Carboxyrhodamine Dyes – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:12:28 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Carboxyrhodamine Dyes – VectorLabs https://staging.vectorlabs.com 32 32 5(6)-Carboxyrhodamine 110 NHS Ester https://staging.vectorlabs.com/products/56-carboxyrhodamine-110-nhs-ester/ Tue, 19 Sep 2023 22:49:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=23317 Description

5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.

The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

[caption id="attachment_27819" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity Primary amine
Abs/Em Maxima 502/527 nm
Extinction coefficient 76,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 471.43
Storage Conditions -50°C to -85°C
Shipping Conditions Dry ice

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Description

488nm laser

5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.

The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima502/527 nm
Extinction coefficient76,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight471.43
Storage Conditions-50°C to -85°C
Shipping ConditionsDry ice

Abs/Em Spectra

MB488

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post 5(6)-Carboxyrhodamine 110 NHS Ester appeared first on VectorLabs.

]]> 5-Carboxyrhodamine 110 NHS Ester https://staging.vectorlabs.com/products/5-carboxyrhodamine-110-nhs-ester/ Tue, 19 Sep 2023 22:48:35 +0000 https://staging.vectorlabs.com/?post_type=product&p=23312 Description

5-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

Although the mixed isomers of Carboxyrhodamine 110 NHS Ester preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes.

Abs/Em Spectra

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Reactivity Primary amine
Abs/Em Maxima 502/527 nm
Extinction coefficient 76,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight 471.43
Storage Conditions -50°C to -85°C
Shipping Conditions Dry ice

The post 5-Carboxyrhodamine 110 NHS Ester appeared first on VectorLabs.

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Description

488nm laser

5-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm. For many applications, the dye is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).

Although the mixed isomers of Carboxyrhodamine 110 NHS Ester preferred, routinely used fluorescent dye for labeling proteins, peptides and nucleotides, purification of peptide and nucleotides labeled with 5(6) isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer usually give better resolution in HPLC purification that is often required in the conjugation processes.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amine
Abs/Em Maxima502/527 nm
Extinction coefficient76,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight471.43
Storage Conditions-50°C to -85°C
Shipping ConditionsDry ice

Abs/Em Spectra

MB488

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post 5-Carboxyrhodamine 110 NHS Ester appeared first on VectorLabs.

]]> Carboxyrhodamine 110 Alkyne https://staging.vectorlabs.com/products/carboxyrhodamine-110-alkyne/ Tue, 19 Sep 2023 19:12:50 +0000 https://staging.vectorlabs.com/?post_type=product&p=21699 Description

5(6)-Carboxyrhodamine 110 Alkyne is green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated emperatures.

5(6)-Carboxyrhodamine 110 (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright photostable and pH-insensitive from pH 4 to pH 10 fluorescent dye. This probe can be used with the 488 nm line of argon-ion laser and standard FITC filter set.

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes Fluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight 588.63
CAS N/A
Solubility DMSO, DMF
Purity >95% (HPLC)
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post Carboxyrhodamine 110 Alkyne appeared first on VectorLabs.

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Description

5(6)-Carboxyrhodamine 110 Alkyne is green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC) forming a stable triazole and does not require elevated emperatures.

5(6)-Carboxyrhodamine 110 (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright photostable and pH-insensitive from pH 4 to pH 10 fluorescent dye. This probe can be used with the 488 nm line of argon-ion laser and standard FITC filter set.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight588.63
CASN/A
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

Selected References

  1. Brito, I. L., et al. (2019). Chemoproteomic Profiling of Gut Microbiota-Associated Bile Salt Hydrolase Activity. ACS Cent Sci., 5 (5), 867-873. [PubMed]

The post Carboxyrhodamine 110 Alkyne appeared first on VectorLabs.

]]> Carboxyrhodamine 110 Azide https://staging.vectorlabs.com/products/carboxyrhodamine-110-azide/ Tue, 19 Sep 2023 17:52:32 +0000 https://staging.vectorlabs.com/?post_type=product&p=21400 Description

5(6)-Carboxyrhodamine 110 Azide (also known as Rhodamine Green) is photostable, green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

5(6)-Carboxyrhodamine 110 (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright photostable and pH-insensitive from pH 4 to pH 10 dye. This probe can be used with the 488 nm line of argon-ion laser and standard FITC filter set.

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima 501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes Fluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight 575.59
CAS N/A
Solubility DMSO, DMF
Purity >95% (HPLC)
Appearance Red solid
Storage Conditions -20°C
Shipping Conditions Ambient temperature

The post Carboxyrhodamine 110 Azide appeared first on VectorLabs.

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Description

5(6)-Carboxyrhodamine 110 Azide (also known as Rhodamine Green) is photostable, green-fluorescent probe that reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.

5(6)-Carboxyrhodamine 110 (also known as Rhodamine Green) is the nonsulfonated analog of the Alexa Fluor® 488 dye with excitation/emission maxima ~502/527 nm. Carboxyrhodamine 110 is bright photostable and pH-insensitive from pH 4 to pH 10 dye. This probe can be used with the 488 nm line of argon-ion laser and standard FITC filter set.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
Abs/Em Maxima501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight575.59
CASN/A
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

The post Carboxyrhodamine 110 Azide appeared first on VectorLabs.

]]> Carboxyrhodamine 110 DBCO https://staging.vectorlabs.com/products/carboxyrhodamine-110-dbco/ Tue, 19 Sep 2023 17:24:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=20856 Description

Carboxyrhodamine 110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Carboxyrhodamine110, a non-sulfonated version of Alexa Fluor® 488, is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. Its absorption/emission spectra is a similar to those of DyLight® 488, Alexa Fluor® 488.

Carboxyrhodamine110 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

[caption id="attachment_25358" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 1 mg, 5 mg, 25 mg
Abs/Em Maxima 501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar Dyes Fluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight 880.96
CAS N/A
Solubility DMSO, DMF
Purity >95% (HPLC)
Appearance Red solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

The post Carboxyrhodamine 110 DBCO appeared first on VectorLabs.

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Description

Carboxyrhodamine 110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

Carboxyrhodamine110, a non-sulfonated version of Alexa Fluor® 488, is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. Its absorption/emission spectra is a similar to those of DyLight® 488, Alexa Fluor® 488.

Carboxyrhodamine110 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima501/523 nm
Extinction Coefficient
74,000
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Spectrally Similar DyesFluorescein, Alexa Fluor® 488, DyLight® 488
Molecular weight880.96
CASN/A
SolubilityDMSO, DMF
Purity>95% (HPLC)
AppearanceRed solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF488

Documents

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