Biotin and Digoxigenin Labeling Reagents – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:21:49 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin and Digoxigenin Labeling Reagents – VectorLabs https://staging.vectorlabs.com 32 32 Biotin-dPEG®₁₂-DBCO https://staging.vectorlabs.com/products/biotin-dpeg12-dbco/ Sat, 24 Feb 2024 02:18:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=39836 Biotin-dPEG®12-DBCO, product number QBD-11811, is a click chemistry biotinylation reagent built on a medium-length (40 atoms, 46.3 Å) single molecular weight PEG linker with a discrete chain length (dPEG®) and functionalized with dibenzylcyclooctyne (DBCO) for strain-promoted azide-alkyne cycloaddition (SPAAC), also known as "copper-free click chemistry." The DBCO functional group reacts with any available azide moiety on a target molecule via SPAAC. The amphiphilic dPEG® spacer imparts water solubility to the biotin label, reducing non-specific hydrophobic interactions and reducing or eliminating concerns over aggregation and precipitation due to biotin's well-known hydrophobicity. Because the hydrophilic dPEG® linker is longer than the classic, hydrophobic LC-LC-biotin, the biotin moiety has potentially better access to the binding pockets of avidin and streptavidin. Peptide and protein modification can be used to introduce suitable azide partners for this biotin labeling compound.

Specifications

Unit Size 10 mg, 25 mg, 1000 mg
Molecular Weight 1102.34; single compound
Chemical formula C₅₅H₈₃N₅O₁₆S
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 40 atoms and 46.3 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, Methanol, DMF, DMAC, or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Biotin-dPEG®12-DBCO, product number QBD-11811, is a click chemistry biotinylation reagent built on a medium-length (40 atoms, 46.3 Å) single molecular weight PEG linker with a discrete chain length (dPEG®) and functionalized with dibenzylcyclooctyne (DBCO) for strain-promoted azide-alkyne cycloaddition (SPAAC), also known as “copper-free click chemistry.” The DBCO functional group reacts with any available azide moiety on a target molecule via SPAAC. The amphiphilic dPEG® spacer imparts water solubility to the biotin label, reducing non-specific hydrophobic interactions and reducing or eliminating concerns over aggregation and precipitation due to biotin’s well-known hydrophobicity. Because the hydrophilic dPEG® linker is longer than the classic, hydrophobic LC-LC-biotin, the biotin moiety has potentially better access to the binding pockets of avidin and streptavidin. Peptide and protein modification can be used to introduce suitable azide partners for this biotin labeling compound.

Specifications

Unit Size10 mg, 25 mg, 1000 mg
Molecular Weight1102.34; single compound
Chemical formulaC₅₅H₈₃N₅O₁₆S
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 40 atoms and 46.3 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, Methanol, DMF, DMAC, or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0). Specifically see pp. 726-729 in his Chapter 18 on discrete PEG compounds for pegylation applications.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See chapter 18, Discrete PEG Reagents, pp.787-821, for a full overview of the dPEG® products.

Engineering the Orientation, Density, and Flexibility of Single-Domain Antibodies on Nanoparticles To Improve Cell Targeting. Ken W. Yong, Daniel Yuen, Moore Z. Chen, Angus P. R. Johnston. ACS Appl. Mater. Interfaces 2020, 12, 5, 5593-5600. January 9, 2020. DOI: 10.1021/acsami.9b20993

The post Biotin-dPEG®₁₂-DBCO appeared first on VectorLabs.

]]> Biotin-dPEG®₁₁-oxyamine. HCl https://staging.vectorlabs.com/products/biotin-dpeg11-oxyamine-hcl/ Sat, 24 Feb 2024 02:15:00 +0000 https://staging.vectorlabs.com/?post_type=product&p=37488 Biotin-dPEG®11-oxyamine·HCl, product number QBD-11100, consists of a medium-length (38 atoms, 44.9 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized at each end with biotin and an oxyamine group (as the HCl salt), respctively. This PEG biotin compound reacts with aldehydes and ketones to forms stable oxime bonds. Using this biotinylation reagent permits biotin labeling of the carbohydrate coats of glycoproteins after the introduction of aldehydes and ketones on the carbohydrate coat using enzymatic (for example, galactose oxidase) or mild chemical reaction conditions such as sodium periodate.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or surfaces such as glass or gold. Bioconjugation research and product development and numerous clinical assays use biotinylated molecules. The amphiphilic dPEG® linker of Biotin-dPEG®11-oxyamine·HCl imparts excellent water solubility to biotin, which is usually poorly soluble in water and causes non-specific hydrophobic interactions when conjugated to biomolecules.

Some commercially available biotin-aminooxy products use either no spacer or a hydrophobic alkyl chain as a spacer between biotin and the aminooxy moiety. Such products have poor solubility in water and must be dissolved in a dry organic solvent such as dimethyl sulfoxide (DMSO) immediately before use, which can be problematic under some conditions. If used on proteins, these hydrophobic products may trigger aggregation and precipitation of the biotinylated molecule. Even if aggregation and precipitation do not occur, the hydrophobic spacer can contribute to non-specific binding. Because it is amphiphilic, Biotin-dPEG®11-oxyamine·HCl can be dissolved in water or aqueous buffer and used directly in a conjugation reaction. QBD-11100 will not cause aggregation or precipitation of the biotinylated molecule. Moreover, the hydrophilicity of the dPEG® linker reduces or eliminates non-specific binding.

An aminooxy group (also known as an alkoxyamine) consists of a primary terminal amine adjacent to oxygen (i.e., —ONH2). This group reacts selectively and efficiently with aldehydes to form an aldoxime linkage and with ketones to form a ketoxime linkage. An oxime bond is more stable than a hydrazone bond and does not need to be reduced to a secondary amine to stabilize it. Aldehydes and ketones occur relatively rarely in nature; however, using sodium periodate, aldehydes can be formed in glycoproteins whose carbohydrate coats contain reducing sugars. Moreover, scientific literature examples demonstrate the introduction of ketones into bacterial cell walls through liposomal fusion.

Specifications

Unit Size 50 mg, 100 mg, 1000 mg
Molecular Weight 823.43; single compound
Chemical formula C₃₄H₆₇CIN₄O₁₄S
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 38 atoms and 44.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₁₁-oxyamine. HCl appeared first on VectorLabs.

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Description

Biotin-dPEG®11-oxyamine·HCl, product number QBD-11100, consists of a medium-length (38 atoms, 44.9 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized at each end with biotin and an oxyamine group (as the HCl salt), respctively. This PEG biotin compound reacts with aldehydes and ketones to forms stable oxime bonds. Using this biotinylation reagent permits biotin labeling of the carbohydrate coats of glycoproteins after the introduction of aldehydes and ketones on the carbohydrate coat using enzymatic (for example, galactose oxidase) or mild chemical reaction conditions such as sodium periodate.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or surfaces such as glass or gold. Bioconjugation research and product development and numerous clinical assays use biotinylated molecules. The amphiphilic dPEG® linker of Biotin-dPEG®11-oxyamine·HCl imparts excellent water solubility to biotin, which is usually poorly soluble in water and causes non-specific hydrophobic interactions when conjugated to biomolecules.

Some commercially available biotin-aminooxy products use either no spacer or a hydrophobic alkyl chain as a spacer between biotin and the aminooxy moiety. Such products have poor solubility in water and must be dissolved in a dry organic solvent such as dimethyl sulfoxide (DMSO) immediately before use, which can be problematic under some conditions. If used on proteins, these hydrophobic products may trigger aggregation and precipitation of the biotinylated molecule. Even if aggregation and precipitation do not occur, the hydrophobic spacer can contribute to non-specific binding. Because it is amphiphilic, Biotin-dPEG®11-oxyamine·HCl can be dissolved in water or aqueous buffer and used directly in a conjugation reaction. QBD-11100 will not cause aggregation or precipitation of the biotinylated molecule. Moreover, the hydrophilicity of the dPEG® linker reduces or eliminates non-specific binding.

An aminooxy group (also known as an alkoxyamine) consists of a primary terminal amine adjacent to oxygen (i.e., —ONH2). This group reacts selectively and efficiently with aldehydes to form an aldoxime linkage and with ketones to form a ketoxime linkage. An oxime bond is more stable than a hydrazone bond and does not need to be reduced to a secondary amine to stabilize it. Aldehydes and ketones occur relatively rarely in nature; however, using sodium periodate, aldehydes can be formed in glycoproteins whose carbohydrate coats contain reducing sugars. Moreover, scientific literature examples demonstrate the introduction of ketones into bacterial cell walls through liposomal fusion.

Specifications

Unit Size50 mg, 100 mg, 1000 mg
Molecular Weight823.43; single compound
Chemical formulaC₃₄H₆₇CIN₄O₁₄S
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 38 atoms and 44.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Biotin-dPEG®₁₁-oxyamine. HCl appeared first on VectorLabs.

]]> Biotin-dPEG®₃-oxyamine. HCl https://staging.vectorlabs.com/products/biotin-dpeg3-oxyamine-hcl/ Sat, 24 Feb 2024 02:14:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=37477 m-dPEG®25-amido-dPEG®24-DSPE, product number QBD-11095, modifies the lipid 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) with a long (153 atoms, 179.8 Å), methoxy-terminated, single molecular weight, discrete polyethylene glycol (dPEG®) spacer. The spacer length is equivalent to a monodispersed mPEG49. This product is designed to protect liposomes and micelles from opsonization and elimination by the reticuloendothelial system (RES). The m-dPEG® spacer offers protection from opsonization comparable to the traditional polymer mPEG2000, but the monodispersed m-dPEG® product is a single compound, not a non-uniform (dispersed) polymer. The methyl-capped dPEG® spacer has a neutral charge.

Vector Laboratories' dPEG® products are highly pure single molecular weight compounds with a discrete chain length. Traditional PEG compounds used to coat liposomes and micelles consist of an intractable mixture of different chain lengths and molecular weights. The non-uniform polymeric mixture leads to irreproducible purity profiles, unlike dPEG® compounds.

Specifications

Unit Size 25 mg, 1000mg
Molecular Weight 471.01; single compound
Chemical formula C₁₈H₃₅CIN₄O₆S
CAS N/A
Purity > 98%
Spacers dPEG® Spacer is 153 atoms and 179.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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Description

Biotin-dPEG®3-oxyamine·HCl, product number QBD-11100, consists of a short (14 atoms, 16.6 Å) single molecular weight, discrete PEG (dPEG®) spacer functionalized at each end with biotin and an oxyamine group (as the HCl salt), respctively. This PEG biotin compound reacts with aldehydes and ketones to forms stable oxime bonds. Using this biotinylation reagent permits biotin labeling of the carbohydrate coats of glycoproteins after the introduction of aldehydes and ketones on the carbohydrate coat using enzymatic (for example, galactose oxidase) or mild chemical reaction conditions such as sodium periodate.

Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or surfaces such as glass or gold. Bioconjugation research and product development and numerous clinical assays use biotinylated molecules. The amphiphilic dPEG® linker of Biotin-dPEG®3-oxyamine·HCl imparts excellent water solubility to biotin, which is usually poorly soluble in water and causes non-specific hydrophobic interactions when conjugated to biomolecules.

Some commercially available biotin-aminooxy products use either no spacer or a hydrophobic alkyl chain as a spacer between biotin and the aminooxy moiety. Such products have poor solubility in water and must be dissolved in a dry organic solvent such as dimethyl sulfoxide (DMSO) immediately before use, which can be problematic under some conditions. If used on proteins, these hydrophobic products may trigger aggregation and precipitation of the biotinylated molecule. Even if aggregation and precipitation do not occur, the hydrophobic spacer can contribute to non-specific binding. Because it is amphiphilic, Biotin-dPEG®3-oxyamine·HCl can be dissolved in water or aqueous buffer and used directly in a conjugation reaction. QBD-11100 will not cause aggregation or precipitation of the biotinylated molecule. Moreover, the hydrophilicity of the dPEG® linker reduces or eliminates non-specific binding.

An aminooxy group (also known as an alkoxyamine) consists of a primary terminal amine adjacent to oxygen (i.e., —ONH2). This group reacts selectively and efficiently with aldehydes to form an aldoxime linkage and with ketones to form a ketoxime linkage. An oxime bond is more stable than a hydrazone bond and does not need to be reduced to a secondary amine to stabilize it. Aldehydes and ketones occur relatively rarely in nature; however, using sodium periodate, aldehydes can be formed in glycoproteins whose carbohydrate coats contain reducing sugars. Moreover, scientific literature examples demonstrate the introduction of ketones into bacterial cell walls through liposomal fusion.

Specifications

Unit Size25 mg, 1000mg
Molecular Weight471.01; single compound
Chemical formulaC₁₈H₃₅CIN₄O₆S
CASN/A
Purity> 98%
SpacersdPEG® Spacer is 153 atoms and 179.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

 

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 276-335 for general description and use of heterobifunctional crosslinkers, and the specific sample protocol for SPDP and LC-SPDP on pp. 286-288. See Greg’s extensive index on pp. 1192-1193 for references to a number and range of applications and their respective protocols.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Reduced Sialylation Impacts Ventricular Repolarization by Modulating Specific K+ Channel Isoforms Distinctly. Andrew R. Ednie and Eric S. Bennett. Journal of Biological Chemistry. 2014. December 18, 2014. DOI: 10.1074/jbc.M114.605139.

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]]> Biotin-dPEG®₂₃-Lipoamide https://staging.vectorlabs.com/products/biotin-dpeg23-lipoamide/ Sat, 24 Feb 2024 02:13:28 +0000 https://staging.vectorlabs.com/?post_type=product&p=37026 Biotin-dPEG®23-lipoamide, product number 10828, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a long (73 atoms, 87.6 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1487.92; single compound
Chemical formula C₆₆H₁₂₆N₄O₂₆S₃
CAS N/A
Purity > 96%
Spacers dPEG® Spacer is 73 atoms and 87.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene Chloride, DMSO, DMAC, Acetonitrile or DMF.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₂₃-Lipoamide appeared first on VectorLabs.

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Description

Biotin-dPEG®23-lipoamide, product number QBD-10828, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a long (73 atoms, 87.6 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1487.92; single compound
Chemical formulaC₆₆H₁₂₆N₄O₂₆S₃
CASN/A
Purity> 96%
SpacersdPEG® Spacer is 73 atoms and 87.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene Chloride, DMSO, DMAC, Acetonitrile or DMF.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), pp. 188-190, 458-497, 924-935.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Biotin-dPEG®₂₃-Lipoamide appeared first on VectorLabs.

]]> Biotin-dPEG®₇-NH₂ https://staging.vectorlabs.com/products/biotin-dpeg7-nh2/ Sat, 24 Feb 2024 02:13:27 +0000 https://staging.vectorlabs.com/?post_type=product&p=37018 Biotin-dPEG®7-NH2 (Biotin-dPEG®7-amine), product number QBD-10826, is a short (25 atoms, 29.8 Å), hydrophilic, water-soluble, discrete PEG biotinylation product that reacts with carboxylic acids and their activated esters [N-hydroxysuccinimidyl (NHS), 2,3,5,6-tetrafluorophenyl (TFP)] to form stable amide bonds. Additionally, Biotin-dPEG®7-NH2 forms amide bonds directly with carboxylic acids using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Furthermore, the product can react with carbonyl groups (aldehydes or ketones) to form a labile Schiff base, which can be reduced to a secondary amine using sodium cyanoborohydride for additional conjugate stability.

Amphiphilic biotin-dPEG®7-NH2 dissolves equally well in both aqueous buffer and organic solvent. It is not necessary to dissolve this product in an organic solvent before using it in aqueous reaction media. Furthermore, biotinylation of biomacromolecules with QBD-10826 will not cause aggregation and precipitation. Eliminating aggregation should consequently improve the signal-to-noise ratio of applications developed with this product. Aldehydes are uncommon in biomacromolecules. However, the oxidation of reducing sugars in glycosylated proteins (for example, using sodium periodate) forms aldehydes on the carbohydrate coats of glycoproteins. Reacting Biotin-dPEG®7-amine with aldehydes will produce labile imines or Schiff bases that can be reduced under mild conditions with sodium cyanoborohydride to form more stable secondary amines.

Any application that can take advantage of the strong biotin-avidin/ streptavidin affinity can use Biotin-dPEG®7-NH2. The terminal amine of QBD-10826 offers the opportunity to biotinylate (1) the carbohydrate coat of glycoproteins and (2) surfaces consisting of carboxylic acids or their active esters. Thus, Biotin-dPEG®7-NH2 expands the range of molecules and nanoparticles for which dPEG®-biotin is useful.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 594.76; single compound
Chemical formula C₂₆H₅₀N₄O₉S
CAS 1334172-76-7
Purity > 98%
Spacers dPEG® Spacer is 25 atoms and 29.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₇-NH₂ appeared first on VectorLabs.

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Description

Biotin-dPEG®7-NH2 (Biotin-dPEG®7-amine), product number QBD-10826, is a short (25 atoms, 29.8 Å), hydrophilic, water-soluble, discrete PEG biotinylation product that reacts with carboxylic acids and their activated esters [N-hydroxysuccinimidyl (NHS), 2,3,5,6-tetrafluorophenyl (TFP)] to form stable amide bonds. Additionally, Biotin-dPEG®7-NH2 forms amide bonds directly with carboxylic acids using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Furthermore, the product can react with carbonyl groups (aldehydes or ketones) to form a labile Schiff base, which can be reduced to a secondary amine using sodium cyanoborohydride for additional conjugate stability.

Amphiphilic biotin-dPEG®7-NH2 dissolves equally well in both aqueous buffer and organic solvent. It is not necessary to dissolve this product in an organic solvent before using it in aqueous reaction media. Furthermore, biotinylation of biomacromolecules with QBD-10826 will not cause aggregation and precipitation. Eliminating aggregation should consequently improve the signal-to-noise ratio of applications developed with this product. Aldehydes are uncommon in biomacromolecules. However, the oxidation of reducing sugars in glycosylated proteins (for example, using sodium periodate) forms aldehydes on the carbohydrate coats of glycoproteins. Reacting Biotin-dPEG®7-amine with aldehydes will produce labile imines or Schiff bases that can be reduced under mild conditions with sodium cyanoborohydride to form more stable secondary amines.

Any application that can take advantage of the strong biotin-avidin/ streptavidin affinity can use Biotin-dPEG®7-NH2. The terminal amine of QBD-10826 offers the opportunity to biotinylate (1) the carbohydrate coat of glycoproteins and (2) surfaces consisting of carboxylic acids or their active esters. Thus, Biotin-dPEG®7-NH2 expands the range of molecules and nanoparticles for which dPEG®-biotin is useful.

Specifications

Unit Size100mg, 1000mg
Molecular Weight594.76; single compound
Chemical formulaC₂₆H₅₀N₄O₉S
CAS1334172-76-7
Purity> 98%
SpacersdPEG® Spacer is 25 atoms and 29.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 529-530 for a general discussion in Greg’s book and on biotinylation as well, as well as pp. 737-738 for a protocol for a compound with just two oxygen. However, other than enhanced solubility and binding distances, the protocol is adaptable to our PN 10193.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Protein Microarray Immobilization via Epoxide Ring-Opening by Thiol, Amine, and Azide. Seyed Mohammad Mahdi Dadfar, Sylwia Sekula-Neuner, Vanessa Trouillet, Michael Hirtz. Advanced Materials Interfaces. 2021. Volume 8, Issue 10. March 18, 2021. https://doi.org/10.1002/admi.202002117

The post Biotin-dPEG®₇-NH₂ appeared first on VectorLabs.

]]> Biotin-dPEG®₇-azide https://staging.vectorlabs.com/products/biotin-dpeg7-azide/ Sat, 24 Feb 2024 02:13:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=37010 Biotin-dPEG®7-azide, product number QBD-10825, is a water-soluble biotinylation product functionalized with azide for click chemistry use on a short (27 atoms, 30.7 Å) single molecular weight, discrete PEG (dPEG®) chain. This product enables the biotinylation of molecules alkyne-containing molecules and surfaces using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Moreover, the hydrophilic discrete PEG spacer makes biotin highly water soluble, reducing or eliminating aggregation and precipitation of biotin-labeled biomolecules triggered by hydrophobic interactions. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®7-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 620.32; single compound
Chemical formula C₂₆H₄₈N₆O₉S
CAS 1334172-75-6
Purity > 98%
Spacers dPEG® Spacer is 27 atoms and 30.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₇-azide appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®7-azide, product number QBD-10825, is a water-soluble biotinylation product functionalized with azide for click chemistry use on a short (27 atoms, 30.7 Å) single molecular weight, discrete PEG (dPEG®) chain. This product enables the biotinylation of molecules alkyne-containing molecules and surfaces using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Moreover, the hydrophilic discrete PEG spacer makes biotin highly water soluble, reducing or eliminating aggregation and precipitation of biotin-labeled biomolecules triggered by hydrophobic interactions. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®7-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Specifications

Unit Size100mg, 1000mg
Molecular Weight620.32; single compound
Chemical formulaC₂₆H₄₈N₆O₉S
CAS1334172-75-6
Purity> 98%
SpacersdPEG® Spacer is 27 atoms and 30.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.

Palmitoylation of superoxide dismutase 1(SOD1) is increased for familial ALS-linked SOD1 mutants. Sarah E. Antinone, Ghanashyam D. Ghadge, TuKiet T. Lam, Lijun Wang, Raymond P. Roos and William N. Green. J. Biol. Chem. 2013, 288 (49) pp 1-25. June 12, 2013. DOI: 10.1074/jbc.M113.487231.

Herpes Simplex Virus 2 Infection Impacts Stress Granule Accumulation. Renée L. Finnen, Kyle R. Pangka, and Bruce W. Banfield. J. Virol. 2012, 86 (15) pp 8119–8130. August 1, 2012. doi:10.1128/JVI.00313-12.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.

Polymer Therapeutics with a Coiled Coil Motif Targeted against Murine BCL1 Leukemia. Robert Pola, Richard Laga, Karel Ulbrich, Irena Sieglová, Vlastimil Král, Milan Fábry, Martina Kabešová, Marek Kovář, and Michal Pechar. Biomacromolecules. 2013, 14 (3) pp 881–889. February 1, 2013. DOI: 10.1021/bm3019592.

Sequential Nucleophilic Substitutions Permit Orthogonal Click Functionalization of Multicomponent PEG Brushes. Jin Sha, Ethan S. Lippmann, Jason McNulty, Yulu Ma, and Randolph S. Ashton. Biomacromolecules. 2013, 14 (9) pp 3294–3303. August 13, 2013. DOI: 10.1021/bm400900r.

Detection of O -GlcNAc Modi fi cations on Cardiac Myo fi lament Proteins. Genaro A. Ramirez-Correa , Isabel Martinez Ferrando , Gerald Hart and Anne Murphy. Methods in Molecular Biology. 2013, 1005 (13) pp 157-168. January 1, 2013. DOI: 10.1007/978-1-62703-386-2_13.

The post Biotin-dPEG®₇-azide appeared first on VectorLabs.

]]> Biotin-dPEG®₁₁-Lipoamide https://staging.vectorlabs.com/products/biotin-dpeg11-lipoamide/ Sat, 24 Feb 2024 02:13:24 +0000 https://staging.vectorlabs.com/?post_type=product&p=37002 Biotin-dPEG®11-lipoamide, product number 10822, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a medium-length (43 atoms, 52.9 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 959.28; single compound
Chemical formula C₄₂H₇₈N₄O₁₄S₃
CAS 960069-81-2
Purity > 96%
Spacers dPEG® Spacer is 43 atoms and 52.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₁₁-Lipoamide appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®11-lipoamide, product number QBD-10822, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a medium-length (43 atoms, 52.9 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size100mg, 1000mg
Molecular Weight959.28; single compound
Chemical formulaC₄₂H₇₈N₄O₁₄S₃
CAS960069-81-2
Purity> 96%
SpacersdPEG® Spacer is 43 atoms and 52.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), pp. 188-190, 458-497, 924-935.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Biotin-dPEG®₁₁-Lipoamide appeared first on VectorLabs.

]]> Biotin-dPEG®₃-Lipoamide https://staging.vectorlabs.com/products/biotin-dpeg3-lipoamide/ Sat, 24 Feb 2024 02:13:23 +0000 https://staging.vectorlabs.com/?post_type=product&p=36994 Biotin-dPEG®3-lipoamide, product number 10820, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a short (22 atoms, 27.7 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 634.91; single compound
Chemical formula C₂₈H₅₀N₄O₆S₃
CAS 1334172-74-5
Purity > 96%
Spacers dPEG® Spacer is 22 atoms and 27.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMAC, DMSO, acetonitrile, poorly soluble in methylene chloride. Insoluble in water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₃-Lipoamide appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®3-lipoamide, product number QBD-10820, is a water-soluble PEGylation reagent designed to facilitate the biotinylation of a gold, silver, or other metal surface through a short (22 atoms, 27.7 Å) lipoic acid-functionalized single molecular weight, discrete PEG (dPEG®) spacer. The lipoic acid moiety forms dative bonds with gold, silver, and other metals, while the flexible, hydrophilic dPEG® spacer increases the hydrodynamic volume of the conjugate surface. This reduces or eliminates opsonization of the conjugate, contributing to a longer serum circulation time in vivo. Moreover, the water-soluble dPEG® linker improves the hydrophilicity of the biotin label, which is poorly soluble in water, reducing aggregation, precipitation, and non-specific interactions due to hydrophobicity. Potential applications for this product include pull-down assays, affinity chromatography, and biotin-based antibody pretargeting for radioimmunotherapy.

Specifications

Unit Size100mg, 1000mg
Molecular Weight634.91; single compound
Chemical formulaC₂₈H₅₀N₄O₆S₃
CAS1334172-74-5
Purity> 96%
SpacersdPEG® Spacer is 22 atoms and 27.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)DMAC, DMSO, acetonitrile, poorly soluble in methylene chloride. Insoluble in water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), pp. 188-190, 458-497, 924-935.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Biotin-dPEG®₃-Lipoamide appeared first on VectorLabs.

]]> Biotin-dPEG®₂₃-azide https://staging.vectorlabs.com/products/biotin-dpeg23-azide/ Sat, 24 Feb 2024 02:13:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=36844 Biotin-dPEG®23-azide, product number QBD-10787, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a long (73 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®23-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®23-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Vector Laboratories to create new compounds tailored to the customer’s specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).

As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1325.60; single compound
Chemical formula C₅₈H₁₁₂N₆O₂₅S
CAS 956494-20-5
Purity > 98%
Spacers dPEG® Spacer is 73 atoms and 87.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₂₃-azide appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®23-azide, product number QBD-10787, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a long (73 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®23-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®23-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Vector Laboratories to create new compounds tailored to the customer’s specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).

As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1325.60; single compound
Chemical formulaC₅₈H₁₁₂N₆O₂₅S
CAS956494-20-5
Purity> 98%
SpacersdPEG® Spacer is 73 atoms and 87.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w

The post Biotin-dPEG®₂₃-azide appeared first on VectorLabs.

]]> Biotin-dPEG®₂₃-NH₂ https://staging.vectorlabs.com/products/biotin-dpeg23-nh2/ Sat, 24 Feb 2024 02:13:04 +0000 https://staging.vectorlabs.com/?post_type=product&p=36836 Biotin-dPEG®23-NH2 (Biotin-dPEG®23-amine), product number QBD-10786, is a carboxylate-reactive biotinylation product on a long (71 atoms), single molecular weight, discrete PEG (dPEG®) spacer. Biotin-dPEG®23-NH2 reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under slightly basic conditions. Moreover, it can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Additionally, the product reacts with aldehydes or ketones to form a labile Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary.

Amphiphilic Biotin-dPEG®23-NH2 dissolves equally well in both aqueous media and organic solvents. Therefore, it is unnecessary to dissolve this product in an organic solvent before using it in an aqueous reaction. Furthermore, biotinylation of biomacromolecules with Biotin-dPEG®23-NH2 will not cause aggregation and precipitation. Eliminating aggregation and precipitation improves the signal-to-noise ratio of applications developed with this product. Reactions with carboxylic acids or active esters of carboxylic acids yield stable amide bonds. Aldehydes are not commonly found in biomacromolecules but can be formed by oxidizing reducing sugars in glycosylated proteins. Reactions with aldehydes create labile imines (Schiff bases) that can be reduced under mild conditions with sodium cyanoborohydride to form more stable secondary amines.

Biotin-dPEG®23-NH2 works in any application that can take advantage of the exceptionally strong biotin-avidin/streptavidin affinity. The terminal amine of QBD-10786 offers the opportunity (1) to biotinylate the carbohydrate coat of glycoproteins by oxidizing reducing sugars to aldehydes and (2) to biotinylate surfaces consisting of carboxylic acids or their active esters. Thus, this product expands the range of molecules and nanoparticles in which dPEG®-biotin can be employed.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1299.60; single compound
Chemical formula C₅₈H₁₁₄N₄O₂₅S
CAS 604786-74-5
Purity > 95%
Spacers dPEG® Spacer is 71 atoms and 87.0 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₂₃-NH₂ appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®23-NH2 (Biotin-dPEG®23-amine), product number QBD-10786, is a carboxylate-reactive biotinylation product on a long (71 atoms), single molecular weight, discrete PEG (dPEG®) spacer. Biotin-dPEG®23-NH2 reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under slightly basic conditions. Moreover, it can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Additionally, the product reacts with aldehydes or ketones to form a labile Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary.
Amphiphilic Biotin-dPEG®23-NH2 dissolves equally well in both aqueous media and organic solvents. Therefore, it is unnecessary to dissolve this product in an organic solvent before using it in an aqueous reaction. Furthermore, biotinylation of biomacromolecules with Biotin-dPEG®23-NH2 will not cause aggregation and precipitation. Eliminating aggregation and precipitation improves the signal-to-noise ratio of applications developed with this product. Reactions with carboxylic acids or active esters of carboxylic acids yield stable amide bonds. Aldehydes are not commonly found in biomacromolecules but can be formed by oxidizing reducing sugars in glycosylated proteins. Reactions with aldehydes create labile imines (Schiff bases) that can be reduced under mild conditions with sodium cyanoborohydride to form more stable secondary amines.

Biotin-dPEG®23-NH2 works in any application that can take advantage of the exceptionally strong biotin-avidin/streptavidin affinity. The terminal amine of QBD-10786 offers the opportunity (1) to biotinylate the carbohydrate coat of glycoproteins by oxidizing reducing sugars to aldehydes and (2) to biotinylate surfaces consisting of carboxylic acids or their active esters. Thus, this product expands the range of molecules and nanoparticles in which dPEG®-biotin can be employed.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1299.60; single compound
Chemical formulaC₅₈H₁₁₄N₄O₂₅S
CAS604786-74-5
Purity> 95%
SpacersdPEG® Spacer is 71 atoms and 87.0 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0); See pp. 529-530 for a general discussion in Greg’s book and on biotinylation as well, as well as pp. 737-738 for a protocol for a compound with just two oxygen. However, other than enhanced solubility and binding distances, the protocol is adaptable to our PN 10193.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Blood Plasma-Derived Anti-Glycan Antibodies to Sialylated and Sulfated Glycans Identify Ovarian Cancer Patients. Tatiana Pochechueva, Alexander Chinarev, Andreas Schoetzau, Andre Fedier, Nicolai V. Bovin, Neville F. Hacker, Francis Jacob, Viola Heinzelmann-Schwarz. Plos One. 2016, 11 (10) e0164230. October 20, 2016. DOI: 10.1371/journal.pone.0164230.

Poly(catecholamine) coating and biofunctionalization of CsPbBr3 microcrystals. Sangyeon Cho and Seok Hyun Yun. ChemRxiv. 2021. License: CC BY-NC-ND 4.0. DOI: 10.26434/chemrxiv.14049710.v2

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