Alkyne Sphinganine a metabolic labeling reagent that contains a terminal alkyne moiety that allows for the subsequent tagging of protein−lipid complexes by click chemistry. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst allowing for the visualization and proteomic profiling of protein−sphingolipid complexes in living cells.

Specifications

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This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst.

E-Cholesterol Alkyne (19-ethynylcholesterol) represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

E-cholesterol Alkyne (19-ethynylcholesterol) is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria.

In summary, alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular
cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

Specifications

The post E-Cholesterol Alkyne appeared first on VectorLabs.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper-containing catalyst.

Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

27-Alkyne cholesterol is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria.

In summary, alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular
cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

Specifications

The post 27-Alkyne Cholesterol appeared first on VectorLabs.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst.

Alkyne cholesterol is accepted by cellular enzymes from different biological species (Brevibacterium, yeast, rat, human) and these enzymes include cholesterol oxidases, hydroxylases, and acyl transferases that generate the expected metabolites in in vitro and in vivo assays. Using fluorescence microscopy, researchers can study the distribution of cholesterol at subcellular resolution, detecting the lipid in the Golgi and at the plasma membrane, but also in the endoplasmic reticulum and mitochondria.

In summary, alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular
cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

Specifications

The post Alkyne Cholesterol appeared first on VectorLabs.

Alkynyl stearic acid is can be used to identify and characterize the post-translational acylation of proteins using the powerful click chemistry, a simple and robust two-step labeling and detection technique. Due to the very small size of the alkyne group and its complete absence in any natural molecules the presence of alkynyl group does not affect the biodistribution of alkynyl palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

Specifications

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Alkynyl myristic acid is can be used to identify and characterize the post-translational myristylated proteins Alkynyl palmitic acid (15-hexadecynoic acid or Palmitic acid (15-yne)) is a modified palmitic acid with an ω-terminal alkyne. Alkynyl palmitic acid often used to identify and characterize the post-translational S-palmitoylation of proteins using the powerful click chemistry (see References).

Specifications

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Alkynyl myristic acid is can be used to identify and characterize the post-translational myristylated proteins using a simple and robust two-step labeling and detection technique. Due to the very small size of the alkyne group and its complete absence in any natural molecules the presence of the alkyne group does not affect the biodistribution of alkynyl myristic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

Specifications

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Azido Myristic Acid (also sold as Click-iT® myristic acid, azide) can be used to identify and characterize post-translationally myristylated proteins with using a simple and robust two-step labeling and detection technique. Due to the very small size of the azide group and its complete absence in any natural molecules the presence of azide group does affect the biodistribution of azido palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

Specifications

The post Azido Myristic Acid appeared first on VectorLabs.

Azido Palmitic Acid (also sold as Click-iT® palmitic acid, azide) can be used to identify and characterize post-translationally palmitylated proteins with using a simple and robust two-step labeling and detection technique. Due to the very small size of the azide group and its complete absence in any natural molecules the presence of azide group does not affect the biodistribution of azido palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

Specifications

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