Bis Biotin – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:17:33 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Bis Biotin – VectorLabs https://staging.vectorlabs.com 32 32 Bis-dPEG®₃-biotin https://staging.vectorlabs.com/products/bis-dpeg3-biotin/ Sat, 24 Feb 2024 02:10:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=36002 Bis-dPEG®3-biotin, product number QBD-10325, is a novel biotin-labeling product composed of a dPEG®3 spacer with biotin attached to each end of the spacer, Bis-dPEG®3-biotin offers customers unique opportunities for exploiting the biotin-avidin/streptavidin affinity since it can bind to two different avidin/streptavidin binding sites, both intramolecularly and intermolecularly. Indeed, this product was developed specifically to block endogenous biotin found in all cells and tissues so that it did not interfere with targeted biotin-streptavidin interactions in therapeutic and diagnostic applications where endogenous biotin was known to cause problems.

The binding behavior of Bis-dPEG®3-biotin with streptavidin opens up possible uses for the product in the self-assembly of supramolecular constructs and signal amplification. In the first example, if two parts of a potential supramolecular construct both contain avidin or streptavidin, addition of QBD-10325 under empirically determined optimized conditions could trigger self-assembly of the construct as the bis-dPEG®3-biotin binds to the separate avidin or streptavidin molecules in the two halves of the construct. In the second example, multiple streptavidin molecules conjugated to horseradish peroxidase (HRP) could be bound together with a streptavidin-biotinylated protein complex (for example, a biotinylated antibody), thus amplifying the signal from HRP.

Specifications

Unit Size 50 mg, 1000 mg
Molecular Weight 672.90; single compound
Chemical formula C₃₀H₅₂N₆O₇S₂
CAS 194920-54-2
Purity > 98%
Spacers dPEG® Spacer is 15 atoms and 18.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Bis-dPEG®₃-biotin appeared first on VectorLabs.

]]>

Description

Bis-dPEG®3-biotin, product number QBD-10325, is a novel biotin-labeling product composed of a dPEG®3 spacer with biotin attached to each end of the spacer, Bis-dPEG®3-biotin offers customers unique opportunities for exploiting the biotin-avidin/streptavidin affinity since it can bind to two different avidin/streptavidin binding sites, both intramolecularly and intermolecularly. Indeed, this product was developed specifically to block endogenous biotin found in all cells and tissues so that it did not interfere with targeted biotin-streptavidin interactions in therapeutic and diagnostic applications where endogenous biotin was known to cause problems.

The binding behavior of Bis-dPEG®3-biotin with streptavidin opens up possible uses for the product in the self-assembly of supramolecular constructs and signal amplification. In the first example, if two parts of a potential supramolecular construct both contain avidin or streptavidin, addition of QBD-10325 under empirically determined optimized conditions could trigger self-assembly of the construct as the bis-dPEG®3-biotin binds to the separate avidin or streptavidin molecules in the two halves of the construct. In the second example, multiple streptavidin molecules conjugated to horseradish peroxidase (HRP) could be bound together with a streptavidin-biotinylated protein complex (for example, a biotinylated antibody), thus amplifying the signal from HRP.

Specifications

Unit Size50 mg, 1000 mg
Molecular Weight672.90; single compound
Chemical formulaC₃₀H₅₂N₆O₇S₂
CAS194920-54-2
Purity> 98%
SpacersdPEG® Spacer is 15 atoms and 18.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

The post Bis-dPEG®₃-biotin appeared first on VectorLabs.

]]>