Biotin Azide – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:29 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin Azide – VectorLabs https://staging.vectorlabs.com 32 32 Biotin-dPEG®₇-azide https://staging.vectorlabs.com/products/biotin-dpeg7-azide/ Sat, 24 Feb 2024 02:13:25 +0000 https://staging.vectorlabs.com/?post_type=product&p=37010 Biotin-dPEG®7-azide, product number QBD-10825, is a water-soluble biotinylation product functionalized with azide for click chemistry use on a short (27 atoms, 30.7 Å) single molecular weight, discrete PEG (dPEG®) chain. This product enables the biotinylation of molecules alkyne-containing molecules and surfaces using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Moreover, the hydrophilic discrete PEG spacer makes biotin highly water soluble, reducing or eliminating aggregation and precipitation of biotin-labeled biomolecules triggered by hydrophobic interactions. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®7-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 620.32; single compound
Chemical formula C₂₆H₄₈N₆O₉S
CAS 1334172-75-6
Purity > 98%
Spacers dPEG® Spacer is 27 atoms and 30.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₇-azide appeared first on VectorLabs.

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Description

Biotin-dPEG®7-azide, product number QBD-10825, is a water-soluble biotinylation product functionalized with azide for click chemistry use on a short (27 atoms, 30.7 Å) single molecular weight, discrete PEG (dPEG®) chain. This product enables the biotinylation of molecules alkyne-containing molecules and surfaces using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Moreover, the hydrophilic discrete PEG spacer makes biotin highly water soluble, reducing or eliminating aggregation and precipitation of biotin-labeled biomolecules triggered by hydrophobic interactions. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®7-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®7-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®7-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®7-azide.

Specifications

Unit Size100mg, 1000mg
Molecular Weight620.32; single compound
Chemical formulaC₂₆H₄₈N₆O₉S
CAS1334172-75-6
Purity> 98%
SpacersdPEG® Spacer is 27 atoms and 30.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.

Palmitoylation of superoxide dismutase 1(SOD1) is increased for familial ALS-linked SOD1 mutants. Sarah E. Antinone, Ghanashyam D. Ghadge, TuKiet T. Lam, Lijun Wang, Raymond P. Roos and William N. Green. J. Biol. Chem. 2013, 288 (49) pp 1-25. June 12, 2013. DOI: 10.1074/jbc.M113.487231.

Herpes Simplex Virus 2 Infection Impacts Stress Granule Accumulation. Renée L. Finnen, Kyle R. Pangka, and Bruce W. Banfield. J. Virol. 2012, 86 (15) pp 8119–8130. August 1, 2012. doi:10.1128/JVI.00313-12.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.

Polymer Therapeutics with a Coiled Coil Motif Targeted against Murine BCL1 Leukemia. Robert Pola, Richard Laga, Karel Ulbrich, Irena Sieglová, Vlastimil Král, Milan Fábry, Martina Kabešová, Marek Kovář, and Michal Pechar. Biomacromolecules. 2013, 14 (3) pp 881–889. February 1, 2013. DOI: 10.1021/bm3019592.

Sequential Nucleophilic Substitutions Permit Orthogonal Click Functionalization of Multicomponent PEG Brushes. Jin Sha, Ethan S. Lippmann, Jason McNulty, Yulu Ma, and Randolph S. Ashton. Biomacromolecules. 2013, 14 (9) pp 3294–3303. August 13, 2013. DOI: 10.1021/bm400900r.

Detection of O -GlcNAc Modi fi cations on Cardiac Myo fi lament Proteins. Genaro A. Ramirez-Correa , Isabel Martinez Ferrando , Gerald Hart and Anne Murphy. Methods in Molecular Biology. 2013, 1005 (13) pp 157-168. January 1, 2013. DOI: 10.1007/978-1-62703-386-2_13.

The post Biotin-dPEG®₇-azide appeared first on VectorLabs.

]]> Biotin-dPEG®₂₃-azide https://staging.vectorlabs.com/products/biotin-dpeg23-azide/ Sat, 24 Feb 2024 02:13:05 +0000 https://staging.vectorlabs.com/?post_type=product&p=36844 Biotin-dPEG®23-azide, product number QBD-10787, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a long (73 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®23-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®23-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Vector Laboratories to create new compounds tailored to the customer’s specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).

As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1325.60; single compound
Chemical formula C₅₈H₁₁₂N₆O₂₅S
CAS 956494-20-5
Purity > 98%
Spacers dPEG® Spacer is 73 atoms and 87.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₂₃-azide appeared first on VectorLabs.

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Description

Biotin-dPEG®23-azide, product number QBD-10787, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a long (73 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®23-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®23-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

Biotin-dPEG®23-azide has numerous uses. Through protein engineering, non-natural amino acids with alkyne side chains can be incorporated into distinct locations on proteins, thereby enabling site-specific, reproducible biotinylation of proteins. Likewise, synthetic peptides can be constructed that allow azide-alkyne click chemistry-driven biotinylation of the peptides. Also, surfaces functionalized with alkyne groups can be biotinylated using click chemistry. Furthermore, this product can be modified with other products from Vector Laboratories to create new compounds tailored to the customer’s specific needs. For example, in a 2018 study published in the journal Langmuir, researchers from The BioDesign Institute at Arizona State University used Biotin-dPEG®23-azide to construct a Y-shaped, bivalent, bis-biotin structure with a rigid core to probe the interaction of biotin and streptavidin using both atomic force microscopy (AFM) and surface plasmon resonance (SPR).

As a biotinylation reagent, Biotin-dPEG®23-azide functions like other biotinylation reagents. For example, pull-down assays, affinity purification, ELISA and other plate-type assays, and supramolecular construction can employ Biotin-dPEG®23-azide successfully. Many other applications using Biotin-dPEG®23-azide are possible.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1325.60; single compound
Chemical formulaC₅₈H₁₁₂N₆O₂₅S
CAS956494-20-5
Purity> 98%
SpacersdPEG® Spacer is 73 atoms and 87.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w

The post Biotin-dPEG®₂₃-azide appeared first on VectorLabs.

]]> Biotin-dPEG®₁₁-azide https://staging.vectorlabs.com/products/biotin-dpeg11-azide/ Sat, 24 Feb 2024 02:13:02 +0000 https://staging.vectorlabs.com/?post_type=product&p=36812 Biotin-dPEG®11-azide, product number QBD-10784, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a medium-length (40 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®11-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®11-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®11-azide. For instance, in a novel use of QBD-10784, Biotin-dPEG®11-azide was employed in a pull-down assay to identify electrophiles in signaling pathways in the worm Caenorhabditis elegans.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 796.97; single compound
Chemical formula C₃₄H₆₄N₆O₁₃S
CAS 956494-20-5
Purity > 98%
Spacers dPEG® Spacer is 40 atoms and 50.4 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC or DMSO.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post Biotin-dPEG®₁₁-azide appeared first on VectorLabs.

]]>

Description

Biotin-dPEG®11-azide, product number QBD-10784, is a water-soluble biotinylation product functionalized with azide for click chemistry use, on a medium-length (40 atoms) single molecular weight, discrete PEG (dPEG®) chain. This product enables biotinylation of molecules using either metal-catalyzed (CuAAC, RuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) chemistry. Consequently, these products allow new types of biotinylated constructs.

Because Biotin-dPEG®11-azide is a single molecular weight dPEG® product, analysis of the resulting conjugates is simplified. The researcher using this product does not have to analyze a variety of PEG chain lengths and molecular weights. Instead, the well-defined dPEG® product will yield predictable, identifiable conjugates. When using Biotin-dPEG®11-azide with proteins or peptides engineered to contain alkyne side chains at specific locations, both the number and location of conjugation sites are predictable.

As a biotinylation reagent, Biotin-dPEG®11-azide works in various applications. Pull-down assays, affinity purification, plate-type assays such as ELISA, and supramolecular construction can all incorporate Biotin-dPEG®11-azide. For instance, in a novel use of QBD-10784, Biotin-dPEG®11-azide was employed in a pull-down assay to identify electrophiles in signaling pathways in the worm Caenorhabditis elegans.

Specifications

Unit Size100mg, 1000mg
Molecular Weight796.97; single compound
Chemical formulaC₃₄H₆₄N₆O₁₃S
CAS956494-20-5
Purity> 98%
SpacersdPEG® Spacer is 40 atoms and 50.4 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC or DMSO.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 2nd Edition, Elsevier Inc., Burlington, MA 01803, April, 2008 (ISBN-13: 978-0-12-370501-3; ISBN-10: 0-12-370501-0), and specifically see pp. 722-724.

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Cardiovascular Small Heat Shock Protein HSPB7 Is a Kinetically Privileged Reactive Electrophilic Species (RES) Sensor. Sanjna L. Surya, Marcus J. C. Long, Daniel A. Urul, Yi Zhao, Emily J. Mercer, Islam M. Elsaid, Todd Evans, and Yimon Aye. ACS Chemical Biolody. 2018, 13, pp 1824-1831. February 3, 2018. DOI: 10.1021/acschembio.7b00925.

Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens. Shixian Lin, Zhenrun Zhang, Hao Xu, Lin Li, She Chen, Jie Li, Ziyang Hao, and Peng R. Chen. J. Am. Chem. Soc. 2011, 133 (50), pp 20581–20587. November 15, 2011. DOI: 10.1021/ja209008w.

Precision Electrophile Tagging in Caenorhabditis elegans. Marcus J. C. Long, Daniel A. Urul, Shivansh Chawla, Hong-YuLin, Yi Zhao, Joseph A. Haegele, Yiran Wang, and Yimon Aye. Biochemistry. 2018, 57 (2), pp 216-220. August 31, 2017. DOI: 10.1021/acs.biochem.7b00642.

Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication. Akiko Kumagai, William G. Dunphy. Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. 2020. Volume 32, Issue 12. 9/22/2020. http://doi.org/10.1016/j.celrep.2020.108178

Function-guided proximity mapping unveils electrophilic-metabolite sensing by proteins not present in their canonical locales. Yi Zhao, Pierre A Miranda Herrera, Dalu Chang, Romain Hamelin, Marcus J C Long, Yimon Aye. PNAS Biochemistry. 2022. January 26, 2022. https://doi.org/10.1073/pnas.2120687119

The post Biotin-dPEG®₁₁-azide appeared first on VectorLabs.

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