AzDye Labeling Kits – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:07:18 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png AzDye Labeling Kits – VectorLabs https://staging.vectorlabs.com 32 32 Click-&-Go® Plus 647 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-647-imaging-kit/ Tue, 19 Sep 2023 19:23:48 +0000 https://staging.vectorlabs.com/?post_type=product&p=21889 Description

Click-&-Go® Plus 647 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with far-red fluorescent AZDye 647 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 647
Abs/Em Maxima
648/674 nm
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 647 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 647 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with far-red fluorescent AZDye 647 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 647
Abs/Em Maxima
648/674 nm
Flow Cytometry Laser Line
633 nm or 647 nm
Microscopy Laser Line
633 nm or 647 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]

The post Click-&-Go® Plus 647 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 594 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-594-imaging-kit/ Tue, 19 Sep 2023 19:23:47 +0000 https://staging.vectorlabs.com/?post_type=product&p=21887 Description

Click-&-Go® Plus 594 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 594 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 594
Abs/Em Maxima
590/617 nm
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
561 nm or 594 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 594 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 594 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 594 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 594
Abs/Em Maxima
590/617 nm
Flow Cytometry Laser Line
561 nm or 594 nm
Microscopy Laser Line
561 nm or 594 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]
  4. Knutson, S.D.,et al. (2020). Chemical Profiling of A‐to‐I RNA Editing Using a Click‐Compatible Phenylacrylamide Chem. Eur.J., 26, 9874-78. [PubMed]

The post Click-&-Go® Plus 594 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 568 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-568-imaging-kit/ Tue, 19 Sep 2023 19:23:46 +0000 https://staging.vectorlabs.com/?post_type=product&p=21883 Description

Click-&-Go® Plus 568 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 568 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 568
Abs/Em Maxima
578/602 nm
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 568 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 568 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with red-fluorescent AZDye 568 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 568
Abs/Em Maxima
578/602 nm
Flow Cytometry Laser Line
532 nm or 568 nm
Microscopy Laser Line
532 nm or 568 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 568 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 532 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-532-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21880 Description

Click-&-Go® Plus 532 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with yellow-fluorescent AZDye 532 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 532
Abs/Em Maxima
532/554 nm
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 532 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 532 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with yellow-fluorescent AZDye 532 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 532
Abs/Em Maxima
532/554 nm
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
532 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 532 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 546 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-546-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21881 Description

Click-&-Go® Plus 546 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 546 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 546
Abs/Em Maxima
543/563
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 546 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 546 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 546 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 546
Abs/Em Maxima
543/563
Flow Cytometry Laser Line
532 nm
Microscopy Laser Line
543 nm or 546 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 546 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 555 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-555-imaging-kit/ Tue, 19 Sep 2023 19:23:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=21882 Description

Click-&-Go® Plus 555 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 555 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 555
Abs/Em Maxima
555/572 nm
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 555 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 555 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with orange-fluorescent AZDye 555 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 555
Abs/Em Maxima
555/572 nm
Flow Cytometry Laser Line
532 nm or 555 nm
Microscopy Laser Line
532 nm or 555 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 555 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 488 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-488-imaging-kit/ Tue, 19 Sep 2023 19:23:44 +0000 https://staging.vectorlabs.com/?post_type=product&p=21879 Description

Click-&-Go® Plus 488 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with green-fluorescent AZDye 488 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 488
Abs/Em Maxima
494/517 nm
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 488 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 488 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with green-fluorescent AZDye 488 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 488
Abs/Em Maxima
494/517 nm
Flow Cytometry Laser Line
488 nm
Microscopy Laser Line
488 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 488 Imaging Kit appeared first on VectorLabs.

]]> Click-&-Go® Plus 405 Imaging Kit https://staging.vectorlabs.com/products/click-go-plus-405-imaging-kit/ Tue, 19 Sep 2023 19:23:43 +0000 https://staging.vectorlabs.com/?post_type=product&p=21878 Description

Click-&-Go® Plus 405 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with blue-fluorescent AZDye 405 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size 1 kit
Dye AZDye 405
Abs/Em Maxima
402/424
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Number of Reactions 30
Storage Conditions 4 C
Shipping Conditions Ambient temperature

The post Click-&-Go® Plus 405 Imaging Kit appeared first on VectorLabs.

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Description

Click-&-Go® Plus 405 Imaging Kit is an all-inclusive kit optimized for imaging alkyne-tagged biopolymers with blue-fluorescent AZDye 405 Azide Plus. Azide Plus reagents contain a complete copper-chelating system in their structure, allowing for the formation of strong, active copper complexes that act simultaneously as both reactant and catalyst in the CuAAC reaction. This azide-copper complex reacts almost instantaneously with alkynes under diluted conditions. This unprecedented reactivity in the CuAAC reaction is of special value for the detection of low abundance targets, improving biocompatibility, and is also valuable for any other application where greatly improved S/N ratio is highly desired. Sufficient materials is supplied to perform at least 30 cell or tissue assays based on a total reaction volume of 500 µL.

Specifications

Unit Size1 kit
DyeAZDye 405
Abs/Em Maxima
402/424
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Number of Reactions30
Storage Conditions4 C
Shipping ConditionsAmbient temperature

Documents

Selected References

  1. Bevilacqua, V., et al. (2014). Copper-Chelating Azides for Efficient Click Conjugation Reactions in Complex Media. Angew. Chem. Int. Ed,., 53, 5872-6. [PubMed]
  2. Su, Y., et al. (2016). All-In-One Azides: Empowered Click Reaction for in vivo Labeling and Imaging of Biomolecules hem. Commun.,, 10, 62185-8. [PubMed]
  3. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]
  4. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]
  5. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]

The post Click-&-Go® Plus 405 Imaging Kit appeared first on VectorLabs.

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