Chemical formulaC₁₀₄H₁₉₁F₄N₅O₄₇
CASN/A
Purity> 96%
SpacersdPEG® Spacer is 63 atoms and 74.1 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Synthesis and Preliminary Biological Evaluation of High-Drug-Load Paclitaxel-Antibody Conjugates for Tumor-Targeted Chemotherapy, Sherly Quiles, Kevin P. Raisch, Leisa L. Sanford, James A. Bonner, and Ahmad Safavy, J Med. Chem., 2010, 53 (2), pp 586-594, December 3, 2009. DOI: 10.1021/jm900899g.

Visualizing Systemic Clearance and Cellular Level Biodistribution of Gold Nanorods by Intrinsic Two-Photon Luminescence, Ling Tong, Wei He, Yanshu Zhang, Wei Zheng, and Ji-Xin Cheng, Langmuir 2009, 25 (21), pp 12454-12459. DOI: 10.1021/la902992w.

A New Polyethyleneglycol-Derivatized Hemoglobin Derivative with Decreased Oxygen Affinity and Limited Toxicity. Oana Zolog, Augustin Mot, Florina Deac, Alina Roman, Eva Fischer-Fodor, Radu Silaghi-Dumitrescu. The Protein Journal. 2010 1 (30), pp 27-31. December 16, 2012 DOI: 10.1007/s10930-010-9298-5.

Improved pharmacokinetics and immunogenicity profile of organophosphorus hydrolase by chemical modification with polyethylene glycol. Boris N. Novikov, Janet K. Grimsley, Rory J. Kern, James R. Wild, Melinda E. Wales. Elsevier. 2010 146 (3) Pages 318-325 September 15, 2010. DOI: 10.1016/j.jconrel.2010.06.003.

The post TFP-dPEG®₄-(m-dPEG®₁₁)₃-ester appeared first on VectorLabs.

]]> NHS-dPEG®₄-(m-dPEG®₈)₃-ester https://staging.vectorlabs.com/products/nhs-dpeg4-m-dpeg83-ester/ Sat, 24 Feb 2024 02:10:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=36179 NHS-dPEG®4-(m-dPEG®8)3 ester, product number QBD-10421, is a water-soluble, amine-reactive, single molecular weight, branched PEGylation reagent built around a tris core. A short dPEG®4 arm ends with an N-hydroxysuccinimide (NHS) ester. Three equal-length dPEG®8 branches terminate with methyl groups. The distance from the terminal carbonyl carbon of the dPEG®4 arm to the terminal methyl group on each of the dPEG®8 branches is 55 atoms (60.2 Å). This hydrophilic, branched dPEG® product increases the conjugates’ water solubility and hydrodynamic volume. Conjugates also may display reduced immunogenicity, diminished renal clearance, and lowered non-specific hydrophobic interactions.

The reaction chemistry between the NHS ester and a free amine is standard in almost all bioconjugation labs. NHS-dPEG®4-(m-dPEG®8)3 ester can be used for:
Coating nanoparticle surfaces;
Creating imaging applications;
Developing Antibody-Drug Conjugates (ADCs);
Modifying PK and BD of biomolecular conjugates;
Preventing non-specific hydrophobic interactions; and,
Passivating amine-functionalized inorganic surfaces such as metal and diamond.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 1892.17; single compound
Chemical formula C₈₄H₁₅₈N₆O₄₀
CAS 1333154-74-7
Purity > 96%
Spacers dPEG® Spacer is 55 atoms and 60.2 Å, Avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post NHS-dPEG®₄-(m-dPEG®₈)₃-ester appeared first on VectorLabs.

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Description

NHS-dPEG®4-(m-dPEG®8)3 ester, product number QBD-10421, is a water-soluble, amine-reactive, single molecular weight, branched PEGylation reagent built around a tris core. A short dPEG®4 arm ends with an N-hydroxysuccinimide (NHS) ester. Three equal-length dPEG®8 branches terminate with methyl groups. The distance from the terminal carbonyl carbon of the dPEG®4 arm to the terminal methyl group on each of the dPEG®8 branches is 55 atoms (60.2 Å). This hydrophilic, branched dPEG® product increases the conjugates’ water solubility and hydrodynamic volume. Conjugates also may display reduced immunogenicity, diminished renal clearance, and lowered non-specific hydrophobic interactions.

The reaction chemistry between the NHS ester and a free amine is standard in almost all bioconjugation labs. NHS-dPEG®4-(m-dPEG®8)3 ester can be used for:
Coating nanoparticle surfaces;
Creating imaging applications;
Developing Antibody-Drug Conjugates (ADCs);
Modifying PK and BD of biomolecular conjugates;
Preventing non-specific hydrophobic interactions; and,
Passivating amine-functionalized inorganic surfaces such as metal and diamond.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight1892.17; single compound
Chemical formulaC₈₄H₁₅₈N₆O₄₀
CAS1333154-74-7
Purity> 96%
SpacersdPEG® Spacer is 55 atoms and 60.2 Å, Avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetonitrile, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post NHS-dPEG®₄-(m-dPEG®₈)₃-ester appeared first on VectorLabs.

]]> NHS-dPEG®₄-(m-dPEG®₁₂)₃-ester https://staging.vectorlabs.com/products/nhs-dpeg4-m-dpeg123-ester/ Sat, 24 Feb 2024 02:10:23 +0000 https://staging.vectorlabs.com/?post_type=product&p=36155 NHS-dPEG®4-(m-dPEG®12)3 ester, product number QBD-10401, is a water-soluble, amine-reactive, single molecular weight, branched PEGylation reagent built around a tris core and used to modify amine-functionalized surfaces and biomolecules. Three equal-length dPEG®12 branches terminate with methyl groups. A short dPEG®4 arm ends with an amine-reactive N-hydroxysuccinimidyl (NHS) ester. From the terminal carbonyl carbon of the dPEG®4 arm to each terminal methyl group on the dPEG®12 branches, the distance is 66 atoms (51.1 Å) long. This hydrophilic branched dPEG® product adds hydrodynamic volume to the conjugates, which reduces immunogenicity and renal clearance. Moreover, this product reduces or eliminates non-specific binding to conjugates and surfaces.

The reaction chemistry for coupling a free amine to an NHS ester is standard in almost all bioconjugation labs worldwide. Numerous scientific publications attest to the benefits of NHS-dPEG®4-(m-dPEG®12)3 ester. These reports describe this product being used to:
Coat nanoparticle surfaces;
Create imaging applications;
Develop Antibody-Drug Conjugates (ADCs);
Modify PK and BD of biomolecular conjugates;
Prevent non-specific binding;
Passivate inorganic surfaces such as metal and diamond; and,
Develop hemerythrin-based blood substitutes.

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 2420.80; single compound
Chemical formula C₁₀₈H₂₀₆N₆O₅₂
CAS 1334178-03-8
Purity > 96%
Spacers dPEG® Spacer is 66 atoms and 51.1 Å, avg.
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or Acetonitrile.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post NHS-dPEG®₄-(m-dPEG®₁₂)₃-ester appeared first on VectorLabs.

]]>

Description

NHS-dPEG®4-(m-dPEG®12)3 ester, product number QBD-10401, is a water-soluble, amine-reactive, single molecular weight, branched PEGylation reagent built around a tris core and used to modify amine-functionalized surfaces and biomolecules. Three equal-length dPEG®12 branches terminate with methyl groups. A short dPEG®4 arm ends with an amine-reactive N-hydroxysuccinimidyl (NHS) ester. From the terminal carbonyl carbon of the dPEG®4 arm to each terminal methyl group on the dPEG®12 branches, the distance is 66 atoms (51.1 Å) long. This hydrophilic branched dPEG® product adds hydrodynamic volume to the conjugates, which reduces immunogenicity and renal clearance. Moreover, this product reduces or eliminates non-specific binding to conjugates and surfaces.

The reaction chemistry for coupling a free amine to an NHS ester is standard in almost all bioconjugation labs worldwide. Numerous scientific publications attest to the benefits of NHS-dPEG®4-(m-dPEG®12)3 ester. These reports describe this product being used to:
Coat nanoparticle surfaces;
Create imaging applications;
Develop Antibody-Drug Conjugates (ADCs);
Modify PK and BD of biomolecular conjugates;
Prevent non-specific binding;
Passivate inorganic surfaces such as metal and diamond; and,
Develop hemerythrin-based blood substitutes.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight2420.80; single compound
Chemical formulaC₁₀₈H₂₀₆N₆O₅₂
CAS1334178-03-8
Purity> 96%
SpacersdPEG® Spacer is 66 atoms and 51.1 Å, avg.
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or Acetonitrile.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Towards the Development of Hemerythrin-Based Blood Substitutes. Augustin C. Mot, Alina Roman, Iulia Lupan , Donald M. Kurtz Jr , Radu Silaghi-Dumitrescu. Protein J. 2010, 29 (6) pp 87-393. June 27, 2010. DOI: 10.1007/s10930-010-9264-2.

Rapid Raman Imaging of Stable, Functionalized Nanoshells in Mammalian Cell Cultures. Yiming Huang, Vimal P. Swarup, Sandra Whaley Bishnoi. Nano Letters. 2009, 9 (8) pp 2914-2920. May 23, 2009. DOI: 10.1021/nl901234x.

Differential patterning of neuronal, glial and neural progenitor cells on phosphorus-doped and UV irradiated diamond-like carbon. Edward M. Regan, James B. Uney, Andrew D. Dick, Yiwei Zhang, Jose Nunez-Yanez, Joseph P. McGeehan, Frederik Claeyssens, Stephen Kelly. Biomaterials. 2010, 31 (2) pp 207-215. October 14, 2009. DOI: 10.1016/j.biomaterials.2009.09.042.

Different radiolabelling methods alter the pharmacokinetic and biodistribution properties of Plasminogen Activator Inhibitor Type 2 (PAI-2) forms. Marie Ranson, Paula Berghofer, Kara L. Vine, Ivan Greguric, Rachael Shepherd, Andrew Katsifis. Nuclear Medicine and Biology. 2012, 39 (6) pp 833-839. January 17, 2012. DOI: 10.1016/j.nucmedbio.2012.01.006.

Circulation and long-term fate of functionalized, biocompatible single-walled carbon nanotubes in mice probed by Raman spectroscopy. Zhuang Liu, Corrine Davis, Weibo Cai, Lina He, Xiaoyuan Chen, and Hongjie Dai. PNAS. 2008, 105 (5) pp 1410-1415. February 5, 2008. DOI: 10.1073_pnas.0707654105.

Synthesis and Preliminary Biological Evaluation of High-Drug-Load Paclitaxel-Antibody Conjugates for Tumor-Targeted Chemotherapy. Sherly Quiles, Kevin P. Raisch, Leisa L. Sanford, James A. Bonner, and Ahmad Safavy. Journal of Medicinal Chemistry. 2009, 53 (2) pp 586-594. December 3, 2009. DOI: 10.1021/jm900899g.

TIRF microscopy as a screening method for non-specific binding on surfaces. Christy Charlton, Vladimir Gubala, Ram Prasad Gandhiraman, Julie Wiechecki, Nam Cao Hoai Le, Conor Coyle, Stephen Daniels, Brian D. MacCraith, David E. Williams. Journal of Colloid and Interface Science. 2011, 354 (1) pp 405-409. February 1, 2011. DOI: 10.1016/j.jcis.2010.10.029.

Influence of PEGylation with linear and branched PEG chains on the adsorption of glucagon to hydrophobic surfaces. Charlotte Pinholt , Jens Thostrup Bukrinsky, Susanne Hostrup, Sven Frokjaer, Willem Norde, Lene Jorgensen. European Journal of Pharmaceutics and Biopharmaceutics. 2010, 77 (1) pp 139–147. November 3, 2010. DOI: 10.1016/j.ejpb.2010.11.001.

The influence of polyethylence glycol structure on the conjugation of recombinant human interferon a2b overproduced using synthetic gene in E. coli. Heni Rachmawati, Prima L. Febrina, Ratih A, Ningrum, Debbie S. Retnoningrum. International Journal of Research in Pharmaceutical Sciences. 2012, 3(2) pp 228-233. March 20, 2012. ISSN: 0975-7538.

Monitoring Protein PEGylation with Ion Exchange Chromatography. Peter Yu, Deanna Hurum, Leo Wang, Terry Zhang,and Jeffrey Rohrer. ThermoFisher Scientific Poster Note. 2013, PN70510_E01/3s.

New Assay for Quantification of PEGylated Proteins During in Vitro Permeability Studies. Spela Jalen, Vanja Smilovic, Katarina Fidler, Barbara Podobnik, Maja Marusic, Radovan Komel, Vladka Gaberc-Porekar and Simona Jevsevar. Acta Chimica Slovenica. 2014, 61 (3) pp 615-622. January 15, 2014.

Nanometer size silicon particles for hyperpolarized MRI. Grzegorz Kwiatkowski, Fabian Jahnig, Jonas Steinhauser, Patrick Wespi, Matthias Ernst, and Sebastian Kozerke. Scientific Reports. 2017, 7 (7946) pp 1-9. August 11, 2017. DOI: 10.1038/s41598-017-08709-0.

Aptamer-Modified Nanohydrogel Microarrays for Bioselective Cancer Cell Immobilization. Zan Lamberger, Hendrik Bargel, Martin Humenik. Advanced Functional Materials, Volume 32, Issue 45 (2022). 08/28/22. https://doi.org/10.1002/adfm.202207270

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