AMCA Dyes – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:12:35 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png AMCA Dyes – VectorLabs https://staging.vectorlabs.com 32 32 AMCA Acid https://staging.vectorlabs.com/products/amca-acid/ Tue, 19 Sep 2023 23:14:26 +0000 https://staging.vectorlabs.com/?post_type=product&p=23453 Description

AMCA Acid (7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. Often used as contrasting probes for double- and triple-labeling inimmunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA Acid is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AMCA Acid is also often used for control experiments, and for calibration.

[caption id="attachment_27859" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 25 mg, 100 mg, 1000 mg
Reactivity Primary amines (needs activation)
Abs/Em Maxima 345/450 nm
Extinction coefficient 19,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight 233.27
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

350nm laser 1 1

AMCA Acid (7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. Often used as contrasting probes for double- and triple-labeling inimmunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA Acid is a reagent of choice for the preparation of custom activated esters that often are not commercially available. Examples of such activated esters include sulfo-NHS, TFP (2,3,5,6-Tetrafluorophenol), STP (4-Sulfo-2,3,5,6-Tetrafluorophenol, Sodium Salt). Another common application for non-activated carboxylic acid is peptide modification during solid phase synthesis, which usually requires in-situ activation with peptide coupling regents, for example HATU. AMCA Acid is also often used for control experiments, and for calibration.

Specifications

Unit Size25 mg, 100 mg, 1000 mg
ReactivityPrimary amines (needs activation)
Abs/Em Maxima345/450 nm
Extinction coefficient19,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 350, AMCA, DyLight® 350
Molecular weight233.27
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AMCA abs em

Documents

The post AMCA Acid appeared first on VectorLabs.

]]> AMCA NHS Ester https://staging.vectorlabs.com/products/amca-nhs-ester/ Tue, 19 Sep 2023 23:14:02 +0000 https://staging.vectorlabs.com/?post_type=product&p=23447 Description

AMCA NHS Ester (succinimydyl-7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

[caption id="attachment_27859" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 5 mg, 25 mg, 100 mg
Reactivity Primary amines
Abs/Em Maxima 345/450 nm
Extinction coefficient 19,000 cm-1M-1
Solubility DMSO, DMF
Spectrally similar dyes Alexa Fluor® 350, AMCA, DyLight® 350
Molecular weight 330.30
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post AMCA NHS Ester appeared first on VectorLabs.

]]>

Description

350nm laser 1 1

AMCA NHS Ester (succinimydyl-7-amino-4-methylcoumarin-3-acetic acid) is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. Often used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke’s shift and resistance to photobleaching.

AMCA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.

Specifications

Unit Size5 mg, 25 mg, 100 mg
ReactivityPrimary amines
Abs/Em Maxima345/450 nm
Extinction coefficient19,000 cm-1M-1
SolubilityDMSO, DMF
Spectrally similar dyesAlexa Fluor® 350, AMCA, DyLight® 350
Molecular weight330.30
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AMCA abs em

Documents

The post AMCA NHS Ester appeared first on VectorLabs.

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