Glycan Screening Kits – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:19 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Glycan Screening Kits – VectorLabs https://staging.vectorlabs.com 32 32 Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling https://staging.vectorlabs.com/products/click-go-isotag-kit-for-intact-glycopeptides-profiling-2/ Tue, 19 Sep 2023 19:51:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=22185 Specifications
Enrichment target Azide-modified proteins
Enrichment Technology Biotin-streptavidin based enrichment
Number of Enrichments 25
Storage Conditions 4C
Shipping Conditions Ambient temperature

Description

While there has been much interest in profiling the intact glycoproteome, the complexity of glycoproteoforms (and more broadly, all proteoforms) remains challenging to completely define. Mass spectrometry (MS) is commonly employed for characterization of complex proteomic samples. A popular strategy for protein identification is the bottom-up shotgun proteomics approach. In this method, a mixture of proteins is subjected to proteolytic digestion, the resulting peptides are separated by LC and detected by MS, and their parent proteins are inferred from the assigned peptide sequences.

To convert MS data acquired from proteolytic digests into protein identifications, tandem MS can be used to obtain sequence information for individual peptides, followed by comparing an in-silico proteolytic digest of an organism’s proteome. Typically, only the most abundant peptides are selected for fragmentation (Figure 2), whereas data for those peptides in relatively low quantities are not obtained. An inherent problem in shotgun proteomics is identifying proteins of low abundance, such as biomarkers for disease states, against a background of proteins whose concentrations can span up to 12 orders of magnitude.

Figure 1. Metabolic labeling with a chemically functionalized glycan, chemical tagging and enrichment using an isotopic recoding affinity probe

To address the unique challenges of the global characterization of the intact glycoproteome, a mass-independent chemical glycoproteomics platform, termed isotope targeted glycoproteomics (IsoTag) was developed by the Carolyn Bertozzi group. The platform is comprised of four central components: (i) metabolic labeling with a chemically functionalized glycan, (ii) chemical tagging and enrichment using an isotopic recoding affinity probe, (iii) directed tandem MS, and (iv) targeted glycopeptide assignment (Figure 2).

Figure 2. Traditional proteomics and Iso-Tag-directed proteomics workflow

IsoTaG is performed by isotopic recoding and enrichment of metabolically labeled glycoproteins followed by directed tandem MS (MS2 or MSn) analysis and intact glycopeptide assignment. Isotopic recoding is accomplished by metabolic labeling of cell or tissue samples with azide- or alkyne-functionalized sugars, followed by chemical conjugation with a biotin probe bearing a unique isotopic signature.

Some examples of sugar labels are peracetylated N-azidoacetylmannosamine (Ac4ManNAz), which is converted to the corresponding azidosialic acid (SiaNAz), and peracetylated N-azidoacetylgalactosamine (Ac4GalNAz), which is metabolized to label glycans possessing N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) (not provided with kit).

In order to perform isotopic tagging, the kit provides two cleavable IsoTaG probes encoded by zero [M] and two [M + 2] deuterium atoms. Probes with different encoding can be provided by Click Chemistry Tools though custom synthesis. The IsoTaG probe with zero, and that with two deuterium atoms [M, M + 2] can be used in different proportions; 1:1, 1:2, 1:3 and 1:4. Pattern recognition with isotopic ratio of 1:3 showed the highest fidelity.

Figure 3. Cleavable IsoTaG probe encoded by zero deuterium atoms [M] (R = H) and two deuterium atoms [M+2] (R = D)

Through these probes, a unique isotopic signature is embedded exclusively into the glycopeptides. The isotopic signature serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recorded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group. IsoStamp compares observed and predicted isotopic envelopes to identify chemically tagged species in full-scan mass spectra.

IsoTag has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope-coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for post-translational modification.

[caption id="attachment_26280" align="alignnone" width="380"] Schematic Workflow[/caption]

The post Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling appeared first on VectorLabs.

]]>
IsoTaG is a mass-independent chemical glycoproteomics platform that helps to address unique challenges of the global characterization of the intact glycoproteome. In this approach a unique isotopic signature is embedded exclusively into the glycopeptides and it serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recoded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group.

Description

While there has been much interest in profiling the intact glycoproteome, the complexity of glycoproteoforms (and more broadly, all proteoforms) remains challenging to completely define. Mass spectrometry (MS) is commonly employed for characterization of complex proteomic samples. A popular strategy for protein identification is the bottom-up shotgun proteomics approach. In this method, a mixture of proteins is subjected to proteolytic digestion, the resulting peptides are separated by LC and detected by MS, and their parent proteins are inferred from the assigned peptide sequences.

To convert MS data acquired from proteolytic digests into protein identifications, tandem MS can be used to obtain sequence information for individual peptides, followed by comparing an in-silico proteolytic digest of an organism’s proteome. Typically, only the most abundant peptides are selected for fragmentation (Figure 2), whereas data for those peptides in relatively low quantities are not obtained. An inherent problem in shotgun proteomics is identifying proteins of low abundance, such as biomarkers for disease states, against a background of proteins whose concentrations can span up to 12 orders of magnitude.

iso tag img one
Figure 1. Metabolic labeling with a chemically functionalized glycan, chemical tagging and enrichment using an isotopic recoding affinity probe

To address the unique challenges of the global characterization of the intact glycoproteome, a mass-independent chemical glycoproteomics platform, termed isotope targeted glycoproteomics (IsoTag) was developed by the Carolyn Bertozzi group. The platform is comprised of four central components: (i) metabolic labeling with a chemically functionalized glycan, (ii) chemical tagging and enrichment using an isotopic recoding affinity probe, (iii) directed tandem MS, and (iv) targeted glycopeptide assignment (Figure 2).

iso tag img two
Figure 2. Traditional proteomics and Iso-Tag-directed proteomics workflow

IsoTaG is performed by isotopic recoding and enrichment of metabolically labeled glycoproteins followed by directed tandem MS (MS2 or MSn) analysis and intact glycopeptide assignment. Isotopic recoding is accomplished by metabolic labeling of cell or tissue samples with azide- or alkyne-functionalized sugars, followed by chemical conjugation with a biotin probe bearing a unique isotopic signature.

Some examples of sugar labels are peracetylated N-azidoacetylmannosamine (Ac4ManNAz), which is converted to the corresponding azidosialic acid (SiaNAz), and peracetylated N-azidoacetylgalactosamine (Ac4GalNAz), which is metabolized to label glycans possessing N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) (not provided with kit).

In order to perform isotopic tagging, the kit provides two cleavable IsoTaG probes encoded by zero [M] and two [M + 2] deuterium atoms. Probes with different encoding can be provided by Click Chemistry Tools though custom synthesis. The IsoTaG probe with zero, and that with two deuterium atoms [M, M + 2] can be used in different proportions; 1:1, 1:2, 1:3 and 1:4. Pattern recognition with isotopic ratio of 1:3 showed the highest fidelity.

iso tag img three
Figure 3. Cleavable IsoTaG probe encoded by zero deuterium atoms [M] (R = H) and two deuterium atoms [M+2] (R = D)

Through these probes, a unique isotopic signature is embedded exclusively into the glycopeptides. The isotopic signature serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recorded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group. IsoStamp compares observed and predicted isotopic envelopes to identify chemically tagged species in full-scan mass spectra.

IsoTag has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope-coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for post-translational modification.

Specifications

Enrichment targetAzide-modified proteins
Enrichment TechnologyBiotin-streptavidin based enrichment
Number of Enrichments25
Storage Conditions4C
Shipping ConditionsAmbient temperature

Schematic Workflow

Biotinylation reagents 1442

Documents

Selected References

  1. Woo, C.M., et al. (2017). Development of IsoTaG, a Chemical Glycoproteomics Technique for Profiling Intact N- and O‑Glycopeptides from Whole Cell Proteomess. J. Proteome Res., 16: 1706−18. [PubMed]
  2. Woo, C.M., et al. (2017). Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag). Mol. Cell. Proteomics 17: 764-75. [PubMed]
  3. Goa, G., et al. (2017). Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J. Am. Chem. Soc., 140: 4259−68. [PubMed]
  4. Woo, C.M., et al. (2015). Isotope-targeted glycoproteomics (IsoTaG): a mass-independent platform for intact N- and O-glycopeptide discovery and analysis.Nat Methods., 12: 561-7. [PubMed]
  5. Weerapana, E., et al. (2010). Quantitative reactivity profiling predicts functional cysteines in proteomes. Nature 468: 790−5. [PubMed]

IsoStamp v2

Download
zip archive

The post Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling appeared first on VectorLabs.

]]> Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling https://staging.vectorlabs.com/products/click-go-isotag-kit-for-intact-glycopeptides-profiling/ Tue, 19 Sep 2023 19:50:52 +0000 https://staging.vectorlabs.com/?post_type=product&p=22174 Description

While there has been much interest in profiling the intact glycoproteome, the complexity of glycoproteoforms (and more broadly, all proteoforms) remains challenging to completely define. Mass spectrometry (MS) is commonly employed for characterization of complex proteomic samples. A popular strategy for protein identification is the bottom-up shotgun proteomics approach. In this method, a mixture of proteins is subjected to proteolytic digestion, the resulting peptides are separated by LC and detected by MS, and their parent proteins are inferred from the assigned peptide sequences.

To convert MS data acquired from proteolytic digests into protein identifications, tandem MS can be used to obtain sequence information for individual peptides, followed by comparing an in-silico proteolytic digest of an organism’s proteome. Typically, only the most abundant peptides are selected for fragmentation, whereas data for those peptides in relatively low quantities are not obtained. An inherent problem in shotgun proteomics is identifying proteins of low abundance, such as biomarkers for disease states, against a background of proteins whose concentrations can span up to 12 orders of magnitude.

To address the unique challenges of the global characterization of the intact glycoproteome, a mass-independent chemical glycoproteomics platform, termed isotope targeted glycoproteomics (IsoTag) was developed by the Carolyn Bertozzi group. The platform is comprised of four central components: (i) metabolic labeling with a chemically functionalized glycan, (ii) chemical tagging and enrichment using an isotopic recoding affinity probe, (iii) directed tandem MS, and (iv) targeted glycopeptide assignment.

IsoTaG is performed by isotopic recoding and enrichment of metabolically labeled glycoproteins followed by directed tandem MS (MS2 or MSn) analysis and intact glycopeptide assignment. Isotopic recoding is accomplished by metabolic labeling of cell or tissue samples with azide- or alkyne-functionalized sugars, followed by chemical conjugation with a biotin probe bearing a unique isotopic signature.

Some examples of sugar labels are peracetylated N-azidoacetylmannosamine (Ac4ManNAz), which is converted to the corresponding azidosialic acid (SiaNAz), and peracetylated N-azidoacetylgalactosamine (Ac4GalNAz), which is metabolized to label glycans possessing N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) (not provided with kit).

In order to perform isotopic tagging, the kit provides two cleavable IsoTaG probes encoded by zero [M] and two [M + 2] deuterium atoms. Probes with different encoding can be provided by Click Chemistry Tools though custom synthesis. The IsoTaG probe with zero, and that with two deuterium atoms [M, M + 2] can be used in different proportions; 1:1, 1:2, 1:3 and 1:4. Pattern recognition with isotopic ratio of 1:3 showed the highest fidelity.

Through these probes, a unique isotopic signature is embedded exclusively into the glycopeptides. The isotopic signature serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recorded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group. IsoStamp compares observed and predicted isotopic envelopes to identify chemically tagged species in full-scan mass spectra.

IsoTag has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope-coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for post-translational modification.

Specifications

Enrichment target Alkyne-modified proteins
Enrichment Technology Biotin-streptavidin based enrichment
Number of Enrichments 25
Storage Conditions 4C
Shipping Conditions Ambient temperature

The post Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling appeared first on VectorLabs.

]]>
IsoTaG is a mass-independent chemical glycoproteomics platform that helps to address unique challenges of the global characterization of the intact glycoproteome. In this approach a unique isotopic signature is embedded exclusively into the glycopeptides and it serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recoded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group.

Description

While there has been much interest in profiling the intact glycoproteome, the complexity of glycoproteoforms (and more broadly, all proteoforms) remains challenging to completely define. Mass spectrometry (MS) is commonly employed for characterization of complex proteomic samples. A popular strategy for protein identification is the bottom-up shotgun proteomics approach. In this method, a mixture of proteins is subjected to proteolytic digestion, the resulting peptides are separated by LC and detected by MS, and their parent proteins are inferred from the assigned peptide sequences.

To convert MS data acquired from proteolytic digests into protein identifications, tandem MS can be used to obtain sequence information for individual peptides, followed by comparing an in-silico proteolytic digest of an organism’s proteome. Typically, only the most abundant peptides are selected for fragmentation (Figure 2), whereas data for those peptides in relatively low quantities are not obtained. An inherent problem in shotgun proteomics is identifying proteins of low abundance, such as biomarkers for disease states, against a background of proteins whose concentrations can span up to 12 orders of magnitude.

iso tag img one
Figure 1. Metabolic labeling with a chemically functionalized glycan, chemical tagging and enrichment using an isotopic recoding affinity probe

To address the unique challenges of the global characterization of the intact glycoproteome, a mass-independent chemical glycoproteomics platform, termed isotope targeted glycoproteomics (IsoTag) was developed by the Carolyn Bertozzi group. The platform is comprised of four central components: (i) metabolic labeling with a chemically functionalized glycan, (ii) chemical tagging and enrichment using an isotopic recoding affinity probe, (iii) directed tandem MS, and (iv) targeted glycopeptide assignment (Figure 2).

iso tag img two
Figure 2. Traditional proteomics and Iso-Tag-directed proteomics workflow

IsoTaG is performed by isotopic recoding and enrichment of metabolically labeled glycoproteins followed by directed tandem MS (MS2 or MSn) analysis and intact glycopeptide assignment. Isotopic recoding is accomplished by metabolic labeling of cell or tissue samples with azide- or alkyne-functionalized sugars, followed by chemical conjugation with a biotin probe bearing a unique isotopic signature.

Some examples of sugar labels are peracetylated N-azidoacetylmannosamine (Ac4ManNAz), which is converted to the corresponding azidosialic acid (SiaNAz), and peracetylated N-azidoacetylgalactosamine (Ac4GalNAz), which is metabolized to label glycans possessing N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc) (not provided with kit).

In order to perform isotopic tagging, the kit provides two cleavable IsoTaG probes encoded by zero [M] and two [M + 2] deuterium atoms. Probes with different encoding can be provided by Click Chemistry Tools though custom synthesis. The IsoTaG probe with zero, and that with two deuterium atoms [M, M + 2] can be used in different proportions; 1:1, 1:2, 1:3 and 1:4. Pattern recognition with isotopic ratio of 1:3 showed the highest fidelity.

iso tag img three
Figure 3. Cleavable IsoTaG probe encoded by zero deuterium atoms [M] (R = H) and two deuterium atoms [M+2] (R = D)

Through these probes, a unique isotopic signature is embedded exclusively into the glycopeptides. The isotopic signature serves as a computationally recognizable full-scan MS reporter. A computational algorithm, termed isotopic signature transfer and mass pattern prediction (IsoStamp), for the detection of recorded species in full-scan mass spectra, was also developed by the Carolyn Bertozzi group. IsoStamp compares observed and predicted isotopic envelopes to identify chemically tagged species in full-scan mass spectra.

IsoTag has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope-coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for post-translational modification.

Specifications

Enrichment targetAlkyne-modified proteins
Enrichment TechnologyBiotin-streptavidin based enrichment
Number of Enrichments25
Storage Conditions4C
Shipping ConditionsAmbient temperature

Schematic Workflow

Biotinylation reagents 1442

Documents

Selected References

  1. Woo, C.M., et al. (2017). Development of IsoTaG, a Chemical Glycoproteomics Technique for Profiling Intact N- and O‑Glycopeptides from Whole Cell Proteomess. J. Proteome Res., 16: 1706−18. [PubMed]
  2. Woo, C.M., et al. (2017). Mapping and Quantification of Over 2000 O-linked Glycopeptides in Activated Human T Cells with Isotope-Targeted Glycoproteomics (Isotag). Mol. Cell. Proteomics 17: 764-75. [PubMed]
  3. Goa, G., et al. (2017). Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J. Am. Chem. Soc., 140: 4259−68. [PubMed]
  4. Woo, C.M., et al. (2015). Isotope-targeted glycoproteomics (IsoTaG): a mass-independent platform for intact N- and O-glycopeptide discovery and analysis.Nat Methods., 12: 561-7. [PubMed]
  5. Weerapana, E., et al. (2010). Quantitative reactivity profiling predicts functional cysteines in proteomes. Nature 468: 790−5. [PubMed]

IsoStamp v2

Download
zip archive

The post Click-&-Go® IsoTaG Kit for Intact Glycopeptides Profiling appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-488/ Sun, 30 Apr 2023 03:33:40 +0000 https://staging.vectorlabs.com/?post_type=product&p=13446 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 488
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

 

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 appeared first on VectorLabs.

]]>

Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 488
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 488 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 488 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 488 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-594/ Sun, 30 Apr 2023 03:28:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=13436 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 549
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

 

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

]]>

Resources

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 549
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 594 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 594 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 594 appeared first on VectorLabs.

]]> Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 https://staging.vectorlabs.com/products/glysite-scout-screening-kit-649/ Sun, 30 Apr 2023 03:21:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=13428 Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Recommended Storage Store reagents in original bottles at 2–8 °C. Do not freeze the kit.
Conjugate DyLight 649
Sugar Specificity Mannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

 

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 appeared first on VectorLabs.

]]>

Description

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 is a fully integrated kit for the detection of glycan expression in tissue sections. Included in the kit is a diverse panel of glycan binders (lectins) ideal for the preliminary screening of tissue samples with unknown glycosylation changes with specificity to mannose, complex N-glycan, core O-glycan, fucose, sialic acid, sulfation, GlcNAc, chitin, galactose, and LacNAc.

Validated on human and mouse tissue—including FFPE tissue, across a range of tissue types including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus—this versatile kit is flexible to meet your needs. Glysite Scout Glycan Screening Kits, Immunofluorescence are available with 3 fluorophore options, 488 (GSK-3000), 594 (GSK-2000), and 649 (GSK-1000).

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
Recommended StorageStore reagents in original bottles at 2–8 °C. Do not freeze the kit.
ConjugateDyLight 649
Sugar SpecificityMannose, Galactose, Fucose, Sialic Acid

Kit Components

Kit Contents:

This glycan screening kit is designed to provide the investigator with a panel of eight biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Glysite Scout Glycan Screening Kit, Immunofluorescence 649 contains the following biotinylated lectins:

  • AAL (Aleuria Aurantia)
  • ECL, ECA (Erythrina Cristagalli)
  • GNL (Galanthus Nivalis)
  • Jacalin
  • MAL II (Maackia Amurensis II)
  • PHA-L (Phaseolus Vulgaris Leucoagglutinin)
  • WFA, WFL (Wisteria Fluoribunda)
  • WGA (Wheat Germ Agglutinin)

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI. 

Documents

Product FAQs

Virtually any tissue type and species are suitable for use with a Glysite Scout Glycan Screening Kit, Immunofluorescence, as glycans are ubiquitous in nature and in life. The kit has been validated on human and mouse tissue—including FFPE tissue, across a range of organs, including colon, lung, spleen, kidney, liver, pancreas, testes, heart, bladder, and uterus.

Each of the three Glysite Scout Glycan Screening Kits, Immunofluorescence contain the same lectins but with a different fluorescent streptavidin detection reagent, offering a choice of Dylight™ 488 (GSK-3000), Dylight™ 594 (GSK-2000), or Dylight™ 649 (GSK-1000). This allows users to choose the kit which best matches the capabilities of their fluorescent microscope and also provides flexibility for multiplexing with other fluorescent detection reagents they might already be using. 

VECTASHIELD Vibrance is an antifade medium for immunofluorescence applications with setting properties, which means the coverslip is immobilized after curing and does not require sealing. Additionally, slides can be stored at room temperature, enabling extended archiving time. VECTASHIELD PLUS does not have setting properties and remains molten, and slides must be stored at 2–8°C. Both VECTASHIELD Vibrance and VECTASHIELD PLUS have superior antifade/anti-photobleaching properties across the spectrum and are compatible with commonly used fluorophores. 

The need for an antigen retrieval step in a staining protocol depends on multiple variables, including the target glycan and its location in the specimen, the glycan-binder, the type of tissue, and the method and length of fixation. Investigators should run controls with and without antigen retrieval to determine if this step is needed for their specimen. 

Tissue components such as red blood cells, elastin, and collagen are strongly fluorescent, particularly in FFPE sections, making it difficult to discern between relevant signal and background. Formalin fixation tends to induce a significant amount of fluorescence to a specimen. The Vector TrueVIEW Autofluorescence Quenching Kit provides a dramatic reduction of autofluorescence, allowing a clear view of the desired signal, so it was utilized as needed on the specimens represented in the images. 

If co-labeling with a primary antibody, users should optimize each protocol separately to achieve maximum signal to noise ratios. Once this is complete, the two protocols can be combined, with the Glysite Scout Glycan Screening Kit protocol applied first, followed by the antibody staining protocol. Users should perform the proper controls to demonstrate that the reagents in each protocol are not cross–reacting with each other. 

The Carbo-Free Blocking Solution included in the Glysite Scout Glycan Screening Kit is virtually free of glycoproteins or other glycosylated molecules. This is critical since these kits detect glycans and would therefore bind to blocking reagents which contained glycosylated components. Users should not use other blocking solutions (e.g., milk derivatives, sera) as it may lead to high background. 

Each Glysite Scout Glycan Screening Kit contains enough reagents for approximately 50 tissue samples per lectin, or up to 400 samples if using one lectin per tissue sample (8 lectins x 50 tissue samples). 

The lectins were selected based upon their broad applicability and specificity to frequently cited glycan targets from literature sources, spanning fields of Cancer, Immunology, Metabolism, Inflammation, Virology, and Tumor Microenvironment, among others. They enable detection of all the major glycan motifs for the evaluation of glycan distribution in a target specimen. 

Technical Information

The lectins selected for the Glysite Scout Glycan Screening Kit, Immunofluorescence 649 were specifically chosen to provide detection of all of the major glycan motifs for the evaluation of glycan distribution in a target specimen. Follow our optimized protocol or view our how-to video to learn how you can rapidly explore glycan expression in your tissue section.

In addition to the glycan binders, the kit includes optimized immunofluorescence reagents for glycan detection, including blocking solutions, DyLight™ streptavidin, and VECTASHIELD Vibrance® Antifade Mounting Medium with DAPI.

Legal Information

DyLight is a trademark of Pierce Biotechnology, Inc.

The post Glysite™ Scout Glycan Screening Kit, Immunofluorescence 649 appeared first on VectorLabs.

]]> Lectin Kit II, Biotinylated https://staging.vectorlabs.com/products/lectin-kit-2-biotinylated/ Sat, 29 Apr 2023 18:26:36 +0000 https://staging.vectorlabs.com/?post_type=product&p=13372

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

]]>

Resources

Description

Our lectin screening kits are designed to provide the investigator with a panel of six lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit II (LK-3000, BK-2100, FLK-3100, RLK-3200) consists of 1 mg of the following lectins or lectin conjugates: GSL I, LCA, PHA-E, PHA-L, PSA, Succinylated WGA.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
ConjugateBiotinylated

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of six biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit II consists of 1 mg of the following biotinylated lectins:

  • GSL I (Griffonia (Bandeiraea) simplicifolia lectin I; 2 mg/ml)
  • PSA (Pisum sativum agglutinin; 2 mg/ml)
  • LCA (Lens culinaris agglutinin; 2 mg/ml)
  • PHA-E (Phaseolus vulgaris Erythroagglutinin; 2 mg/ml)
  • PHA-L (Phaseolus vulgaris Leucoagglutinin; 2 mg/ml)
  • Succinylated WGA (Wheat germ agglutinin, succinylated; 2 mg/ml)

Documents

Technical Information

These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative.

The post Lectin Kit II, Biotinylated appeared first on VectorLabs.

]]> Lectin Kit I, Fluorescein https://staging.vectorlabs.com/products/lectin-kit-i-fluorescein-labeled/ Tue, 25 Apr 2023 19:36:21 +0000 https://staging.vectorlabs.com/?post_type=product&p=12015 Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size 1 kit
Applications Immunofluorescence, Glycobiology
Conjugate Fluorescein
Color of Fluorescence Green
Sugar Specificity Mannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

The post Lectin Kit I, Fluorescein appeared first on VectorLabs.

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Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit I (BK-1000, FLK-2100, RLK-2200) consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size1 kit
ApplicationsImmunofluorescence, Glycobiology
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityMannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven fluorescein labeled lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit I consists of 1 mg of the following fluorescein labeled lectin conjugates:

  • Con A (Concanavalin A; 2 mg/ml)
  • SBA (Glycine max (soybean) agglutinin; 2 mg/ml)
  • WGA (Triticum vulgaris (wheat germ) agglutinin; 2 mg/ml)
  • DBA (Dolichos biflorus agglutinin; 2 mg/ml)
  • UEA I (Ulex europaeus agglutinin I; 2 mg/ml)
  • RCA120 (Ricinus communis agglutinin; 2 mg/ml)
  • PNA (Arachis hypogaea (peanut) agglutinin; 2 mg/ml

These lectins are supplied essentially free of unconjugated fluorochrome and inactive lectin.

Fluorescein labeled lectin has an excitation maximum at 495 nm and an emission maximum at 515 nm.

Documents

Citations

Technical Information

Our fluorescein labeled lectins are produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectins, and special conjugation procedures. The lectin conjugates have the appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for each particular lectin. These lectins are supplied essentially free of unconjugated fluorochrome and inactive lectin. Fluorescein labeled lectin has an excitation maximum at 495 nm and an emission maximum at 515 nm.

Accompanying each fluorescent lectin kit is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Lectin screening kits jpg

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]]> Lectin Kit III, Biotinylated https://staging.vectorlabs.com/products/lectin-kit-iii-biotinylated/ Mon, 24 Apr 2023 18:52:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=11397 Specifications
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended Usage Reconstitute lyophilized biotinylated lectins by adding 0.5 ml water. The resulting solution will be: 10 mM HEPES, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, pH 7.5.
Conjugate Biotinylated
Sugar Specificity Galactose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit III consists of 0.5 mg of the following biotinylated lectins:

  • DSL (Datura Stramonium lectin)
  • ECL (Erythrina cristagalli lectin)
  • GSL II (Griffonia (Bandeiraea) simplicifolia lectin II)
  • Jacalin
  • LEL (Lycopersicon esculentum (tomato) lectin)
  • STL (Solanum tuberosum (potatoe) lectin)
  • VVL (Vicia villosa agglutinin)

Technical Information

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit III (BK-3000, FLK-4100) contains the following lectin conjugates: DSL, ECL, GSL II, Jacalin, LEL, STL, VVL, 0.5mg of each

These biotinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Lectin Screening Kits Jpg Lectin-screening-kits Vectorlabs

The post Lectin Kit III, Biotinylated appeared first on VectorLabs.

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Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Recommended UsageReconstitute lyophilized biotinylated lectins by adding 0.5 ml water. The resulting solution will be: 10 mM HEPES, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, pH 7.5.
ConjugateBiotinylated
Sugar SpecificityGalactose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit III consists of 0.5 mg of the following biotinylated lectins:

  • DSL (Datura Stramonium lectin)
  • ECL (Erythrina cristagalli lectin)
  • GSL II (Griffonia (Bandeiraea) simplicifolia lectin II)
  • Jacalin
  • LEL (Lycopersicon esculentum (tomato) lectin)
  • STL (Solanum tuberosum (potatoe) lectin)
  • VVL (Vicia villosa agglutinin)

Documents

Citations

Technical Information

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each Kit III (BK-3000, FLK-4100) contains the following lectin conjugates: DSL, ECL, GSL II, Jacalin, LEL, STL, VVL, 0.5mg of each.

These biotinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Lectin screening kits jpg

The post Lectin Kit III, Biotinylated appeared first on VectorLabs.

]]> Lectin Kit I, Biotinylated https://staging.vectorlabs.com/products/lectin-kit-i-biotinylated/ Mon, 24 Apr 2023 18:43:07 +0000 https://staging.vectorlabs.com/?post_type=product&p=11382 Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each kit consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
Conjugate Biotinylated
Sugar Specificity Mannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit I consists of 1 mg of the following biotinylated lectins:

  • Con A (Concanavalin A; 2 mg/ml)
  • SBA (Glycine max (soybean) agglutinin; 2 mg/ml)
  • WGA (Triticum vulgaris (wheat germ) agglutinin; 2 mg/ml)
  • DBA (Dolichos biflorus agglutinin; 2 mg/ml)
  • UEA I (Ulex europaeus agglutinin I; 2 mg/ml)
  • RCA120 (Ricinus communis agglutinin; 2 mg/ml)
  • PNA (Arachis hypogaea (peanut) agglutinin; 2 mg/ml)

Technical Information

These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Lectin Screening Kits Jpg Lectin-screening-kits Vectorlabs

The post Lectin Kit I, Biotinylated appeared first on VectorLabs.

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Description

Our lectin screening kits are designed to provide the investigator with a panel of seven lectins or lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Each kit consists of 1 mg of the following lectins or lectin conjugates: Con A, DBA, PNA, RCA120 ,SBA, UEA I, WGA.

Specifications

Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
ConjugateBiotinylated
Sugar SpecificityMannose, Galactose, Fucose, N-Acetylgalactosamine, N-Acetylglucosamine

Kit Contents:

This lectin screening kit is designed to provide the investigator with a panel of seven biotinylated lectin conjugates. The lectins have been selected to offer a variety of sugar specificities and are of the same high quality as the reagents offered individually.

Kit I consists of 1 mg of the following biotinylated lectins:

  • Con A (Concanavalin A; 2 mg/ml)
  • SBA (Glycine max (soybean) agglutinin; 2 mg/ml)
  • WGA (Triticum vulgaris (wheat germ) agglutinin; 2 mg/ml)
  • DBA (Dolichos biflorus agglutinin; 2 mg/ml)
  • UEA I (Ulex europaeus agglutinin I; 2 mg/ml)
  • RCA120 (Ricinus communis agglutinin; 2 mg/ml)
  • PNA (Arachis hypogaea (peanut) agglutinin; 2 mg/ml)

Documents

Citations

Technical Information

These bioinylated conjugates are prepared from our affinity-purified lectins and are optimally labeled with biotin. Essentially free of inactive lectin conjugates and containing no free biotin, these biotinylated lectins provide ideal intermediates for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First a biotin-labeled lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.

Lectin screening kits jpg

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