5-Carboxy-X-Rhodamine (ROX) Dyes – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:12:57 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png 5-Carboxy-X-Rhodamine (ROX) Dyes – VectorLabs https://staging.vectorlabs.com 32 32 5-ROX (5-Carboxy-X-Rhodamine) https://staging.vectorlabs.com/products/5-rox-5-carboxy-x-rhodamine/ Tue, 19 Sep 2023 22:50:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=23343 Description

The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Often a dye of choice for fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.

[caption id="attachment_27969" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 25 mg, 100 mg, 1000 mg
Reactivity Primary amines
Abs/Em Maxima 570/591 nm
Extinction coefficient 93,000 cm-1M-1
Solubility DMSO, DMF, MeOH
Spectrally similar dyes Alexa Fluor® 568, TAMRA, CF™ 568
CAS 216699-35-3
Molecular weight 534.61
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

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Description

The carboxylic acid of 5-ROX is used for oligonucleotide labeling and automated DNA sequencing applications. Often a dye of choice for fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.

Specifications

Unit Size25 mg, 100 mg, 1000 mg
ReactivityPrimary amines
Abs/Em Maxima570/591 nm
Extinction coefficient93,000 cm-1M-1
SolubilityDMSO, DMF, MeOH
Spectrally similar dyesAlexa Fluor® 568, TAMRA, CF™ 568
CAS216699-35-3
Molecular weight534.61
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

ROX full

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post 5-ROX (5-Carboxy-X-Rhodamine) appeared first on VectorLabs.

]]> 5-ROX NHS Ester https://staging.vectorlabs.com/products/5-rox-nhs-ester/ Tue, 19 Sep 2023 22:49:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=23339 Description

5-Carboxy-X-rhodamine N-succinimidyl ester (5-ROX NHS Ester) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.

[caption id="attachment_27969" align="alignnone" width="1000"] Abs/Em Spectra[/caption]

Specifications

Unit Size 25 mg, 100 mg, 1000 mg
Reactivity Primary amines
Abs/Em Maxima 582/606 nm
Extinction coefficient 93,000 cm-1M-1
Solubility DMSO, DMF, MeOH
Spectrally similar dyes Alexa Fluor® 568, TAMRA, CF™ 568
CAS 209734-74-7
Molecular weight 631.69
Storage Conditions -20°C.
Shipping Conditions Ambient temperature

The post 5-ROX NHS Ester appeared first on VectorLabs.

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Description

5-Carboxy-X-rhodamine N-succinimidyl ester (5-ROX NHS Ester) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.

Specifications

Unit Size25 mg, 100 mg, 1000 mg
ReactivityPrimary amines
Abs/Em Maxima582/606 nm
Extinction coefficient93,000 cm-1M-1
SolubilityDMSO, DMF, MeOH
Spectrally similar dyesAlexa Fluor® 568, TAMRA, CF™ 568
CAS209734-74-7
Molecular weight631.69
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

ROX full

Protein Labeling Calculator

Enter only one — either volume or amount of protein to be labeled
Enter only one — either maximum concentration of DMSO or volume DMSO added to label

Documents

The post 5-ROX NHS Ester appeared first on VectorLabs.

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