Biotin (water soluble) – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:02:34 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin (water soluble) – VectorLabs https://staging.vectorlabs.com 32 32 Sulfo ChromaLINK® Biotin (Water Soluble) https://staging.vectorlabs.com/products/chromalink-biotin-antibody-labeling-h20sol/ Wed, 03 May 2023 04:13:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=14796 Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple, non-destructive UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. This biotinylation reagent is water soluble, which means no DMF or other organic solvent is required to dissolve the compound prior to labeling.

Specifications

Label/Modifier Type biotin
Reactivity Streptavidin
Recommended Storage Desiccated: -15° to -25°C
Applications Antibody Labeling, Aptamers
Other Name(s) SoluLINK Bioconjugation

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Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple, non-destructive UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. This biotinylation reagent is water soluble, which means no DMF or other organic solvent is required to dissolve the compound prior to labeling.

Specifications

Label/Modifier Typebiotin
ReactivityStreptavidin
Recommended StorageDesiccated: -15° to -25°C
ApplicationsAntibody Labeling, Aptamers
Other Name(s)SoluLINK Bioconjugation

Documents

Citations

Technical Information

Introduction to ChromaLINK Labeling Technology

ChromaLINK Biotin incorporates UV-traceable biotin onto proteins containing lysine residues (amine groups) via a water-soluble-succinimidyl activated ester. ChromaLINK Biotin has been engineered to include many novel features. As illustrated in Figure 1, the molecule’s structure contains a bis-aryl hydrazone chromophore (a), linked by a PEG3 linker arm (b), to biotin (c). This reagent permits direct spectroscopic quantification of incorporated biotin. The extended PEG3 linker preserves biotin/streptavidin affinity and maintains protein solubility after modification while the succinimidyl ester functional group (d), efficiently modifies lysines in aqueous buffers.

B 1007 F1

Figure 1. Molecular structure of ChromaLINK Biotin (water soluble)

Labeling of proteins with ChromaLINK Biotin eliminates the need to carry out cumbersome and time-consuming HABA assays often employed to quantify biotin incorporation. Instead, biotin incorporation is quantified by means of a simple spectrophotometric measurement at two wavelengths (A280 / A354). Typical labeling results are illustrated in Figure 2 by spectral overlay scans of four samples. As illustrated, bovine IgG (100 ul @ 5 mg/ml) was labeled at 0, 5,10, and 15 mole equivalents using ChromaLINK Biotin. Spectral analysis illustrates how easy it is to visualize, confirm, and quantify biotin incorporation.

B 1007 F2 jpg

Figure 2. Superimposed spectra of bovine IgG biotinylated using ChromaLINK Biotin. Various biotin-to-protein mole equivalents (5X, 10X and 15X) were used. Note the traceable UV-signature @ 354nm indicating incorporation of biotin. All spectra were scanned on a Molecular Dynamics SpectraMax PlusTM UV-VIS plate reader (220-420 nm).

Legal Information

SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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