Universal Black Quenchers (UBQ) – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:14:12 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Universal Black Quenchers (UBQ) – VectorLabs https://staging.vectorlabs.com 32 32 UBQ-2 NHS Ester https://staging.vectorlabs.com/products/ubq-2-nhs-ester/ Tue, 19 Sep 2023 22:22:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=22908 Description

Universal Black 2 Quencher (UBQ-2, Black Hole Quencher®-2 (BHQ®-2) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 560-670 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes such as Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, and IRDye® 650.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

[caption id="attachment_28201" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima 580 nm
Extinction coefficient 38,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650
Molecular weight 603.59
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

Universal Black 2 Quencher (UBQ-2, Black Hole Quencher®-2 (BHQ®-2) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 560-670 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes such as Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, and IRDye® 650.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima580 nm
Extinction coefficient38,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesCy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650
Molecular weight603.59
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

UB 2

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post UBQ-2 NHS Ester appeared first on VectorLabs.

]]> UBQ-1 NHS Ester https://staging.vectorlabs.com/products/ubq-1-nhs-ester/ Tue, 19 Sep 2023 22:22:18 +0000 https://staging.vectorlabs.com/?post_type=product&p=22904 Description

Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

[caption id="attachment_28201" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima 534 nm
Extinction coefficient 34,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488
Molecular weight 586.58
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post UBQ-1 NHS Ester appeared first on VectorLabs.

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Description

Universal Black 1 Quencher (UBQ-1, Black Hole Quencher®-2 (BHQ®-1) equivalent) is a dark quencher with no native emission due to the polyaromatic-azo backbone. This nonfluorescent acceptor is often the quencher of choice for qPCR probes and for other fluorescence-quenched probe applications. A broad and intense quenching range from 480-580 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with green to yellow emitting dyes such as FAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima534 nm
Extinction coefficient34,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesFAM, Oregon Green®, TET, JOE and HEX, Alexa Fluor® 488 and DyLight® 488
Molecular weight586.58
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

UB 2

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post UBQ-1 NHS Ester appeared first on VectorLabs.

]]> UBQ-3 NHS Ester https://staging.vectorlabs.com/products/ubq-3-nhs-ester/ Tue, 19 Sep 2023 22:22:16 +0000 https://staging.vectorlabs.com/?post_type=product&p=22900 Description

Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

[caption id="attachment_28210" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima 615 nm
Extinction coefficient 40,700 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Cy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight 664.76
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post UBQ-3 NHS Ester appeared first on VectorLabs.

]]>

Description

Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima615 nm
Extinction coefficient40,700 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesCy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight664.76
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

UB 3

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post UBQ-3 NHS Ester appeared first on VectorLabs.

]]> UBQ-3 DBCO https://staging.vectorlabs.com/products/ubq-3-dbco/ Tue, 19 Sep 2023 22:22:14 +0000 https://staging.vectorlabs.com/?post_type=product&p=22896 Description

Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.

UBQ-3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice for labeling intact proteins, lipids and peptides where the presence of cytotoxic copper catalyst is not acceptable.

[caption id="attachment_28210" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima 615 nm
Extinction coefficient 40,700 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Cy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight 958.15
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post UBQ-3 DBCO appeared first on VectorLabs.

]]>

Description

Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.

UBQ-3 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. Often a reagent of choice for labeling intact proteins, lipids and peptides where the presence of cytotoxic copper catalyst is not acceptable.

Specifications

Unit Size5 mg, 25 mg, 100 mg
Reactivity
Primary amine
Abs/Em Maxima615 nm
Extinction coefficient40,700 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesCy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700
Molecular weight958.15
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

UB 3

Documents

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