SY-21 – VectorLabs

SY-21 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:22:12 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-21NHS Ester (QSY®-21 succinimidyl ester equivalent) is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	661 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5.
Molecular weight	815.34
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-21 NHS ester appeared first on VectorLabs.

SY-21 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:18:52 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

SY-21 DBCO (QSY®-21 equivalent) is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.

SY-21 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern SY-21 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Azide
Abs/Em Maxima	661 nm
Extinction coefficient	90,000 cm-1M-1
Solubility	DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5.
Molecular weight	1091.27
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post SY-21 DBCO appeared first on VectorLabs.

SY-21 Azide

Vector Laboratories R&D — Tue, 19 Sep 2023 20:23:05 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

SY-21 Azide is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.

SY-21 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Abs/Em Maxima	661 nm
Extinction Coefficient	90,000 cm-1M-1
Molecular weight	883.06
Spectrally compatible dyes	Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5.
CAS	N/A
Solubility	DMSO, DMF
Appearance	Dark blue solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

The post SY-21 Azide appeared first on VectorLabs.