Nascent Protein Synthesis – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:08:03 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Nascent Protein Synthesis – VectorLabs https://staging.vectorlabs.com 32 32 O-propargyl-puromycine (OPP) https://staging.vectorlabs.com/products/o-propargyl-puromycine-opp/ Tue, 19 Sep 2023 19:32:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=22065 Description

O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Molecular weight 495.54
Chemical composition C24H29N7O5
CAS 1416561-90-4
Solubility DMSO, DMF
Purity >95% (HPLC)
Appearance Off-white to slightly grey crystalline
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

O-propargyl-puromycin (OPP) is an alkyne analog of puromycin that is efficiently incorporated into newly translated proteins, which are rapidly turned over by the proteasome and can be visualized or captured by copper(I)-catalyzed azide-alkyne cycloaddition. Unlike methionine analogs, OPP does not require methionine-free conditions and, uniquely, can be used to label and assay nascent proteins in whole organisms.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Molecular weight495.54
Chemical compositionC24H29N7O5
CAS1416561-90-4
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceOff-white to slightly grey crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Leach, R. W., et al. (2021). Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Nat Chem Biol., 17 (11), 1178-1187. [PubMed]
  2. Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell., 26 (6), 845-861.e12. [PubMed]
  3. Uchiyama, J., et al. (2020). Quantitative nascent proteome profiling by dual pulse labeling with O-propargyl-puromycin and stable isotope labeled amino acids. The Journal of Biochemistry, 10, 1093. [Oxford Academic]
  4. Lui, J., et al. (2012). Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. PNAS, 109, 413-8. [PubMed]
  5. Forester, C.M., et al. (2018). Revealing nascent proteomics in signaling pathways and cell differentiation.PNAS, 115, 2353-8. [PubMed]
  6. Tong, M., et al. (2020). Effective Method for Accurate and Sensitive Quantitation of Rapid Changes of Newly Synthesized Proteins. Anal. Chem., 92(14), 10048-57. [PubMed]

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]]> 4-Azido-L-phenylalanine https://staging.vectorlabs.com/products/4-azido-l-phenylalanine/ Tue, 19 Sep 2023 19:23:37 +0000 https://staging.vectorlabs.com/?post_type=product&p=21867 Description

4-Azido-L-phenylalanine is an unnatural derivative of L-phenylalanine that can be incorporated by recombinant methods into proteins allowing site-specific modification, e.g. by ‘click chemistry’, Staudinger reaction, or by UV-irradiation at 254 nm. The azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size 1 g, 5 g, 25 g
Molecular weight 242.66
Chemical composition C9H10N4O2
CAS 33173-53-4
Solubility Water, DMSO, DMF
Purity >95% (H NMR)
Appearance Off-white to slightly grey crystalline
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

4-Azido-L-phenylalanine is an unnatural derivative of L-phenylalanine that can be incorporated by recombinant methods into proteins allowing site-specific modification, e.g. by ‘click chemistry’, Staudinger reaction, or by UV-irradiation at 254 nm. The azido-modified protein can be detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size1 g, 5 g, 25 g
Molecular weight242.66
Chemical compositionC9H10N4O2
CAS33173-53-4
SolubilityWater, DMSO, DMF
Purity>95% (H NMR)
AppearanceOff-white to slightly grey crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

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]]> L-Homopropargylglycine (HPG) https://staging.vectorlabs.com/products/l-homopropargylglycine-hpg/ Tue, 19 Sep 2023 19:15:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=21753 Description

L-Homopropargylglycine (HPG) is an amino acid analog of methionine that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The alkyne-modified protein is detected with either fluorescent azide or biotin azide. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size 25 mg, 100 mg, 1000 mg
Molecular weight 163.60 (HCl salt)
Chemical composition C6H10ClNO2
CAS 942518-19-6
Solubility Water, DMSO, DMF
Purity >95% (H NMR)
Appearance White crystalline
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

L-Homopropargylglycine (HPG) is an amino acid analog of methionine that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The alkyne-modified protein is detected with either fluorescent azide or biotin azide. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size25 mg, 100 mg, 1000 mg
Molecular weight163.60 (HCl salt)
Chemical compositionC6H10ClNO2
CAS942518-19-6
SolubilityWater, DMSO, DMF
Purity>95% (H NMR)
AppearanceWhite crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Couradeau, E., et al. (2019). Probing the active fraction of soil microbiomes using BONCAT-FACS Nat Commun, 10, 2770-80. [PubMed]

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]]> L-Azidohomoalanine (AHA) https://staging.vectorlabs.com/products/l-azidohomoalanine-aha/ Tue, 19 Sep 2023 19:15:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=21748 Description

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size 25 mg, 100 mg, 1000 mg, 5 g
Molecular weight 180.59 (HCl salt)
Chemical composition C4H9ClN4O2 (HCl salt)
CAS 942518-29-8
Solubility Water, DMSO, DMF
Purity >95% (H NMR)
Appearance White crystalline
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

 

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Description

L-Azidohomoalanine (AHA) is an amino acid analog of methionine that contains a very small modification, specifically an azido moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click ” reaction between an azide and an alkyne or cyclooctyne. The azido-modified protein is detected with either fluorescent alkyne or biotin alkyne. Detection sensitivity with these reagents in 1-D gels and Western blots is in the low femtomole range and compatible with downstream LC-MS⁄MS and MALDI MS analysis.

Specifications

Unit Size25 mg, 100 mg, 1000 mg, 5 g
Molecular weight180.59 (HCl salt)
Chemical compositionC4H9ClN4O2 (HCl salt)
CAS942518-29-8
SolubilityWater, DMSO, DMF
Purity>95% (H NMR)
AppearanceWhite crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Loebel , C., et al. (2022). Metabolic labeling of secreted matrix to investigate cell-material interactions in tissue engineering and mechanobiology. Nat Protoc., 10.1038, Online ahead of print. [PubMed]
  2. Bruna, R. E., et al. (2021). Limitation of phosphate assimilation maintains cytoplasmic magnesium homeostasis. Proc Natl Acad Sci U S A., 118 (11), e2021370118. [PubMed]
  3. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun., 12 (1), 5638. [PubMed]
  4. Kwong, J. Q., et al. (2021). Mitochondrial functional resilience after TFAM ablation in the adult heart. Am J Physiol Cell Physiol., 320 (6), C929-C942. [PubMed]
  5. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]
  6. Coates, H. W. et al. (2021). The mammalian cholesterol synthesis enzyme squalene monooxygenase is proteasomally truncated to a constitutively active form. J Biol Chem., Online ahead of print. [PubMed]
  7. Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell., 26 (6), 845-861.e12. [PubMed]
  8. Smeekens, J. M., et al. (2020). Systematic quantification of the dynamics of newly synthesized proteins unveiling their degradation pathways in human cells. Chem Sci., 11 (13), 3557-3568. [PubMed]
  9. Tao, W.A., et al. (2019). Identification and Quantification of Newly Synthesized Proteins Using Mass Spectrometry‐Based Chemical Proteomics. Mass Spectrometry‐Based Chemical Proteomics. [PubMed]
  10. Dumont, A. A., et al. (2018). ADAP1 limits neonatal cardiomyocyte hypertrophy by reducing integrin cell surface expression. Sci Rep., 8 (1), 13605. [PubMed]

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