AZ Dyes – VectorLabs

AZDye 350 Acid

Vector Laboratories R&D — Tue, 19 Sep 2023 23:14:51 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye™ 350 Acid is an amine reactive, non-activated, water-soluble, blue-emitting dye used for derivatizing amine-containing molecules in the presence of activators (e.g. EDC or HATU) through a stable, covalent amide bond. The NHS ester (or succinimidyl ester) of this dye is also available for conjugating AZDye™ 350 to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for producing bright, blue fluorescent conjugates for imaging and flow cytometry. The emission of AZDye™ 350 conjugates is slightly shorter than that of AMCA or AMCA conjugates, reducing AZDye™ 350’s spectral overlap with the emission of fluorescein or Alexa Fluor® 488 dye.

AZDye™ 350 dye structurally is identical to Alexa Fluor® Dye 350.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amines (needs activation)
Abs/Em Maxima	346/445 nm
Extinction coefficient	19,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight	313.27
Storage Conditions	-20°C
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

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AZDye 350 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 23:14:27 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 350 NHS Ester (Alexa Fluor® 350 NHS Ester equivalent) is an amine reactive, water-soluble, blue-emitting dye used to specifically and efficiently modify a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of proteins or antibodies (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

AZDye™ 350 is a moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.

AZDye™ 350 NHS Ester is structurally identical to Alexa Fluor® 350 NHS Ester.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amines
Abs/Em Maxima	346/445 nm
Extinction coefficient	19,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight	410.35
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

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AZDye 430 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 23:13:33 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.

The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Alkynes, cyclooctynes
Abs/Em Maxima	430/539 nm
Extinction coefficient	15,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 430
Molecular weight	600.56
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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AZDye 532 Maleimide

Vector Laboratories R&D — Tue, 19 Sep 2023 23:13:08 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 532 Maleimide is a water-soluble, photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Thiol (Cys)
Abs/Em Maxima	530/555 nm
Extinction coefficient	78,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 532, DyLight® 532
Molecular weight	790.90 (protonated)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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AZDye 532 NHS Ester

Vector Laboratories R&D — Tue, 19 Sep 2023 23:13:06 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye™ 532 NHS Ester is an amine reactive, yellow-emitting dye routinely used to label proteins or antibodies through primary amines (Lys), amine-modified oligonucleotides, and other amine-containing biomolecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.

AZDye™ 532 is a bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Primary amines
Abs/Em Maxima	530/555 nm
Extinction coefficient	81,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 532, DyLight® 532
Molecular weight	723.80
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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AZDye 488 Hydroxylamine

Vector Laboratories R&D — Tue, 19 Sep 2023 22:56:24 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 488 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

AZDye™ 488 (Alexa Fluor® 488 equivalent) is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Aldehyde, ketone
Abs/Em Maxima	494/517 nm
Extinction coefficient	73,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 488, CF™ 488
Molecular weight	606.58 (protonated)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

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AZDye 488 Hydrazide

Vector Laboratories R&D — Tue, 19 Sep 2023 22:56:22 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye™ 488 Hydrazide is a bright, green-fluorescent dye that often used for labeling aldehydes or ketones in polysaccharides or glycoproteins. AZDye™ 488 Hydrazide is useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores.

AZDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AZDye™ 488 dyes are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Reactivity	Aldehyde, ketone,
Abs/Em Maxima	494/517 nm
Extinction coefficient	73,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488
Molecular weight	548.50 (protonated)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

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AZDye 546 NHS ester

Vector Laboratories R&D — Tue, 19 Sep 2023 22:47:34 +0000

Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Specifications

Unit Size	5 mg, 25 mg, 100 mg
Reactivity	Primary amines
Abs/Em Maxima	554/570 nm
Extinction coefficient	112,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 546, TAMRA, CF™ 543, MB™ 543
Molecular weight	1159.6
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

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AZDye 568 Cadaverine

Vector Laboratories R&D — Tue, 19 Sep 2023 22:47:32 +0000

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye™ 568 Cadaverine is a carbonyl reactive building block used to modify carboxylic groups in the presence of activators (e.g. EDC, or DCC) or activated esters (e.g. NHS esters) through a stable amide bond. It also can be employed as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety.

AZDye™ 568 is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

Specifications

Unit Size	1 mg, 5 mg, 25 mg, 100 mg
Reactivity	Aldehyde, carboxylic acid, ketone
Abs/Em Maxima	578/602 nm
Extinction coefficient	93,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, CF™ 568
Molecular weight	892.92 (TFA salt)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Documents

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AZDye 568 Hydroxylamine

Vector Laboratories R&D — Tue, 19 Sep 2023 22:47:31 +0000

Description
Specifications
Abs/Em Spectra
Protein Labeling Calculator
Documents

Description

AZDye 488 Hydroxylamine (aminooxy) reacts with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. AZDye 568 Hydroxlamine often used as membrane-impermeant, aldehyde-fixable cell tracers as well as reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Reactivity	Aldehyde, ketone
Abs/Em Maxima	578/602 nm
Extinction coefficient	93,000 cm-1M-1
Solubility	Water, DMSO, DMF
Spectrally similar dyes	Alexa Fluor® 568, CF™ 568
Molecular weight	766.84 (protonated)
Storage Conditions	-20°C.
Shipping Conditions	Ambient temperature

Abs/Em Spectra

Protein Labeling Calculator

Protein name

Protein molecular weight (daltons)

Protein concentration (mg/mL)

Volume of protein to be labeled (µL)

Enter only one — either volume or amount of protein to be labeled

Amount of protein to be labeled (mg)

Molecular weight of labeling reagent (Da)

Amount of labeling reagent used (mg)

Maximum concentration of DMSO (%) Leave blank or 0 if not used

Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Volume of anhydrous DMSO added (µL)

Molar equivalents of labeling reagent used

Volume of DMSO required to make a stock solution (µL)

Volume of protein to be labeled (µL)

Amount of stock labeling reagent to be added (µL)

Amount of DMSO in labeling reaction (%)

Documents

The post AZDye 568 Hydroxylamine appeared first on VectorLabs.