Biotinylated – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:41 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotinylated – VectorLabs https://staging.vectorlabs.com 32 32 Aleuria Aurantia Lectin (AAL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-aleuria-aurantia-lectin-aal/ Thu, 20 Apr 2023 19:19:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=11108 Description

Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids.

Biotinylated Aleuria Aurantia Lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Storage 2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar 100-400 mM L-fucose
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Fucose, Arabinose

Technical Information

This lectin is a dimer of two identical subunits of about 36 kDa each.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 100 mM L-fucose

The post Aleuria Aurantia Lectin (AAL), Biotinylated appeared first on VectorLabs.

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Description

Unlike Ulex europaeus and Lotus tetragonolobus lectins which prefer (α -1,2) linked fucose residues, Aleuria aurantia lectin binds preferentially to fucose linked (α -1,6) to N-acetylglucosamine or to fucose linked (α -1,3) to N-acetyllactosamine related structures. AAL also reversibly binds fucose attached to nucleic acids.

Biotinylated Aleuria Aurantia Lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Storage2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar100-400 mM L-fucose
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityFucose, Arabinose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This lectin is a dimer of two identical subunits of about 36 kDa each. 

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 100 mM L-fucose

The post Aleuria Aurantia Lectin (AAL), Biotinylated appeared first on VectorLabs.

]]> Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated https://staging.vectorlabs.com/products/biotinylated-narcissus-pseudonarcissus/ Thu, 20 Apr 2023 19:14:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=11101 Description

NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated

Technical Information

This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 400 mM α-methylmannoside

The post Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated appeared first on VectorLabs.

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Description

NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.

Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityMannose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Technical Information

This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers. 

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting Sugar: 400 mM α-methylmannoside

The post Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated appeared first on VectorLabs.

]]> Wisteria Floribunda Lectin (WFA, WFL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-wisteria-floribunda-lectin/ Thu, 20 Apr 2023 19:05:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=11094 Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Biotinylated WFL has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity N-Acetylgalactosamine

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

The post Wisteria Floribunda Lectin (WFA, WFL), Biotinylated appeared first on VectorLabs.

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Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Biotinylated WFL has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityN-Acetylgalactosamine

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

The post Wisteria Floribunda Lectin (WFA, WFL), Biotinylated appeared first on VectorLabs.

]]> Lotus Tetragonolobus Lectin (LTL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-lotus-tetragonolobus-lectin-ltl/ Thu, 20 Apr 2023 17:03:04 +0000 https://staging.vectorlabs.com/?post_type=product&p=11087 Description

Lotus tetragonolobus lectin is a family of closely related glycoproteins that appear to have similar specificities toward α-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are significantly different between these fucose-specific lectins.

Biotinylated Lotus tetragonolobus has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute by adding 1ml water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Conjugate Biotinylated
Sugar Specificity Fucose, Arabinose

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

The post Lotus Tetragonolobus Lectin (LTL), Biotinylated appeared first on VectorLabs.

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Description

Lotus tetragonolobus lectin is a family of closely related glycoproteins that appear to have similar specificities toward α-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are significantly different between these fucose-specific lectins.

Biotinylated Lotus tetragonolobus has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageReconstitute by adding 1ml water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
ConjugateBiotinylated
Sugar SpecificityFucose, Arabinose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

The post Lotus Tetragonolobus Lectin (LTL), Biotinylated appeared first on VectorLabs.

]]> Maackia Amurensis Lectin I (MAL I), Biotinylated https://staging.vectorlabs.com/products/biotinylated-maackia-amurensis-lectin-i/ Thu, 20 Apr 2023 16:41:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=11080 Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute in 1 ml water. The resulting solution will be: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% NaN3, 0.1 mM CaCl2, 0.01 mM MnCl2.
Recommended Storage 2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar 200 mM lactose (S-9004).
Conjugate Biotinylated
Sugar Specificity Galactose, Lactose

Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose

The post Maackia Amurensis Lectin I (MAL I), Biotinylated appeared first on VectorLabs.

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Description

Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.

Biotinylated Maackia amurensis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageReconstitute in 1 ml water. The resulting solution will be: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% NaN3, 0.1 mM CaCl2, 0.01 mM MnCl2.
Recommended Storage2-8 °C; Store frozen for long term storage
Inhibiting and/or Eluting Sugar200 mM lactose (S-9004).
ConjugateBiotinylated
Sugar SpecificityGalactose, Lactose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This lectin is the leucoagglutinin or mitogenic isolectin from Maackia seeds.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibiting Sugar: 200 mM lactose

The post Maackia Amurensis Lectin I (MAL I), Biotinylated appeared first on VectorLabs.

]]> Sambucus Nigra Lectin (SNA, EBL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-sambucus-nigra-lectin-sna-ebl/ Thu, 20 Apr 2023 16:37:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=11070 Description

Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.

Biotinylated Sambucus nigra lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Please note that SNL and SNA are equivalent. Historically, Vector Laboratories has used SNL as the acronym for Sambucus Nigra Lectin. SNA, reflective of Sambucus Nigra Agglutinin, is commonly used  within the scientific community for this product, e.g., as shown for B-1305 on the NCFG website. Please contact us at customerservice@staging.vectorlabs.com or at +1-800-227-6666 / +1-650-697-3600 should you have any questions regarding this matter.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 10 µg/ml in the below buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Sialic Acid

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid

The post Sambucus Nigra Lectin (SNA, EBL), Biotinylated appeared first on VectorLabs.

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Description

Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.

Biotinylated Sambucus nigra lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Please note that SNL and SNA are equivalent. Historically, Vector Laboratories has used SNL as the acronym for Sambucus Nigra Lectin. SNA, reflective of Sambucus Nigra Agglutinin, is commonly used  within the scientific community for this product, e.g., as shown for B-1305 on the NCFG website. Please contact us at customerservice@staging.vectorlabs.com or at +1-800-227-6666 / +1-650-697-3600 should you have any questions regarding this matter.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 10 µg/ml in the below buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, 015 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificitySialic Acid

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid

The post Sambucus Nigra Lectin (SNA, EBL), Biotinylated appeared first on VectorLabs.

]]> Maackia Amurensis Lectin II (MAL II), Biotinylated https://staging.vectorlabs.com/products/biotinylated-maackia-amurensis-lectin-ii/ Thu, 20 Apr 2023 16:33:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=11063 Description

Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.

Biotinylated Maackia amurensis lectin II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 1 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide.
Concentration 1 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity Sialic Acid

Technical Information

Maackia amurensis lectin II (MAH) is a glycoprotein consisting of two subunits each of which is composed of disulfide-linked chains.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibitor: Human glycophorin

The post Maackia Amurensis Lectin II (MAL II), Biotinylated appeared first on VectorLabs.

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Description

Although the specificity of this lectin is not well defined, MAL II appears to bind only particular carbohydrate structures that contain sialic acid. Unlike Sambucus nigra lectin (SNA) which seems to prefer structures with (α-2,6) linked sialic acid, MAL II appears to bind sialic acid in an (α-2,3) linkage. Tissue staining patterns are also very different among MAL I, SNA and MAL II.

Biotinylated Maackia amurensis lectin II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide.
Concentration1 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificitySialic Acid

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

Maackia amurensis lectin II (MAH) is a glycoprotein consisting of two subunits each of which is composed of disulfide-linked chains.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Geisler, C and Jarvis, D.L. Glycobiology, vol. 21, no. 8 pp. 988-993, 2011

Inhibitor: Human glycophorin

The post Maackia Amurensis Lectin II (MAL II), Biotinylated appeared first on VectorLabs.

]]> Galanthus Nivalis Lectin (GNL), Biotinylated https://staging.vectorlabs.com/products/biotinylated-galanthus-nivalis-lectin-gnl/ Wed, 19 Apr 2023 21:49:09 +0000 https://staging.vectorlabs.com/?post_type=product&p=11053 Description

Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding.

Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.

Biotinylated Galanthus nivalis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration 2 mg/ml
Conjugate Biotinylated
Sugar Specificity Mannose

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside

The post Galanthus Nivalis Lectin (GNL), Biotinylated appeared first on VectorLabs.

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Description

Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. 

Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins. 

Biotinylated Galanthus nivalis lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer. A precipitate may form during storage.This does not have a significant adverse effect on the product.If a precipitate forms upon long-term storage, warm to 37 ºC and centrifuge before use.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.
Concentration2 mg/ml
ConjugateBiotinylated
Sugar SpecificityMannose

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 100 mM – 200 mM α-methylmannoside

The post Galanthus Nivalis Lectin (GNL), Biotinylated appeared first on VectorLabs.

]]> Vicia Villosa Lectin (VVL, VVA), Biotinylated https://staging.vectorlabs.com/products/biotinylated-vicia-villosa-lectin-vvl-vva/ Wed, 19 Apr 2023 21:44:55 +0000 https://staging.vectorlabs.com/?post_type=product&p=11046 Description

VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.

Biotinylated Vicia villosa lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration 2 mg active conjugate/ml
Conjugate Biotinylated
Sugar Specificity N-Acetylgalactosamine

Technical Information

This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich.

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

The post Vicia Villosa Lectin (VVL, VVA), Biotinylated appeared first on VectorLabs.

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Description

VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). Evidence suggests that this lectin also may require specific amino acid sequences at the receptor site of glycosylation. The disaccharide galactosyl (α-1,3) N-acetylgalactosamine is also a potent inhibitor of this lectin.

Biotinylated Vicia villosa lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

More Information
Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageFor most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
Solution10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
Concentration2 mg active conjugate/ml
ConjugateBiotinylated
Sugar SpecificityN-Acetylgalactosamine

Documents

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. 

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

The post Vicia Villosa Lectin (VVL, VVA), Biotinylated appeared first on VectorLabs.

]]> Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated https://staging.vectorlabs.com/products/biotinylated-griffonia-simplicifolia-gsl/ Wed, 19 Apr 2023 21:39:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=11039 Description

Griffonia (Bandeiraea) Simplicifolia Lectin II is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.

Biotinylated GSL II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size 2 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute biotinylated lectin by addition 1 ml of water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-Acetylglucosamine.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage 2-8 °C; Store frozen for long term storage
Conjugate Biotinylated
Sugar Specificity N-Acetylglucosamine

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine

The post Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated appeared first on VectorLabs.

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Description

Griffonia (Bandeiraea) Simplicifolia Lectin II is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linkedN-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides. 

Biotinylated GSL II has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

Specifications

Unit Size2 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageReconstitute biotinylated lectin by addition 1 ml of water.The resulting solution will have the following composition:10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-Acetylglucosamine.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.
Recommended Storage2-8 °C; Store frozen for long term storage
ConjugateBiotinylated
Sugar SpecificityN-Acetylglucosamine

Documents

 

Product FAQs

From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.

Citations

Technical Information

This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine

The post Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Biotinylated appeared first on VectorLabs.

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