Fluorescent Dye Labeling Kits – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:15:15 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Fluorescent Dye Labeling Kits – VectorLabs https://staging.vectorlabs.com 32 32 Click-&-Go® Plus 647 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-647-imaging-kit/ Tue, 19 Sep 2023 19:23:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=21889 Description

Click-&-Go® Plus 647 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with far-red fluorescent AZDye 647 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 647
Abs/Em Maxima
648/674 nm
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

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Description

Click-&-Go® Plus 647 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with far-red fluorescent AZDye 647 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 647
Abs/Em Maxima
648/674 nm
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]

The post Click-&-Go® Plus 647 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 594 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-594-imaging-kit/ Tue, 19 Sep 2023 19:23:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=21887 Description

Click-&-Go® Plus 594 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 594 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 594
Abs/Em Maxima
590/617 nm
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
561 nm or 594 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 594 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 594 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 594 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 594
Abs/Em Maxima
590/617 nm
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
561 nm or 594 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]

The post Click-&-Go® Plus 594 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 568 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-568-imaging-kit/ Tue, 19 Sep 2023 19:23:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=21883 Description

Click-&-Go® Plus 568 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 568 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 568
Abs/Em Maxima
578/602 nm
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 568 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 568 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 568 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 568
Abs/Em Maxima
578/602 nm
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 568 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 532 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-532-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21880 Description

Click-&-Go® Plus 532 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with yellow-fluorescent AZDye 532 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 532
Abs/Em Maxima
532/554 nm
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 532 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 532 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with yellow-fluorescent AZDye 532 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 532
Abs/Em Maxima
532/554 nm
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 532 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 546 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-546-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21881 Description

Click-&-Go® Plus 546 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 546 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 546
Abs/Em Maxima
543/563
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 546 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 546 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 546 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 546
Abs/Em Maxima
543/563
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 546 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 555 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-555-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21882 Description

Click-&-Go® Plus 555 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 555 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 555
Abs/Em Maxima
555/572 nm
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 555 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 555 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 555 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 555
Abs/Em Maxima
555/572 nm
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 555 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 488 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-488-imaging-kit/ Tue, 19 Sep 2023 19:23:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=21879 Description

Click-&-Go® Plus 488 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with green-fluorescent AZDye 488 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 488
Abs/Em Maxima
494/517 nm
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 488 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 488 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with green-fluorescent AZDye 488 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 488
Abs/Em Maxima
494/517 nm
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 488 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 405 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-405-imaging-kit/ Tue, 19 Sep 2023 19:23:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=21878 Description

Click-&-Go® Plus 405 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with blue-fluorescent AZDye 405 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 405
Abs/Em Maxima
402/424
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 405 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 405 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with blue-fluorescent AZDye 405 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 405
Abs/Em Maxima
402/424
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 405 Imaging Kit appeared first on VectorLabs.

]]> R-PE Antibody Conjugation Kit https://staging.vectorlabs.com/products/r-pe-antibody-conjugation-kit/ Wed, 03 May 2023 20:50:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=14887 Description

The R-PE Antibody Conjugation Kit is a flexible alternative to the R-PE Antibody All-in-One™ Conjugation Kit. Each kit contains sufficient material for two reactions of up to 1.3 mg antibody each. Based on SoluLINK® bioconjugation technology, the reactions are rapid and high-yielding, converting nearly 100% of antibody to PE conjugate. This kit is ideal for applications where purification to remove excess R-PE is either not required, or purification will be carried out by chromatographic methods (such as size exclusion FPLC).

Specifications

 
Conjugation Targets Antibody
Label/Modifier Type R-PE
Reactivity Amine
Recommended Storage 2° – 8° C — Do Not Freeze
Applications Antibody Labeling

Kit Components

  • S-HyNic (2 x 1.0 mg)
  • 4FB-R-PE (2 x 3.2 mg)
  • 10X Modification Buffer (1.5 mL)
  • 10X Conjugation Buffer (1.5 mL)
  • 10X TurboLINK™ Catalyst Buffer (1.5 mL)
  • Anhydrous DMF (1.5 mL)
  • 0.5 mL Thermo Scientific™ Zeba™ Column (4)
  • 2 mL Collection Tube (12)
  • 2-Sulfobenzaldehyde, 2-SB (10 mg)
  • 10X MES Buffer (1.5 mL)
  • 2 mL Zeba™ Column (2)

The post R-PE Antibody Conjugation Kit appeared first on VectorLabs.

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Description

The R-PE Antibody Conjugation Kit is a flexible alternative to the R-PE Antibody All-in-One™ Conjugation Kit. Each kit contains sufficient material for two reactions of up to 1.3 mg antibody each. Based on SoluLINK® bioconjugation technology, the reactions are rapid and high-yielding, converting nearly 100% of antibody to PE conjugate. This kit is ideal for applications where purification to remove excess R-PE is either not required, or purification will be carried out by chromatographic methods (such as size exclusion FPLC).

Specifications

 
Conjugation TargetsAntibody
Label/Modifier TypeR-PE
ReactivityAmine
Recommended Storage2° – 8° C — Do Not Freeze
ApplicationsAntibody Labeling

Kit Components

  • S-HyNic (2 x 1.0 mg)
  • 4FB-R-PE (2 x 3.2 mg)
  • 10X Modification Buffer (1.5 mL)
  • 10X Conjugation Buffer (1.5 mL)
  • 10X TurboLINK™ Catalyst Buffer (1.5 mL)
  • Anhydrous DMF (1.5 mL)
  • 0.5 mL Thermo Scientific™ Zeba™ Column (4)
  • 2 mL Collection Tube (12)
  • 2-Sulfobenzaldehyde, 2-SB (10 mg)
  • 10X MES Buffer (1.5 mL)
  • 2 mL Zeba™ Column (2

Documents

Technical Information

R-PE-Antibody Conjugation Kit Using the SoluLINK bioconjugation Technology

The SoluLINK technology can be used to more easily and efficiently prepare R-PE-antibody conjugates as compared to alternative (maleimide/thiol-based) protocols. Other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. The SoluLINK bioconjugation technology, however, gently incorporates HyNic moieties on the intact antibody.

This technology is superior to the maleimide/thiol-based method in the following ways:

  • Efficiency: Greater than 95% of antibody is converted to conjugate and only 1-1.5 molar equivalents of R-PE is required per mole of antibody to produce the conjugate.
  • Ease of purification: In most cases, the percent conversion of free antibody to conjugate is >95. Therefore it is only necessary to remove the excess R-PE to obtain a purified conjugate and in many applications, purification is not necessary.
  • Reaction Control: The level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic modification on the antibody. This is particularly helpful in some applications, such as flow cytometry, where a heterodimer product is preferred.

 

Introduction to SoluLINK Bioconjugation Technology

The SoluLINK bioconjugation technology is based on the formation of a stable bond formed by the reaction of an aromatic hydrazine and an aromatic aldehyde (Figure 1). S-HyNic is an amino-reactive modification reagent that directly converts amino groups on biomolecules and surfaces to HyNic groups. S-HyNic 1 (succinimidyl 6-hydrazinonicotinate acetone hydrazone, SANH) is used to incorporate aromatic hydrazine linkers on biomolecules. S- 4FB 2 (succinimidyl 4-formylbenzoate, SFB) is used to convert amino groups to aromatic aldehydes (4-formylbenzamide (4FB) groups). Addition of a HyNic-modified biomolecule to a 4FB-modified R-PE leads to the formation of the conjugate via a bis-arylhydrazone bond. The bis-aryl hydrazone bond is stable to 92°C and pH 2.0-10.0. The recommended pH for antibody conjugation is 6.0.

P 9002 F1 jpg
Figure 1: Schematic of the two-step protocol to prepare R-PE-antibody conjugates. Step 1 is the modification of antibody with S-HyNic to prepare HyNic-antibody. Step 2 is the conjugation of HyNic-antibody to 4FB-R-PE by mixing in 100 mM phosphate, 150 mM NaCl, 10 mM aniline, pH 6.0.

Advantages of the HyNic-4FB conjugation couple include:

  • High Yielding: Yields of conjugate are routinely 40-60%, based on starting protein.
  • Efficient: Dirksen et al* discovered that aniline catalyzes Schiff’s base formation. This is especially effective for large biomolecule conjugations. In the case of antibody-protein conjugations, the addition of 10 mM aniline to the reaction mixture converts >95% of the antibody to conjugate in ~2 hours using 1-1.5 mole equivalents of second protein as strikingly demonstrated in this product.
  • Stability: The bis-arylhydrazone conjugate bond is stable to 92°C and pH 2.0-10.0.
  • Mild reaction conditions: These mild conditions do not cause antibody denaturation: Unlike thiol-based conjugation protocols where reducing reagents are required that can compromise the activity of proteins by cleaving disulfide bonds, the HyNic-4FB conjugation couple leaves disulfide bonds intact. No metals, oxidation or reducing reagents are required.
  • Spectrophotometric Traceability: The HyNic-4FB conjugate bond is chromophoric, it absorbs at 354 nm and has a molar extinction coefficient of 29,000 L/(mol*cm). This allows,
    • Real time spectrophotometric monitoring of a conjugate reaction.
    • The ability to visualize the conjugate during chromatographic purification using a UV or photodiode array detector.
    • Quantification of conjugation.

There are three crucial requirements that must be fulfilled for a reproducibly successful preparation of an R-PE Antibody conjugate using the SoluLINK bioconjugation technology:

  • Antibody buffer exchange: Prior to modification, the starting antibody must be completely exchanged into Modification Buffer, pH 8.0.
  • Minimum level of HyNic-modification: The HyNic-antibody molar substitution ratio (MSR) must be >3.5 as determined by a colorimetric assay.
  • HyNic Concentration: The final concentration of the HyNic-antibody in the conjugation reaction must be >1.5 mg/mL.

 

References

*Dirksen, A., et al. Nucleophilic catalysis of hydrazone formation and transimination: implications for dynamic covalent chemistry. J Am Chem Soc, 2006. 128(49): p. 15602‐3.

Legal Information

SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

Zeba and Thermo Scientific are trademarks of Thermo Fisher Scientific Inc.

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