IR Dyes – VectorLabs

IR 650 DBCO

Vector Laboratories R&D — Tue, 19 Sep 2023 22:34:03 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

IR 650 DBCO (IRDye® 650 DBCO analog) is a water-soluble, bright, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

IR 650 DBCO is a bright, far-red-fluorescent, pH insensitive azide-reactive probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. IR 650 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern IR 650 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Azide, picolyl azide
Abs/Em Maxima	651/668 nm
Extinction coefficient	230,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Cy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight	1193.43
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post IR 650 DBCO appeared first on VectorLabs.

IR 650 Maleimide

Vector Laboratories R&D — Tue, 19 Sep 2023 22:32:47 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

IR 650 Maleimide (IRDye® 650 Maleimide analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Thiol (Cys)
Abs/Em Maxima	651/668 nm
Extinction coefficient	230,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Cy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight	1057.23
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post IR 650 Maleimide appeared first on VectorLabs.

IR 650 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:32:44 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

IR 650 NHS Ester (IRDye® 650 NHS Ester analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5® Dye or Alexa Fluor® 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with IR 650 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. IR 650 conjugates can be used for detection of low abundance targets.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	651/668 nm
Extinction coefficient	230,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Cy5, Alexa Fluor® 647, DyLight® 649,
Molecular weight	1032.18
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post IR 650 NHS ester appeared first on VectorLabs.

IR 750 Maleimide

Vector Laboratories R&D — Tue, 19 Sep 2023 22:32:13 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

IR 750 Maleimide (IRDye® 750 Maleimide analog) is a water-soluble, bright, near-IR fluorescent dye that is spectrally similar to other 750 dyes used for flow cytometry, microscopy and other applications. IR 750 conjugates ideally suited for the 633 nm laser lines or dye-pumped laser. A significant advantage to using long wavelength dyes such as Cy7® Dye or Alexa Fluor® 750 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. IR 750 conjugates are is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	756/776 nm
Extinction coefficient	270,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Cy7, Alexa Fluor® 750, IRDye® 750
Molecular weight	1123.33
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post IR 750 Maleimide appeared first on VectorLabs.

IR 680LT NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:23:14 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

IR 680LT (IRDye® 680LT equivalent) is a bright, water-soluble dye that is structurally very similar to the commonly used Cy®5.5 Dye. The water solubility, brightness, and self-quenching characteristics of this dye were significantly improved by the introduction of two additional sulfo groups. Far-red fluorescent IR 680 conjugates are spectrally similar to other 680 nm dyes that are used for flow cytometry, microscopy and other applications. Proteins can be labeled with IR 680 at high molar ratios without significant self - quenching, leading to brighter conjugates and more sensitive detection.

The NHS ester (succinimidyl ester) is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amine
Abs/Em Maxima	675/694 nm
Extinction coefficient	190,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 680, DyLight® 680, IRDye® 680
Molecular weight	1320.46
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post IR 680LT NHS ester appeared first on VectorLabs.