Biotin Acid – VectorLabs https://staging.vectorlabs.com From linker design and synthesis, bioconjugated design and manufacturing, biomolecule labeling and functionalization, and detection system and solutions, our team is here to help you move forward, with impact. Wed, 22 Oct 2025 17:19:02 +0000 en-US hourly 1 https://wordpress.org/?v=6.8.3 https://staging.vectorlabs.com/wp-content/uploads/2024/11/V-favicon-02-100x100.png Biotin Acid – VectorLabs https://staging.vectorlabs.com 32 32 dPEG®₄₈-biotin acid https://staging.vectorlabs.com/products/dpeg48-biotin-acid/ Sat, 24 Feb 2024 02:13:02 +0000 https://staging.vectorlabs.com/?post_type=product&p=36804 dPEG®48 biotin acid, product number QBD-10776, is a biotinylation reagent containing a long (157 atoms), single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®48 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10776 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®48 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®48-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 2500.99; single compound
Chemical formula C₁₁₂H₂₁₈N₄O₅₃S
CAS 721431-18-1
Purity > 98%
Spacers dPEG® Spacer is 157 atoms and 187.8 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post dPEG®₄₈-biotin acid appeared first on VectorLabs.

]]>

Description

dPEG®48 biotin acid, product number QBD-10776, is a biotinylation reagent containing a long (157 atoms), single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®48 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10776 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®48 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®48-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size100mg, 1000mg
Molecular Weight2500.99; single compound
Chemical formulaC₁₁₂H₂₁₈N₄O₅₃S
CAS721431-18-1
Purity> 98%
SpacersdPEG® Spacer is 157 atoms and 187.8 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.

Sensitive Detection of Small Molecule–Protein Interactions on a Metal – Insulator – Metal Label-Free Biosensing Platform Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Chemistry Asian Journal. 2012, 8 (7), pp 1867-1874. August 2012. DOI: 10.1002/asia.201200138.

Prion protein 90-231 contains a streptavidin-binding motif. Thurid Boetel, Steffen Bade, Marcus Alexander Schmidt, Andreas Frey. Biochemical and Biophysical Research Communications. 2006, 349 (1) pp 296-302. October 13, 2006. DOI: 10.1016/j.bbrc.2006.08.041.

Macrocycles That Inhibit the Binding between Heat Shock Protein 90 and TPR-Containing Proteins. Veronica C. Ardi, Leslie D. Alexander, Victoria A. Johnson, and Shelli R. McAlpine. ACS Chem. Biol. 2011, 6 (12), pp 1357–1366. September 27, 2011. DOI: 10.1021/cb200203m.

Measurement of Inflammatory Cytokine Secretion from Human Monocytes after Inflammasome Activation. Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara. Royal Society of Chemistry. 2012, 978-0-9798064-5-2 pp 1012-1014. November 1, 2012. DOI: 12CBMS-0001.

The post dPEG®₄₈-biotin acid appeared first on VectorLabs.

]]> dPEG®₂₄-biotin acid https://staging.vectorlabs.com/products/dpeg24-biotin-acid/ Sat, 24 Feb 2024 02:12:59 +0000 https://staging.vectorlabs.com/?post_type=product&p=36788 dPEG®24 biotin acid, product number QBD-10773, is a biotinylation reagent containing a single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The dPEG® linker length is 76 atoms. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®24 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules, even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10773 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®24 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®24-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size 100mg, 1000mg
Molecular Weight 1372.65; single compound
Chemical formula C₆₁H₁₁₇N₃O₂₈S
CAS 721431-18-1
Purity > 97%
Spacers dPEG® Spacer is 76 atoms and 95.7 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post dPEG®₂₄-biotin acid appeared first on VectorLabs.

]]>

Description

dPEG®24 biotin acid, product number QBD-10773, is a biotinylation reagent containing a single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The dPEG® linker length is 76 atoms. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®24 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules, even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10773 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®24 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®24-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size100mg, 1000mg
Molecular Weight1372.65; single compound
Chemical formulaC₆₁H₁₁₇N₃O₂₈S
CAS721431-18-1
Purity> 97%
SpacersdPEG® Spacer is 76 atoms and 95.7 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

 



Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.

Sensitive Detection of Small Molecule–Protein Interactions on a Metal – Insulator – Metal Label-Free Biosensing Platform Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Chemistry Asian Journal. 2012, 8 (7), pp 1867-1874. August 2012. DOI: 10.1002/asia.201200138.

Prion protein 90-231 contains a streptavidin-binding motif. Thurid Boetel, Steffen Bade, Marcus Alexander Schmidt, Andreas Frey. Biochemical and Biophysical Research Communications. 2006, 349 (1) pp 296-302. October 13, 2006. DOI: 10.1016/j.bbrc.2006.08.041.

Macrocycles That Inhibit the Binding between Heat Shock Protein 90 and TPR-Containing Proteins. Veronica C. Ardi, Leslie D. Alexander, Victoria A. Johnson, and Shelli R. McAlpine. ACS Chem. Biol. 2011, 6 (12), pp 1357–1366. September 27, 2011. DOI: 10.1021/cb200203m.

Measurement of Inflammatory Cytokine Secretion from Human Monocytes after Inflammasome Activation. Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara. Royal Society of Chemistry. 2012, 978-0-9798064-5-2 pp 1012-1014. November 1, 2012. DOI: 12CBMS-0001.

The post dPEG®₂₄-biotin acid appeared first on VectorLabs.

]]> dPEG®₄-biotin acid https://staging.vectorlabs.com/products/dpeg4-biotin-acid/ Sat, 24 Feb 2024 02:07:01 +0000 https://staging.vectorlabs.com/?post_type=product&p=35319 "dPEG®4 biotin acid, product number QBD-10199, is a biotinylation reagent containing a single molecular weight tetraethylene glycol (PEG) spacer containing biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®4 biotin acid can be dissolved both in water or aqueous buffer and in organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10199 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®4 biotin acid is slightly longer than hydrophobic LC-biotin and demonstrates superior performance over LC-biotin due to its flexiblity and water solubility.

Product number QBD-10199 is the precursor product of our popular products QBD-10198, NHS-dPEG®4-biotin, and QBD-10008, Biotin-dPEG®4-TFP ester. Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures."

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 491.60; single compound
Chemical formula C₂₁H₃₇N₃O₈S
CAS 721431-18-1
Purity > 98%
Spacers dPEG® Spacer is 16 atoms and 19.2 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post dPEG®₄-biotin acid appeared first on VectorLabs.

]]>

Description

dPEG®4 biotin acid, product number QBD-10199, is a biotinylation reagent containing a single molecular weight tetraethylene glycol (PEG) spacer containing biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®4 biotin acid can be dissolved both in water or aqueous buffer and in organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10199 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®4 biotin acid is slightly longer than hydrophobic LC-biotin and demonstrates superior performance over LC-biotin due to its flexiblity and water solubility.

Product number QBD-10199 is the precursor product of our popular products QBD-10198, NHS-dPEG®4-biotin, and QBD-10008, Biotin-dPEG®4-TFP ester. Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight491.60; single compound
Chemical formulaC₂₁H₃₇N₃O₈S
CAS721431-18-1
Purity> 98%
SpacersdPEG® Spacer is 16 atoms and 19.2 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Macrocycles That Inhibit the Binding between Heat Shock Protein 90 and TPR-Containing Proteins. Veronica C. Ardi, Leslie D. Alexander, Victoria A. Johnson, and Shelli R. McAlpine. ACS Chem. Biol. 2011, 6 (12), pp 1357–1366. September 27, 2011. DOI: 10.1021/cb200203m.

Sensitive Detection of Small Molecule–Protein Interactions on a Metal – Insulator – Metal Label-Free Biosensing Platform. Amir Syahir, Kotaro Kajikawa and Hisakazu Mihara. Chemistry Asian Journal. 2012, 8 (7), pp 1867-1874. August 2012. DOI: 10.1002/asia.201200138.

Surface Functionalization Using Catalyst-Free Azide-Alkyne Cycloaddition. Alexander Kuzmin, Andrei Poloukhtine, Margreet A. Wolfert, and Vladimir V. Popik. Bioconjugate Chem. 2010, 21 (11) pp 2076–2085. October 21, 2010. DOI: 10.1021/bc100306u.


Measurement of Inflammatory Cytokine Secretion from Human Monocytes after Inflammasome Activation. Yoshitaka Shirasaki, Asahi Nakahara, Nanako Shimura, Kazushi Izawa, Nobutake Suzuki, Mai Yamagishi, Jun Mizuno, Tetsushi Sekiguchi, Ryuta Nishikomori, Shuichi Shoji, Osamu Ohara. Royal Society of Chemistry. 2012, 978-0-9798064-5-2 pp 1012-1014. November 1, 2012. DOI: 12CBMS-0001.

Prion protein 90-231 contains a streptavidin-binding motif. Thurid Boetel, Steffen Bade, Marcus Alexander Schmidt, Andreas Frey. Biochemical and Biophysical Research Communications. 2006, 349 (1) pp 296-302. October 13, 2006. DOI: 10.1016/j.bbrc.2006.08.041.

Real-time single-cell imaging of protein secretion. Yoshitaka Shirasaki, Mai Yamagishi, Nobutake Suzuki, Kazushi Izawa, Asahi Nakahara, Jun Mizuno, Shuichi Shoji, Toshio Heike, Yoshie Harada, Ryuta Nishikomori and Osamu Ohara. Scientific Reports. 2014, 4 (4736) pp 1-16. April 22, 2014. DOI: 10.1038/srep04736.

Synthesis of Three-dimensional Polymer Nanostructures via Chemical Vapor Deposition. Kenneth Chang. Dissertations and Theses (Ph.D. and Master’s). 2017, pp iv-157. 10/5/2017.

A high-resolution real-time quantification of astrocyte cytokine secretion under shear stress for investigating hydrocephalus shunt failure. Fatemeh Khodadadei, Allen P. Liu & Carolyn A. Harris. Communications Biology. 2021. 4, Article number: 387 (2021). 03/23/2021. DOI: 10.1038/s42003-021-01888-7

Quantitative live-cell imaging of secretion activity reveals dynamic immune responses. Mai Yamagishi, Kaede Miyata, Takashi Kamatani, Hiroki Kabata, Rie Baba, Yumiko Tanaka, Nobutake Suzuki, Masako Matsusaka, Yasutaka Motomura, Tsuyoshi Kiniwa, Satoshi Koga, Keisuke Goda, Osamu Ohara, Takashi Funatsu, Koichi Fukunaga, Kazuyo Moro, Sotaro Uemura, Yoshitaka Shirasaki. bioRxiv. 2022. January 10, 2022. https://doi.org/10.1101/2022.02.09.479547

The post dPEG®₄-biotin acid appeared first on VectorLabs.

]]> dPEG®₁₂-biotin acid https://staging.vectorlabs.com/products/dpeg12-biotin-acid/ Sat, 24 Feb 2024 02:06:57 +0000 https://staging.vectorlabs.com/?post_type=product&p=35297 "dPEG®12 biotin acid, product number QBD-10197, is a biotinylation reagent containing a single molecular weight polyethylene glycol (PEG) spacer containing biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®12 biotin acid can be dissolved both in water or aqueous buffer and in organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10197 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®12 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®12-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Product number QBD-10197 is the precursor product of our popular products QBD-10198, NHS-dPEG®12-biotin, and QBD-10008, Biotin-dPEG®12-TFP ester. Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures."

Specifications

Unit Size 100 mg, 1000 mg
Molecular Weight 844.02; single compound
Chemical formula C₃₇H₆₉N₃O₁₆S
CAS 948595-11-7
Purity > 98%
Spacers dPEG® Spacer is 40 atoms and 47.6 Å
Shipping Ambient
Typical solubility properties (for additional information contact Customer Support) Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

The post dPEG®₁₂-biotin acid appeared first on VectorLabs.

]]>

Description

dPEG®12 biotin acid, product number QBD-10197, is a biotinylation reagent containing a single molecular weight polyethylene glycol (PEG) spacer containing biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.

Amphiphilic dPEG®12 biotin acid can be dissolved both in water or aqueous buffer and in organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10197 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®12 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®12-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.

Product number QBD-10197 is the precursor product of our popular products QBD-10198, NHS-dPEG®12-biotin, and QBD-10008, Biotin-dPEG®12-TFP ester. Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.

Specifications

Unit Size100 mg, 1000 mg
Molecular Weight844.02; single compound
Chemical formulaC₃₇H₆₉N₃O₁₆S
CAS948595-11-7
Purity> 98%
SpacersdPEG® Spacer is 40 atoms and 47.6 Å
ShippingAmbient
Typical solubility properties (for additional information contact Customer Support)Methylene chloride, Acetontrile, DMAC, DMSO or water.
Storage and handling-20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure.

Documents

References

Greg T. Hermanson, Bioconjugate Techniques, 3rd Edition, Elsevier, Waltham, MA 02451, 2013, ISBN 978-0-12-382239-0; See Chapter 18, Discrete PEG Reagents, pp. 787-821, for a full overview of the dPEG® products.

Sphere-Like Protein-Glycopolymer Nanostructures Tailored by Polyassociation. Franka Ennen, Philipp Fenner, Susanne Boye, Albena Lederer, Hartmut Komber, Brigitte Voit, and Dietmar Appelhans. Biomacromolecules. 2015, 16 (12) pp 3731-4032. December 1, 2015. DOI: 10.1021/acs.biomac.5b00975.

Sialylation of IgG Fc domain impairs complement-dependent cytotoxicity. Isaak Quast, Christian W. Keller, Michael A. Maurer, John P. Giddens, Bjorn Tackenberg, Lai-Xi Wang, Christian Munz, Falk Nimmerjahn, Marinos C. Dalakas and Jan D. Lunemann. The Journal of Clinical Investigation. 2015, August 25, 2015. DOI: 10.1172/JCI82695.

NV Center Electron Paramagnetic Resonance of a Single Nanodiamond Attached to an Individual Biomolecule. Richelle M. Teeling-Smith, Young Woo Jung, Nicolas Scozzaro, Jeremy Cardellino, Isaac Rampersaud, Justin A North, Marek Simon, Vidya P. Bhallamudi, Arfaan Rampersaud, Ezekiel Johnston-Halperin, Michael G. Poirier, and P. Chris Hammel. Mesoscale and Nanoscale Physics. 2015. November 21, 2015. DOI: arXiv:1511.06831v1.

Magnetic Separation of Melenoma-Specific Cytoxic T Lymphocytes from a Vaccinated Melanoma Patients’s Blood Using MHC/Peptide Complex-Conjugated Bacterial Magnetic Particles, Masayuki Takahashi, Yasuto Akiyama, Junpei Ikezumi, Takeshi Nagata, Tomoko Yoshino, Akira Iizuka, Ken Yamaguchi, and Tadashi Matsunaga. Bioconjugate Chemistry. 2009, 20 (2) pp 304–309. January 14, 2009. DOI: 10.1021/bc800398d.

Delivery of NADPH-Cytochrome P450 Reductase Antisense Oligos Using Avidin-Biotin Approach. Venkateswaren C. Pillai, Rekha Yesudas, Imam H. Shaik, Thomas J. Thekkumkara, Ulrich Bickel, Kalkunte S. Srivenugopal, and Reza Mehvar. Bioconjugate Chemistry. 2010, 21 (2) pp 203–207. January 11, 2010. DOI: 10.1021/bc900449b.

Trafficking of immature _F508-CFTR to the plasma membrane and its detection by biotinylation. Yishan Luo, Ken McDonald and John W. Hanrahan. Biochem J. 2009, 419 pp 211–219. December 8, 2008. doi:10.1042/BJ20081869.

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