CalFluor Dyes – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:07:43 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png CalFluor Dyes – VectorLabs https://staging.vectorlabs.com 32 32 CalFluor 647 Azide https://staging.vectorlabs.com/products/calfluor-647-azide/ Tue, 19 Sep 2023 19:12:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=21689 Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size 1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction 657/674 nm
Spectrally similar dyes
Alexa Fluor® 647, CF® 647, DyLight® 649, Cy5
Molecular weight 933.24
CAS N/A
Solubility Water, DMSO
Purity >95% (HPLC)
Appearance Dark red amorphous solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

The post CalFluor 647 Azide appeared first on VectorLabs.

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CalFluor 647 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 647 Azide is a water-soluble probe, and its excitation band is a good match for the 633 nm or 647 nm laser lines.

Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction657/674 nm
Spectrally similar dyes
Alexa Fluor® 647, CF® 647, DyLight® 649, Cy5
Molecular weight933.24
CASN/A
SolubilityWater, DMSO
Purity>95% (HPLC)
AppearanceDark red amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Selected References

  1. Shieh P., et al. (2015). CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible SpectrumJ. Am. Chem. Soc., 137: 7145−51. [PubMed]
  2. Pawlak, J. B., et al. (2016). The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes. ACS Chem. Biol., 11: 3172−8. [PubMed]
  3. Jun Kit Ow, M., et al. (2020). Super-Resolution Fluorescence Microscopy Reveals Nanoscale Catalytic Heterogeneity on Single Copper Nanowires. ACS Appl. Nano Mater.,, 3, 4, 3163–7. [ACSPublications]

The post CalFluor 647 Azide appeared first on VectorLabs.

]]> CalFluor 580 Azide https://staging.vectorlabs.com/products/calfluor-580-azide/ Tue, 19 Sep 2023 17:59:08 +0000 https://staging.vectorlabs.com/?post_type=product&p=21519 Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size 1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction 591/609 nm
Spectrally similar dyes
Alexa Fluor® 594, CF® 594, DyLight® 594, Texas Red
Molecular weight 878.10
CAS N/A
Solubility Water, DMSO
Purity >90% (HPLC)
Appearance Dark red amorphous solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958. This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

The post CalFluor 580 Azide appeared first on VectorLabs.

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CalFluor 580 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 580 Azide is a water-soluble probe, and its excitation band is a good match for the 568 nm or 591 nm laser lines.

Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction591/609 nm
Spectrally similar dyes
Alexa Fluor® 594, CF® 594, DyLight® 594, Texas Red
Molecular weight878.10
CASN/A
SolubilityWater, DMSO
Purity>90% (HPLC)
AppearanceDark red amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958. This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Selected References

  1. Shieh P., et al. (2015). CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible SpectrumJ. Am. Chem. Soc., 137: 7145−51. [PubMed]
  2. Pawlak, J. B., et al. (2016). The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes. ACS Chem. Biol., 11: 3172−8. [PubMed]

The post CalFluor 580 Azide appeared first on VectorLabs.

]]> CalFluor 555 Azide https://staging.vectorlabs.com/products/calfluor-555-azide/ Tue, 19 Sep 2023 17:59:07 +0000 https://staging.vectorlabs.com/?post_type=product&p=21516 Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size 1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction 561/583 nm
Spectrally similar dyes
Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight 891.09
CAS N/A
Solubility Water, DMSO
Purity >95% (HPLC)
Appearance Dark red amorphous solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

The post CalFluor 555 Azide appeared first on VectorLabs.

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CalFluor 555 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 555 Azide is water-soluble, and its excitation band is a good match for the 532 nm or 555 nm laser lines. Can be used with standard TRITC (tetramethylrhodamine) filter sets.

Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction561/583 nm
Spectrally similar dyes
Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye
Molecular weight891.09
CASN/A
SolubilityWater, DMSO
Purity>95% (HPLC)
AppearanceDark red amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Selected References

  1. Shieh P., et al. (2015). CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible SpectrumJ. Am. Chem. Soc., 137: 7145−51. [PubMed]
  2. Pawlak, J. B., et al. (2016). The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes. ACS Chem. Biol., 11: 3172−8. [PubMed]

The post CalFluor 555 Azide appeared first on VectorLabs.

]]> CalFluor 488 Azide https://staging.vectorlabs.com/products/calfluor-488-azide/ Tue, 19 Sep 2023 17:58:39 +0000 https://staging.vectorlabs.com/?post_type=product&p=21511 Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size 1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction 500/521 nm
Spectrally similar dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF™ 488A dyes, DyLight™488
Molecular weight 835.94
CAS 1798305-98-2
Solubility Water, DMSO
Appearance Yellow to orange amorphous solid
Storage Conditions -20°C. Desiccate
Shipping Conditions Ambient temperature

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

The post CalFluor 488 Azide appeared first on VectorLabs.

]]>

CalFluor 488 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 488 Azide is a water-soluble probe, and its excitation band is a good match for the 488 nm laser lines (with FITC filter sets).

Description

A major shortcoming of the visualization of alkyne-tagged biomolecules with fluorescent azide probes through CuAAC is the need to remove unreacted fluorescent probes. This is particularly problematic when imaging the intracellular environment, tissues of living organisms, or visualizing biomolecules in vivo. Failure to remove all unreacted fluorescent probes is also one of the major contributors to background signal and non-specific binding.

To overcome this shortcoming, Carolyn Bertozzi’s group has designed fluorogenic azide probes that are activated by Cu-catalyzed or metal-free click chemistry. These azide probes are not fluorescent until they react with alkynes. Termed the CalFluors, these probes possess emission maxima that range from green to far-red wavelengths, and enable sensitive biomolecule detection under no-wash conditions. A number of reports show that CalFluor probes are an indispensable tool for sensitive visualization of metabolically labeled molecules (glycans, DNA, RNA, and proteins) in cells, developing zebrafish, and mouse brain tissue slices under no-wash conditions.

Specifications

Unit Size1 mg, 5 mg
Abs/Em Maxima, triazole after click reaction500/521 nm
Spectrally similar dyes
FAM, Alexa Fluor® 488, Atto™ 488, CF™ 488A dyes, DyLight™488
Molecular weight835.94
CAS1798305-98-2
SolubilityWater, DMSO
AppearanceYellow to orange amorphous solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Documents

Disclaimer

CalFluor Azide Probes are covered by U.S. Patent No.: 9,410,958.

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Selected References

  1. Graham, A. J., et al. (2022). Extracellular Electron Transfer Enables Cellular Control of Cu(I)-Catalyzed Alkyne-Azide Cycloaddition. ACS Cent Sci., 8 (2), 246-257. [PubMed]
  2. Shieh P., et al. (2015). CalFluors: A Universal Motif for Fluorogenic Azide Probes across the Visible SpectrumJ. Am. Chem. Soc., 137: 7145−51. [PubMed]
  3. Pawlak, J. B., et al. (2016). The Optimization of Bioorthogonal Epitope Ligation within MHC‑I Complexes. ACS Chem. Biol., 11: 3172−8. [PubMed]

The post CalFluor 488 Azide appeared first on VectorLabs.

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