Biotin Maleimide – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:04 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin Maleimide – VectorLabs https://staging.vectorlabs.com 32 32 Biotin-dPEG®₂₃-MAL https://staging.vectorlabs.com/products/biotin-dpeg23-mal/ Sat, 24 Feb 2024 02:13:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=36821 Biotin-dPEG®23-MAL, product number 10785, is a thiol-reactive biotinylation product built on a long (77 atoms), single molecular weight, discrete PEG (dPEG®) spacer. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product is highly hydrophilic and increases the conjugate's water solubility.

Specifications

Unit Size 25 mg, 100 mg, 1000 mg
Molecular Weight 1450.72; single compound
Chemical formula C₆₅H₁₁₉N₅O₂₈S
CAS 1334172-60-9
Purity > 95%
Spacers dPEG® Spacer is 77 atoms and 94.1 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

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]]>

Description

Biotin-dPEG®23-MAL, product number QBD-10785, is a thiol-reactive biotinylation product built on a long (77 atoms), single molecular weight, discrete PEG (dPEG®) spacer. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product is highly hydrophilic and increases the conjugate’s water solubility.

Specifications

Unit Size25 mg, 100 mg, 1000 mg
Molecular Weight1450.72; single compound
Chemical formulaC₆₅H₁₁₉N₅O₂₈S
CAS1334172-60-9
Purity> 95%
SpacersdPEG® Spacer is 77 atoms and 94.1 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Integrin-Generated Forces Lead to Streptavidin-Biotin Unbinding in Cellular Adhesions. Carol Jurchenko, Yuan Chang, Yoshie Narui, Yun Zhang, Khalid S. Salaita. Biophysical Journal. 2014, 106 (7) pp 1436-1446. 4/1/2017. DOI: 10.1016/j.bpj.2014.01.049.

The post Biotin-dPEG®₂₃-MAL appeared first on VectorLabs.

]]> Biotin-dPEG®₃-MAL https://staging.vectorlabs.com/products/biotin-dpeg3-mal/ Sat, 24 Feb 2024 02:07:03 +0000 https://staging.vectorlabs.com/?post_type=product&p=35335 Biotin-dPEG®3-MAL, product number 10201, consists of biotin on one end of a dPEG®3 linker with a maleimidopropyl moiety (maleimide ring connected to propionic acid) on the other end. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solublity to conjugates formed with it. The maleimide group reacts with free thiols to form stable thioether linkages.

Description

"Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other. For QBD-10201, the linker is a dPEG®3 product. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solubility to conjugates formed with it. The maleimide moiety is a popular reactive group for conjugating to free thiol groups in biomolecules and other sulfhydryl compounds. Maleimide groups can be conjugated to free thiol groups in the pH range 6.5 – 7.5, forming a stable thioether linkage.

This product has proven helpful in various applications that utilize the high streptavidin-biotin binding affinity. Such applications include
FRET-based assays
probes of protein topology
protein labeling
the creation of biotinylated, dPEG®-modified nucleosides
different types of high-throughput screening procedures
the construction of biomimetic gold-and-silica nanoparticles, and
the construction of self-assembling quantum dots.

To use Biotin-dPEG®3-MAL, dissolve the product in a dry solvent and add it to an aqueous compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), care should be taken to use a minimal amount of organic solvent so as not to denature the biomolecule or cause salt precipitation."

Specifications

Unit Size 50 mg
Molecular Weight 597.73; single compound
Chemical formula C₂₇H₄₃N₅O₈S
CAS 525573-22-2
Purity > 98%
Spacers dPEG® Spacer is 21 atoms and 24.9 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₃-MAL appeared first on VectorLabs.

]]>

Biotin-dPEG®3-MAL, product number 10201, consists of biotin on one end of a dPEG®3 linker with a maleimidopropyl moiety (maleimide ring connected to propionic acid) on the other end. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solublity to conjugates formed with it. The maleimide group reacts with free thiols to form stable thioether linkages.

Description

Biotin-dPEG®3-MAL, product number 10201, is one of three products containing biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other. For QBD-10201, the linker is a dPEG®3 product. The flexible, short-chain, discrete PEG (dPEG®) linker imparts water solubility to conjugates formed with it. The maleimide moiety is a popular reactive group for conjugating to free thiol groups in biomolecules and other sulfhydryl compounds. Maleimide groups can be conjugated to free thiol groups in the pH range 6.5 – 7.5, forming a stable thioether linkage.

This product has proven helpful in various applications that utilize the high streptavidin-biotin binding affinity. Such applications include
FRET-based assays
probes of protein topology
protein labeling
the creation of biotinylated, dPEG®-modified nucleosides
different types of high-throughput screening procedures
the construction of biomimetic gold-and-silica nanoparticles, and
the construction of self-assembling quantum dots.

To use Biotin-dPEG®3-MAL, dissolve the product in a dry solvent and add it to an aqueous compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent for this purpose. For biomolecules (e.g., antibodies), care should be taken to use a minimal amount of organic solvent so as not to denature the biomolecule or cause salt precipitation.

Specifications

Unit Size50 mg
Molecular Weight597.73; single compound
Chemical formulaC₂₇H₄₃N₅O₈S
CAS525573-22-2
Purity> 98%
SpacersdPEG® Spacer is 21 atoms and 24.9 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Solid Phase 4’-Phosphopantetheinylation: Fungal Thiolation Domains are Targets for Chemoenzymatic Modification, Deirdre Stack, Aisling Frizzell, Karen Tomkins, and Sean Doyle. Bioconjugate Chem. 2009, 20 (8), pp 1514–1522. July 23, 2009. DOI: 10.1021/bc900071j.

Manipulation of Carrier Proteins in Antibiotic Biosynthesis, James J. La Clair, Timothy L. Foley, Tracy R. Schegg, Conor M. Regan, and Michael D. Burkart. Chemistry & Biology. 2004. 11 (2), pp 195-201. February 2004. DOI: 10.1016/j.chembiol.2004.02.010.

Biomimetic approach to the formation of gold nanoparticle/silica cor/shell structures and subsequent bioconjugation, Sung Min Kang, Kyung-Bok Lee, Dong Jin Kim and Insung S Choi. Nanotechnology. 2006, (17), pp. 4719–4725. September 1, 2006. DOI: 10.1088/0957.

Probing the Topological Tolerance of Multimeric Protein Interactions: Evaluation of an Estrogen/Synthetic Ligand for FK506 Binding Protein Conjugate. Terry W. Moore Jillian R. Gunther, and John A. Katzenellenbogen. Bioconjugate Chem. 2010, 21 (10), pp 1880–1889. October 4, 2010. DOI: 10.1021/bc100266v.

Exploration of Dimensions of Estrogen Potency Parsing Ligand Binding and Coactivator Binding Affinities. M. Jeyakumar, Kathryn E. Carlson, Jillian R. Gunther, and John A. Katzenellenbogen. Journal of Biological Chemistry. 2011 (286), pp. 12971-12982. February 14, 2011. DOI: 10.1074/jbc.M110.205112.

Monitoring a Coordinated Exchange Process in a Four-Component Biological Interaction System: Development of a Time-Resolved Terbium-Based One Donor/Three-Acceptor Multi-Color FRET System. Sung Hoon Kim, Jillian R. Gunther, and John A. Katzenellenbogen. JACS. 2010 132 (13) pp 4685-4692. April 7, 2011. DOI:10.1021/ja100248q.

Diarylpropionitrile (DPN) Enantiomers: Synthesis and Evaluation of Estrogen Receptor β-Selective Ligands. Vincent M. Carroll, M. Jeyakumar, Kathryn E. Carlson, and John A. Katzenellenbogen. J. Med. Chem. 2012, 55 (1) pp 528–537. November 28, 2011. DOI: 10.1021/jm201436k.


Solid Phase 4′-Phosphopantetheinylation: Fungal Thiolation Domains are Targets for Chemoenzymatic Modification.Deirdre Stack, Aisling Frizzell, Karen Tomkins, and Sean Doyle. Bioconjugate Chem. 2009, 20 (8) pp 1514–1522. July 23, 2009. DOI: 10.1021/bc900071j.

Attachment of hydrogel microstructures and proteins to glass via thiol-terminated silanes. Jeong Hyun Seo, Dong-Sik Shin, Priam Mukundan, Alexander Revzin. Colloids and Surfaces B: Biointerfaces. 2012, 98, pp 1–6. October 1, 2012. doi.org/10.1016/j.colsurfb.2012.03.025.

A Set of Time-Resolved Fluorescence Resonance Energy Transfer Assays for the Discovery of Inhibitors of Estrogen Receptor-Coactivator Binding. Jillian R. Gunther, Yuhong Du, Eric Rhoden, Iestyn Lewis, Brian Revennaugh, Terry W. Moore, Sung Hoon Kim, Raymond Dingledine, Haian Fu and John A. Katzenellenbogen. Journal of Biomolecular Screening. 2009, 14 (2) pp # 181-195. February 4, 2009. DOI: 10.1177/1087057108329349.

Structural characterization and functionalization of engineered spider silk films. Kristina Spieß, Stefanie Wohlrab and Thomas Scheibel. Soft Matter. 2010, 6 (17) pp 4168-4174. June 16, 2010. DOI: 10.1039/b927267d.

Enzyme-independent, orientation-selective conjugation of whole human complement C3 to protein surfaces. Daniel A. Mitchell, Rebecca Ilyas, Alister W. Dodds, Robert B. Sim. Journal of Immunological Methods. 2008, 337 (1) pp 49-54. June 9, 2008. DOI: 10.1016/j.jim.2008.05.011.

Colorimetric multiplexed immunoassay using specific aggregation of antigenic peptide-modified luminous nanoparticles. Toshihiro Ihara, Yasunori Mori, Takaaki Imamura, Motoko Mukae, Shojiro Tanaka, Akinori Jyo. Science Direct. 2006, 578 (1) pp 11-18. May 4, 2006. DOI: 10.1016/j.aca.2006.04.066.

An Anti-Insulin-like Growth Factor I Receptor AntibodyIs a Potent Inhibitor of Cancer Cell Proliferation. Erin K. Maloney, Jennifer L. McLaughlin, Nancy E. Dagdigian. Cancer Research. 2003, 63 pp 5073-5083. August 15, 2003. DOI:

5’-Sulfhydryl-Modified RNA: Initiator Synthesis, in Vitro Transcription, and Enzymatic Incorporation. Lei Zhang, Lele Sun, Zhiyong Cui, Robert L. Gottlieb and Biliang Zhang. Bioconjugate Chem.2001, 12 (6) pp 939–948. October 16, 2001. DOI: 10.1021/bc015504g.

Sugar-Binding Proteins from Fish: Selection of High Affinity “Lambodies” That Recognize Biomedically Relevant Glycans. Xia Hong, Mark Z. Ma, Jeffrey C. Gildersleeve, Sudipa Chowdhury, Joseph J. Barchi, Jr., Roy A. Mariuzza,∥ Michael B. Murphy, Li Mao and Zeev Pancer. ACS Chem. Biol. 2013, 8 pp 152−160. October 2, 2012. doi.org/10.1021/cb300399s.

High-Throughput Screening of Small Molecules Identifies Hepcidin Antagonists. Eileen Fung, Priscilla Sugianto, Jason Hsu, Robert Damoiseaux, Tomas Ganz, and Elizabeta Nemeth. Mol Pharmacol. 2013, 83 (3) pp 681-690. January 4, 2013. DOI.org/10.1124/mol.112.083428.

Self-Assembled Quantum Dot-Bioconjugates: Characterization and Use for Sensing Proteolytic Activity. Igor L. Medintz1, Thomas Pons, Kim E. Sapsford, Philip E. Dawson and Hedi Mattoussi. Proc. of SPIE. 2008, 6945. April 15, 2008. DOI: 10.1117/12.782174.

Interleukin-2 signalling is modulated by a labile disulfide bond in the CD132 chain of its receptor. Clive Metcalfe, Peter Cresswell and A. Neil Barclay. Open Biology. 2012, 2 (1). January 11, 2012. DOI: 10.1098/rsob.110036.

Electrochemical Template Synthesis of Multisegment Nanowires:  Fabrication and Protein Functionalization. Bridget Wildt, Prashant Mali, and Peter C. Searson. Langmuir. 2006, 22 (25), pp 10528–10534. September 8, 2006. 10.1021/la061184j.

Targeted delivery of a photosensitizer to Aggregatibacter actinomycetemcomitans biofilm. Suci P, Kang S, Gmür R, Douglas T, Young M.. Antimicrobial Agents and Chemotherapy. 2010, 54 (6) pp 2489-2496. April 12, 2010. 10.1128/AAC.00059-10.

The post Biotin-dPEG®₃-MAL appeared first on VectorLabs.

]]> Biotin-dPEG®₁₁-MAL https://staging.vectorlabs.com/products/biotin-dpeg11-mal/ Sat, 24 Feb 2024 02:05:19 +0000 https://staging.vectorlabs.com/?post_type=product&p=35267 Biotin-dPEG®11-MAL, product number QBD-10195, is a medium-length, discrete PEG (dPEG®) biotinylation reagent that reacts with free thiols through the maleimido group in the pH range of 6.5 – 7.5, forming a stable thioether linkage.

Description

Biotin-dPEG®11-MAL, product number QBD-10195, is one of three products containing a biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other end. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. This product provides a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between the biotin and the maleimide. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include magnetic bead-based assays, assays to quantitate reducible thiols in proteins, atomic force microscopy, and nanoparticle applications, among others.

To use Biotin-dPEG®11-MAL, the product should be dissolved in dry solvent and then added to an aqueous solution of the compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent to use for this purpose. For biomolecules (e.g., antibodies), care should be taken to keep the amount of organic solvent to a minimum so as not to denature the biomolecule or cause salt precipitation.

Specifications

Unit Size 25 mg, 100 mg
Molecular Weight 922.09; single compound
Chemical formula C₄₁H₇₁N₅O₁₆S
CAS 1334172-60-9
Purity > 98%
Spacers dPEG® Spacer is 43 atoms and 50.5 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₁₁-MAL appeared first on VectorLabs.

]]>

Biotin-dPEG®11-MAL, product number QBD-10195, is a medium-length, discrete PEG (dPEG®) biotinylation reagent that reacts with free thiols through the maleimido group in the pH range of 6.5 – 7.5, forming a stable thioether linkage.

Description

Biotin-dPEG®11-MAL, product number QBD-10195, is one of three products containing a biotin on one end of a dPEG® linker and a maleimidopropyl moiety on the other end. The maleimide group is a useful, highly popular reactive group for conjugating to free thiol groups in biomolecules. This product provides a short-chain, single molecular weight, discrete PEG (dPEG®) spacer between the biotin and the maleimide. Maleimide groups can be conjugated to free thiol groups in the pH range from 6.5 – 7.5, forming a stable thioether linkage. The dPEG® spacer in this product increases the hydrophilicity of the conjugate.

This product has proven useful in a variety of applications that take advantage of the high streptavidin-biotin binding affinity. Such applications include magnetic bead-based assays, assays to quantitate reducible thiols in proteins, atomic force microscopy, and nanoparticle applications, among others.

To use Biotin-dPEG®11-MAL, the product should be dissolved in dry solvent and then added to an aqueous solution of the compound containing the free thiol. The water-miscible solvent N,N’-dimethylacetamide (DMAC) dried over 3Å molecular sieves is an excellent solvent to use for this purpose. For biomolecules (e.g., antibodies), care should be taken to keep the amount of organic solvent to a minimum so as not to denature the biomolecule or cause salt precipitation.

Specifications

Unit Size25 mg, 100 mg
Molecular Weight922.09; single compound
Chemical formulaC₄₁H₇₁N₅O₁₆S
CAS1334172-60-9
Purity> 98%
SpacersdPEG® Spacer is 43 atoms and 50.5 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

  1. Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.
  2. A magnetic bead-based protein kinase assay with dual detection techniques. Guangchang Zhou, Juliesta E. Sylvester, Ding Wua, Darren R. Veach , Stephen J. Kron. Analytical Biochemistry. 2011, 1 (408), pp 5-11, January 2011. DOI: 10.1016/j.ab.2010.08.034.
  3. An Assay to Quantitate Reducible Cysteines from Nanograms of GST-Fusion Proteins. Dixon J. Woodbury, Chris A. Rees, Ammon Thompson, Paul Meiners, April Adams. Analytical Biochemistry. 2011, 417 (2) pp 165-173. October 15, 2011. DOI: 10.1016/j.ab.2011.06.017.
  4. The rotation-coupled sliding of EcoRV. Jasmina Dikic, Carolin Menges, Samuel Clarke, Michael Kokkinidis, Alfred Pingoud, Wolfgang Wende and Pierre Desbiolles. Nucleic Acids Research. 2012, 40 (9), pp 4064-4070. January 12, 2012. DOI:10.1093/nar/gkr1309.
  5. Fluorescent Silica Nanoparticles with Well-Separated Intensity Distributions from Batch Reactions. Teresa Kao , Ferdinand Kohle, Kai Ma, Tangi Aubert , Alexander Andrievsky, and Ulrich Wiesner. Nano Letters. 2018, 18, pp 1305-1310. January 2, 2018. DOI: 10.1021/acs.nanolett.7b04978.
  6. TCR Triggering by pMHC Ligands Tethered on Surfaces via Poly(Ethylene Glycol) Depends on Polymer Length. Zhengyu Ma, David N. Lebard, Sharon M. Loverde, Kim A. Sharp, Michael L. Klein, Dennis E. Discher, Terri H. Finkel. PLoS ONE. 2014. November 10, 2014. DOI: 10.137/journal.pone.0112292.
  7. Supramolecular Assembly of Three-Dimensional Protein Networks. Fatima Omar Merza. UWSPACE. 2020. 9/2/2020.
  8. Cohesin mediates DNA loop extrusion by a “swing and clamp” mechanism. Benedikt W Bauer, Iain F Davidson, Daniel Canena, Gordana Wutz, Wen Tang, Gabriele Litos, Sabrina Horn, Peter Hinterdorfer, Jan-Michael Peters. Cell. 2021. Volume 184, Issue 21. October 07, 2021. DOI: https://doi.org/10.1016/j.cell.2021.09.016
  9. Structural determinants of the integrin transmembrane domain required for bidirectional signal transmission across the cell membrane. Zhengli Wang, Jieqing Zhu. Journal of Biological Chemistry. 2021. Volume 297, Issue 5. October 20, 2021. DOI: https://doi.org/10.1016/j.jbc.2021.101318

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]]> Biotin-PEG3-Maleimide https://staging.vectorlabs.com/products/biotin-peg3-maleimide/ Tue, 19 Sep 2023 19:27:51 +0000 https://staging.vectorlabs.com/?post_type=product&p=21968 Description

Biotin-PEG3-Maleimide enables simple and efficient biotinylation of antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group reacts specifically and efficiently with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. he hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution.

Specifications

Unit Size 25 mg, 100 mg, 1000mg
Molecular weight 569.67
Chemical composition C25H39N5O8S
CAS 1431618-70-0
Solubility DMSO, DMF
Purity >90%
Appearance Colorless to slightly yellow amorphous solid
Storage Conditions -20C
Shipping Conditions Ambient temperature

The post Biotin-PEG3-Maleimide appeared first on VectorLabs.

]]>

Description

Biotin-PEG3-Maleimide enables simple and efficient biotinylation of antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group reacts specifically and efficiently with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. he hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution.

Specifications

Unit Size25 mg, 100 mg, 1000mg
Molecular weight569.67
Chemical compositionC25H39N5O8S
CAS1431618-70-0
SolubilityDMSO, DMF
Purity>90%
AppearanceColorless to slightly yellow amorphous solid
Storage Conditions-20C
Shipping ConditionsAmbient temperature

Documents

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]]> ChromaLINK® Biotin Maleimide https://staging.vectorlabs.com/products/chromalink-biotin-maleimide-reagent/ Wed, 03 May 2023 04:21:45 +0000 https://staging.vectorlabs.com/?post_type=product&p=14810 Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. The maleimide group reacts with free thiol groups on biomolecules and surfaces, such as the hinge region of antibodies after mild reduction of sulfhydryl groups with TCEP or DTT.

Specifications

Label/Modifier Type biotin
Reactivity Streptavidin
Recommended Storage Desiccated: -15° to -25°C
Applications Antibody Labeling, Aptamers
Other Name(s) SoluLINK Bioconjugation

The post ChromaLINK® Biotin Maleimide appeared first on VectorLabs.

]]>

Description

ChromaLINK Biotin contains a UV-traceable chromophore based on ChromaLINK technology to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple UV scan, you can quantify biotin incorporation and ensure reproducible production of consistent batches. The maleimide group reacts with free thiol groups on biomolecules and surfaces, such as the hinge region of antibodies after mild reduction of sulfhydryl groups with TCEP or DTT.

Specifications

Label/Modifier Typebiotin
ReactivityStreptavidin
Recommended StorageDesiccated: -15° to -25°C
ApplicationsAntibody Labeling, Aptamers
Other Name(s)SoluLINK Bioconjugation

Documents

Technical Information

 

Introduction to ChromaLINK Labeling Technology

ChromaLINK Biotin Maleimide incorporates UV-traceable biotin onto thiol containing proteins, peptides and/or antibodies. ChromaLINK Biotin Maleimide has been engineered to include many novel features. As illustrated in Figure 1, the molecule’s structure contains a bis-aryl hydrazone chromophore (a), linked by a PEG3 linker arm (b), to biotin (c). This reagent permits direct spectroscopic quantification of incorporated biotin. The extended PEG3 linker preserves biotin/streptavidin affinity and maintains protein solubility after modification while the maleimide functional group (d), efficiently modifies thiols in aqueous buffers.

B 1012 F1

Figure 1. Molecular structure of ChromaLINK Biotin Maleimide

Labeling of proteins with ChromaLINK Biotin eliminates the need to carry out cumbersome and time-consuming HABA assays often employed to quantify biotin incorporation. Instead, biotin incorporation is quantified by means of a simple spectrophotometric measurement at two wavelengths (A280 / A354). Typical labeling results are illustrated in Figure 2 by spectral overlay scans of four samples. As illustrated, bovine Serum Albumin (100 ul @ 1 mg/ml) was labeled at 0, 5, 10, and 20 mole equivalents using ChromaLINK Biotin Maleimide. Spectral analysis illustrates how easy it is to visualize, confirm, and quantify biotin incorporation.

B 1012 F2 jpg

Figure 2. Superimposed spectra of BSA biotinylated using ChromaLINK Biotin Maleimide. Various biotin-to-protein mole equivalents (5X, 10X and 20X) were used. Note the UV-signature at 354nm indicating incorporation of biotin. All spectra were scanned on a Molecular Dynamics SpectraMax PlusTM UV-VIS plate reader (220-420 nm).

 
 

Legal Information

SoluLINK® Bioconjugation For Research Use Only. Not for use in diagnostic procedures. For additional licensing restrictions, please see the license agreement at vectorlabs.com/solulink-research-license.

Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

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]]> Biotin (Long Arm) Maleimide https://staging.vectorlabs.com/products/biotin-long-arm-maleimide/ Sat, 29 Apr 2023 17:46:04 +0000 https://staging.vectorlabs.com/?post_type=product&p=13315 Description

Biotin (Long Arm) Maleimide is designed for biotinylating proteins, nucleic acids or other molecules containing one or more thiol groups. The presence of the 6-aminohexanoate spacer arm between the maleimide group and biotin reduces the possibility of steric hinderance. Molecules to be labeled with Biotin (Long Arm) Maleimide require free thiol groups. In the case of proteins, Biotin (Long Arm) Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).

Specifications

 
Unit Size 12 mg
Target for Labeling DNA, Oligonucleotides, PNA, Protein, RNA
Recommended Storage Storage in solution not recommended. – 20°C to – 80 °C.
Molecular Weight 479.6
Required Reactive Group Thiol Group
Tag/Group Incorporated Biotin
Safety Title WARNING: NA – www.P65Warnings.ca.gov

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Description

Biotin (Long Arm) Maleimide is designed for biotinylating proteins, nucleic acids or other molecules containing one or more thiol groups. The presence of the 6-aminohexanoate spacer arm between the maleimide group and biotin reduces the possibility of steric hinderance. Molecules to be labeled with Biotin (Long Arm) Maleimide require free thiol groups. In the case of proteins, Biotin (Long Arm) Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as primary amines with compounds like Traut’s reagent (2-iminothiolane).

Specifications

 
Unit Size12 mg
Target for LabelingDNA, Oligonucleotides, PNA, Protein, RNA
Recommended StorageStorage in solution not recommended. – 20°C to – 80 °C.
Molecular Weight479.6
Required Reactive GroupThiol Group
Tag/Group IncorporatedBiotin
Safety TitleWARNING: NA – www.P65Warnings.ca.gov

Documents

Citations

Technical Information

Biotin (Long Arm) Maleimide reacts readily under mild acid or neutral pH conditions in aqueous solution with free sulfhydryl groups such as cysteine residues or thiol groups introduced chemically into proteins, nucleic acids, or other macromolecules.

For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the 5′ EndTag™ or 3′ EndTag™ nucleic acid labeling systems.

Because of the extraordinary affinity of avidin and streptavidin to biotin and the many biotin-avidin/streptavidin systems available, the biotin label is ideal for a variety of applications including in situ hybridization, blotting, and affinity binding.

The spacer arm in this derivative insures that the biotin is fully accessible to avidin or streptavidin detection systems.

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