Inhibiting Sugars and Eluting Solutions – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:11:17 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Inhibiting Sugars and Eluting Solutions – VectorLabs https://staging.vectorlabs.com 32 32 Chitin Hydrolysate https://staging.vectorlabs.com/products/chitin-hydrolysate/ Mon, 01 May 2023 23:57:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=13744 Description

Chitin Hydrolysate is supplied as a highly concentrated solution of N-acetylglucosamine (glcNAc) and glcNAc oligomers in nearly saturated sodium chloride.

Chitin Hydrolysate (SP-0090) can be used as an inhibitor of lectin-conjugate binding or for eluting glycoproteins bound to agarose lectins. This mixture is an inhibitor for the following lectins: DSL, GSL II, LEL, STL, and WGA.

Specifications

Unit Size 10 ml
Applications Glycobiology, Affinity Chromatography
Solution Nearly saturated sodium chloride in water
Recommended Usage If a precipitate forms during storage do not warm solution.This may cause further precipitation.Centrifuge or filter the hydrolysate to remove precipitated material.
Recommended Storage 2-8 °C

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Description

Chitin Hydrolysate is supplied as a highly concentrated solution of N-acetylglucosamine (glcNAc) and glcNAc oligomers in nearly saturated sodium chloride.

Chitin Hydrolysate (SP-0090) can be used as an inhibitor of lectin-conjugate binding or for eluting glycoproteins bound to agarose lectins. This mixture is an inhibitor for the following lectins: DSL, GSL II, LEL, STL, and WGA.

Specifications

Unit Size10 ml
ApplicationsGlycobiology, Affinity Chromatography
SolutionNearly saturated sodium chloride in water
Recommended UsageIf a precipitate forms during storage do not warm solution.This may cause further precipitation.Centrifuge or filter the hydrolysate to remove precipitated material.
Recommended Storage2-8 °C

Documents

Citations

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]]> N-acetylneuraminic acid (sialic acid) https://staging.vectorlabs.com/products/n-acetylneuraminic-acid-sialic-acid/ Fri, 28 Apr 2023 20:52:17 +0000 https://staging.vectorlabs.com/?post_type=product&p=12994 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size 619 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical Formula C11H19NO9
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight 309.3

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size619 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical FormulaC11H19NO9
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight309.3

Documents

Citations

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]]> L-fucose https://staging.vectorlabs.com/products/l-fucose/ Fri, 28 Apr 2023 20:50:12 +0000 https://staging.vectorlabs.com/?post_type=product&p=12987 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 100 mM.

Specifications

Unit Size 82 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 100 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical Formula C6H12O5
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight 164.2

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 100 mM.

Specifications

Unit Size82 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 100 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical FormulaC6H12O5
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight164.2

Documents

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]]> α-methylglucoside https://staging.vectorlabs.com/products/a-methylglucoside/ Fri, 28 Apr 2023 20:48:13 +0000 https://staging.vectorlabs.com/?post_type=product&p=12980 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size 388 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical Formula C7H14O6
Recommended Storage Reconstited sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight 194.2

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size388 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM. This sugar is suitable for diluting labeled lectins to demonstrate lectin specificity by inhibiting binding or for eluting glycoconjugates from agarose-bound lectins.
Chemical FormulaC7H14O6
Recommended StorageReconstited sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.
Molecular Weight194.2

Documents

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]]> α-methylmannoside https://staging.vectorlabs.com/products/a-methylmannoside/ Fri, 28 Apr 2023 20:46:11 +0000 https://staging.vectorlabs.com/?post_type=product&p=12973 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water, the resulting sugar solution is 400 mM.

Specifications

Unit Size 388 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water, the resulting sugar solution is 400 mM.

Specifications

Unit Size388 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

Documents

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]]> Lactose https://staging.vectorlabs.com/products/lactose/ Fri, 28 Apr 2023 20:43:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=12966 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

 
Unit Size 721 mg
Applications Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

More Information
Unit Size721 mg
ApplicationsGlycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

Documents

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]]> Galactose https://staging.vectorlabs.com/products/galactose/ Fri, 28 Apr 2023 20:41:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=12959 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder and can be reconstituted in 5 ml of distilled or deionized water or in 5 ml of a buffer or salt solution compatible with the lectin under study. If reconstituted in 5 ml the resulting sugar concentration is 400 mM. The sugar solution can be diluted further when appropriate or the sugar may be reconstituted with a smaller volume of water or buffer, in the sugar is soluble, to increase the final concentration

Specifications

More Information
Unit Size 360 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder and can be reconstituted in 5 ml of distilled or deionized water or in 5 ml of a buffer or salt solution compatible with the lectin under study. If reconstituted in 5 ml the resulting sugar concentration is 400 mM. The sugar solution can be diluted further when appropriate or the sugar may be reconstituted with a smaller volume of water or buffer, in the sugar is soluble, to increase the final concentration.

Specifications

More Information
Unit Size360 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

Documents

Citations

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]]> N-acetylglucosamine https://staging.vectorlabs.com/products/n-acetylglucosamine/ Fri, 28 Apr 2023 20:38:30 +0000 https://staging.vectorlabs.com/?post_type=product&p=12952 Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size 442 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

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Description

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 400 mM.

Specifications

Unit Size442 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 400 mM.
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

Documents

Citations

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]]> N-acetylgalactosamine https://staging.vectorlabs.com/products/n-acetylgalactosamine/ Fri, 28 Apr 2023 20:35:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=12945 Description

Features:

  • Provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glucoconjugates from columns of agarose-lectins
  • Supplied as dry powder
  • If reconstituted in 5 ml of water or buffer, the resulting sugar solution is 100 mM

Specifications

Unit Size 111 mg
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended Usage Reconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 100 mM.
Recommended Storage Reconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

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Description

Features:

  • Provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glucoconjugates from columns of agarose-lectins
  • Supplied as dry powder
  • If reconstituted in 5 ml of water or buffer, the resulting sugar solution is 100 mM

Specifications

Unit Size111 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Glycobiology, Affinity Chromatography
Recommended UsageReconstitute the sugar in 5 ml of distilled or deionized water or in 5 ml of a buffer (e.g. 10 mM HEPES buffered saline, pH 7.5-pH 8.0) or salt solution compatible with the lectin under study. If reconstituted in 5 ml, this sugar will be 100 mM.
Recommended StorageReconstituted sugar solutions should be stored frozen or stored at 4 ºC using sodium azide (0.08%) as a preservative.

Documents

Citations

Technical Information

This sugar is provided for use as an inhibitor of lectin-conjugate binding or for eluting glycoproteins or other glycoconjugates from columns of agarose lectins. This sugar is supplied as a dry powder. When reconstituted in 5 ml of water the resulting sugar solution is 100 mM.

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]]> Glycoprotein Eluting Solution for Sialic Acid Binding Lectins https://staging.vectorlabs.com/products/glycoprotein-eluting-solution-for-sialic-acid/ Tue, 25 Apr 2023 15:57:24 +0000 https://staging.vectorlabs.com/?post_type=product&p=11809 Description

Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.

Features:

  • All components can be subsequently removed by dialysis

Specifications

Unit Size 100 ml
Applications Glycobiology, Affinity Chromatography
Recommended Usage 1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration.
Recommended Storage Room Temperature

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Description

Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.

Features:

  • All components can be subsequently removed by dialysis

Specifications

Unit Size100 ml
ApplicationsGlycobiology, Affinity Chromatography
Recommended Usage1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration.
Recommended StorageRoom Temperature

Documents

Product FAQs

The glycoprotein eluting solutions are largely comprised of specific sugars and defined salts that are usually required to remove bound material from agarose conjugated lectins. These eluting solutions do not introduce harsh pH conditions or other factors that may affect the eluted glycoproteins or the lectin column. As such, we view these mixtures as being both eluting and regeneration solutions. None of these solutions contains protein.

Technical Information

Glycoproteins are frequently isolated and purified from protein mixtures using columns of agarose-bound lectins. After applying a protein mixture, the agarose-lectin column is washed free of unwanted proteins and the glycoprotein bound to the lectin is eluted with a sugar that inhibits binding. Unfortunately, achieving complete elution with a simple sugar solution can be difficult. 

NeoGlyco3 1200 jpg

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